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The map label for this gene is rfbD

Identifier: 45657973

GI number: 45657973

Start: 2567634

End: 2568554

Strand: Reverse

Name: rfbD

Synonym: LIC12125

Alternate gene names: 45657973

Gene position: 2568554-2567634 (Counterclockwise)

Preceding gene: 45657974

Following gene: 45657972

Centisome position: 60.05

GC content: 37.13

Gene sequence:

>921_bases
ATGATTTATTATACTGGAAAAAACGGTCAGTTAGGTTGGGAATTGGCCGAAAGATTTAAATCTAATGGATTGGAAGCAGT
AGGATTTGGTAGGGAAGAATGGGATCTTGCAGATCTTGATTCTGCCGCACGAATTTTGAAGGATTCTCCTCGGATTTTAG
TTCATTGTGGAGCTTATACTGCGGTAGATAAGGCAGAGTCCGACTCAGAGAACGCTTACAAAATCAATTCGTTATCTGTA
AAAAAAATTTCGGAAGAATGTCTAAAAAGAAAAATCCATTTAATTTATATATCCACTGATTTTGTGTTTGATGCAAATTC
GGAAACGATCGGTGATACGATTCGTTTTTGGAAACCGGATTCTACACTTTCTCCGAAAGGAATCTACGGATTGTCAAAGG
CGGAAGGGGAAAAGTGGATTCGAAATACGTTTGCGAATTCTAAACAAGCAAATATAGTTCGTACCAGTTGGGTATATTCC
TCTCATGGAAATAATTTTCCAAAAACAATTGTGAAACTTTTACAAGATTCCAATCGTCATGAATTAAGAGTGATCGAAGA
TCAATTGGGAAGACCTACTTGGGCTGGTAGGCTTGCGGATTTTATAATATTCTTAATTTATGGAATTCTAAAAGGAGAAT
CTTATCCTGAGATTCTACATTTTTCTAACTCAGGAGTTGCGAGCTGGTACGATTTTGCACTTGCGGTTCGGGATTTTTCA
CATTCTTTTTCATTGATAGAAAATTTGAAGCCGATTTATCCAATTCCTACGGAAGGTTATCCAACACCTGCACCTAGGCC
TAGATATTCAATTTTAGATTTAGAAGAAACTCGAAAAATTTTTGGCGTTGTTCCGCATTGGAGAGAGGATTTAACCCTTT
GTCTGAAAGAACTTGCCGAAGTTTCCGGAAAAAAAGTATGA

Upstream 100 bases:

>100_bases
TTGGAAGACATGGTTGTCTGATTCTGAATTTATAGTATCTCAGAGGGACCAAGTGACTCCTTTTTTTGCAGACTTTAAAA
GTCCATTCGTATATTAGAAT

Downstream 100 bases:

>100_bases
AAAAAATTTTAGTTACGGGTGGAGCCGGTTTTATAGGTTCCAATTTTGTAAATCTCATCTTAAACGATACCAAAGAATAT
CAGGTAGTCGTATTCGATAA

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MIYYTGKNGQLGWELAERFKSNGLEAVGFGREEWDLADLDSAARILKDSPRILVHCGAYTAVDKAESDSENAYKINSLSV
KKISEECLKRKIHLIYISTDFVFDANSETIGDTIRFWKPDSTLSPKGIYGLSKAEGEKWIRNTFANSKQANIVRTSWVYS
SHGNNFPKTIVKLLQDSNRHELRVIEDQLGRPTWAGRLADFIIFLIYGILKGESYPEILHFSNSGVASWYDFALAVRDFS
HSFSLIENLKPIYPIPTEGYPTPAPRPRYSILDLEETRKIFGVVPHWREDLTLCLKELAEVSGKKV

Sequences:

>Translated_306_residues
MIYYTGKNGQLGWELAERFKSNGLEAVGFGREEWDLADLDSAARILKDSPRILVHCGAYTAVDKAESDSENAYKINSLSV
KKISEECLKRKIHLIYISTDFVFDANSETIGDTIRFWKPDSTLSPKGIYGLSKAEGEKWIRNTFANSKQANIVRTSWVYS
SHGNNFPKTIVKLLQDSNRHELRVIEDQLGRPTWAGRLADFIIFLIYGILKGESYPEILHFSNSGVASWYDFALAVRDFS
HSFSLIENLKPIYPIPTEGYPTPAPRPRYSILDLEETRKIFGVVPHWREDLTLCLKELAEVSGKKV
>Mature_306_residues
MIYYTGKNGQLGWELAERFKSNGLEAVGFGREEWDLADLDSAARILKDSPRILVHCGAYTAVDKAESDSENAYKINSLSV
KKISEECLKRKIHLIYISTDFVFDANSETIGDTIRFWKPDSTLSPKGIYGLSKAEGEKWIRNTFANSKQANIVRTSWVYS
SHGNNFPKTIVKLLQDSNRHELRVIEDQLGRPTWAGRLADFIIFLIYGILKGESYPEILHFSNSGVASWYDFALAVRDFS
HSFSLIENLKPIYPIPTEGYPTPAPRPRYSILDLEETRKIFGVVPHWREDLTLCLKELAEVSGKKV

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI11034825, Length=285, Percent_Identity=26.3157894736842, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI33519455, Length=285, Percent_Identity=26.3157894736842, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1788352, Length=302, Percent_Identity=36.0927152317881, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 34774; Mature: 34774

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYYTGKNGQLGWELAERFKSNGLEAVGFGREEWDLADLDSAARILKDSPRILVHCGAYT
CEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCH
AVDKAESDSENAYKINSLSVKKISEECLKRKIHLIYISTDFVFDANSETIGDTIRFWKPD
HHHCCCCCCCCCEEECCHHHHHHHHHHHHCCEEEEEEEEEEEEECCCCCCCCCEEEECCC
STLSPKGIYGLSKAEGEKWIRNTFANSKQANIVRTSWVYSSHGNNFPKTIVKLLQDSNRH
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCC
ELRVIEDQLGRPTWAGRLADFIIFLIYGILKGESYPEILHFSNSGVASWYDFALAVRDFS
CEEEHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHH
HSFSLIENLKPIYPIPTEGYPTPAPRPRYSILDLEETRKIFGVVPHWREDLTLCLKELAE
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHCCCCCHHHHHHHHHHHHHH
VSGKKV
HCCCCC
>Mature Secondary Structure
MIYYTGKNGQLGWELAERFKSNGLEAVGFGREEWDLADLDSAARILKDSPRILVHCGAYT
CEEEECCCCCCHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEECCCH
AVDKAESDSENAYKINSLSVKKISEECLKRKIHLIYISTDFVFDANSETIGDTIRFWKPD
HHHCCCCCCCCCEEECCHHHHHHHHHHHHCCEEEEEEEEEEEEECCCCCCCCCEEEECCC
STLSPKGIYGLSKAEGEKWIRNTFANSKQANIVRTSWVYSSHGNNFPKTIVKLLQDSNRH
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCC
ELRVIEDQLGRPTWAGRLADFIIFLIYGILKGESYPEILHFSNSGVASWYDFALAVRDFS
CEEEHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHH
HSFSLIENLKPIYPIPTEGYPTPAPRPRYSILDLEETRKIFGVVPHWREDLTLCLKELAE
HHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECHHHHHHHHCCCCCHHHHHHHHHHHHHH
VSGKKV
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]