Click here to switch to the map view.

The map label for this gene is leuD

Identifier: 45657686

GI number: 45657686

Start: 2215506

End: 2216126

Strand: Reverse

Name: leuD

Synonym: LIC11821

Alternate gene names: 45657686

Gene position: 2216126-2215506 (Counterclockwise)

Preceding gene: 45657687

Following gene: 45657685

Centisome position: 51.81

GC content: 38.97

Gene sequence:

>621_bases
ATGAAACCCTTTACTATATTAAATGGAATTGCCGCCTTACTGGACAGACCCAACGTGGATACGGATCAGATCATTCCAAA
ACAATTTTTACGGAAGATAGAAAGAACCGGTTTCGGAGTTCATCTGTTTCACGATTGGAGATACTTAGACGACGCGGGTA
CCAAACTCAATCCTGATTTTTCCCTCAATCAAGAACGATATAAGGGAGCTTCTATCCTTATCACCAGAGATAACTTTGGT
TGTGGATCTTCCAGAGAACACGCTCCTTGGGCTTTAGAAGACTACGGGTTTAGGGCAATCATTGCTCCTTCTTACGCGGA
TATTTTTTTCAACAACTGCTTTAAAAACGGAATGCTTCCAGTCATTTTAAAATCGGAAGAAGTAGAAGAGCTGTTCCATT
TAGTTTCGACTAACGTAGGAGCGAAAGTCATAGTGGATCTGGACAAACAAACTGTAACCGGACCGACTGGAAAAATATAT
TATTTTGAAGTGGATTCTTTTCGTAAATACTGTCTTTATAACGGACTTGATGACATAGGTCTAACTCTAAAACAAGAAAG
TAAGATTGGAGAGTTTGAAAAAAAGCAGAAAGAAGTTGAACCTTGGTTATACGCCATATAA

Upstream 100 bases:

>100_bases
AGAACCCATCTAGTAGGACCGGAAATGGCCGCCGCCGCGGCTATCGAAGGCCATTTTGTGGATATTCGAAACTGGAAATA
AAAAGGTAAATTAGAATATT

Downstream 100 bases:

>100_bases
TTCTACCGAAAATATATGTTTGATGAAATATCCCGTTCTATAGACGAATTCGGAAATAGTTTCCTTAGCGCGCTGAACAA
CATTCAGAAATCTTTTGGGC

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MKPFTILNGIAALLDRPNVDTDQIIPKQFLRKIERTGFGVHLFHDWRYLDDAGTKLNPDFSLNQERYKGASILITRDNFG
CGSSREHAPWALEDYGFRAIIAPSYADIFFNNCFKNGMLPVILKSEEVEELFHLVSTNVGAKVIVDLDKQTVTGPTGKIY
YFEVDSFRKYCLYNGLDDIGLTLKQESKIGEFEKKQKEVEPWLYAI

Sequences:

>Translated_206_residues
MKPFTILNGIAALLDRPNVDTDQIIPKQFLRKIERTGFGVHLFHDWRYLDDAGTKLNPDFSLNQERYKGASILITRDNFG
CGSSREHAPWALEDYGFRAIIAPSYADIFFNNCFKNGMLPVILKSEEVEELFHLVSTNVGAKVIVDLDKQTVTGPTGKIY
YFEVDSFRKYCLYNGLDDIGLTLKQESKIGEFEKKQKEVEPWLYAI
>Mature_206_residues
MKPFTILNGIAALLDRPNVDTDQIIPKQFLRKIERTGFGVHLFHDWRYLDDAGTKLNPDFSLNQERYKGASILITRDNFG
CGSSREHAPWALEDYGFRAIIAPSYADIFFNNCFKNGMLPVILKSEEVEELFHLVSTNVGAKVIVDLDKQTVTGPTGKIY
YFEVDSFRKYCLYNGLDDIGLTLKQESKIGEFEKKQKEVEPWLYAI

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=188, Percent_Identity=59.0425531914894, Blast_Score=237, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6321429, Length=202, Percent_Identity=52.4752475247525, Blast_Score=210, Evalue=1e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 23597; Mature: 23597

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPFTILNGIAALLDRPNVDTDQIIPKQFLRKIERTGFGVHLFHDWRYLDDAGTKLNPDF
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECEEEECCCCCEECCCC
SLNQERYKGASILITRDNFGCGSSREHAPWALEDYGFRAIIAPSYADIFFNNCFKNGMLP
CCCHHHCCCEEEEEEECCCCCCCCCCCCCEEHHCCCEEEEECCCHHHHHHHHHHHCCCEE
VILKSEEVEELFHLVSTNVGAKVIVDLDKQTVTGPTGKIYYFEVDSFRKYCLYNGLDDIG
EEECCHHHHHHHHHHHCCCCCEEEEEECCCEECCCCCEEEEEEHHHHHHHHHHCCCCHHC
LTLKQESKIGEFEKKQKEVEPWLYAI
EEEECCCCCCHHHHHHHHCCCEEECC
>Mature Secondary Structure
MKPFTILNGIAALLDRPNVDTDQIIPKQFLRKIERTGFGVHLFHDWRYLDDAGTKLNPDF
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECEEEECCCCCEECCCC
SLNQERYKGASILITRDNFGCGSSREHAPWALEDYGFRAIIAPSYADIFFNNCFKNGMLP
CCCHHHCCCEEEEEEECCCCCCCCCCCCCEEHHCCCEEEEECCCHHHHHHHHHHHCCCEE
VILKSEEVEELFHLVSTNVGAKVIVDLDKQTVTGPTGKIYYFEVDSFRKYCLYNGLDDIG
EEECCHHHHHHHHHHHCCCCCEEEEEECCCEECCCCCEEEEEEHHHHHHHHHHCCCCHHC
LTLKQESKIGEFEKKQKEVEPWLYAI
EEEECCCCCCHHHHHHHHCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA