Click here to switch to the map view.

The map label for this gene is leuC

Identifier: 45657687

GI number: 45657687

Start: 2216146

End: 2217543

Strand: Reverse

Name: leuC

Synonym: LIC11822

Alternate gene names: 45657687

Gene position: 2217543-2216146 (Counterclockwise)

Preceding gene: 45657688

Following gene: 45657686

Centisome position: 51.85

GC content: 42.49

Gene sequence:

>1398_bases
ATGAAGACAATGTTCGAAAAAATTTGGGAAGATCATCTAGTCGGAGAACTAGATGCTGGATCCTATCTAATCTATATAGA
TCGCCATCTCATTCATGAAGTTACAAGTCCTCAGGCGTTTGAAGGACTTAAACTTGCAGGCAGAAAGGTTCGTCGTCCTG
AAGCTACTTTTGCCACAATGGATCATAACGTTTCTACTAGAACACGTGATTTAAGTCTGGCCGATCCCGTTTCCGCAATT
CAAATGCAGACTTTAAAAAAGAACTGCGACGAAAATGGAATCCGCGTTTATGATTTTCAAAACCCTGACCAAGGAATCAT
TCACGTAATTGCTCCTGAAATGGGACTGACTCATCCTGGAATGACAATCGTATGCGGAGATTCTCATACTTCTACACACG
GTGCGTTTGGTGCGCTTGCTTTCGGGATCGGAACCAGCGAAGTAGAGCACGTTCTTGCGACTCAAACCTTAGTTCAAAAA
AGAGCAAAAACAATGGAGATTAGAGTCGATGGAAAACTTTCCGATAAGGTCACAGCAAAAGACATCATTCTTGCGATCAT
TGGAAAAATTGGAACCGCAGGTGCGACAGGTTATGTGATCGAATATAGAGGTTCTGCAATTCAAGCCCTCAGTATGGAAG
CTAGAATGACTATTTGTAATATGTCTATCGAAGCGGGAGCTAGAGCAGGTTTAATCGCACCAGATGAAACTACTTTTAAT
TATATTCAAGGAAAGGACTTTTCTCCAAAAGGAGTTGAATGGGATCTTGCGGTCAAAAAATGGAAACACTATGTAACGGA
CGAAGGTGCTAAATTTGATAGAACCGTAATTCTTCATGCAGATGAAATCGCTCCTATGGTAACTTGGGGAACTTCTCCCA
GTCAGGTTGTTTCGATAAAAGGAGTCGTTCCAGATCCAAAAGATGCAAATGATCCGGTGGAAAAAATTGGAATTGAGTCT
GCGCTTAAATATATGGATCTCAAATCGGGCCAGAAGATAGAAGACATTTCAATTAATAAAGTTTTTATCGGTTCCTGTAC
TAATTCTAGAATCGAAGATTTAAGAGCGGCCGCTGCTACCGTAAAAGGAAAAAAAGTTTCCTCTAAGGTTCAGGCGATTG
TGGTTCCTGGTTCAGGCAGAGTCAAACGTCAGGCGGAACAAGAAGGTCTGGATAAAATTTTTACCGCGGCCGGTTTTGAA
TGGAGAAATCCAGGCTGTTCTATGTGTCTTGCGATGAACGACGACGTATTAGAACCGGGAGATCGTTGTGCTTCTACTTC
TAACCGAAACTTTGAAGGTCGTCAAGGAAAAGGTGGAAGAACCCATCTAGTAGGACCGGAAATGGCCGCCGCCGCGGCTA
TCGAAGGCCATTTTGTGGATATTCGAAACTGGAAATAA

Upstream 100 bases:

>100_bases
AAGACCCGATTTCTCAACGATGGAATTTAGACGGATTCCATTTCGAGAAACCAATTATAAAAATTCCGAAAGAAATTCTT
TCGGGGAGTATTAGAATTCG

Downstream 100 bases:

>100_bases
AAAGGTAAATTAGAATATTATGAAACCCTTTACTATATTAAATGGAATTGCCGCCTTACTGGACAGACCCAACGTGGATA
CGGATCAGATCATTCCAAAA

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MKTMFEKIWEDHLVGELDAGSYLIYIDRHLIHEVTSPQAFEGLKLAGRKVRRPEATFATMDHNVSTRTRDLSLADPVSAI
QMQTLKKNCDENGIRVYDFQNPDQGIIHVIAPEMGLTHPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLVQK
RAKTMEIRVDGKLSDKVTAKDIILAIIGKIGTAGATGYVIEYRGSAIQALSMEARMTICNMSIEAGARAGLIAPDETTFN
YIQGKDFSPKGVEWDLAVKKWKHYVTDEGAKFDRTVILHADEIAPMVTWGTSPSQVVSIKGVVPDPKDANDPVEKIGIES
ALKYMDLKSGQKIEDISINKVFIGSCTNSRIEDLRAAAATVKGKKVSSKVQAIVVPGSGRVKRQAEQEGLDKIFTAAGFE
WRNPGCSMCLAMNDDVLEPGDRCASTSNRNFEGRQGKGGRTHLVGPEMAAAAAIEGHFVDIRNWK

Sequences:

>Translated_465_residues
MKTMFEKIWEDHLVGELDAGSYLIYIDRHLIHEVTSPQAFEGLKLAGRKVRRPEATFATMDHNVSTRTRDLSLADPVSAI
QMQTLKKNCDENGIRVYDFQNPDQGIIHVIAPEMGLTHPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLVQK
RAKTMEIRVDGKLSDKVTAKDIILAIIGKIGTAGATGYVIEYRGSAIQALSMEARMTICNMSIEAGARAGLIAPDETTFN
YIQGKDFSPKGVEWDLAVKKWKHYVTDEGAKFDRTVILHADEIAPMVTWGTSPSQVVSIKGVVPDPKDANDPVEKIGIES
ALKYMDLKSGQKIEDISINKVFIGSCTNSRIEDLRAAAATVKGKKVSSKVQAIVVPGSGRVKRQAEQEGLDKIFTAAGFE
WRNPGCSMCLAMNDDVLEPGDRCASTSNRNFEGRQGKGGRTHLVGPEMAAAAAIEGHFVDIRNWK
>Mature_465_residues
MKTMFEKIWEDHLVGELDAGSYLIYIDRHLIHEVTSPQAFEGLKLAGRKVRRPEATFATMDHNVSTRTRDLSLADPVSAI
QMQTLKKNCDENGIRVYDFQNPDQGIIHVIAPEMGLTHPGMTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLVQK
RAKTMEIRVDGKLSDKVTAKDIILAIIGKIGTAGATGYVIEYRGSAIQALSMEARMTICNMSIEAGARAGLIAPDETTFN
YIQGKDFSPKGVEWDLAVKKWKHYVTDEGAKFDRTVILHADEIAPMVTWGTSPSQVVSIKGVVPDPKDANDPVEKIGIES
ALKYMDLKSGQKIEDISINKVFIGSCTNSRIEDLRAAAATVKGKKVSSKVQAIVVPGSGRVKRQAEQEGLDKIFTAAGFE
WRNPGCSMCLAMNDDVLEPGDRCASTSNRNFEGRQGKGGRTHLVGPEMAAAAAIEGHFVDIRNWK

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=370, Percent_Identity=28.6486486486486, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI8659555, Length=406, Percent_Identity=26.3546798029557, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI41352693, Length=380, Percent_Identity=26.5789473684211, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=66.3793103448276, Blast_Score=639, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=359, Percent_Identity=25.6267409470752, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25149337, Length=369, Percent_Identity=30.6233062330623, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI32564738, Length=369, Percent_Identity=30.6233062330623, Blast_Score=131, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI25149342, Length=308, Percent_Identity=29.8701298701299, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17568399, Length=402, Percent_Identity=26.865671641791, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=59.6602972399151, Blast_Score=578, Evalue=1e-166,
Organism=Saccharomyces cerevisiae, GI6320440, Length=437, Percent_Identity=25.858123569794, Blast_Score=140, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6323335, Length=369, Percent_Identity=28.9972899728997, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6322261, Length=368, Percent_Identity=27.1739130434783, Blast_Score=129, Evalue=8e-31,
Organism=Drosophila melanogaster, GI28571643, Length=474, Percent_Identity=28.0590717299578, Blast_Score=128, Evalue=7e-30,
Organism=Drosophila melanogaster, GI281365315, Length=475, Percent_Identity=27.5789473684211, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17864292, Length=475, Percent_Identity=27.5789473684211, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI161076999, Length=399, Percent_Identity=28.3208020050125, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24645686, Length=371, Percent_Identity=28.0323450134771, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17137564, Length=372, Percent_Identity=27.1505376344086, Blast_Score=102, Evalue=5e-22,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_LEPIC (Q72RC4)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_001773.1
- ProteinModelPortal:   Q72RC4
- SMR:   Q72RC4
- GeneID:   2769840
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC11822
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- ProtClustDB:   PRK05478
- BioCyc:   LINT267671:LIC_11822-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 50642; Mature: 50642

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTMFEKIWEDHLVGELDAGSYLIYIDRHLIHEVTSPQAFEGLKLAGRKVRRPEATFATM
CCHHHHHHHHHHCCEEECCCCEEEEEEHHHHHHCCCCHHHCCHHHCCCCCCCCCCEEEEE
DHNVSTRTRDLSLADPVSAIQMQTLKKNCDENGIRVYDFQNPDQGIIHVIAPEMGLTHPG
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCC
MTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLVQKRAKTMEIRVDGKLSDKVTAK
CEEEECCCCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHH
DIILAIIGKIGTAGATGYVIEYRGSAIQALSMEARMTICNMSIEAGARAGLIAPDETTFN
HHHHHHHHHCCCCCCCEEEEEECCCEEEEEEHHEEEEEEEEEHHCCCCCCCCCCCCCCEE
YIQGKDFSPKGVEWDLAVKKWKHYVTDEGAKFDRTVILHADEIAPMVTWGTSPSQVVSIK
EEECCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCEEEEEE
GVVPDPKDANDPVEKIGIESALKYMDLKSGQKIEDISINKVFIGSCTNSRIEDLRAAAAT
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECEEEEEEEEEECCCCHHHHHHHHHHHH
VKGKKVSSKVQAIVVPGSGRVKRQAEQEGLDKIFTAAGFEWRNPGCSMCLAMNDDVLEPG
HCCCEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCC
DRCASTSNRNFEGRQGKGGRTHLVGPEMAAAAAIEGHFVDIRNWK
HHHCCCCCCCCCCCCCCCCCEEECCCHHHHHHEECCEEEEECCCC
>Mature Secondary Structure
MKTMFEKIWEDHLVGELDAGSYLIYIDRHLIHEVTSPQAFEGLKLAGRKVRRPEATFATM
CCHHHHHHHHHHCCEEECCCCEEEEEEHHHHHHCCCCHHHCCHHHCCCCCCCCCCEEEEE
DHNVSTRTRDLSLADPVSAIQMQTLKKNCDENGIRVYDFQNPDQGIIHVIAPEMGLTHPG
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEEEECCCCCCCCC
MTIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLVQKRAKTMEIRVDGKLSDKVTAK
CEEEECCCCCCCCCCHHHHEECCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCHHH
DIILAIIGKIGTAGATGYVIEYRGSAIQALSMEARMTICNMSIEAGARAGLIAPDETTFN
HHHHHHHHHCCCCCCCEEEEEECCCEEEEEEHHEEEEEEEEEHHCCCCCCCCCCCCCCEE
YIQGKDFSPKGVEWDLAVKKWKHYVTDEGAKFDRTVILHADEIAPMVTWGTSPSQVVSIK
EEECCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECCCCCEEEEEE
GVVPDPKDANDPVEKIGIESALKYMDLKSGQKIEDISINKVFIGSCTNSRIEDLRAAAAT
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEECEEEEEEEEEECCCCHHHHHHHHHHHH
VKGKKVSSKVQAIVVPGSGRVKRQAEQEGLDKIFTAAGFEWRNPGCSMCLAMNDDVLEPG
HCCCEECCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCC
DRCASTSNRNFEGRQGKGGRTHLVGPEMAAAAAIEGHFVDIRNWK
HHHCCCCCCCCCCCCCCCCCEEECCCHHHHHHEECCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA