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The map label for this gene is tgt

Identifier: 45656892

GI number: 45656892

Start: 1223051

End: 1224175

Strand: Reverse

Name: tgt

Synonym: LIC11005

Alternate gene names: 45656892

Gene position: 1224175-1223051 (Counterclockwise)

Preceding gene: 45656893

Following gene: 45656891

Centisome position: 28.62

GC content: 39.56

Gene sequence:

>1125_bases
ATGATTTTTCAAACAACTTCGGAAGACACTCTTACTAAAGCCAGAACCGGAATTTTAAATTTAAACGGAATAGAATTAAA
AACCCCTGTATTTATGCCGGTGGGTACGAGAGGAGTCGTTAAAACCCTTTCAGCAGACGACCTAGAAGAACTTGAGTATT
CTCTAATATTAGGAAATACGTATCACCTCTATCTCAGACCCGGGACTTCCGTATTGGATCGTTTTGGAGGACTCAAAAAA
TTCTCAACCTGGAAAAAAGCACTTCTAACGGATAGCGGAGGATATCAAGTATTCAGTTTAAATTCACTTTTTAAATACGA
ACAGGATGGCGTTCGTTTTCAATCTCATATAGACGGGAGTAGACATTATTTTACTCCCAACTCCGTTATAGACATACAAA
GAAGTATTGGTTCCGATATTATGATGGTTTTAGACGACTGTGCGCCTTTCGATTCTGGCCCTGAAAGGCTCAAACAATCC
TTAGATAGAACCCATCGGTGGGCAGAAATGTCGGTACAATATTGGGAAAAGAATAAGAATTCCCAGCATCTTTTTGGAAT
TTTTCAGGGAGGAATCGATCTTGATTTTCGGCTGGAAAGTTTAAATACGATCACCTCACTACCGTTTGACGGAATTGCAA
TCGGAGGGCTTTCGGTCGGAGAACCTCGCAAAGATTTCATTCGAATTTTGGACGGTATTTCGGCCCACACAGATCGAAAT
CGTCCTCTTTATTTGATGGGAGTCGGTACGGTACCGGATATTTTGGACGGAGTGAAAAACGGAGTCGATATGTTTGACTG
TGTACTTCCGACCAGAAACGCAAGAAACGGCCAAGTATTTACAACTTTAGGAAAAATCAATTTAAGAAACGAAAAATGGA
AAAGCTCCGATACACCAATGGACCCGAATTGCACGTGTAAGGTGTGTAAAAGATATAGCATAGGGTACATCAGACACCTA
CATCATGTAGGAGAGATCACTGCATTCTCACTTTCAACGTACCATAATTTACATTTTATGAAAAATTTTCTGACAGAGAT
TCAAAACTCCATTCAAAAAGGAGAATTTTTAGAAATCTATGCTAAGTGGAAAAATTTGTACGAAAAGCCCGAATTTTCCG
GTTGA

Upstream 100 bases:

>100_bases
TAGAACAACTTCAAGAACAAGCAGCAAGAGAAAACGGTTTCAAAATCACAGGTCATAGCTTGAACATTTACGGAACTTGC
AACGAACATTCTTCTAGTAA

Downstream 100 bases:

>100_bases
CTATTTAAAGAATCGACTTTCCTTTAGTAGAGCTGGCTGGAAAGAAATAGAAAGGAGTTGCTGATTATTTATGGAAATAA
CCAGAAGAGAAAGCGGGAAC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MIFQTTSEDTLTKARTGILNLNGIELKTPVFMPVGTRGVVKTLSADDLEELEYSLILGNTYHLYLRPGTSVLDRFGGLKK
FSTWKKALLTDSGGYQVFSLNSLFKYEQDGVRFQSHIDGSRHYFTPNSVIDIQRSIGSDIMMVLDDCAPFDSGPERLKQS
LDRTHRWAEMSVQYWEKNKNSQHLFGIFQGGIDLDFRLESLNTITSLPFDGIAIGGLSVGEPRKDFIRILDGISAHTDRN
RPLYLMGVGTVPDILDGVKNGVDMFDCVLPTRNARNGQVFTTLGKINLRNEKWKSSDTPMDPNCTCKVCKRYSIGYIRHL
HHVGEITAFSLSTYHNLHFMKNFLTEIQNSIQKGEFLEIYAKWKNLYEKPEFSG

Sequences:

>Translated_374_residues
MIFQTTSEDTLTKARTGILNLNGIELKTPVFMPVGTRGVVKTLSADDLEELEYSLILGNTYHLYLRPGTSVLDRFGGLKK
FSTWKKALLTDSGGYQVFSLNSLFKYEQDGVRFQSHIDGSRHYFTPNSVIDIQRSIGSDIMMVLDDCAPFDSGPERLKQS
LDRTHRWAEMSVQYWEKNKNSQHLFGIFQGGIDLDFRLESLNTITSLPFDGIAIGGLSVGEPRKDFIRILDGISAHTDRN
RPLYLMGVGTVPDILDGVKNGVDMFDCVLPTRNARNGQVFTTLGKINLRNEKWKSSDTPMDPNCTCKVCKRYSIGYIRHL
HHVGEITAFSLSTYHNLHFMKNFLTEIQNSIQKGEFLEIYAKWKNLYEKPEFSG
>Mature_374_residues
MIFQTTSEDTLTKARTGILNLNGIELKTPVFMPVGTRGVVKTLSADDLEELEYSLILGNTYHLYLRPGTSVLDRFGGLKK
FSTWKKALLTDSGGYQVFSLNSLFKYEQDGVRFQSHIDGSRHYFTPNSVIDIQRSIGSDIMMVLDDCAPFDSGPERLKQS
LDRTHRWAEMSVQYWEKNKNSQHLFGIFQGGIDLDFRLESLNTITSLPFDGIAIGGLSVGEPRKDFIRILDGISAHTDRN
RPLYLMGVGTVPDILDGVKNGVDMFDCVLPTRNARNGQVFTTLGKINLRNEKWKSSDTPMDPNCTCKVCKRYSIGYIRHL
HHVGEITAFSLSTYHNLHFMKNFLTEIQNSIQKGEFLEIYAKWKNLYEKPEFSG

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=355, Percent_Identity=37.4647887323944, Blast_Score=262, Evalue=4e-70,
Organism=Escherichia coli, GI1786607, Length=356, Percent_Identity=41.2921348314607, Blast_Score=310, Evalue=9e-86,
Organism=Caenorhabditis elegans, GI17544680, Length=341, Percent_Identity=36.0703812316716, Blast_Score=221, Evalue=6e-58,
Organism=Drosophila melanogaster, GI19920510, Length=371, Percent_Identity=39.0835579514825, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI21355311, Length=222, Percent_Identity=22.972972972973, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42434; Mature: 42434

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFQTTSEDTLTKARTGILNLNGIELKTPVFMPVGTRGVVKTLSADDLEELEYSLILGNT
CCCCCCCCHHHHHHHHCEEECCCEEEECCEEEECCCHHHHHHCCCCCHHHCEEEEEECCE
YHLYLRPGTSVLDRFGGLKKFSTWKKALLTDSGGYQVFSLNSLFKYEQDGVRFQSHIDGS
EEEEECCCHHHHHHHCCCHHHHHHHHHHEECCCCEEEEEEHHHHHHCCCCCEEHHCCCCC
RHYFTPNSVIDIQRSIGSDIMMVLDDCAPFDSGPERLKQSLDRTHRWAEMSVQYWEKNKN
CEEECCCCHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
SQHLFGIFQGGIDLDFRLESLNTITSLPFDGIAIGGLSVGEPRKDFIRILDGISAHTDRN
CCEEEEEEECCCCEEEEECCHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHCHHCCCCCC
RPLYLMGVGTVPDILDGVKNGVDMFDCVLPTRNARNGQVFTTLGKINLRNEKWKSSDTPM
CCEEEEECCCHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCC
DPNCTCKVCKRYSIGYIRHLHHVGEITAFSLSTYHNLHFMKNFLTEIQNSIQKGEFLEIY
CCCCHHHHHHHCCHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AKWKNLYEKPEFSG
HHHHHHHCCCCCCC
>Mature Secondary Structure
MIFQTTSEDTLTKARTGILNLNGIELKTPVFMPVGTRGVVKTLSADDLEELEYSLILGNT
CCCCCCCCHHHHHHHHCEEECCCEEEECCEEEECCCHHHHHHCCCCCHHHCEEEEEECCE
YHLYLRPGTSVLDRFGGLKKFSTWKKALLTDSGGYQVFSLNSLFKYEQDGVRFQSHIDGS
EEEEECCCHHHHHHHCCCHHHHHHHHHHEECCCCEEEEEEHHHHHHCCCCCEEHHCCCCC
RHYFTPNSVIDIQRSIGSDIMMVLDDCAPFDSGPERLKQSLDRTHRWAEMSVQYWEKNKN
CEEECCCCHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
SQHLFGIFQGGIDLDFRLESLNTITSLPFDGIAIGGLSVGEPRKDFIRILDGISAHTDRN
CCEEEEEEECCCCEEEEECCHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHCHHCCCCCC
RPLYLMGVGTVPDILDGVKNGVDMFDCVLPTRNARNGQVFTTLGKINLRNEKWKSSDTPM
CCEEEEECCCHHHHHHHHHCCCHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCC
DPNCTCKVCKRYSIGYIRHLHHVGEITAFSLSTYHNLHFMKNFLTEIQNSIQKGEFLEIY
CCCCHHHHHHHCCHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
AKWKNLYEKPEFSG
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA