BacMap

Definition	Leptospira interrogans serovar Copenhageni str. Fiocruz L1-130 chromosome chromosome I, complete sequence.
Accession	NC_005823
Length	4,277,185

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The map label for this gene is dkgA

Identifier: 45656837

GI number: 45656837

Start: 1143623

End: 1144459

Strand: Reverse

Name: dkgA

Synonym: LIC10949

Alternate gene names: 45656837

Gene position: 1144459-1143623 (Counterclockwise)

Preceding gene: 45656838

Following gene: 45656836

Centisome position: 26.76

GC content: 34.89

Gene sequence:

>837_bases
ATGAACAACAAAGAGAACCCACTTAAGCAAACGATAATGCTCAACAATGGAATTTCTATGCCGATTTTAGGGCTTGGAGT
TTGGAAAACCAAATCCGGCAAAGAATGCAAAGAAGCCGTCTTAAACGCCTTGGAAGCGGGTTATCGCCATATCGATACGG
CCAGAATTTATGATAACGAAGTAGATGTAGGTCAAGCAATTCGAGAAAGTGGAATTCCAAGAAAAGAAATTTTTATAACT
ACAAAACTTTGGAATGCAGATCAAGGGTCGGATAAAACCAGAAAGGCTCTCGAAAATAGTCTTGATAGATTAGGAATCGA
TTTCGTGGATCTTTATCTCATCCATTTTCCAGTGACTTCTAAAAGAATGGATTCTTGGAAAGAATTGGAAAAATTGTACC
ATGATAAACTCTGTAAAGCGATCGGAGTCAGTAACTATACAATTATCCATCTTACAGAATTACTAAAAAATTCTCAAATT
ACTCCTGCAGTCAATCAGGTGGAATTTCATCCGTTTCTAAATCAAATTCATTTATTAGAATATTGTAAAAAACATAAAAT
CCAACTCGAAGCTTATAGTCCACTTGCACATGGTCAAAAAATAGAAGACCCTACGATCGCAAAAATTGCACAAAAATATG
ATAAAACTCCGGCTCAGATTTTGATTCGTTGGGCCATAGAACAAAAAATCGTAGTCATTCCTAAATCTATAAAAAAGGAA
AGAATTATCGAAAATTCTAAAGTTTTTGATTTTGCAATTTCGGAAGAAGATATGAAAATTCTTAATTCATTGGATGAAGA
TTTCAGAACCTGTTGGGACCCATCCGAAGTCGTTTAA

Upstream 100 bases:

>100_bases
ACTTGATTTGATAGAGGTGAGTAATAGAAATTCATAATTTCATTCTTAAGTTGTTGCGTTTTATTCCTTTTACAATCAAA
AATCAAATCAGGAGCCAAAG

Downstream 100 bases:

>100_bases
AACTTGTCCTACCGATACCTATAAAATTGGGTTAACGCAAAGCCTTGTTTCAATAGAAAATATTGATACTTTGTTTTACA
GTTATTGAGTAAAATCTTAA

Product: aldo/keto reductase

Products: NA

Alternate protein names: GR; Methylglyoxal reductase [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MNNKENPLKQTIMLNNGISMPILGLGVWKTKSGKECKEAVLNALEAGYRHIDTARIYDNEVDVGQAIRESGIPRKEIFIT
TKLWNADQGSDKTRKALENSLDRLGIDFVDLYLIHFPVTSKRMDSWKELEKLYHDKLCKAIGVSNYTIIHLTELLKNSQI
TPAVNQVEFHPFLNQIHLLEYCKKHKIQLEAYSPLAHGQKIEDPTIAKIAQKYDKTPAQILIRWAIEQKIVVIPKSIKKE
RIIENSKVFDFAISEEDMKILNSLDEDFRTCWDPSEVV

Sequences:

>Translated_278_residues
MNNKENPLKQTIMLNNGISMPILGLGVWKTKSGKECKEAVLNALEAGYRHIDTARIYDNEVDVGQAIRESGIPRKEIFIT
TKLWNADQGSDKTRKALENSLDRLGIDFVDLYLIHFPVTSKRMDSWKELEKLYHDKLCKAIGVSNYTIIHLTELLKNSQI
TPAVNQVEFHPFLNQIHLLEYCKKHKIQLEAYSPLAHGQKIEDPTIAKIAQKYDKTPAQILIRWAIEQKIVVIPKSIKKE
RIIENSKVFDFAISEEDMKILNSLDEDFRTCWDPSEVV
>Mature_278_residues
MNNKENPLKQTIMLNNGISMPILGLGVWKTKSGKECKEAVLNALEAGYRHIDTARIYDNEVDVGQAIRESGIPRKEIFIT
TKLWNADQGSDKTRKALENSLDRLGIDFVDLYLIHFPVTSKRMDSWKELEKLYHDKLCKAIGVSNYTIIHLTELLKNSQI
TPAVNQVEFHPFLNQIHLLEYCKKHKIQLEAYSPLAHGQKIEDPTIAKIAQKYDKTPAQILIRWAIEQKIVVIPKSIKKE
RIIENSKVFDFAISEEDMKILNSLDEDFRTCWDPSEVV

Specific function: Reduces glyoxal and methylglyoxal. Is not involved in the vitamin B6 biosynthesis [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=293, Percent_Identity=38.2252559726962, Blast_Score=227, Evalue=8e-60,
Organism=Homo sapiens, GI5174391, Length=293, Percent_Identity=38.2252559726962, Blast_Score=227, Evalue=8e-60,
Organism=Homo sapiens, GI223468663, Length=296, Percent_Identity=39.1891891891892, Blast_Score=211, Evalue=5e-55,
Organism=Homo sapiens, GI4502049, Length=300, Percent_Identity=40.3333333333333, Blast_Score=201, Evalue=5e-52,
Organism=Homo sapiens, GI310109922, Length=280, Percent_Identity=39.6428571428571, Blast_Score=199, Evalue=3e-51,
Organism=Homo sapiens, GI310109920, Length=300, Percent_Identity=37.3333333333333, Blast_Score=195, Evalue=4e-50,
Organism=Homo sapiens, GI5453543, Length=299, Percent_Identity=37.123745819398, Blast_Score=195, Evalue=5e-50,
Organism=Homo sapiens, GI45446745, Length=300, Percent_Identity=37.3333333333333, Blast_Score=194, Evalue=6e-50,
Organism=Homo sapiens, GI4503285, Length=300, Percent_Identity=37.3333333333333, Blast_Score=194, Evalue=6e-50,
Organism=Homo sapiens, GI24497585, Length=303, Percent_Identity=36.3036303630363, Blast_Score=191, Evalue=5e-49,
Organism=Homo sapiens, GI24497583, Length=298, Percent_Identity=36.241610738255, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI5174695, Length=298, Percent_Identity=35.9060402684564, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI93277124, Length=304, Percent_Identity=34.5394736842105, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI291291012, Length=272, Percent_Identity=36.7647058823529, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI300116271, Length=281, Percent_Identity=35.5871886120996, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI300116273, Length=277, Percent_Identity=35.7400722021661, Blast_Score=165, Evalue=4e-41,
Organism=Homo sapiens, GI310109926, Length=131, Percent_Identity=44.2748091603053, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI207028673, Length=131, Percent_Identity=44.2748091603053, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI310109924, Length=155, Percent_Identity=36.1290322580645, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI27436964, Length=222, Percent_Identity=28.3783783783784, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI87082198, Length=270, Percent_Identity=44.0740740740741, Blast_Score=228, Evalue=4e-61,
Organism=Escherichia coli, GI1786400, Length=256, Percent_Identity=38.28125, Blast_Score=181, Evalue=4e-47,
Organism=Escherichia coli, GI1788081, Length=272, Percent_Identity=29.0441176470588, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788070, Length=327, Percent_Identity=22.0183486238532, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1787674, Length=270, Percent_Identity=24.4444444444444, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71998625, Length=277, Percent_Identity=42.5992779783394, Blast_Score=226, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17552492, Length=278, Percent_Identity=35.9712230215827, Blast_Score=194, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17566692, Length=285, Percent_Identity=38.9473684210526, Blast_Score=190, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17564128, Length=298, Percent_Identity=36.9127516778523, Blast_Score=189, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17550248, Length=285, Percent_Identity=37.5438596491228, Blast_Score=188, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17537075, Length=301, Percent_Identity=35.2159468438538, Blast_Score=187, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17537077, Length=295, Percent_Identity=34.5762711864407, Blast_Score=185, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17561298, Length=270, Percent_Identity=35.1851851851852, Blast_Score=184, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17562292, Length=282, Percent_Identity=32.6241134751773, Blast_Score=161, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17538386, Length=272, Percent_Identity=33.4558823529412, Blast_Score=154, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17537079, Length=303, Percent_Identity=28.7128712871287, Blast_Score=151, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17561300, Length=275, Percent_Identity=32.7272727272727, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320576, Length=288, Percent_Identity=39.9305555555556, Blast_Score=211, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6324694, Length=304, Percent_Identity=39.4736842105263, Blast_Score=209, Evalue=3e-55,
Organism=Saccharomyces cerevisiae, GI6321896, Length=312, Percent_Identity=34.9358974358974, Blast_Score=186, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6322556, Length=275, Percent_Identity=34.1818181818182, Blast_Score=182, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6319625, Length=295, Percent_Identity=37.2881355932203, Blast_Score=173, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6320079, Length=295, Percent_Identity=33.8983050847458, Blast_Score=155, Evalue=6e-39,
Organism=Drosophila melanogaster, GI24663317, Length=289, Percent_Identity=38.7543252595156, Blast_Score=204, Evalue=5e-53,
Organism=Drosophila melanogaster, GI24662789, Length=295, Percent_Identity=36.271186440678, Blast_Score=204, Evalue=5e-53,
Organism=Drosophila melanogaster, GI20129731, Length=291, Percent_Identity=38.8316151202749, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24657054, Length=282, Percent_Identity=40.7801418439716, Blast_Score=200, Evalue=9e-52,
Organism=Drosophila melanogaster, GI45553081, Length=291, Percent_Identity=37.4570446735395, Blast_Score=194, Evalue=4e-50,
Organism=Drosophila melanogaster, GI24644950, Length=302, Percent_Identity=36.4238410596026, Blast_Score=194, Evalue=7e-50,
Organism=Drosophila melanogaster, GI21356425, Length=288, Percent_Identity=38.1944444444444, Blast_Score=191, Evalue=6e-49,
Organism=Drosophila melanogaster, GI221378297, Length=322, Percent_Identity=34.1614906832298, Blast_Score=186, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24662781, Length=292, Percent_Identity=39.7260273972603, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24662785, Length=300, Percent_Identity=38, Blast_Score=171, Evalue=5e-43,
Organism=Drosophila melanogaster, GI281366140, Length=252, Percent_Identity=35.3174603174603, Blast_Score=157, Evalue=6e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.283 [H]

Molecular weight: Translated: 31973; Mature: 31973

Theoretical pI: Translated: 7.60; Mature: 7.60

Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKENPLKQTIMLNNGISMPILGLGVWKTKSGKECKEAVLNALEAGYRHIDTARIYDNE
CCCCCCHHHHHEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCC
VDVGQAIRESGIPRKEIFITTKLWNADQGSDKTRKALENSLDRLGIDFVDLYLIHFPVTS
CHHHHHHHHHCCCCHHEEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCH
KRMDSWKELEKLYHDKLCKAIGVSNYTIIHLTELLKNSQITPAVNQVEFHPFLNQIHLLE
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHEEHHHHHHHHHHHH
YCKKHKIQLEAYSPLAHGQKIEDPTIAKIAQKYDKTPAQILIRWAIEQKIVVIPKSIKKE
HHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCEEEEECCHHHHH
RIIENSKVFDFAISEEDMKILNSLDEDFRTCWDPSEVV
HHHCCCCEEEEEECHHHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure
MNNKENPLKQTIMLNNGISMPILGLGVWKTKSGKECKEAVLNALEAGYRHIDTARIYDNE
CCCCCCHHHHHEEECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCC
VDVGQAIRESGIPRKEIFITTKLWNADQGSDKTRKALENSLDRLGIDFVDLYLIHFPVTS
CHHHHHHHHHCCCCHHEEEEEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCH
KRMDSWKELEKLYHDKLCKAIGVSNYTIIHLTELLKNSQITPAVNQVEFHPFLNQIHLLE
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHEEHHHHHHHHHHHH
YCKKHKIQLEAYSPLAHGQKIEDPTIAKIAQKYDKTPAQILIRWAIEQKIVVIPKSIKKE
HHHHCCEEEEEECCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCEEEEECCHHHHH
RIIENSKVFDFAISEEDMKILNSLDEDFRTCWDPSEVV
HHHCCCCEEEEEECHHHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]