Click here to switch to the map view.

The map label for this gene is hisH

Identifier: 45656023

GI number: 45656023

Start: 130616

End: 131248

Strand: Direct

Name: hisH

Synonym: LIC10113

Alternate gene names: 45656023

Gene position: 130616-131248 (Clockwise)

Preceding gene: 45656022

Following gene: 45656024

Centisome position: 3.05

GC content: 33.65

Gene sequence:

>633_bases
ATGATCGCCATTCTTGATTATGGAATGGGAAATATTCATTCTTGTTTAAAAGCCGTATCTCTTTATACTAAGGATTTTGT
TTTTACAAAAGATCATTCAACGATTGAAAATTCAAAAGCTCTAATATTACCAGGTGACGGACATTTTGATAAAGCGATGG
AGAACTTGAATTCAACTGGACTTCGGAAAACGATTGATAAACACGTAACTTCCGGAAAACCTCTTTTCGGAATTTGTATC
GGCTTTCAAATTCTTTTTGAATCTTCAGAAGAGATTGCTCAAGGTTCAAAAAAAGAGCAGATCGAAGGTTTAGGATATAT
CAAAGGTAAAATCAAAAAATTTCACGGAAAGGATTTTAAAGTTCCGCATATTGGTTGGAATCGCCTTCAAATTCGTAGAA
AAGATAAAAGTGTTCTTTTAAAAGGAATTGGAGATCAATCTTTTTTTTACTTCATTCATTCTTATAGACCTACTGACGCT
GAAGGAAATGCGATCACCGGACTTTGTGATTACTACCAGGAAAAATTTCCAGCCGTTGTAGAGAAAAATAATATTTTCGG
AACACAGTTCCATCCTGAAAAATCACATACCCACGGACTTAAACTTTTGGAGAATTTTATTCGTTTTATATGA

Upstream 100 bases:

>100_bases
ACGAATCTATTTTTAAAGCTTTAGGTAAAGCACTCAGAATGGCGATTGCACAGGACTCTGCAGCCGCAGGTGCAATTCCT
TCCACTAAAGGAGTCTTAGA

Downstream 100 bases:

>100_bases
TTGTAATTCCTGCTATAGATCTATTCGATAATTGTGCAGTTCGTTTATTTAAGGGAAACTATGAAGAAAAGAAAATCTAT
TCTTCCGAACCTTGGAAACT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MIAILDYGMGNIHSCLKAVSLYTKDFVFTKDHSTIENSKALILPGDGHFDKAMENLNSTGLRKTIDKHVTSGKPLFGICI
GFQILFESSEEIAQGSKKEQIEGLGYIKGKIKKFHGKDFKVPHIGWNRLQIRRKDKSVLLKGIGDQSFFYFIHSYRPTDA
EGNAITGLCDYYQEKFPAVVEKNNIFGTQFHPEKSHTHGLKLLENFIRFI

Sequences:

>Translated_210_residues
MIAILDYGMGNIHSCLKAVSLYTKDFVFTKDHSTIENSKALILPGDGHFDKAMENLNSTGLRKTIDKHVTSGKPLFGICI
GFQILFESSEEIAQGSKKEQIEGLGYIKGKIKKFHGKDFKVPHIGWNRLQIRRKDKSVLLKGIGDQSFFYFIHSYRPTDA
EGNAITGLCDYYQEKFPAVVEKNNIFGTQFHPEKSHTHGLKLLENFIRFI
>Mature_210_residues
MIAILDYGMGNIHSCLKAVSLYTKDFVFTKDHSTIENSKALILPGDGHFDKAMENLNSTGLRKTIDKHVTSGKPLFGICI
GFQILFESSEEIAQGSKKEQIEGLGYIKGKIKKFHGKDFKVPHIGWNRLQIRRKDKSVLLKGIGDQSFFYFIHSYRPTDA
EGNAITGLCDYYQEKFPAVVEKNNIFGTQFHPEKSHTHGLKLLENFIRFI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=209, Percent_Identity=33.0143540669856, Blast_Score=117, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6319725, Length=219, Percent_Identity=34.2465753424658, Blast_Score=118, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS51_LEPIN (P59118)

Other databases:

- EMBL:   AE010300
- RefSeq:   NP_710306.1
- ProteinModelPortal:   P59118
- SMR:   P59118
- GeneID:   1149468
- GenomeReviews:   AE010300_GR
- HOGENOM:   HBG292341
- OMA:   FRGIENG
- ProtClustDB:   PRK14004
- BioCyc:   LINT-130-01:LINT-130-01-000109-MONOMER
- BioCyc:   LINT189518:LA0125-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 23830; Mature: 23830

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 79-79 ACT_SITE 191-191 ACT_SITE 193-193

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAILDYGMGNIHSCLKAVSLYTKDFVFTKDHSTIENSKALILPGDGHFDKAMENLNSTG
CEEEEECCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEECCCCCHHHHHHHHCCCH
LRKTIDKHVTSGKPLFGICIGFQILFESSEEIAQGSKKEQIEGLGYIKGKIKKFHGKDFK
HHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHCCCCHHHHHCCHHHHHHHHHHCCCCCC
VPHIGWNRLQIRRKDKSVLLKGIGDQSFFYFIHSYRPTDAEGNAITGLCDYYQEKFPAVV
CCCCCCCCEEEEECCHHHHHHCCCCCEEEEEEEECCCCCCCCCEEHHHHHHHHHHCCCEE
EKNNIFGTQFHPEKSHTHGLKLLENFIRFI
ECCCEECCCCCCCCCCHHHHHHHHHHHHHC
>Mature Secondary Structure
MIAILDYGMGNIHSCLKAVSLYTKDFVFTKDHSTIENSKALILPGDGHFDKAMENLNSTG
CEEEEECCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCEEEECCCCCHHHHHHHHCCCH
LRKTIDKHVTSGKPLFGICIGFQILFESSEEIAQGSKKEQIEGLGYIKGKIKKFHGKDFK
HHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHCCCCHHHHHCCHHHHHHHHHHCCCCCC
VPHIGWNRLQIRRKDKSVLLKGIGDQSFFYFIHSYRPTDAEGNAITGLCDYYQEKFPAVV
CCCCCCCCEEEEECCHHHHHHCCCCCEEEEEEEECCCCCCCCCEEHHHHHHHHHHCCCEE
EKNNIFGTQFHPEKSHTHGLKLLENFIRFI
ECCCEECCCCCCCCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12712204