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The map label for this gene is nifK

Identifier: 45358420

GI number: 45358420

Start: 847128

End: 848516

Strand: Direct

Name: nifK

Synonym: MMP0857

Alternate gene names: 45358420

Gene position: 847128-848516 (Clockwise)

Preceding gene: 45358419

Following gene: 45358421

Centisome position: 51.0

GC content: 38.16

Gene sequence:

>1389_bases
ATGAGTGAGTTAAACGTAATCAAAAAAGAAAGAACCGCGGTAATTAACCCAATCGTAACATGCCAACCTTTAGGTGCAAT
GTACGCAGTATCTGGAATTGAAAGAGGAATGCCTTTAGTTCACGGATCACAAGGATGTTCAACATTCGTAAGATATGGAT
TTGCAAGACACTTTAGAGAACCTGCAGATATTGCAGTAACCTCGCTTCACGAGGATGCAGCAGTATTCGGTGGAAGAAAA
AACCTGATTTCAGGTCTCGGAAACTTAGCTGCAAGGTTTAAACCTGATGTAATGGGTGTTGTAACAACATGTTCCAGTGA
AATCATCGGAGATGACGTTGCTGGTTTCATTAAAACAGCTAAAGTAGAAATTGCAAAGAAAATGGGTGAAGAAGCCGCTA
ACAAAATCAAAATTGTTCAGATTAATACCCCAAGTTTCGTTGAACACCAGTTCAAAGGATACGACAATGCAATAAAAGCA
ATTGTTGACACATTAGCTGAACCAAAAGACGAAGAAAATGGAAAATTAAATATCATTCCAGGAATTGTAAACCCTGGAGA
CATCAGAGAAATCAAACATATGCTTTCACTCATGGGAGTTGAAGGTATTTTACTTACAGATACCTCTGATCCATTTGATT
CACCACTCAGACCATCAAAAGCAGACAAAAATCCATACTACCAAAAAGGTGGAACACCTCTTGCAGATCTTCAAGACTGT
GCAAACTCACTTGGTACAATTTCACTTGCTAACTATGCAAACAGTGCTCCTGCATCACTCGAGAAAAAATACAACATGCC
TTCAAAAGTGAGTGAGGCACCAATTGGTATTCAAAATACTGACAGTTTCATAAGAACTGTTAAAAAATTCACTGGAAACG
ACGTTACTGATGAAATACTTGACGAAAGAGGTATTGTGATTGACGCAATGGCTGACGTTGCATCAAGATACTTATTCGGA
AGAAAAGTAGCAATCTACGGTGACCCATCAATAACTGTAGGAATGGCAAGATTTGTAGCAGAACTTGGAATGATTCCTAA
AGTGGTATGTACTGGAGTTAAAAACGAATACTTCGTTAATGACTTGAAAAAAGTAGCTAAAGAATCAGATGAAGACATCG
ATGCACTCTTCGGACAAGACTTAAGAGCATTAGATGTTTACTTAAAAGAAAACCCAGTCGATTTAATGATTGGTAGCTCA
GATGGTAGATTGATGGCAAAAGACCTCGGAATACCACTCTACAGAGTAGGATACCCAGTATACGATAGAGTAGGATACCA
AAGAAGACCAATTATTGGATACAATGGTGCTTTGAACCTTGTCGATGGAATTACAAATACAATTCTCGATAAATACTACG
AAACTCAAGACTGGAAACTCCAACAGTAA

Upstream 100 bases:

>100_bases
TTTGAAGGATTCGTAAACATGGCAAGAGATATATACACAGCAATATACAACCCAGCCTGGAAATTAATGGAATTTGAAGA
AGAAGAGCCAGGTGATTCAA

Downstream 100 bases:

>100_bases
GCCCAAAATAAAGAGGAATACTATGGTTTTGAACCTTGATACAGAAAACCGGAAAATGCAAGATGGAAATAACGATGATG
ATTTTGACTTAGAAGTCAAA

Product: nitrogenase

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSELNVIKKERTAVINPIVTCQPLGAMYAVSGIERGMPLVHGSQGCSTFVRYGFARHFREPADIAVTSLHEDAAVFGGRK
NLISGLGNLAARFKPDVMGVVTTCSSEIIGDDVAGFIKTAKVEIAKKMGEEAANKIKIVQINTPSFVEHQFKGYDNAIKA
IVDTLAEPKDEENGKLNIIPGIVNPGDIREIKHMLSLMGVEGILLTDTSDPFDSPLRPSKADKNPYYQKGGTPLADLQDC
ANSLGTISLANYANSAPASLEKKYNMPSKVSEAPIGIQNTDSFIRTVKKFTGNDVTDEILDERGIVIDAMADVASRYLFG
RKVAIYGDPSITVGMARFVAELGMIPKVVCTGVKNEYFVNDLKKVAKESDEDIDALFGQDLRALDVYLKENPVDLMIGSS
DGRLMAKDLGIPLYRVGYPVYDRVGYQRRPIIGYNGALNLVDGITNTILDKYYETQDWKLQQ

Sequences:

>Translated_462_residues
MSELNVIKKERTAVINPIVTCQPLGAMYAVSGIERGMPLVHGSQGCSTFVRYGFARHFREPADIAVTSLHEDAAVFGGRK
NLISGLGNLAARFKPDVMGVVTTCSSEIIGDDVAGFIKTAKVEIAKKMGEEAANKIKIVQINTPSFVEHQFKGYDNAIKA
IVDTLAEPKDEENGKLNIIPGIVNPGDIREIKHMLSLMGVEGILLTDTSDPFDSPLRPSKADKNPYYQKGGTPLADLQDC
ANSLGTISLANYANSAPASLEKKYNMPSKVSEAPIGIQNTDSFIRTVKKFTGNDVTDEILDERGIVIDAMADVASRYLFG
RKVAIYGDPSITVGMARFVAELGMIPKVVCTGVKNEYFVNDLKKVAKESDEDIDALFGQDLRALDVYLKENPVDLMIGSS
DGRLMAKDLGIPLYRVGYPVYDRVGYQRRPIIGYNGALNLVDGITNTILDKYYETQDWKLQQ
>Mature_461_residues
SELNVIKKERTAVINPIVTCQPLGAMYAVSGIERGMPLVHGSQGCSTFVRYGFARHFREPADIAVTSLHEDAAVFGGRKN
LISGLGNLAARFKPDVMGVVTTCSSEIIGDDVAGFIKTAKVEIAKKMGEEAANKIKIVQINTPSFVEHQFKGYDNAIKAI
VDTLAEPKDEENGKLNIIPGIVNPGDIREIKHMLSLMGVEGILLTDTSDPFDSPLRPSKADKNPYYQKGGTPLADLQDCA
NSLGTISLANYANSAPASLEKKYNMPSKVSEAPIGIQNTDSFIRTVKKFTGNDVTDEILDERGIVIDAMADVASRYLFGR
KVAIYGDPSITVGMARFVAELGMIPKVVCTGVKNEYFVNDLKKVAKESDEDIDALFGQDLRALDVYLKENPVDLMIGSSD
GRLMAKDLGIPLYRVGYPVYDRVGYQRRPIIGYNGALNLVDGITNTILDKYYETQDWKLQQ

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFK_METMP (P71527)

Other databases:

- EMBL:   U75887
- EMBL:   BX950229
- PIR:   T10094
- RefSeq:   NP_987977.1
- ProteinModelPortal:   P71527
- SMR:   P71527
- GeneID:   2762384
- GenomeReviews:   BX950229_GR
- KEGG:   mmp:MMP0857
- NMPDR:   fig|267377.1.peg.857
- HOGENOM:   HBG336444
- OMA:   DGITNTI
- ProtClustDB:   CLSK878515
- BioCyc:   MMAR267377:MMP0857-MONOMER
- BRENDA:   1.18.6.1
- InterPro:   IPR000510
- InterPro:   IPR000318

Pfam domain/function: PF00148 Oxidored_nitro

EC number: =1.18.6.1

Molecular weight: Translated: 50503; Mature: 50372

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00699 NITROGENASE_1_1; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELNVIKKERTAVINPIVTCQPLGAMYAVSGIERGMPLVHGSQGCSTFVRYGFARHFRE
CCCCHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCC
PADIAVTSLHEDAAVFGGRKNLISGLGNLAARFKPDVMGVVTTCSSEIIGDDVAGFIKTA
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KVEIAKKMGEEAANKIKIVQINTPSFVEHQFKGYDNAIKAIVDTLAEPKDEENGKLNIIP
HHHHHHHHHHHHHCCEEEEEECCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEEEC
GIVNPGDIREIKHMLSLMGVEGILLTDTSDPFDSPLRPSKADKNPYYQKGGTPLADLQDC
CCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
ANSLGTISLANYANSAPASLEKKYNMPSKVSEAPIGIQNTDSFIRTVKKFTGNDVTDEIL
HHHHCCEEEHHCCCCCCHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHH
DERGIVIDAMADVASRYLFGRKVAIYGDPSITVGMARFVAELGMIPKVVCTGVKNEYFVN
HCCCCEEEHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHCCCCHHHHH
DLKKVAKESDEDIDALFGQDLRALDVYLKENPVDLMIGSSDGRLMAKDLGIPLYRVGYPV
HHHHHHHCCCCHHHHHHCCCHHHHHHEECCCCEEEEEECCCCCEEHHHCCCCHHHCCCCH
YDRVGYQRRPIIGYNGALNLVDGITNTILDKYYETQDWKLQQ
HHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCEECC
>Mature Secondary Structure 
SELNVIKKERTAVINPIVTCQPLGAMYAVSGIERGMPLVHGSQGCSTFVRYGFARHFRE
CCCHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHCCC
PADIAVTSLHEDAAVFGGRKNLISGLGNLAARFKPDVMGVVTTCSSEIIGDDVAGFIKTA
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KVEIAKKMGEEAANKIKIVQINTPSFVEHQFKGYDNAIKAIVDTLAEPKDEENGKLNIIP
HHHHHHHHHHHHHCCEEEEEECCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCEEEEEC
GIVNPGDIREIKHMLSLMGVEGILLTDTSDPFDSPLRPSKADKNPYYQKGGTPLADLQDC
CCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
ANSLGTISLANYANSAPASLEKKYNMPSKVSEAPIGIQNTDSFIRTVKKFTGNDVTDEIL
HHHHCCEEEHHCCCCCCHHHHHHCCCCCHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHH
DERGIVIDAMADVASRYLFGRKVAIYGDPSITVGMARFVAELGMIPKVVCTGVKNEYFVN
HCCCCEEEHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHCCCCHHHHH
DLKKVAKESDEDIDALFGQDLRALDVYLKENPVDLMIGSSDGRLMAKDLGIPLYRVGYPV
HHHHHHHCCCCHHHHHHCCCHHHHHHEECCCCEEEEEECCCCCEEHHHCCCCHHHCCCCH
YDRVGYQRRPIIGYNGALNLVDGITNTILDKYYETQDWKLQQ
HHHCCCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990309