Click here to switch to the map view.

The map label for this gene is nifD

Identifier: 45358419

GI number: 45358419

Start: 845702

End: 847135

Strand: Direct

Name: nifD

Synonym: MMP0856

Alternate gene names: 45358419

Gene position: 845702-847135 (Clockwise)

Preceding gene: 45358418

Following gene: 45358420

Centisome position: 50.91

GC content: 37.94

Gene sequence:

>1434_bases
ATGCCATTCTGTTTATTGGATGTAGATAAAGATATCCCTGAAAGAGAACAACACATTTACATCAAAGACTCAAAAGAACC
AAAAGGACACTGCAAACAAAGATGTAATACAAACACAATCCCTGGAAGTATGACTGAAAGAGGATGTGCTTTTGCAGGAG
TAAAAGGTGTGATTACAGGTGCAATAAAAGATGTATTGCACGTAGTACACTCGCCAGTAGGATGTACCGCTTATGGAAAC
GGTACAACAAAAAGATACCCAACAAGACCAGAAATGCCTGATGGAAGCGTATTTCCCGTTGAAAACTTCAACTTAAAACA
CATCGTTGGAACAGACTTAACTGAATCCGATGTTGTTTTTGGTGGAATGAACAAACTTAAAAAAGTAATTAGGGAAGCTT
CAAAAGAATTCCCATTCGTAAATGCAATCTATGTTTACGCAACATGTACAACAGGTCTTATTGGAGACGACTTAGATGCA
GTATGTAAAGAAATGCAAGCAGAACTTGGAAAAGATGTTGTAGCATTCAACGCTCCTGGATTTGCAGGACCAACACAATC
AAAAGGACACCACGTAGGAAACTACACGATATTTGAAAAATTGGTTGGAACAAAAGAACCTCCTAAAACAACCGATTACG
ACATCAACCTTATTGGAGAATATAACATCGATGGTGACTACTGGGTTCTCGAAAAATACTTCGAAGATATGGGCATTAAC
GTTCTGAGTAAATTCACTGGGGATGCAACCCACGGTGAACTCTGCTGGATGCACAAAGCAAAATTAAGCCTTGTAAGATG
CCAAAGATCTGCAACTTATGTAGCAAAATTAATCGAAGAAAAATACGGTGTTCCGTACCTGAAAGTAGATTTCTTCGGTC
CAGAATACTGTGCTGAAAACTTAAGAGCAGTAGGTAAATACTTCGGAAAAGAAATTGAAGCTGAAGCAGTTATCAAAAAA
GAAATGGAAAAAATTCAACCTGAACTTGATTTCTACAAATCAAAATTACAAGGTAAAAAAATCTGGATTTCAGCAGGAGG
TCCAAAAAGCTGGCACTTATCCAAACCAATAGAACAATACTTAGGAATGGATGTAGTAGCACTTTCAGGTCTCTTTGAGC
ACGAAGATGGATACGAAAAAATGCAAGAAAGGGCAAAAGACGGTACAATTATCATTGACGACCCGAACACCCTTGAAATG
GAAGAAGTTGTTGAAAAATACCAGCCAGAAATAGTTCTTGGTGGTATCAAAGAGAAATATTTCTTCCACAAATTAGGAGT
ACCTTCAGTAATGATACACTCTTACGAAAACGGCCCATACATTGGCTTTGAAGGATTCGTAAACATGGCAAGAGATATAT
ACACAGCAATATACAACCCAGCCTGGAAATTAATGGAATTTGAAGAAGAAGAGCCAGGTGATTCAAATGAGTGA

Upstream 100 bases:

>100_bases
GAAGATATAATCACAGTAAGAACCTCTGAAAGAGGAGAAGCTGCAATTTAAGCAAATAAACCCCAAAATTACCCTTTAAA
AATTTTTTAGGAGGGCAAAC

Downstream 100 bases:

>100_bases
GTTAAACGTAATCAAAAAAGAAAGAACCGCGGTAATTAACCCAATCGTAACATGCCAACCTTTAGGTGCAATGTACGCAG
TATCTGGAATTGAAAGAGGA

Product: nitrogenase subunit alpha

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MPFCLLDVDKDIPEREQHIYIKDSKEPKGHCKQRCNTNTIPGSMTERGCAFAGVKGVITGAIKDVLHVVHSPVGCTAYGN
GTTKRYPTRPEMPDGSVFPVENFNLKHIVGTDLTESDVVFGGMNKLKKVIREASKEFPFVNAIYVYATCTTGLIGDDLDA
VCKEMQAELGKDVVAFNAPGFAGPTQSKGHHVGNYTIFEKLVGTKEPPKTTDYDINLIGEYNIDGDYWVLEKYFEDMGIN
VLSKFTGDATHGELCWMHKAKLSLVRCQRSATYVAKLIEEKYGVPYLKVDFFGPEYCAENLRAVGKYFGKEIEAEAVIKK
EMEKIQPELDFYKSKLQGKKIWISAGGPKSWHLSKPIEQYLGMDVVALSGLFEHEDGYEKMQERAKDGTIIIDDPNTLEM
EEVVEKYQPEIVLGGIKEKYFFHKLGVPSVMIHSYENGPYIGFEGFVNMARDIYTAIYNPAWKLMEFEEEEPGDSNE

Sequences:

>Translated_477_residues
MPFCLLDVDKDIPEREQHIYIKDSKEPKGHCKQRCNTNTIPGSMTERGCAFAGVKGVITGAIKDVLHVVHSPVGCTAYGN
GTTKRYPTRPEMPDGSVFPVENFNLKHIVGTDLTESDVVFGGMNKLKKVIREASKEFPFVNAIYVYATCTTGLIGDDLDA
VCKEMQAELGKDVVAFNAPGFAGPTQSKGHHVGNYTIFEKLVGTKEPPKTTDYDINLIGEYNIDGDYWVLEKYFEDMGIN
VLSKFTGDATHGELCWMHKAKLSLVRCQRSATYVAKLIEEKYGVPYLKVDFFGPEYCAENLRAVGKYFGKEIEAEAVIKK
EMEKIQPELDFYKSKLQGKKIWISAGGPKSWHLSKPIEQYLGMDVVALSGLFEHEDGYEKMQERAKDGTIIIDDPNTLEM
EEVVEKYQPEIVLGGIKEKYFFHKLGVPSVMIHSYENGPYIGFEGFVNMARDIYTAIYNPAWKLMEFEEEEPGDSNE
>Mature_476_residues
PFCLLDVDKDIPEREQHIYIKDSKEPKGHCKQRCNTNTIPGSMTERGCAFAGVKGVITGAIKDVLHVVHSPVGCTAYGNG
TTKRYPTRPEMPDGSVFPVENFNLKHIVGTDLTESDVVFGGMNKLKKVIREASKEFPFVNAIYVYATCTTGLIGDDLDAV
CKEMQAELGKDVVAFNAPGFAGPTQSKGHHVGNYTIFEKLVGTKEPPKTTDYDINLIGEYNIDGDYWVLEKYFEDMGINV
LSKFTGDATHGELCWMHKAKLSLVRCQRSATYVAKLIEEKYGVPYLKVDFFGPEYCAENLRAVGKYFGKEIEAEAVIKKE
MEKIQPELDFYKSKLQGKKIWISAGGPKSWHLSKPIEQYLGMDVVALSGLFEHEDGYEKMQERAKDGTIIIDDPNTLEME
EVVEKYQPEIVLGGIKEKYFFHKLGVPSVMIHSYENGPYIGFEGFVNMARDIYTAIYNPAWKLMEFEEEEPGDSNE

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NIFD_METMP (P71526)

Other databases:

- EMBL:   U75887
- EMBL:   BX950229
- PIR:   T10093
- RefSeq:   NP_987976.1
- ProteinModelPortal:   P71526
- GeneID:   2761670
- GenomeReviews:   BX950229_GR
- KEGG:   mmp:MMP0856
- NMPDR:   fig|267377.1.peg.856
- HOGENOM:   HBG392742
- OMA:   FAGPSQS
- ProtClustDB:   CLSK878514
- BioCyc:   MMAR267377:MMP0856-MONOMER
- BRENDA:   1.18.6.1
- InterPro:   IPR000510
- InterPro:   IPR005974
- InterPro:   IPR010143
- InterPro:   IPR000318
- TIGRFAMs:   TIGR01284
- TIGRFAMs:   TIGR01862

Pfam domain/function: PF00148 Oxidored_nitro

EC number: =1.18.6.1

Molecular weight: Translated: 53633; Mature: 53502

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00699 NITROGENASE_1_1; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFCLLDVDKDIPEREQHIYIKDSKEPKGHCKQRCNTNTIPGSMTERGCAFAGVKGVITG
CCEEEEECCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHHH
AIKDVLHVVHSPVGCTAYGNGTTKRYPTRPEMPDGSVFPVENFNLKHIVGTDLTESDVVF
HHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEECCCCCCEEEEECCCCCCCCEEE
GGMNKLKKVIREASKEFPFVNAIYVYATCTTGLIGDDLDAVCKEMQAELGKDVVAFNAPG
CCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEECCCC
FAGPTQSKGHHVGNYTIFEKLVGTKEPPKTTDYDINLIGEYNIDGDYWVLEKYFEDMGIN
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCHH
VLSKFTGDATHGELCWMHKAKLSLVRCQRSATYVAKLIEEKYGVPYLKVDFFGPEYCAEN
HHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHH
LRAVGKYFGKEIEAEAVIKKEMEKIQPELDFYKSKLQGKKIWISAGGPKSWHLSKPIEQY
HHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHH
LGMDVVALSGLFEHEDGYEKMQERAKDGTIIIDDPNTLEMEEVVEKYQPEIVLGGIKEKY
HCCHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCEEECCCHHHH
FFHKLGVPSVMIHSYENGPYIGFEGFVNMARDIYTAIYNPAWKLMEFEEEEPGDSNE
HHHHCCCCEEEEEECCCCCEECHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
PFCLLDVDKDIPEREQHIYIKDSKEPKGHCKQRCNTNTIPGSMTERGCAFAGVKGVITG
CEEEEECCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCCCCCCHHCCCHHHHHHHHHHH
AIKDVLHVVHSPVGCTAYGNGTTKRYPTRPEMPDGSVFPVENFNLKHIVGTDLTESDVVF
HHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCEECCCCCCEEEEECCCCCCCCEEE
GGMNKLKKVIREASKEFPFVNAIYVYATCTTGLIGDDLDAVCKEMQAELGKDVVAFNAPG
CCHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEECCCC
FAGPTQSKGHHVGNYTIFEKLVGTKEPPKTTDYDINLIGEYNIDGDYWVLEKYFEDMGIN
CCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHCHH
VLSKFTGDATHGELCWMHKAKLSLVRCQRSATYVAKLIEEKYGVPYLKVDFFGPEYCAEN
HHHHHCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHH
LRAVGKYFGKEIEAEAVIKKEMEKIQPELDFYKSKLQGKKIWISAGGPKSWHLSKPIEQY
HHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHH
LGMDVVALSGLFEHEDGYEKMQERAKDGTIIIDDPNTLEMEEVVEKYQPEIVLGGIKEKY
HCCHHHHHHHHHHCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHCCCEEECCCHHHH
FFHKLGVPSVMIHSYENGPYIGFEGFVNMARDIYTAIYNPAWKLMEFEEEEPGDSNE
HHHHCCCCEEEEEECCCCCEECHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990309