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The map label for this gene is miaA

Identifier: 42523071

GI number: 42523071

Start: 1491638

End: 1492564

Strand: Direct

Name: miaA

Synonym: Bd1565

Alternate gene names: 42523071

Gene position: 1491638-1492564 (Clockwise)

Preceding gene: 42523070

Following gene: 42523072

Centisome position: 39.43

GC content: 53.18

Gene sequence:

>927_bases
ATGGCGAAAAGGCATGTGATTTTTGTTGTCGGCTCCACGGCCACCGGGAAATCCGAGTGGGCGCTGAAGCTTGCGCAAGA
ATTCAACGGCGTCATCGTCAATTGTGATTCGGTTCAGCTGTATAAGAAACTGGATATCGGTTCGGCCAAACCCAGCAAGG
CAGAGCAGGCCTTGGTGCCGCACTATCTGCTGGATTATGTAAATCCGCCGGAAGAAATGACGGCCGGGAATTACTGCCGT
GATTTTTACGCCATTCTAGAAGAGATCCCCGCCGACAAGCCGGTGTTTGTGGTGGGCGGTACTGGTTTTTATTTTATGGC
CATTGAAAAGGGCATGTATCCGGTGATCCCGGTCCCGGTAGAAATTCAGGCCCAGGTGGCCCTTGAGCTTGAAACTGAAG
AAGGCGCCATCCGCCTGCACGCCGAAATGATGAAGGCCGATCCTGAATACGGCGCCAAGATTCATCTGGCAGATCGCTAT
CGTATCGGGCGGGCGATTGAACTGATTCGCAGCCAGGGAAAAAGTGTCACGCAGATTCAAGCCGAATTTGAGTCCCAGCG
AAAGCCGTTCCCGTTTCCGCTGTTGAAGATCGGTCCGAGTTGGGATCGCGAAGTGCTGCGCGAACGCATCGGGCAGCGCG
TGGAAAAGATGCTCGCTGCCGGATTGATTGAAGAGGTTCAGGGGCTTCTCGATGAAGGTCTGGCTTCATGGGCACCGATC
AGCAGTGTGGGCTATAAAGAGACACTCGAATATCTGCGTGGGGGAATAAGTCTTTCGCAGCTGCAGGAAGAGATCACGAC
AAACACTCACCAGCTCGCCAAAAGACAGAGAACTTGGTTTCAGCGAGACAAAGACATTCAGTGGTTCGATGGGGCCTCGG
GTTTTGCTGAGGTTCGAACCGTGGTCGAGAAATTTCTCAAACCTTGA

Upstream 100 bases:

>100_bases
TGGACATGTTCCCGCTTTCAAGCTTCTGTCCTCACGGTCGCCCGGTCAGTGTGGAGTACCCGTACTACAAGCTGGAAAAA
GATTTCGGACGAATAGTTTA

Downstream 100 bases:

>100_bases
CCCTGATTCGGTGGAAAGCCGAGAATAGAGGTAATTATGAAAAGCATGACTGGGTACGGCACCGCAAGAGTTCAGACTAA
AGACGTCACTGTGGAAGTGA

Product: hypothetical protein

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MAKRHVIFVVGSTATGKSEWALKLAQEFNGVIVNCDSVQLYKKLDIGSAKPSKAEQALVPHYLLDYVNPPEEMTAGNYCR
DFYAILEEIPADKPVFVVGGTGFYFMAIEKGMYPVIPVPVEIQAQVALELETEEGAIRLHAEMMKADPEYGAKIHLADRY
RIGRAIELIRSQGKSVTQIQAEFESQRKPFPFPLLKIGPSWDREVLRERIGQRVEKMLAAGLIEEVQGLLDEGLASWAPI
SSVGYKETLEYLRGGISLSQLQEEITTNTHQLAKRQRTWFQRDKDIQWFDGASGFAEVRTVVEKFLKP

Sequences:

>Translated_308_residues
MAKRHVIFVVGSTATGKSEWALKLAQEFNGVIVNCDSVQLYKKLDIGSAKPSKAEQALVPHYLLDYVNPPEEMTAGNYCR
DFYAILEEIPADKPVFVVGGTGFYFMAIEKGMYPVIPVPVEIQAQVALELETEEGAIRLHAEMMKADPEYGAKIHLADRY
RIGRAIELIRSQGKSVTQIQAEFESQRKPFPFPLLKIGPSWDREVLRERIGQRVEKMLAAGLIEEVQGLLDEGLASWAPI
SSVGYKETLEYLRGGISLSQLQEEITTNTHQLAKRQRTWFQRDKDIQWFDGASGFAEVRTVVEKFLKP
>Mature_307_residues
AKRHVIFVVGSTATGKSEWALKLAQEFNGVIVNCDSVQLYKKLDIGSAKPSKAEQALVPHYLLDYVNPPEEMTAGNYCRD
FYAILEEIPADKPVFVVGGTGFYFMAIEKGMYPVIPVPVEIQAQVALELETEEGAIRLHAEMMKADPEYGAKIHLADRYR
IGRAIELIRSQGKSVTQIQAEFESQRKPFPFPLLKIGPSWDREVLRERIGQRVEKMLAAGLIEEVQGLLDEGLASWAPIS
SVGYKETLEYLRGGISLSQLQEEITTNTHQLAKRQRTWFQRDKDIQWFDGASGFAEVRTVVEKFLKP

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=311, Percent_Identity=31.1897106109325, Blast_Score=119, Evalue=4e-27,
Organism=Escherichia coli, GI1790613, Length=287, Percent_Identity=34.8432055749129, Blast_Score=161, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI25144712, Length=274, Percent_Identity=25.1824817518248, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324848, Length=296, Percent_Identity=27.3648648648649, Blast_Score=112, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24649830, Length=277, Percent_Identity=33.2129963898917, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_BDEBA (Q6MMQ9)

Other databases:

- EMBL:   BX842650
- RefSeq:   NP_968451.1
- ProteinModelPortal:   Q6MMQ9
- SMR:   Q6MMQ9
- GeneID:   2735451
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd1565
- NMPDR:   fig|264462.1.peg.1421
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   Q6MMQ9
- BioCyc:   BBAC264462:BD1565-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34706; Mature: 34575

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRHVIFVVGSTATGKSEWALKLAQEFNGVIVNCDSVQLYKKLDIGSAKPSKAEQALVP
CCCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCEEHHHHCCCCCCCCCHHHHHHHH
HYLLDYVNPPEEMTAGNYCRDFYAILEEIPADKPVFVVGGTGFYFMAIEKGMYPVIPVPV
HHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEECCCCCEECCCE
EIQAQVALELETEEGAIRLHAEMMKADPEYGAKIHLADRYRIGRAIELIRSQGKSVTQIQ
EEEEEEEEEEECCCCCEEEHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHH
AEFESQRKPFPFPLLKIGPSWDREVLRERIGQRVEKMLAAGLIEEVQGLLDEGLASWAPI
HHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SSVGYKETLEYLRGGISLSQLQEEITTNTHQLAKRQRTWFQRDKDIQWFDGASGFAEVRT
CCCCHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHH
VVEKFLKP
HHHHHCCC
>Mature Secondary Structure 
AKRHVIFVVGSTATGKSEWALKLAQEFNGVIVNCDSVQLYKKLDIGSAKPSKAEQALVP
CCCEEEEEEECCCCCCHHHHHHHHHHCCCEEEECCCEEHHHHCCCCCCCCCHHHHHHHH
HYLLDYVNPPEEMTAGNYCRDFYAILEEIPADKPVFVVGGTGFYFMAIEKGMYPVIPVPV
HHHHHCCCCHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEECCCCCEECCCE
EIQAQVALELETEEGAIRLHAEMMKADPEYGAKIHLADRYRIGRAIELIRSQGKSVTQIQ
EEEEEEEEEEECCCCCEEEHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHCCCHHHHHH
AEFESQRKPFPFPLLKIGPSWDREVLRERIGQRVEKMLAAGLIEEVQGLLDEGLASWAPI
HHHHHCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SSVGYKETLEYLRGGISLSQLQEEITTNTHQLAKRQRTWFQRDKDIQWFDGASGFAEVRT
CCCCHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHH
VVEKFLKP
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA