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The map label for this gene is carA [H]

Identifier: 42522428

GI number: 42522428

Start: 802162

End: 803226

Strand: Direct

Name: carA [H]

Synonym: Bd0856

Alternate gene names: 42522428

Gene position: 802162-803226 (Clockwise)

Preceding gene: 42522427

Following gene: 42522429

Centisome position: 21.2

GC content: 52.86

Gene sequence:

>1065_bases
ATGCGCGGCTATCTGGTGCTTGAAGGTGGGGAAGTCTACACCGGTCAATGGCAGGGTGGTGAAGATCGCGCCGGCGAGGT
TGTGTTCAACACCTCCCACTCCGGTTACGAAGAAATTGCCACCGATCCGTCTTATTTTTCTCAAATTGTGGTGATGACCG
CCCCCATGCAGGGGAATTATGGGGTTGAAGACGCTGTCTGGGAATCGCGGAAGCTCTGGATCGAGGGCTTCATCTGTCTG
GAAGTTCAGGACAGCGAACGCGACCATGCCTGGAAAAAACGACTGACTGACAATAAAATTCCGATGCTCACCGAACTGGA
CACGCGGGCTTTGGCTTTGCGATTGCGTCAGGGCGGAACTCCCTGGGGGGCACTGGTTCAGGCCGGCTCCGAGGCTGAAG
CTTTGAAAAAGGCAGACAGCCTGATAGCCGCAAAAAAGACTGTTGATAAAGACTGGGTGTACATCGTTTCCCGCAAAGAA
GCAGAAACCCGCCGCGGTGAAAACATGGTGGGCCCGAAGGTGGCGGTTCTGGATTTTGGCAGCAAAGAAAACATCCTGCG
TGAACTGCAAAACCGCTGTTCTGAAATCAAGATTTTCAACAGCCGCTCCTCGGTTCAGGAGATTCTGGCTTACAATCCGG
ACGGGATCATGCTGACCAACGGCCCGGGCGATCCGGCGGACGTAAAAGTGGCCATCGGCACAGTTCGCGAACTTTTGGGT
GTAAAACCGATCTTTGGTATCTGCATGGGACACCAGATTCTGGGGCTGGCCCTGGGGGCAAAGACCTACAAGTTAAAATT
CGGCCATCGTGGGTCCAATCATCCGATTCGCGACACCCTGCTTAATCAGATTTATATGACCAGCCAGAACCACGGATACG
CCGTGGAAGAGGCTTCTTTGCCTTCTGACGTGAAGGTCACCCATGTGAACCTCAATGACGGCACTGTGGCAGGTTTTTAT
AGTGAAAAACGCAAGTGTTTAGGAATACAATATCACCCGGAAAGTTGTCCTGGGCCGCATGAGGCTTCAGGGCTGTTTAG
CTACTTTGTAGAGCGGATGATATGA

Upstream 100 bases:

>100_bases
ACGTTCTGAAGGATCCGCGCTGCAAGGTGCTGGATCAGGTTTCCAATGGTGTTTTGATTCGTCAAAGTCTGATTCAAATG
ACGTTGAAAGAGGGGGAGTG

Downstream 100 bases:

>100_bases
ACGAATACGAAAAACTGATCGAAAAAATTCTTAACCACTTTGTCAGCGACGCATTCAAGGACGAATTGGCCATGGCCAAA
AAAGAGTTCTTTGAAAACGC

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MRGYLVLEGGEVYTGQWQGGEDRAGEVVFNTSHSGYEEIATDPSYFSQIVVMTAPMQGNYGVEDAVWESRKLWIEGFICL
EVQDSERDHAWKKRLTDNKIPMLTELDTRALALRLRQGGTPWGALVQAGSEAEALKKADSLIAAKKTVDKDWVYIVSRKE
AETRRGENMVGPKVAVLDFGSKENILRELQNRCSEIKIFNSRSSVQEILAYNPDGIMLTNGPGDPADVKVAIGTVRELLG
VKPIFGICMGHQILGLALGAKTYKLKFGHRGSNHPIRDTLLNQIYMTSQNHGYAVEEASLPSDVKVTHVNLNDGTVAGFY
SEKRKCLGIQYHPESCPGPHEASGLFSYFVERMI

Sequences:

>Translated_354_residues
MRGYLVLEGGEVYTGQWQGGEDRAGEVVFNTSHSGYEEIATDPSYFSQIVVMTAPMQGNYGVEDAVWESRKLWIEGFICL
EVQDSERDHAWKKRLTDNKIPMLTELDTRALALRLRQGGTPWGALVQAGSEAEALKKADSLIAAKKTVDKDWVYIVSRKE
AETRRGENMVGPKVAVLDFGSKENILRELQNRCSEIKIFNSRSSVQEILAYNPDGIMLTNGPGDPADVKVAIGTVRELLG
VKPIFGICMGHQILGLALGAKTYKLKFGHRGSNHPIRDTLLNQIYMTSQNHGYAVEEASLPSDVKVTHVNLNDGTVAGFY
SEKRKCLGIQYHPESCPGPHEASGLFSYFVERMI
>Mature_354_residues
MRGYLVLEGGEVYTGQWQGGEDRAGEVVFNTSHSGYEEIATDPSYFSQIVVMTAPMQGNYGVEDAVWESRKLWIEGFICL
EVQDSERDHAWKKRLTDNKIPMLTELDTRALALRLRQGGTPWGALVQAGSEAEALKKADSLIAAKKTVDKDWVYIVSRKE
AETRRGENMVGPKVAVLDFGSKENILRELQNRCSEIKIFNSRSSVQEILAYNPDGIMLTNGPGDPADVKVAIGTVRELLG
VKPIFGICMGHQILGLALGAKTYKLKFGHRGSNHPIRDTLLNQIYMTSQNHGYAVEEASLPSDVKVTHVNLNDGTVAGFY
SEKRKCLGIQYHPESCPGPHEASGLFSYFVERMI

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=370, Percent_Identity=36.7567567567568, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI169790915, Length=368, Percent_Identity=33.1521739130435, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI21361331, Length=368, Percent_Identity=33.1521739130435, Blast_Score=172, Evalue=6e-43,
Organism=Escherichia coli, GI1786215, Length=372, Percent_Identity=40.0537634408602, Blast_Score=265, Evalue=4e-72,
Organism=Escherichia coli, GI1789760, Length=143, Percent_Identity=28.6713286713287, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=372, Percent_Identity=35.4838709677419, Blast_Score=205, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6324878, Length=377, Percent_Identity=34.7480106100796, Blast_Score=194, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6322331, Length=386, Percent_Identity=34.1968911917098, Blast_Score=191, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6322638, Length=161, Percent_Identity=32.2981366459627, Blast_Score=73, Evalue=6e-14,
Organism=Drosophila melanogaster, GI45555749, Length=375, Percent_Identity=35.4666666666667, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24642586, Length=375, Percent_Identity=35.4666666666667, Blast_Score=202, Evalue=2e-52,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 39256; Mature: 39256

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGYLVLEGGEVYTGQWQGGEDRAGEVVFNTSHSGYEEIATDPSYFSQIVVMTAPMQGNY
CCCEEEEECCEEEEECCCCCCCCCCEEEEECCCCCHHHHCCCHHHHCEEEEEEECCCCCC
GVEDAVWESRKLWIEGFICLEVQDSERDHAWKKRLTDNKIPMLTELDTRALALRLRQGGT
CCHHHHHCCCEEEEEEEEEEEEECCCHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCC
PWGALVQAGSEAEALKKADSLIAAKKTVDKDWVYIVSRKEAETRRGENMVGPKVAVLDFG
CHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHCCCCCCCCEEEEEECC
SKENILRELQNRCSEIKIFNSRSSVQEILAYNPDGIMLTNGPGDPADVKVAIGTVRELLG
CHHHHHHHHHHHHHHEEEECCHHHHHHHHHCCCCCEEEECCCCCCCEEEEHHHHHHHHHC
VKPIFGICMGHQILGLALGAKTYKLKFGHRGSNHPIRDTLLNQIYMTSQNHGYAVEEASL
CCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHEEECCCCCEEEECCCC
PSDVKVTHVNLNDGTVAGFYSEKRKCLGIQYHPESCPGPHEASGLFSYFVERMI
CCCCEEEEEECCCCEEEEEHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MRGYLVLEGGEVYTGQWQGGEDRAGEVVFNTSHSGYEEIATDPSYFSQIVVMTAPMQGNY
CCCEEEEECCEEEEECCCCCCCCCCEEEEECCCCCHHHHCCCHHHHCEEEEEEECCCCCC
GVEDAVWESRKLWIEGFICLEVQDSERDHAWKKRLTDNKIPMLTELDTRALALRLRQGGT
CCHHHHHCCCEEEEEEEEEEEEECCCHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCC
PWGALVQAGSEAEALKKADSLIAAKKTVDKDWVYIVSRKEAETRRGENMVGPKVAVLDFG
CHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHCCCCCCCCEEEEEECC
SKENILRELQNRCSEIKIFNSRSSVQEILAYNPDGIMLTNGPGDPADVKVAIGTVRELLG
CHHHHHHHHHHHHHHEEEECCHHHHHHHHHCCCCCEEEECCCCCCCEEEEHHHHHHHHHC
VKPIFGICMGHQILGLALGAKTYKLKFGHRGSNHPIRDTLLNQIYMTSQNHGYAVEEASL
CCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHEEECCCCCEEEECCCC
PSDVKVTHVNLNDGTVAGFYSEKRKCLGIQYHPESCPGPHEASGLFSYFVERMI
CCCCEEEEEECCCCEEEEEHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA