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The map label for this gene is pyrB [H]

Identifier: 42522427

GI number: 42522427

Start: 801260

End: 802162

Strand: Direct

Name: pyrB [H]

Synonym: Bd0855

Alternate gene names: 42522427

Gene position: 801260-802162 (Clockwise)

Preceding gene: 42522426

Following gene: 42522428

Centisome position: 21.18

GC content: 51.05

Gene sequence:

>903_bases
ATGTCGTCTAGGAAAAATTTCTCCATTCTTGATCTTCGTTCGCTTGAAAAAACAAAAATCGATTTCCTATTCTCTATTGC
CGACTCTGTTGCGGCATCAAAATCGGCTCCTATTCAGGGCTTCGGAAAAACCGGAGCCCTTTTGTTTTTTGAAGCCAGCA
CTCGCACCCGCATGAGCTTTGAAACGGCCTGTGCTCGTTTGGGAATCCATCCGTTGCGCCTGGATGGCAGCGCGGGCAGC
AGTCTTGAAAAAGGCGAAACCCTGGAAGACACCGTTTTGAATGTCGAGGCCATGAAGCCAGCATTTTTGGTGATCCGCTG
TGGGGATGATCTGGATCTGAAAGATCTTTCCGAAAAAATCAGCACCCCGATTCTGAATGCCGGCTGGGGAAAGAAGGGGC
ATCCGACGCAGGCGTTGCTGGATGCTTACACACTTCGCAAGCATCTGGGCACGTGTTCAGGCCAAAAGCTTTTGATCGTG
GGCGATGTTCGCCACAGCCGGGTTGCGGCCTCTCACTTTGAGCTGGCAGAAAAGCTGGGTTACGAAGTGGCTCTGTGTGG
TCCAAAAGAATTTCTTCCGGCCCAGCCACCATGTCGTGTGTTTGCCACTTTGAAGGAAGGCCTTCAGTGGTCCACAGCGA
CGATGGCGTTGCGAGTGCAGTTGGAGCGTCACGAGAATAAATATTCTTTGGCGGACTATCGTGACCAGTATGGTTTCACT
GCCAAAAATCTGCAGTCCCTTTCGGACCAGGCCTTGATCATGCATCCGGGTCCGATCAATCAAGGGATTGAGCTTGATTC
CGACGTTCTGAAGGATCCGCGCTGCAAGGTGCTGGATCAGGTTTCCAATGGTGTTTTGATTCGTCAAAGTCTGATTCAAA
TGACGTTGAAAGAGGGGGAGTGA

Upstream 100 bases:

>100_bases
AGAAATGGGGTGGCGATCGCTATCCTTCAGTGCGTTGGGACCGAGTTTTTTCTACGGTTCATTGACAAGGTCTGCCCAAG
TACCTAGTCTTGGGTTCTAG

Downstream 100 bases:

>100_bases
TGCGCGGCTATCTGGTGCTTGAAGGTGGGGAAGTCTACACCGGTCAATGGCAGGGTGGTGAAGATCGCGCCGGCGAGGTT
GTGTTCAACACCTCCCACTC

Product: aspartate carbamoyltransferase

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 300; Mature: 299

Protein sequence:

>300_residues
MSSRKNFSILDLRSLEKTKIDFLFSIADSVAASKSAPIQGFGKTGALLFFEASTRTRMSFETACARLGIHPLRLDGSAGS
SLEKGETLEDTVLNVEAMKPAFLVIRCGDDLDLKDLSEKISTPILNAGWGKKGHPTQALLDAYTLRKHLGTCSGQKLLIV
GDVRHSRVAASHFELAEKLGYEVALCGPKEFLPAQPPCRVFATLKEGLQWSTATMALRVQLERHENKYSLADYRDQYGFT
AKNLQSLSDQALIMHPGPINQGIELDSDVLKDPRCKVLDQVSNGVLIRQSLIQMTLKEGE

Sequences:

>Translated_300_residues
MSSRKNFSILDLRSLEKTKIDFLFSIADSVAASKSAPIQGFGKTGALLFFEASTRTRMSFETACARLGIHPLRLDGSAGS
SLEKGETLEDTVLNVEAMKPAFLVIRCGDDLDLKDLSEKISTPILNAGWGKKGHPTQALLDAYTLRKHLGTCSGQKLLIV
GDVRHSRVAASHFELAEKLGYEVALCGPKEFLPAQPPCRVFATLKEGLQWSTATMALRVQLERHENKYSLADYRDQYGFT
AKNLQSLSDQALIMHPGPINQGIELDSDVLKDPRCKVLDQVSNGVLIRQSLIQMTLKEGE
>Mature_299_residues
SSRKNFSILDLRSLEKTKIDFLFSIADSVAASKSAPIQGFGKTGALLFFEASTRTRMSFETACARLGIHPLRLDGSAGSS
LEKGETLEDTVLNVEAMKPAFLVIRCGDDLDLKDLSEKISTPILNAGWGKKGHPTQALLDAYTLRKHLGTCSGQKLLIVG
DVRHSRVAASHFELAEKLGYEVALCGPKEFLPAQPPCRVFATLKEGLQWSTATMALRVQLERHENKYSLADYRDQYGFTA
KNLQSLSDQALIMHPGPINQGIELDSDVLKDPRCKVLDQVSNGVLIRQSLIQMTLKEGE

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=305, Percent_Identity=30.8196721311475, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI38788445, Length=155, Percent_Identity=34.8387096774194, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI2367364, Length=304, Percent_Identity=30.2631578947368, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1790703, Length=166, Percent_Identity=31.9277108433735, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=321, Percent_Identity=29.595015576324, Blast_Score=108, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6322331, Length=317, Percent_Identity=28.391167192429, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24642586, Length=306, Percent_Identity=28.7581699346405, Blast_Score=108, Evalue=4e-24,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 32993; Mature: 32862

Theoretical pI: Translated: 7.85; Mature: 7.85

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRKNFSILDLRSLEKTKIDFLFSIADSVAASKSAPIQGFGKTGALLFFEASTRTRMSF
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHH
ETACARLGIHPLRLDGSAGSSLEKGETLEDTVLNVEAMKPAFLVIRCGDDLDLKDLSEKI
HHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHEEHEECCCEEEEEECCCCCCHHHHHHHH
STPILNAGWGKKGHPTQALLDAYTLRKHLGTCSGQKLLIVGDVRHSRVAASHFELAEKLG
CCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCC
YEVALCGPKEFLPAQPPCRVFATLKEGLQWSTATMALRVQLERHENKYSLADYRDQYGFT
CEEEEECCHHCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCHHHHHHHCCCC
AKNLQSLSDQALIMHPGPINQGIELDSDVLKDPRCKVLDQVSNGVLIRQSLIQMTLKEGE
HHHHHHCCCCEEEECCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSRKNFSILDLRSLEKTKIDFLFSIADSVAASKSAPIQGFGKTGALLFFEASTRTRMSF
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHHH
ETACARLGIHPLRLDGSAGSSLEKGETLEDTVLNVEAMKPAFLVIRCGDDLDLKDLSEKI
HHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHEEHEECCCEEEEEECCCCCCHHHHHHHH
STPILNAGWGKKGHPTQALLDAYTLRKHLGTCSGQKLLIVGDVRHSRVAASHFELAEKLG
CCCEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCC
YEVALCGPKEFLPAQPPCRVFATLKEGLQWSTATMALRVQLERHENKYSLADYRDQYGFT
CEEEEECCHHCCCCCCCHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCHHHHHHHCCCC
AKNLQSLSDQALIMHPGPINQGIELDSDVLKDPRCKVLDQVSNGVLIRQSLIQMTLKEGE
HHHHHHCCCCEEEECCCCCCCCCCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA