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The map label for this gene is lepB [H]

Identifier: 42522426

GI number: 42522426

Start: 800550

End: 801224

Strand: Direct

Name: lepB [H]

Synonym: Bd0854

Alternate gene names: 42522426

Gene position: 800550-801224 (Clockwise)

Preceding gene: 42522425

Following gene: 42522427

Centisome position: 21.16

GC content: 51.7

Gene sequence:

>675_bases
ATGAATCGTTGGCGGGAATATCTAACAACTTTGGTTTTGGCAGTGGTGTGTGCACTTTTTGTGCGTCACTTTCTGGTCAC
CGCTTATAAAGTCCCGACAGGTTCCATGCAACCGGCTCTAAAGCCCGGTGATTTCATCTTTGCCTCACGTGTGTCTTACG
GTGTGCAGATTCCGTTTGCTTCCAAGCGCTGGAATCTGACTTTGCCAGCTCGCGGGGATATCGTGGTGTTCAGTTATCCC
AATCAGCCGTCAGTCACTTACGTCAAACGCGTGGTGGGTTTACCTGGGGATCGGGTGCAGATCGTAAAAGGCCGTCTCGT
TCTGAATGACGAACCGGTGTCTTATGAAAAATTGGATGCGGCTTCCGATAATCCCAATGCGGAACTCTTTGATATCTTCA
AAGAGACTTCCGGTGCCGACTCCTGGCGTGTGATCTTCCAGAAAACTCCAGACGACAAAGATTTCGGCCCGCTGGTTGTG
CCTCCAGGAGAGGTTTTCCTGTTGGGGGATAATCGTGATGCCAGTGACGACTCGCGCTATTGGGGCACGGTTCCTATGAC
CCAGGTGGTGGGCCGCGTGGCTTTAATTTGGCTTTCGCTGGATTGGCAGCAGAAATGGGGTGGCGATCGCTATCCTTCAG
TGCGTTGGGACCGAGTTTTTTCTACGGTTCATTGA

Upstream 100 bases:

>100_bases
CGTTTATTTAAGACCTTGGATTAATAGGTCGTCTAAGAAATAATTGAAAGCCCTCAGAATTGCTTTGAAGGTCTCGCCCA
AGTCCGTTTAGGATTTAGGG

Downstream 100 bases:

>100_bases
CAAGGTCTGCCCAAGTACCTAGTCTTGGGTTCTAGATGTCGTCTAGGAAAAATTTCTCCATTCTTGATCTTCGTTCGCTT
GAAAAAACAAAAATCGATTT

Product: hypothetical protein

Products: NA

Alternate protein names: SPase I; Leader peptidase I [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MNRWREYLTTLVLAVVCALFVRHFLVTAYKVPTGSMQPALKPGDFIFASRVSYGVQIPFASKRWNLTLPARGDIVVFSYP
NQPSVTYVKRVVGLPGDRVQIVKGRLVLNDEPVSYEKLDAASDNPNAELFDIFKETSGADSWRVIFQKTPDDKDFGPLVV
PPGEVFLLGDNRDASDDSRYWGTVPMTQVVGRVALIWLSLDWQQKWGGDRYPSVRWDRVFSTVH

Sequences:

>Translated_224_residues
MNRWREYLTTLVLAVVCALFVRHFLVTAYKVPTGSMQPALKPGDFIFASRVSYGVQIPFASKRWNLTLPARGDIVVFSYP
NQPSVTYVKRVVGLPGDRVQIVKGRLVLNDEPVSYEKLDAASDNPNAELFDIFKETSGADSWRVIFQKTPDDKDFGPLVV
PPGEVFLLGDNRDASDDSRYWGTVPMTQVVGRVALIWLSLDWQQKWGGDRYPSVRWDRVFSTVH
>Mature_224_residues
MNRWREYLTTLVLAVVCALFVRHFLVTAYKVPTGSMQPALKPGDFIFASRVSYGVQIPFASKRWNLTLPARGDIVVFSYP
NQPSVTYVKRVVGLPGDRVQIVKGRLVLNDEPVSYEKLDAASDNPNAELFDIFKETSGADSWRVIFQKTPDDKDFGPLVV
PPGEVFLLGDNRDASDDSRYWGTVPMTQVVGRVALIWLSLDWQQKWGGDRYPSVRWDRVFSTVH

Specific function: Unknown

COG id: COG0681

COG function: function code U; Signal peptidase I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S26 family [H]

Homologues:

Organism=Escherichia coli, GI1788921, Length=249, Percent_Identity=32.5301204819277, Blast_Score=130, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000223
- InterPro:   IPR019758
- InterPro:   IPR019757
- InterPro:   IPR019756
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056 [H]

Pfam domain/function: PF00717 Peptidase_S24 [H]

EC number: =3.4.21.89 [H]

Molecular weight: Translated: 25370; Mature: 25370

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS00760 SPASE_I_2 ; PS00761 SPASE_I_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRWREYLTTLVLAVVCALFVRHFLVTAYKVPTGSMQPALKPGDFIFASRVSYGVQIPFA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEECCCCCEECCCC
SKRWNLTLPARGDIVVFSYPNQPSVTYVKRVVGLPGDRVQIVKGRLVLNDEPVSYEKLDA
CCCEEEECCCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEEEEEEECCCCCCHHHCCC
ASDNPNAELFDIFKETSGADSWRVIFQKTPDDKDFGPLVVPPGEVFLLGDNRDASDDSRY
CCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEECCCCEEEEECCCCCCCCCCE
WGTVPMTQVVGRVALIWLSLDWQQKWGGDRYPSVRWDRVFSTVH
ECCCCHHHHHHHHEEEEEEECHHHHCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MNRWREYLTTLVLAVVCALFVRHFLVTAYKVPTGSMQPALKPGDFIFASRVSYGVQIPFA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEECCCCCEECCCC
SKRWNLTLPARGDIVVFSYPNQPSVTYVKRVVGLPGDRVQIVKGRLVLNDEPVSYEKLDA
CCCEEEECCCCCCEEEEECCCCCCHHHHHHHHCCCCCEEEEEEEEEEECCCCCCHHHCCC
ASDNPNAELFDIFKETSGADSWRVIFQKTPDDKDFGPLVVPPGEVFLLGDNRDASDDSRY
CCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCEEECCCCEEEEECCCCCCCCCCE
WGTVPMTQVVGRVALIWLSLDWQQKWGGDRYPSVRWDRVFSTVH
ECCCCHHHHHHHHEEEEEEECHHHHCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1546969 [H]