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The map label for this gene is metK

Identifier: 42522422

GI number: 42522422

Start: 795930

End: 797093

Strand: Direct

Name: metK

Synonym: Bd0850

Alternate gene names: 42522422

Gene position: 795930-797093 (Clockwise)

Preceding gene: 42522421

Following gene: 42522423

Centisome position: 21.04

GC content: 52.23

Gene sequence:

>1164_bases
GTGAAAAACTATCTCTTTACGAGTGAATCTGTATCCGAAGGTCACCCCGACAAAATGGCCGATCAAATCTCTGACGGTAT
TCTGGATGCCATCCTGGCTCAGGATCCGAAGGGCCGTGTTGCCTGTGAAACCCTGCTGACAACCGGTTTGGTGGTTGTTG
CCGGTGAGATCACAACTTCGGCAAAAGTGAACTTCTCTGAAGTGGCTCGTGATGTTGTAAAACGCATTGGCTACGACCAC
TCTGACAAGGGCTTTGACTACAAAACATGCGGCGTGATGATTGCCGTGGGTCAGCAGTCCCCGGATATCGCGGTGGGCGT
TAAAGAAACTCTTTCTGACAATCAAGGCGCTGGTGATCAGGGTCTGATGTTCGGTTACGCTGTGAACGAAACTCCAGAGC
TGATGCCTTTGAGCATCGCGATGTCTCACAAACTGGTGAAAGACCTGGCGGCTCTTCGCAAAGCCAACAAAGTGGACTGG
TTGCGCCCGGATGCAAAATCCCAAGTGACTGTTCAGTACGAAAACGGCATTGCAAAACGCATCGATGCAGTGGTTATCTC
CACTCAGCACGCCGACAGTGTTTCCAACTCCACAATCCAGGAGTTCATCACTGAAGAGCTGATCAAAAAATCCATCCCAG
GCAACTGGATCGACTCCAAAACCAAATTCTTCATCAACCCAACAGGCCGTTTCGTAACGGGTGGTCCAATGGGTGATGCG
GGTTTGACCGGTCGTAAAATCATCGTGGACACTTATGGTGGTCACGGTGCTCACGGTGGTGGCGCGTTCTCTGGTAAAGA
TCCTTCCAAAGTGGACCGCTCTGCCGCTTACGCATCACGTCATATCGCGAAGAACATCGTGGGAGCGGGTCTTGCGGAAC
GCTGCCTGGTTCAGGTGGCTTACGCGATCGGTGTGGCTGAGCCTGTCAGCATCACTGTGAATGACTACGGAACAAGCAAA
GTTGGCCCGGAAGTGCTTGAAAAAGCGGTCCGCCAGGTCTTCGATTTGCGCCCAGCTCGCATTACAAAGGATCTGGACCT
GCTAAGACCGATTTACAGCCCGACAGCGGCTTACGGCCACTTTGGTCGTAACGAAGAGTCCTTCACTTGGGAGCGCCTGA
ACAAGGTCGACCAGCTGAAAGACGCTGTAAAGACTTTGGCTTAA

Upstream 100 bases:

>100_bases
TGGCCGCTCGCTAAATTCCAGCTCAATTTGACTGTATTTTAGGATGGTCCCAGAGGGGGCCGAGCTCATTAACACCATTC
CCTAATGAGGAACGAACGCA

Downstream 100 bases:

>100_bases
ATTTTTATAAAACCTTAATCATTGCGCCGAGTTGTCACTAGCAACTCGGCGCTTCTTATTTATACTACCGCCCATGGATC
CTAAATACATACGCAATTTC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MKNYLFTSESVSEGHPDKMADQISDGILDAILAQDPKGRVACETLLTTGLVVVAGEITTSAKVNFSEVARDVVKRIGYDH
SDKGFDYKTCGVMIAVGQQSPDIAVGVKETLSDNQGAGDQGLMFGYAVNETPELMPLSIAMSHKLVKDLAALRKANKVDW
LRPDAKSQVTVQYENGIAKRIDAVVISTQHADSVSNSTIQEFITEELIKKSIPGNWIDSKTKFFINPTGRFVTGGPMGDA
GLTGRKIIVDTYGGHGAHGGGAFSGKDPSKVDRSAAYASRHIAKNIVGAGLAERCLVQVAYAIGVAEPVSITVNDYGTSK
VGPEVLEKAVRQVFDLRPARITKDLDLLRPIYSPTAAYGHFGRNEESFTWERLNKVDQLKDAVKTLA

Sequences:

>Translated_387_residues
MKNYLFTSESVSEGHPDKMADQISDGILDAILAQDPKGRVACETLLTTGLVVVAGEITTSAKVNFSEVARDVVKRIGYDH
SDKGFDYKTCGVMIAVGQQSPDIAVGVKETLSDNQGAGDQGLMFGYAVNETPELMPLSIAMSHKLVKDLAALRKANKVDW
LRPDAKSQVTVQYENGIAKRIDAVVISTQHADSVSNSTIQEFITEELIKKSIPGNWIDSKTKFFINPTGRFVTGGPMGDA
GLTGRKIIVDTYGGHGAHGGGAFSGKDPSKVDRSAAYASRHIAKNIVGAGLAERCLVQVAYAIGVAEPVSITVNDYGTSK
VGPEVLEKAVRQVFDLRPARITKDLDLLRPIYSPTAAYGHFGRNEESFTWERLNKVDQLKDAVKTLA
>Mature_387_residues
MKNYLFTSESVSEGHPDKMADQISDGILDAILAQDPKGRVACETLLTTGLVVVAGEITTSAKVNFSEVARDVVKRIGYDH
SDKGFDYKTCGVMIAVGQQSPDIAVGVKETLSDNQGAGDQGLMFGYAVNETPELMPLSIAMSHKLVKDLAALRKANKVDW
LRPDAKSQVTVQYENGIAKRIDAVVISTQHADSVSNSTIQEFITEELIKKSIPGNWIDSKTKFFINPTGRFVTGGPMGDA
GLTGRKIIVDTYGGHGAHGGGAFSGKDPSKVDRSAAYASRHIAKNIVGAGLAERCLVQVAYAIGVAEPVSITVNDYGTSK
VGPEVLEKAVRQVFDLRPARITKDLDLLRPIYSPTAAYGHFGRNEESFTWERLNKVDQLKDAVKTLA

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=374, Percent_Identity=57.4866310160428, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI4557737, Length=373, Percent_Identity=57.372654155496, Blast_Score=416, Evalue=1e-116,
Organism=Escherichia coli, GI1789311, Length=382, Percent_Identity=63.8743455497382, Blast_Score=503, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17538498, Length=374, Percent_Identity=59.6256684491979, Blast_Score=439, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17538494, Length=374, Percent_Identity=59.6256684491979, Blast_Score=439, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI25145633, Length=384, Percent_Identity=58.59375, Blast_Score=432, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17551082, Length=375, Percent_Identity=56, Blast_Score=411, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI32565905, Length=374, Percent_Identity=54.5454545454545, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI32565911, Length=328, Percent_Identity=57.3170731707317, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25145635, Length=338, Percent_Identity=56.2130177514793, Blast_Score=357, Evalue=5e-99,
Organism=Caenorhabditis elegans, GI32565903, Length=301, Percent_Identity=58.1395348837209, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI32565909, Length=82, Percent_Identity=62.1951219512195, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32565907, Length=39, Percent_Identity=79.4871794871795, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320710, Length=381, Percent_Identity=56.1679790026247, Blast_Score=432, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6323209, Length=375, Percent_Identity=56, Blast_Score=424, Evalue=1e-119,
Organism=Drosophila melanogaster, GI45552159, Length=373, Percent_Identity=57.372654155496, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580529, Length=373, Percent_Identity=57.372654155496, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580537, Length=373, Percent_Identity=57.372654155496, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580533, Length=373, Percent_Identity=57.372654155496, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580535, Length=373, Percent_Identity=57.372654155496, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580531, Length=373, Percent_Identity=57.372654155496, Blast_Score=419, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24580527, Length=373, Percent_Identity=56.8364611260054, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580525, Length=373, Percent_Identity=56.8364611260054, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580541, Length=374, Percent_Identity=54.5454545454545, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580539, Length=118, Percent_Identity=57.6271186440678, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_BDEBA (Q6MPK2)

Other databases:

- EMBL:   BX842648
- RefSeq:   NP_967802.1
- ProteinModelPortal:   Q6MPK2
- SMR:   Q6MPK2
- GeneID:   2733921
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd0850
- NMPDR:   fig|264462.1.peg.772
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- PhylomeDB:   Q6MPK2
- ProtClustDB:   PRK05250
- BioCyc:   BBAC264462:BD0850-MONOMER
- BRENDA:   2.5.1.6
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 41703; Mature: 41703

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNYLFTSESVSEGHPDKMADQISDGILDAILAQDPKGRVACETLLTTGLVVVAGEITTS
CCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCEEEEEECCCCC
AKVNFSEVARDVVKRIGYDHSDKGFDYKTCGVMIAVGQQSPDIAVGVKETLSDNQGAGDQ
CCCCHHHHHHHHHHHCCCCCCCCCCCEEECEEEEEECCCCCCEEECHHHHHCCCCCCCCC
GLMFGYAVNETPELMPLSIAMSHKLVKDLAALRKANKVDWLRPDAKSQVTVQYENGIAKR
CEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHH
IDAVVISTQHADSVSNSTIQEFITEELIKKSIPGNWIDSKTKFFINPTGRFVTGGPMGDA
HHEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGHGAHGGGAFSGKDPSKVDRSAAYASRHIAKNIVGAGLAERCLVQVA
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
YAIGVAEPVSITVNDYGTSKVGPEVLEKAVRQVFDLRPARITKDLDLLRPIYSPTAAYGH
HHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHC
FGRNEESFTWERLNKVDQLKDAVKTLA
CCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNYLFTSESVSEGHPDKMADQISDGILDAILAQDPKGRVACETLLTTGLVVVAGEITTS
CCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCEEEEEECCCCC
AKVNFSEVARDVVKRIGYDHSDKGFDYKTCGVMIAVGQQSPDIAVGVKETLSDNQGAGDQ
CCCCHHHHHHHHHHHCCCCCCCCCCCEEECEEEEEECCCCCCEEECHHHHHCCCCCCCCC
GLMFGYAVNETPELMPLSIAMSHKLVKDLAALRKANKVDWLRPDAKSQVTVQYENGIAKR
CEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHH
IDAVVISTQHADSVSNSTIQEFITEELIKKSIPGNWIDSKTKFFINPTGRFVTGGPMGDA
HHEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCEEECCCCCCC
GLTGRKIIVDTYGGHGAHGGGAFSGKDPSKVDRSAAYASRHIAKNIVGAGLAERCLVQVA
CCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
YAIGVAEPVSITVNDYGTSKVGPEVLEKAVRQVFDLRPARITKDLDLLRPIYSPTAAYGH
HHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHC
FGRNEESFTWERLNKVDQLKDAVKTLA
CCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA