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The map label for this gene is lepA [H]

Identifier: 42522423

GI number: 42522423

Start: 797125

End: 798966

Strand: Direct

Name: lepA [H]

Synonym: Bd0851

Alternate gene names: 42522423

Gene position: 797125-798966 (Clockwise)

Preceding gene: 42522422

Following gene: 42522424

Centisome position: 21.07

GC content: 49.46

Gene sequence:

>1842_bases
TTGTCACTAGCAACTCGGCGCTTCTTATTTATACTACCGCCCATGGATCCTAAATACATACGCAATTTCTCTATTATCGC
TCACATCGACCACGGCAAATCTACTTTGGCTGACGGTCTTCTTTCCGCCACCGGCTCATTGTCGGATCGTGAGAAAAAGG
ATCAGTTCCTCGACAACATGGAACTTGAGCGCGAACGCGGCATCACCATCAAAGCTCAAACAGTCTGTCTGGATTTCAAA
TCCAAAGACGGCAACATGTATCAGATCAACTTGATCGATACACCGGGGCACGTGGACTTCTCTTACGAAGTGTCCCGTTC
TTTGGCCGCTTGTGAAGGCGCGATTCTTGTCGTTGACGCCGCTCAAGGGGTGGAAGCACAAACCCTGGCGAACGTTTATC
TGGCCATGGAAAATAATCTTGAGATCATTCCTGTTCTGAACAAGATTGATCTTCCGTCCGCAGATCCAGAAGGTGTTGCG
AAGCAGATCGAAGACACTGTCGGCTTGGATTGCACAGGGATCATTCACGCGTCGGCGAAAGAAAAGATCGGTATCACCGA
TATTCTGGAAGCGATCGTTGAAAAAGTTCCGCCACCAAAAGCGGACCGCAGCCTGACACCAAGAGCTCTTATTTTCGACT
CTTGGTTTGATGCTTATCAGGGCGTTGTTGTCCTGGTTCGTATGGTCGACGGCGTGATCAAAAAAGGTGACAAGATCAAG
TTCATGGCCACCGATCGTGACTATGAAGTTCTGCGCATGGGTAAGTACAAACCATTCCCGTTAATGCAGGATGTTCTTGA
GGCGGGTGAAGTGGGCTTCATCGTCTGTGGTATTAAAGATATCCGTGACGTTCAGGTGGGTGACACCGTCACTTCCGCAA
AACACCCGGCGACCCAGCCTTTGGCAGGTTTCCAGCGTATCAAGCCGATGGTTTTTGCCGGTATCTTCCCGGTCGTGGCT
TCTGAATACGAAAACCTGAAGGACGCTTTGGACAAACTTTGTCTGAATGACTCGTCTTTGACGTTCGAGGTTGAAAAATC
CGCGGCACTTGGTTTCGGATACCGTTGCGGTTTCCTGGGGCTGTTGCACATGGAAATCGTTCAAGAGCGTCTTGAGCGAG
AATTCAATCTGGATCTGATCACCACGGCGCCGACGGTTGTTTACCGTATCACAAAAACAGACGGCACCGAGATGATGCTG
GAAAATCCGTCCGGTATGCCGGTGGAAACCCAGATTGCCAAGTTTGAAGAGCCGTATGTAAAAGTGACCCTGCACACGCC
GACTGATTATATCGGCGGCATTCTGAAGCTGTGCGAGGACAAGCGTGGTATCCAGCAGAAGATGGAATACGTCACCGACA
AAAAAGTCATCATTGAGTACAAGTTGCCGATGAATGAAATGGTGATGGACTTCTACGATCGTTTGAAATCCATCTCCAAA
GGTTATGCTTCTTTGGAGTATGAATTCGTCGGTTTTGAGGAATCAGACCTGGTGAAGCTGGATATTCTGATTAACGGTGA
GGCTATCGATGCCCTGTCTTTGATCGTTCACCGATCCAAGGCGCAAAACCGTGGCCGCTTGCTGGCTGAAAAAATGAAAG
AACTGATTCCTCGTCAGATGTATCAGGTGGCGATCCAGGCAGCTATCGGTGCCAAGATCATCGCGCGTGAGACTCTGGGG
GCAATCAGAAAAGACGTGACCGCCAAGTGTTATGGTGGTGATATTTCCCGTAAGCGTAAGCTTCTGGAAAAGCAAAAAGA
GGGTAAGAAGCGCATGAAGGCCATCGGCCATGTCGAAGTCCCTCAAGAAGCCTTCCTCGCGATCCTGAAAGTCGAAGACT
AG

Upstream 100 bases:

>100_bases
GAGTCCTTCACTTGGGAGCGCCTGAACAAGGTCGACCAGCTGAAAGACGCTGTAAAGACTTTGGCTTAAATTTTTATAAA
ACCTTAATCATTGCGCCGAG

Downstream 100 bases:

>100_bases
GAGAATCAACATGAGCGACAAACAACCAAAAAGCTGGGACTTTAGAACGAAACACTTCTGGACAGAGGGTTGGGGCTCTT
TGTTCCTGGCGGTGTTCATC

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MSLATRRFLFILPPMDPKYIRNFSIIAHIDHGKSTLADGLLSATGSLSDREKKDQFLDNMELERERGITIKAQTVCLDFK
SKDGNMYQINLIDTPGHVDFSYEVSRSLAACEGAILVVDAAQGVEAQTLANVYLAMENNLEIIPVLNKIDLPSADPEGVA
KQIEDTVGLDCTGIIHASAKEKIGITDILEAIVEKVPPPKADRSLTPRALIFDSWFDAYQGVVVLVRMVDGVIKKGDKIK
FMATDRDYEVLRMGKYKPFPLMQDVLEAGEVGFIVCGIKDIRDVQVGDTVTSAKHPATQPLAGFQRIKPMVFAGIFPVVA
SEYENLKDALDKLCLNDSSLTFEVEKSAALGFGYRCGFLGLLHMEIVQERLEREFNLDLITTAPTVVYRITKTDGTEMML
ENPSGMPVETQIAKFEEPYVKVTLHTPTDYIGGILKLCEDKRGIQQKMEYVTDKKVIIEYKLPMNEMVMDFYDRLKSISK
GYASLEYEFVGFEESDLVKLDILINGEAIDALSLIVHRSKAQNRGRLLAEKMKELIPRQMYQVAIQAAIGAKIIARETLG
AIRKDVTAKCYGGDISRKRKLLEKQKEGKKRMKAIGHVEVPQEAFLAILKVED

Sequences:

>Translated_613_residues
MSLATRRFLFILPPMDPKYIRNFSIIAHIDHGKSTLADGLLSATGSLSDREKKDQFLDNMELERERGITIKAQTVCLDFK
SKDGNMYQINLIDTPGHVDFSYEVSRSLAACEGAILVVDAAQGVEAQTLANVYLAMENNLEIIPVLNKIDLPSADPEGVA
KQIEDTVGLDCTGIIHASAKEKIGITDILEAIVEKVPPPKADRSLTPRALIFDSWFDAYQGVVVLVRMVDGVIKKGDKIK
FMATDRDYEVLRMGKYKPFPLMQDVLEAGEVGFIVCGIKDIRDVQVGDTVTSAKHPATQPLAGFQRIKPMVFAGIFPVVA
SEYENLKDALDKLCLNDSSLTFEVEKSAALGFGYRCGFLGLLHMEIVQERLEREFNLDLITTAPTVVYRITKTDGTEMML
ENPSGMPVETQIAKFEEPYVKVTLHTPTDYIGGILKLCEDKRGIQQKMEYVTDKKVIIEYKLPMNEMVMDFYDRLKSISK
GYASLEYEFVGFEESDLVKLDILINGEAIDALSLIVHRSKAQNRGRLLAEKMKELIPRQMYQVAIQAAIGAKIIARETLG
AIRKDVTAKCYGGDISRKRKLLEKQKEGKKRMKAIGHVEVPQEAFLAILKVED
>Mature_612_residues
SLATRRFLFILPPMDPKYIRNFSIIAHIDHGKSTLADGLLSATGSLSDREKKDQFLDNMELERERGITIKAQTVCLDFKS
KDGNMYQINLIDTPGHVDFSYEVSRSLAACEGAILVVDAAQGVEAQTLANVYLAMENNLEIIPVLNKIDLPSADPEGVAK
QIEDTVGLDCTGIIHASAKEKIGITDILEAIVEKVPPPKADRSLTPRALIFDSWFDAYQGVVVLVRMVDGVIKKGDKIKF
MATDRDYEVLRMGKYKPFPLMQDVLEAGEVGFIVCGIKDIRDVQVGDTVTSAKHPATQPLAGFQRIKPMVFAGIFPVVAS
EYENLKDALDKLCLNDSSLTFEVEKSAALGFGYRCGFLGLLHMEIVQERLEREFNLDLITTAPTVVYRITKTDGTEMMLE
NPSGMPVETQIAKFEEPYVKVTLHTPTDYIGGILKLCEDKRGIQQKMEYVTDKKVIIEYKLPMNEMVMDFYDRLKSISKG
YASLEYEFVGFEESDLVKLDILINGEAIDALSLIVHRSKAQNRGRLLAEKMKELIPRQMYQVAIQAAIGAKIIARETLGA
IRKDVTAKCYGGDISRKRKLLEKQKEGKKRMKAIGHVEVPQEAFLAILKVED

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=601, Percent_Identity=50.5823627287854, Blast_Score=632, Evalue=0.0,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=48.8721804511278, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=42.9530201342282, Blast_Score=109, Evalue=8e-24,
Organism=Homo sapiens, GI25306283, Length=143, Percent_Identity=47.5524475524476, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI25306287, Length=144, Percent_Identity=47.2222222222222, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI19923640, Length=144, Percent_Identity=47.2222222222222, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI18390331, Length=150, Percent_Identity=39.3333333333333, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI310132016, Length=109, Percent_Identity=45.8715596330275, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI310110807, Length=109, Percent_Identity=45.8715596330275, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI310123363, Length=109, Percent_Identity=45.8715596330275, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI217272894, Length=159, Percent_Identity=34.5911949685535, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI217272892, Length=159, Percent_Identity=34.5911949685535, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI94966752, Length=83, Percent_Identity=46.9879518072289, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788922, Length=594, Percent_Identity=60.4377104377104, Blast_Score=725, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=504, Percent_Identity=26.984126984127, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1789738, Length=177, Percent_Identity=35.0282485875706, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1790835, Length=164, Percent_Identity=32.9268292682927, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1789559, Length=282, Percent_Identity=27.3049645390071, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1789737, Length=133, Percent_Identity=33.0827067669173, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI1790412, Length=133, Percent_Identity=33.0827067669173, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=621, Percent_Identity=41.0628019323672, Blast_Score=493, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=41.044776119403, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17556745, Length=159, Percent_Identity=35.2201257861635, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=41.044776119403, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=157, Percent_Identity=38.2165605095541, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=153, Percent_Identity=38.562091503268, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17552882, Length=186, Percent_Identity=32.7956989247312, Blast_Score=91, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=287, Percent_Identity=27.5261324041812, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71994658, Length=133, Percent_Identity=27.8195488721804, Blast_Score=67, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI25141371, Length=333, Percent_Identity=26.7267267267267, Blast_Score=67, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=598, Percent_Identity=46.6555183946488, Blast_Score=545, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6324707, Length=143, Percent_Identity=43.3566433566434, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=143, Percent_Identity=43.3566433566434, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323098, Length=220, Percent_Identity=33.1818181818182, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324166, Length=145, Percent_Identity=42.0689655172414, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6322359, Length=112, Percent_Identity=42.8571428571429, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6324761, Length=251, Percent_Identity=28.2868525896414, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322675, Length=145, Percent_Identity=31.0344827586207, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI78706572, Length=604, Percent_Identity=46.3576158940397, Blast_Score=575, Evalue=1e-164,
Organism=Drosophila melanogaster, GI28574573, Length=217, Percent_Identity=39.63133640553, Blast_Score=112, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24582462, Length=150, Percent_Identity=40.6666666666667, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=39.8692810457516, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=39.8692810457516, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=39.8692810457516, Blast_Score=102, Evalue=1e-21,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=39.5973154362416, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21357743, Length=153, Percent_Identity=34.640522875817, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI28572034, Length=237, Percent_Identity=29.1139240506329, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281363316, Length=283, Percent_Identity=27.9151943462898, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17864358, Length=283, Percent_Identity=27.9151943462898, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 68460; Mature: 68329

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLATRRFLFILPPMDPKYIRNFSIIAHIDHGKSTLADGLLSATGSLSDREKKDQFLDNM
CCCCCCEEEEEECCCCHHHHCCEEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHCC
ELERERGITIKAQTVCLDFKSKDGNMYQINLIDTPGHVDFSYEVSRSLAACEGAILVVDA
HHHHHCCCEEEEEEEEEEEECCCCCEEEEEEECCCCCCEEEHHHHHHHHHCCCEEEEEEC
AQGVEAQTLANVYLAMENNLEIIPVLNKIDLPSADPEGVAKQIEDTVGLDCTGIIHASAK
CCCCCHHHHHEEEEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCC
EKIGITDILEAIVEKVPPPKADRSLTPRALIFDSWFDAYQGVVVLVRMVDGVIKKGDKIK
CCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
FMATDRDYEVLRMGKYKPFPLMQDVLEAGEVGFIVCGIKDIRDVQVGDTVTSAKHPATQP
EEECCCCCHHHCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCEECCCCCCCCCCCCCCC
LAGFQRIKPMVFAGIFPVVASEYENLKDALDKLCLNDSSLTFEVEKSAALGFGYRCGFLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHH
LLHMEIVQERLEREFNLDLITTAPTVVYRITKTDGTEMMLENPSGMPVETQIAKFEEPYV
HHHHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCCEEEEECCCCCCCHHHHHHCCCCEE
KVTLHTPTDYIGGILKLCEDKRGIQQKMEYVTDKKVIIEYKLPMNEMVMDFYDRLKSISK
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHC
GYASLEYEFVGFEESDLVKLDILINGEAIDALSLIVHRSKAQNRGRLLAEKMKELIPRQM
CCCEEEEEEECCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQVAIQAAIGAKIIARETLGAIRKDVTAKCYGGDISRKRKLLEKQKEGKKRMKAIGHVEV
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
PQEAFLAILKVED
CHHHEEEEEEECC
>Mature Secondary Structure 
SLATRRFLFILPPMDPKYIRNFSIIAHIDHGKSTLADGLLSATGSLSDREKKDQFLDNM
CCCCCEEEEEECCCCHHHHCCEEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHCC
ELERERGITIKAQTVCLDFKSKDGNMYQINLIDTPGHVDFSYEVSRSLAACEGAILVVDA
HHHHHCCCEEEEEEEEEEEECCCCCEEEEEEECCCCCCEEEHHHHHHHHHCCCEEEEEEC
AQGVEAQTLANVYLAMENNLEIIPVLNKIDLPSADPEGVAKQIEDTVGLDCTGIIHASAK
CCCCCHHHHHEEEEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCC
EKIGITDILEAIVEKVPPPKADRSLTPRALIFDSWFDAYQGVVVLVRMVDGVIKKGDKIK
CCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
FMATDRDYEVLRMGKYKPFPLMQDVLEAGEVGFIVCGIKDIRDVQVGDTVTSAKHPATQP
EEECCCCCHHHCCCCCCCCHHHHHHHCCCCCEEEEECCCCCCCEECCCCCCCCCCCCCCC
LAGFQRIKPMVFAGIFPVVASEYENLKDALDKLCLNDSSLTFEVEKSAALGFGYRCGFLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHH
LLHMEIVQERLEREFNLDLITTAPTVVYRITKTDGTEMMLENPSGMPVETQIAKFEEPYV
HHHHHHHHHHHHHHCCCEEEECCCEEEEEEECCCCCEEEEECCCCCCCHHHHHHCCCCEE
KVTLHTPTDYIGGILKLCEDKRGIQQKMEYVTDKKVIIEYKLPMNEMVMDFYDRLKSISK
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHC
GYASLEYEFVGFEESDLVKLDILINGEAIDALSLIVHRSKAQNRGRLLAEKMKELIPRQM
CCCEEEEEEECCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQVAIQAAIGAKIIARETLGAIRKDVTAKCYGGDISRKRKLLEKQKEGKKRMKAIGHVEV
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
PQEAFLAILKVED
CHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA