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The map label for this gene is pcbC [H]

Identifier: 42522061

GI number: 42522061

Start: 424920

End: 425903

Strand: Direct

Name: pcbC [H]

Synonym: Bd0452

Alternate gene names: 42522061

Gene position: 424920-425903 (Clockwise)

Preceding gene: 42522060

Following gene: 42522062

Centisome position: 11.23

GC content: 48.78

Gene sequence:

>984_bases
GTGAGAACACTGGAAACTGCTTCTCATTCTGAAAACAGCACTCTTCGTAAAGTGCCAACATTGAGCCTTGCAAGTTACAC
CAAAGGGACTGCCGGCGAACGCTCAGAGTTCATCGGCAAACTTTTCACTGGTCTGAAAGAATATGGTTTCATCATTCTGA
AAGATCACAACGTCCAAGCCGCAGATCTGCACAAAGCTTACGACATCCTTAAAAACTTCTACGCACTTCCGACAGAAGTT
AAAAAATCCTATATCTCTCCAAAAGCCGGCTTCCAGCGCGGTTACACTCCATTCGGTCAGGAACATGCCAAAGATTCCCC
GGTGATGGACTTGAAAGAGTTCTGGCACGTGGGCCGTGAGCTTTCTGAAGGCAACCCCTTGAAGGCTGTTTACCCTGCAA
ACGTATGGCCGTCTGAATTGCCGGAATTCCAGTCCCATTTCTCCAAGTTGTATGCAGCTTTGGAAGAAGCGGGCGATGTG
ATGCTGGAAGCGTTGACCATGCCTTTGGAAGTTGAAAAAGATTTCTTTGCCAAGATGACCAAAGATGGAAACTCCATCCT
GAGACTTTTGCACTACCCACCAATTCCTGAGGGTGTGGATCCTCGTTGTGTTCGCGCGGCGGCTCACGAAGACATCAACT
TCATTACAATTCTTCCAGCTGCAACCGCTTCCGGTCTGCAATTGAAAGATCGTGATGGCACTTGGTTGGATATCGACTCT
GAGCCAGACACTTTGATCGTGGACGTGGGCGACATGCTGGCTCGTCTGACGAATGACGTTTTGCCAAGCACTACCCACCG
TGTGATCAATCCACAGGATGGTACAAATCAAAGCCGTTATTCCATGCCGTTCTTCATGCACCCGCATCCGGAGGCAATGC
TAAGCTGCTTGCCATCTTGTAAAGGGACAGGTGCGAAGTACGCTGATATTACTGGACAGGATTTCTTGATGCAGCGACTG
CGCGAGATTGGTCTGTTAAAGTAA

Upstream 100 bases:

>100_bases
GTGCGCGTCTTCGATATTCTTTTTATGCATAATTAGTTGCAGTTGCCTTTTAATTGCGCAGCAGGCTACTCTCCGGATGA
ACCAATACAAGGGAGGATTC

Downstream 100 bases:

>100_bases
GCCCAAACTTGTCACCACAGTTGAAATGACTTCAAGAAAGGGATCCACACGGATCCCTTTCTTGCGTTTTGGGCCCTGGG
GCCACTAGAACAAAGTCACC

Product: oxidoreductase

Products: NA

Alternate protein names: IPNS [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MRTLETASHSENSTLRKVPTLSLASYTKGTAGERSEFIGKLFTGLKEYGFIILKDHNVQAADLHKAYDILKNFYALPTEV
KKSYISPKAGFQRGYTPFGQEHAKDSPVMDLKEFWHVGRELSEGNPLKAVYPANVWPSELPEFQSHFSKLYAALEEAGDV
MLEALTMPLEVEKDFFAKMTKDGNSILRLLHYPPIPEGVDPRCVRAAAHEDINFITILPAATASGLQLKDRDGTWLDIDS
EPDTLIVDVGDMLARLTNDVLPSTTHRVINPQDGTNQSRYSMPFFMHPHPEAMLSCLPSCKGTGAKYADITGQDFLMQRL
REIGLLK

Sequences:

>Translated_327_residues
MRTLETASHSENSTLRKVPTLSLASYTKGTAGERSEFIGKLFTGLKEYGFIILKDHNVQAADLHKAYDILKNFYALPTEV
KKSYISPKAGFQRGYTPFGQEHAKDSPVMDLKEFWHVGRELSEGNPLKAVYPANVWPSELPEFQSHFSKLYAALEEAGDV
MLEALTMPLEVEKDFFAKMTKDGNSILRLLHYPPIPEGVDPRCVRAAAHEDINFITILPAATASGLQLKDRDGTWLDIDS
EPDTLIVDVGDMLARLTNDVLPSTTHRVINPQDGTNQSRYSMPFFMHPHPEAMLSCLPSCKGTGAKYADITGQDFLMQRL
REIGLLK
>Mature_327_residues
MRTLETASHSENSTLRKVPTLSLASYTKGTAGERSEFIGKLFTGLKEYGFIILKDHNVQAADLHKAYDILKNFYALPTEV
KKSYISPKAGFQRGYTPFGQEHAKDSPVMDLKEFWHVGRELSEGNPLKAVYPANVWPSELPEFQSHFSKLYAALEEAGDV
MLEALTMPLEVEKDFFAKMTKDGNSILRLLHYPPIPEGVDPRCVRAAAHEDINFITILPAATASGLQLKDRDGTWLDIDS
EPDTLIVDVGDMLARLTNDVLPSTTHRVINPQDGTNQSRYSMPFFMHPHPEAMLSCLPSCKGTGAKYADITGQDFLMQRL
REIGLLK

Specific function: Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin [H]

COG id: COG3491

COG function: function code R; Isopenicillin N synthase and related dioxygenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Drosophila melanogaster, GI85816264, Length=302, Percent_Identity=27.1523178807947, Blast_Score=95, Evalue=7e-20,
Organism=Drosophila melanogaster, GI28572124, Length=291, Percent_Identity=25.085910652921, Blast_Score=85, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002057
- InterPro:   IPR002283
- InterPro:   IPR005123 [H]

Pfam domain/function: PF03171 2OG-FeII_Oxy [H]

EC number: =1.21.3.1 [H]

Molecular weight: Translated: 36532; Mature: 36532

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTLETASHSENSTLRKVPTLSLASYTKGTAGERSEFIGKLFTGLKEYGFIILKDHNVQA
CCCCCCCCCCCCCHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCH
ADLHKAYDILKNFYALPTEVKKSYISPKAGFQRGYTPFGQEHAKDSPVMDLKEFWHVGRE
HHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHHH
LSEGNPLKAVYPANVWPSELPEFQSHFSKLYAALEEAGDVMLEALTMPLEVEKDFFAKMT
HCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
KDGNSILRLLHYPPIPEGVDPRCVRAAAHEDINFITILPAATASGLQLKDRDGTWLDIDS
CCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCEEEECC
EPDTLIVDVGDMLARLTNDVLPSTTHRVINPQDGTNQSRYSMPFFMHPHPEAMLSCLPSC
CCCEEEEEHHHHHHHHHHHCCCCCHHCEECCCCCCCCCCCCCCEEECCCHHHHHHHHCCC
KGTGAKYADITGQDFLMQRLREIGLLK
CCCCCEEECCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRTLETASHSENSTLRKVPTLSLASYTKGTAGERSEFIGKLFTGLKEYGFIILKDHNVQA
CCCCCCCCCCCCCHHHHCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCH
ADLHKAYDILKNFYALPTEVKKSYISPKAGFQRGYTPFGQEHAKDSPVMDLKEFWHVGRE
HHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHCCCCCCCHHHCCCCCCHHHHHHHHHHHH
LSEGNPLKAVYPANVWPSELPEFQSHFSKLYAALEEAGDVMLEALTMPLEVEKDFFAKMT
HCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
KDGNSILRLLHYPPIPEGVDPRCVRAAAHEDINFITILPAATASGLQLKDRDGTWLDIDS
CCHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCEEEECC
EPDTLIVDVGDMLARLTNDVLPSTTHRVINPQDGTNQSRYSMPFFMHPHPEAMLSCLPSC
CCCEEEEEHHHHHHHHHHHCCCCCHHCEECCCCCCCCCCCCCCEEECCCHHHHHHHHCCC
KGTGAKYADITGQDFLMQRLREIGLLK
CCCCCEEECCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3130293 [H]