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The map label for this gene is strB [H]

Identifier: 42521930

GI number: 42521930

Start: 296463

End: 297362

Strand: Direct

Name: strB [H]

Synonym: Bd0307

Alternate gene names: 42521930

Gene position: 296463-297362 (Clockwise)

Preceding gene: 42521928

Following gene: 42521931

Centisome position: 7.84

GC content: 53.33

Gene sequence:

>900_bases
ATGAATTTTCCCGCACCGTTCGTGAAAAACATCCGCGACACTTTTGGCATGGCCGGTGAGGAGTGGCTGCAAGGTCTTCC
GTCATTGCTGCGAGGTCTTTGTCTGCGCTGGGATCTGACGATCACCGGTCGCGCTGAAGATCTGTCTTATAACTTTGTGG
GATATGCACGCACCAATGCGGATAAAAAACCTGTGGTCCTCAAAGTACTTTGCCCGGATGGGATTTTGGATAAAGAACTG
CTATGGCTGAAGTCTTATCCCGAAGTCACCCCCGCGGTTTTGGCGGTGGAGGCCGAGCAGCGGGCTTTTATGATGCAGAT
GGTTTTGCCGGGGAATTCTTTAAAGTCACTGGTGAAACAAGGCAAGGACGCTGAGGCCACGGCGGTGATTGCGCAAATGA
TCCGGGCTTTGGGAAAAGAGCGTCCTGCAGGAGTGCTCCCTTATAAGCATGTGCGTGACTTCTTGCCGACACTGTCGCTG
CTGCATGGGAAACTTCCTTCCGAACTGGTGCTGCTGGTGGCGGTTCTTTATGACGAGCTCTGCATGACGGCTCCGCGGGA
CGTGCTTTTGCACGGCGATCTTCACCATGACAATATCCTGGCTTCGGGGGATGGGTGGAAGATCATTGATCCTCACGGAT
ACTTGGGCGACCCGGCCTTTGAAGTCGGGGCAATGATTTCAAACCCTCTGGACTGTTATCCATCGGGACCAGCACTGGAA
AAAATTCTGGAGCAGAGACTGAACATCCTTTACGGTGAGCTTGCCATGGATCCTTATCGGATTCTGGGCTGGGCGTTTTG
TTATTCCATGCTTTCTGCAGCCTGGAGCGTGGAGGACCGGCAGGAGGTCCCGGAGAACCTCTTGCAAATCGTGCGGATTT
TAGCCAGCAAACTGACATGA

Upstream 100 bases:

>100_bases
CGCGTTCCTGTCTTAAGTCAAAAGCCCCTCCGCCAATCTGGATCAAGGTCCAAAAGGTACCTGCTTACTTTTTCCGAAGC
GCTGTGCCAGAGTCAATAGT

Downstream 100 bases:

>100_bases
TTGAGAGATGCTGTTGCAGGACCACTGACAGAAAACAGCCGGACAAAGCGCATTCTTCTTTCTAGAATAAAAAAAGGGGG
GGCGTTTATGTTGAATGAAA

Product: streptomycin resistance protein

Products: NA

Alternate protein names: Streptomycin 3''-phosphotransferase; SPH [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MNFPAPFVKNIRDTFGMAGEEWLQGLPSLLRGLCLRWDLTITGRAEDLSYNFVGYARTNADKKPVVLKVLCPDGILDKEL
LWLKSYPEVTPAVLAVEAEQRAFMMQMVLPGNSLKSLVKQGKDAEATAVIAQMIRALGKERPAGVLPYKHVRDFLPTLSL
LHGKLPSELVLLVAVLYDELCMTAPRDVLLHGDLHHDNILASGDGWKIIDPHGYLGDPAFEVGAMISNPLDCYPSGPALE
KILEQRLNILYGELAMDPYRILGWAFCYSMLSAAWSVEDRQEVPENLLQIVRILASKLT

Sequences:

>Translated_299_residues
MNFPAPFVKNIRDTFGMAGEEWLQGLPSLLRGLCLRWDLTITGRAEDLSYNFVGYARTNADKKPVVLKVLCPDGILDKEL
LWLKSYPEVTPAVLAVEAEQRAFMMQMVLPGNSLKSLVKQGKDAEATAVIAQMIRALGKERPAGVLPYKHVRDFLPTLSL
LHGKLPSELVLLVAVLYDELCMTAPRDVLLHGDLHHDNILASGDGWKIIDPHGYLGDPAFEVGAMISNPLDCYPSGPALE
KILEQRLNILYGELAMDPYRILGWAFCYSMLSAAWSVEDRQEVPENLLQIVRILASKLT
>Mature_299_residues
MNFPAPFVKNIRDTFGMAGEEWLQGLPSLLRGLCLRWDLTITGRAEDLSYNFVGYARTNADKKPVVLKVLCPDGILDKEL
LWLKSYPEVTPAVLAVEAEQRAFMMQMVLPGNSLKSLVKQGKDAEATAVIAQMIRALGKERPAGVLPYKHVRDFLPTLSL
LHGKLPSELVLLVAVLYDELCMTAPRDVLLHGDLHHDNILASGDGWKIIDPHGYLGDPAFEVGAMISNPLDCYPSGPALE
KILEQRLNILYGELAMDPYRILGWAFCYSMLSAAWSVEDRQEVPENLLQIVRILASKLT

Specific function: The aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation [H]

COG id: COG3570

COG function: function code V; Streptomycin 6-kinase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aminoglycoside phosphotransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006748 [H]

Pfam domain/function: PF04655 APH_6_hur [H]

EC number: =2.7.1.87 [H]

Molecular weight: Translated: 33254; Mature: 33254

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFPAPFVKNIRDTFGMAGEEWLQGLPSLLRGLCLRWDLTITGRAEDLSYNFVGYARTNA
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEEECCC
DKKPVVLKVLCPDGILDKELLWLKSYPEVTPAVLAVEAEQRAFMMQMVLPGNSLKSLVKQ
CCCCEEEEEECCCCCCCHHHHHHHCCCCCCHHHEEEEHHHHHHHHHHHCCCHHHHHHHHC
GKDAEATAVIAQMIRALGKERPAGVLPYKHVRDFLPTLSLLHGKLPSELVLLVAVLYDEL
CCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
CMTAPRDVLLHGDLHHDNILASGDGWKIIDPHGYLGDPAFEVGAMISNPLDCYPSGPALE
HHCCCCCEEEECCCCCCCEEECCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCCHHHH
KILEQRLNILYGELAMDPYRILGWAFCYSMLSAAWSVEDRQEVPENLLQIVRILASKLT
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNFPAPFVKNIRDTFGMAGEEWLQGLPSLLRGLCLRWDLTITGRAEDLSYNFVGYARTNA
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCEEEEEEEECCC
DKKPVVLKVLCPDGILDKELLWLKSYPEVTPAVLAVEAEQRAFMMQMVLPGNSLKSLVKQ
CCCCEEEEEECCCCCCCHHHHHHHCCCCCCHHHEEEEHHHHHHHHHHHCCCHHHHHHHHC
GKDAEATAVIAQMIRALGKERPAGVLPYKHVRDFLPTLSLLHGKLPSELVLLVAVLYDEL
CCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
CMTAPRDVLLHGDLHHDNILASGDGWKIIDPHGYLGDPAFEVGAMISNPLDCYPSGPALE
HHCCCCCEEEECCCCCCCEEECCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCCHHHH
KILEQRLNILYGELAMDPYRILGWAFCYSMLSAAWSVEDRQEVPENLLQIVRILASKLT
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3889831 [H]