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The map label for this gene is hemK [H]

Identifier: 42521716

GI number: 42521716

Start: 63609

End: 64490

Strand: Direct

Name: hemK [H]

Synonym: Bd0070

Alternate gene names: 42521716

Gene position: 63609-64490 (Clockwise)

Preceding gene: 42521715

Following gene: 42521717

Centisome position: 1.68

GC content: 50.11

Gene sequence:

>882_bases
ATGAAACTAAAAGAAATACTCGATAAAACCACAGCTTTCTTCAAAGACAAAAAAATCGACACTCCACGTTTGGATGCGGA
ACTGCTTTTGGCCCACGGGCTGAAGCTGGAACGCATTCAGCTTTATTTGCGCTTTGATCAGCCGATGAAGGATGAAGAGC
TGGCCGTTCTGCGGGAACTGGTACGTCGTCGTGCTTCCGGAGAGCCGGTGGCGTACATTATGGGTTATCGTGATTTCTTC
AATCACCGTTTCGAGGTGAACAACCAGGTGCTGATTCCGCGCCCGGAAACTGAACACATTGTGGAAGACGTTCTGGCGTG
GGCTTCGGACAAAGAAGCTTCCTTGGGTCTTATTGACCTGGGATGTGGTTCGGGGTGCATTGGATTAAGTCTGCTGAAGG
AATATCCGAACGCCAAACTGATCGCCGTGGACCTGCTTCCGGGAGCCATTGAGGTGGCTCAAAGAAATGCGCAGTCCCTG
GATGTGGCCGATCGGGTTCAATTCCTGAACTTGGATGCGGGGAATGTGGAAGCCATCATGTCGGCTTACAAGGACTTTAC
CGGTCAGAGCAGCATTGATGTGCTGGTTTCCAACCCTCCGTACATTGCTTCGGACGATCCGCAGGTGGAAGAAAACGTTA
AGAAGTTTGAACCCAACTCGGCTTTGTATGCCGAAGACAGTGGCCTGGCCCTACTAAAGGGATGGTCCAAAGCTTTCGCC
CCCTACCTGAAGACACCCGGCTTGATGTTGATGGAAATGGGAATGAGTCAGGGACCGGCGATGAAGCAGGCCTATGAAAG
CTTAAAAATATTTAACGAAATCAGTGTCATCAAAGATCTTTCTGGGCATGATAGAGTCATCCGCGGAGAAACACATGGAT
AA

Upstream 100 bases:

>100_bases
GCTGGATCAGGTTATGAGCGGTTCATTTGAGTTGCTGATTGATCCACTCATTGCTAACTTCCAGGCAGAGGCACTTAAAA
AACAAGTCTCTGCTTAATGA

Downstream 100 bases:

>100_bases
AATGGTCGTCATGGGCAATGGCCCACTAAAAGGTACTGTCGCTACGAGCGGCGCAAAGAACGCTGCTCTGCCAATTTTGT
TTTCGACTCTTCTTGCTGAG

Product: HemK protein

Products: NA

Alternate protein names: HemK protein homolog; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MKLKEILDKTTAFFKDKKIDTPRLDAELLLAHGLKLERIQLYLRFDQPMKDEELAVLRELVRRRASGEPVAYIMGYRDFF
NHRFEVNNQVLIPRPETEHIVEDVLAWASDKEASLGLIDLGCGSGCIGLSLLKEYPNAKLIAVDLLPGAIEVAQRNAQSL
DVADRVQFLNLDAGNVEAIMSAYKDFTGQSSIDVLVSNPPYIASDDPQVEENVKKFEPNSALYAEDSGLALLKGWSKAFA
PYLKTPGLMLMEMGMSQGPAMKQAYESLKIFNEISVIKDLSGHDRVIRGETHG

Sequences:

>Translated_293_residues
MKLKEILDKTTAFFKDKKIDTPRLDAELLLAHGLKLERIQLYLRFDQPMKDEELAVLRELVRRRASGEPVAYIMGYRDFF
NHRFEVNNQVLIPRPETEHIVEDVLAWASDKEASLGLIDLGCGSGCIGLSLLKEYPNAKLIAVDLLPGAIEVAQRNAQSL
DVADRVQFLNLDAGNVEAIMSAYKDFTGQSSIDVLVSNPPYIASDDPQVEENVKKFEPNSALYAEDSGLALLKGWSKAFA
PYLKTPGLMLMEMGMSQGPAMKQAYESLKIFNEISVIKDLSGHDRVIRGETHG
>Mature_293_residues
MKLKEILDKTTAFFKDKKIDTPRLDAELLLAHGLKLERIQLYLRFDQPMKDEELAVLRELVRRRASGEPVAYIMGYRDFF
NHRFEVNNQVLIPRPETEHIVEDVLAWASDKEASLGLIDLGCGSGCIGLSLLKEYPNAKLIAVDLLPGAIEVAQRNAQSL
DVADRVQFLNLDAGNVEAIMSAYKDFTGQSSIDVLVSNPPYIASDDPQVEENVKKFEPNSALYAEDSGLALLKGWSKAFA
PYLKTPGLMLMEMGMSQGPAMKQAYESLKIFNEISVIKDLSGHDRVIRGETHG

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=299, Percent_Identity=31.1036789297659, Blast_Score=122, Evalue=6e-28,
Organism=Escherichia coli, GI1787463, Length=270, Percent_Identity=34.4444444444444, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI87082085, Length=231, Percent_Identity=27.7056277056277, Blast_Score=89, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24582226, Length=251, Percent_Identity=25.4980079681275, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848
- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4; PF05175 MTS [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 32686; Mature: 32686

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKEILDKTTAFFKDKKIDTPRLDAELLLAHGLKLERIQLYLRFDQPMKDEELAVLREL
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHH
VRRRASGEPVAYIMGYRDFFNHRFEVNNQVLIPRPETEHIVEDVLAWASDKEASLGLIDL
HHHHCCCCCEEEEECCHHHHCCEEECCCEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEE
GCGSGCIGLSLLKEYPNAKLIAVDLLPGAIEVAQRNAQSLDVADRVQFLNLDAGNVEAIM
CCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHCCCCCCCHHHHHEEEECCCCCHHHHH
SAYKDFTGQSSIDVLVSNPPYIASDDPQVEENVKKFEPNSALYAEDSGLALLKGWSKAFA
HHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHH
PYLKTPGLMLMEMGMSQGPAMKQAYESLKIFNEISVIKDLSGHDRVIRGETHG
HHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCC
>Mature Secondary Structure
MKLKEILDKTTAFFKDKKIDTPRLDAELLLAHGLKLERIQLYLRFDQPMKDEELAVLREL
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCEEEEEEEEEECCCCCCHHHHHHHHH
VRRRASGEPVAYIMGYRDFFNHRFEVNNQVLIPRPETEHIVEDVLAWASDKEASLGLIDL
HHHHCCCCCEEEEECCHHHHCCEEECCCEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEE
GCGSGCIGLSLLKEYPNAKLIAVDLLPGAIEVAQRNAQSLDVADRVQFLNLDAGNVEAIM
CCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHCCCCCCCHHHHHEEEECCCCCHHHHH
SAYKDFTGQSSIDVLVSNPPYIASDDPQVEENVKKFEPNSALYAEDSGLALLKGWSKAFA
HHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHH
PYLKTPGLMLMEMGMSQGPAMKQAYESLKIFNEISVIKDLSGHDRVIRGETHG
HHHCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA