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The map label for this gene is prfA

Identifier: 42521715

GI number: 42521715

Start: 62532

End: 63605

Strand: Direct

Name: prfA

Synonym: Bd0069

Alternate gene names: 42521715

Gene position: 62532-63605 (Clockwise)

Preceding gene: 42521714

Following gene: 42521716

Centisome position: 1.65

GC content: 50.65

Gene sequence:

>1074_bases
ATGTTCTCGAAGTTAGATGCAGTCGAATCTCGTTACGAAGAAGTCAATATGGCTCTTCAAAGACCCGACATTGCCTCTAA
TCAGACGCAATACCGTGCTTTGATGAAAGAACTGGGCAATCTGGAAAAGATCGTTCTGGTTTATCGCGACTATCGCAAAA
AAACTGAAAATCTGAAAGCCAGCAAAGAGCTTCTGACCGCGGAACAGGATGCAGAAATGCGTGAGCTGATCCGTGAAGAG
GTGAAAGAGCTTGAGGCTGAGCTGCCTGATCTTGAACAACAGCTTAAAATCGCATTGATTCCCAAAGACCCGAACGATGA
CAAGAACACCATTCTTGAGATTCGTGCCGGAGCCGGCGGGGACGAGGCGGCTTTGTTCGCGGATGAATTGTTCCGTGGTT
ATGTTCACTATGCCTCCACTCAAGGGTGGAAGGTGGAAATGATTTCTTTCTCTGAAGGCAACGCCGGCGGAGCCAAAGAG
ATCATCGCCAGCATCACGGGCGATTCGGTCTTCAGCAAAATGAAGTATGAATCCGGCGTTCACCGTGTTCAGCGTGTTCC
TAAAACCGAGGCTGCGGGCCGTATCCACACGTCTACTGTGACTGTGGCGGTGATCCCAGAGGTTGAAGTTTCTGAAATCA
AGATCCCGATGTCTGACGTGAGAATTGAGACCATGCGTTCTCAAGGTTCCGGCGGTCAGTCGGTGAACAGAACTGAATCC
GCGGTTCGTGTCGTGCATTTGCCGACAGGTATCGATGTGAAATGTCAGGAAGGTAAGTCGCAGTCCACGAACCGTGAGCG
TGCTTTCCAGATCCTTTACGCAAAACTGCAGCAGATCGAGGACGAAAAGGCCCGCAAAGAGGCCTCCGATGTCCGTCTGG
AGCAGATCGGTACGGGAGATCGCTCTGAGCGCATCCGCACCTACAACTTCCCTCAGACCCGTATCACCGATCACCGCATC
GGTTTGACGATCCACCAGCTGGATCAGGTTATGAGCGGTTCATTTGAGTTGCTGATTGATCCACTCATTGCTAACTTCCA
GGCAGAGGCACTTAAAAAACAAGTCTCTGCTTAA

Upstream 100 bases:

>100_bases
ATCTTCGAACCGCATTTTCGTTTTCAGCGATGGTCCCAATCCCGATCAGCATGATGTATCAGACCTATCAATCGATCCGT
TAGTACCAGGAGCCTCAGCC

Downstream 100 bases:

>100_bases
TGAATGAAACTAAAAGAAATACTCGATAAAACCACAGCTTTCTTCAAAGACAAAAAAATCGACACTCCACGTTTGGATGC
GGAACTGCTTTTGGCCCACG

Product: peptide chain release factor RF-1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MFSKLDAVESRYEEVNMALQRPDIASNQTQYRALMKELGNLEKIVLVYRDYRKKTENLKASKELLTAEQDAEMRELIREE
VKELEAELPDLEQQLKIALIPKDPNDDKNTILEIRAGAGGDEAALFADELFRGYVHYASTQGWKVEMISFSEGNAGGAKE
IIASITGDSVFSKMKYESGVHRVQRVPKTEAAGRIHTSTVTVAVIPEVEVSEIKIPMSDVRIETMRSQGSGGQSVNRTES
AVRVVHLPTGIDVKCQEGKSQSTNRERAFQILYAKLQQIEDEKARKEASDVRLEQIGTGDRSERIRTYNFPQTRITDHRI
GLTIHQLDQVMSGSFELLIDPLIANFQAEALKKQVSA

Sequences:

>Translated_357_residues
MFSKLDAVESRYEEVNMALQRPDIASNQTQYRALMKELGNLEKIVLVYRDYRKKTENLKASKELLTAEQDAEMRELIREE
VKELEAELPDLEQQLKIALIPKDPNDDKNTILEIRAGAGGDEAALFADELFRGYVHYASTQGWKVEMISFSEGNAGGAKE
IIASITGDSVFSKMKYESGVHRVQRVPKTEAAGRIHTSTVTVAVIPEVEVSEIKIPMSDVRIETMRSQGSGGQSVNRTES
AVRVVHLPTGIDVKCQEGKSQSTNRERAFQILYAKLQQIEDEKARKEASDVRLEQIGTGDRSERIRTYNFPQTRITDHRI
GLTIHQLDQVMSGSFELLIDPLIANFQAEALKKQVSA
>Mature_357_residues
MFSKLDAVESRYEEVNMALQRPDIASNQTQYRALMKELGNLEKIVLVYRDYRKKTENLKASKELLTAEQDAEMRELIREE
VKELEAELPDLEQQLKIALIPKDPNDDKNTILEIRAGAGGDEAALFADELFRGYVHYASTQGWKVEMISFSEGNAGGAKE
IIASITGDSVFSKMKYESGVHRVQRVPKTEAAGRIHTSTVTVAVIPEVEVSEIKIPMSDVRIETMRSQGSGGQSVNRTES
AVRVVHLPTGIDVKCQEGKSQSTNRERAFQILYAKLQQIEDEKARKEASDVRLEQIGTGDRSERIRTYNFPQTRITDHRI
GLTIHQLDQVMSGSFELLIDPLIANFQAEALKKQVSA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=308, Percent_Identity=41.8831168831169, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI34577120, Length=357, Percent_Identity=34.733893557423, Blast_Score=231, Evalue=7e-61,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=41.5458937198068, Blast_Score=171, Evalue=9e-43,
Organism=Escherichia coli, GI1787462, Length=352, Percent_Identity=47.7272727272727, Blast_Score=333, Evalue=9e-93,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=36.6863905325444, Blast_Score=197, Evalue=8e-52,
Organism=Caenorhabditis elegans, GI17542784, Length=263, Percent_Identity=37.2623574144487, Blast_Score=178, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6321295, Length=307, Percent_Identity=37.785016286645, Blast_Score=215, Evalue=1e-56,
Organism=Drosophila melanogaster, GI19921226, Length=340, Percent_Identity=40.8823529411765, Blast_Score=221, Evalue=7e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_BDEBA (Q6MRK9)

Other databases:

- EMBL:   BX842646
- RefSeq:   NP_967095.1
- ProteinModelPortal:   Q6MRK9
- SMR:   Q6MRK9
- GeneID:   2733576
- GenomeReviews:   BX842601_GR
- KEGG:   bba:Bd0069
- NMPDR:   fig|264462.1.peg.65
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- BioCyc:   BBAC264462:BD0069-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40113; Mature: 40113

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSKLDAVESRYEEVNMALQRPDIASNQTQYRALMKELGNLEKIVLVYRDYRKKTENLKA
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
SKELLTAEQDAEMRELIREEVKELEAELPDLEQQLKIALIPKDPNDDKNTILEIRAGAGG
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHCCEEEEECCCCCCCCCEEEEEECCCCC
DEAALFADELFRGYVHYASTQGWKVEMISFSEGNAGGAKEIIASITGDSVFSKMKYESGV
CHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHH
HRVQRVPKTEAAGRIHTSTVTVAVIPEVEVSEIKIPMSDVRIETMRSQGSGGQSVNRTES
HHHHHCCCCHHCCEEEECEEEEEEECCCCHHHEECCHHHHHHHHHHCCCCCCCCCCCHHH
AVRVVHLPTGIDVKCQEGKSQSTNRERAFQILYAKLQQIEDEKARKEASDVRLEQIGTGD
CEEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSERIRTYNFPQTRITDHRIGLTIHQLDQVMSGSFELLIDPLIANFQAEALKKQVSA
CCCCCEECCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFSKLDAVESRYEEVNMALQRPDIASNQTQYRALMKELGNLEKIVLVYRDYRKKTENLKA
CCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
SKELLTAEQDAEMRELIREEVKELEAELPDLEQQLKIALIPKDPNDDKNTILEIRAGAGG
HHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCHHCCEEEEECCCCCCCCCEEEEEECCCCC
DEAALFADELFRGYVHYASTQGWKVEMISFSEGNAGGAKEIIASITGDSVFSKMKYESGV
CHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHCCHHHHHHHHHHHHH
HRVQRVPKTEAAGRIHTSTVTVAVIPEVEVSEIKIPMSDVRIETMRSQGSGGQSVNRTES
HHHHHCCCCHHCCEEEECEEEEEEECCCCHHHEECCHHHHHHHHHHCCCCCCCCCCCHHH
AVRVVHLPTGIDVKCQEGKSQSTNRERAFQILYAKLQQIEDEKARKEASDVRLEQIGTGD
CEEEEECCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RSERIRTYNFPQTRITDHRIGLTIHQLDQVMSGSFELLIDPLIANFQAEALKKQVSA
CCCCCEECCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA