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The map label for this gene is pckA

Identifier: 41409744

GI number: 41409744

Start: 4048595

End: 4050424

Strand: Direct

Name: pckA

Synonym: MAP3646

Alternate gene names: 41409744

Gene position: 4048595-4050424 (Clockwise)

Preceding gene: 41409743

Following gene: 41409747

Centisome position: 83.83

GC content: 67.05

Gene sequence:

>1830_bases
ATGACCTCAGCGACCATTCCCGGTTTGGACACAGCACCCACGAACCATCAGGGCCTGCTGTCATGGGTGCAGGAGGTCGC
TGAGCTGACCCAGCCCGACCGGGTGGTGTTCGCTGACGGCTCCGACGAGGAGTTCAACCGGCTGGCCGCCGAGCTGGTCG
AGGCGGGCACGCTGAAGAAGCTGAACGAGAAGAAGCATCCCAACTCCTACCTGGCGCTGTCCGACCCGTCCGACGTGGCG
CGGGTGGAGTCGCGGACCTACATCTGCACCGAAGAGGAAAGCGGCGCCGGGCCGACCAACAACTGGATGGACCCCACGGA
AATGCGGTCCATCATGACCGACCTGTACCGCGGCTGCATGCGCGGCCGCACCATGTGGGTGGTGCCGTTCTGCATGGGCC
CGCTGGGCGTCGAGGATCCGAAGCTGGGTGTGGAGATCACCGACTCCGAGTACGTCGTCATCTCGATGAAGGTGATGACC
CGGATGGGCAAGGCCGCGCTGGAGAAGATGGGCGACGACGGGTTCTTCGTCAAGGCGCTGCACTCGGTGGGGGCGCCGCT
GGAGCCGGGCCAGAAGGACGTGCCGTGGCCGTGCAACGACACCAAATACATCACCCACTTCCCGGAGACCCGCGAGATCT
GGAGTTACGGCTCGGGCTACGGCGGCAACGCCCTGCTGGGCAAGAAGTGCTACTCGCTGCGCATCGCCTCGGCGATGGCC
CACGACGAGGGCTGGCTGGCCGAGCACATGCTGATCCTCAAGCTCATCTCCCCGGAGAACAAGGCGTACTACTTCGCGGC
GGCGTTCCCGTCGGCGTGCGGCAAGACCAACCTCGCGATGCTGCAGCCCACCATCCCGGGCTGGCGCGCCGAGACGCTCG
GTGACGACATCGCCTGGATGCGGTTCGGCAAGGACGGCCGGCTGTACGCGGTCAACCCCGAGTTCGGTTTCTTCGGCGTC
GCGCCGGGCACCAACTGGAAGTCCAACCCCAACGCCATGCGGACCATCGAGGCCGGCAACACCGTCTTCACCAACGTGGC
GCTCACCGACGACAACGACGTGTGGTGGGAGGGCCTGGAGGGCCAGCCCGAGCACCTGATCGACTGGAAGGGCAACGACT
GGTACGCAGGCAAGACGGAAACGCCTGCGGCGCACCCGAATTCGCGTTACTGCACGCCGATGTCGCAGTGCCCGATCCTG
GCGCCGGAGTGGGACGACCCGCAGGGTGTGCCGATCTCGGGCATCCTGTTCGGCGCCCGCCGCAAGACCACGGTGCCGCT
GGTGACCCAGGCCCGCGACTGGCAGCACGGCGTGTTCATGGGCGCCACCATGGGCAGCGAGCAGACCGCCGCCGCCGAGG
GCAAGGTCGGCACCGTCCGCCGCGACCCGATGGCCATGCTGCCGTTCCTGGGCTACCACGTCGGCGACTACTTCCAGCAC
TGGATCGACCTGGGCAAGAACTCCGACGAGTCCAAGTTGCCGAAGGTGTTCTTCGTCAACTGGTTCCGCCGCGGCGACGA
CGGCGGCTTCCTGTGGCCCGGCTTCGGCGAGAACAGCCGGGTGCTCAAGTGGATCGTCGACCGCATCGAGCACAAGGCGG
GTGGCCAGGACACCCCGATCGGCATCGTGCCCAAGGCCGAGGACCTCGACCTGGACGGCCTGGACGTCAGCGTCGACGAC
GTCGCCAAGGCACTGGCGGTCAACCCCGACGAGTGGCGCGAGGAGCTGCCGCAGATCGAGGAGTGGTTCGAGTTCGTCGG
CGAGAAGCTGCCGACCGGCGTCCGCGACGAGTTCGAGGCGCTCAAGCAGCGGCTGTCCGAAGCCGGCTGA

Upstream 100 bases:

>100_bases
TTCCTCACGATAACCTGTAGTTAACGCCACCAAGTCCTTAATTTTGTGGGCGAAGGCATACCGAGCATCAGCCCGATACG
CAACGAAGCAGGAGAGTTCG

Downstream 100 bases:

>100_bases
CCCGGCTCAGCCCGATTCAGCCGGCGGTTCGGCGGGCCTTCTTCTCGACGTACATCAGCTCGTCAGCGCGGGCCAGCAGT
TCGTCCACGCTGGCGGTGTC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 609; Mature: 608

Protein sequence:

>609_residues
MTSATIPGLDTAPTNHQGLLSWVQEVAELTQPDRVVFADGSDEEFNRLAAELVEAGTLKKLNEKKHPNSYLALSDPSDVA
RVESRTYICTEEESGAGPTNNWMDPTEMRSIMTDLYRGCMRGRTMWVVPFCMGPLGVEDPKLGVEITDSEYVVISMKVMT
RMGKAALEKMGDDGFFVKALHSVGAPLEPGQKDVPWPCNDTKYITHFPETREIWSYGSGYGGNALLGKKCYSLRIASAMA
HDEGWLAEHMLILKLISPENKAYYFAAAFPSACGKTNLAMLQPTIPGWRAETLGDDIAWMRFGKDGRLYAVNPEFGFFGV
APGTNWKSNPNAMRTIEAGNTVFTNVALTDDNDVWWEGLEGQPEHLIDWKGNDWYAGKTETPAAHPNSRYCTPMSQCPIL
APEWDDPQGVPISGILFGARRKTTVPLVTQARDWQHGVFMGATMGSEQTAAAEGKVGTVRRDPMAMLPFLGYHVGDYFQH
WIDLGKNSDESKLPKVFFVNWFRRGDDGGFLWPGFGENSRVLKWIVDRIEHKAGGQDTPIGIVPKAEDLDLDGLDVSVDD
VAKALAVNPDEWREELPQIEEWFEFVGEKLPTGVRDEFEALKQRLSEAG

Sequences:

>Translated_609_residues
MTSATIPGLDTAPTNHQGLLSWVQEVAELTQPDRVVFADGSDEEFNRLAAELVEAGTLKKLNEKKHPNSYLALSDPSDVA
RVESRTYICTEEESGAGPTNNWMDPTEMRSIMTDLYRGCMRGRTMWVVPFCMGPLGVEDPKLGVEITDSEYVVISMKVMT
RMGKAALEKMGDDGFFVKALHSVGAPLEPGQKDVPWPCNDTKYITHFPETREIWSYGSGYGGNALLGKKCYSLRIASAMA
HDEGWLAEHMLILKLISPENKAYYFAAAFPSACGKTNLAMLQPTIPGWRAETLGDDIAWMRFGKDGRLYAVNPEFGFFGV
APGTNWKSNPNAMRTIEAGNTVFTNVALTDDNDVWWEGLEGQPEHLIDWKGNDWYAGKTETPAAHPNSRYCTPMSQCPIL
APEWDDPQGVPISGILFGARRKTTVPLVTQARDWQHGVFMGATMGSEQTAAAEGKVGTVRRDPMAMLPFLGYHVGDYFQH
WIDLGKNSDESKLPKVFFVNWFRRGDDGGFLWPGFGENSRVLKWIVDRIEHKAGGQDTPIGIVPKAEDLDLDGLDVSVDD
VAKALAVNPDEWREELPQIEEWFEFVGEKLPTGVRDEFEALKQRLSEAG
>Mature_608_residues
TSATIPGLDTAPTNHQGLLSWVQEVAELTQPDRVVFADGSDEEFNRLAAELVEAGTLKKLNEKKHPNSYLALSDPSDVAR
VESRTYICTEEESGAGPTNNWMDPTEMRSIMTDLYRGCMRGRTMWVVPFCMGPLGVEDPKLGVEITDSEYVVISMKVMTR
MGKAALEKMGDDGFFVKALHSVGAPLEPGQKDVPWPCNDTKYITHFPETREIWSYGSGYGGNALLGKKCYSLRIASAMAH
DEGWLAEHMLILKLISPENKAYYFAAAFPSACGKTNLAMLQPTIPGWRAETLGDDIAWMRFGKDGRLYAVNPEFGFFGVA
PGTNWKSNPNAMRTIEAGNTVFTNVALTDDNDVWWEGLEGQPEHLIDWKGNDWYAGKTETPAAHPNSRYCTPMSQCPILA
PEWDDPQGVPISGILFGARRKTTVPLVTQARDWQHGVFMGATMGSEQTAAAEGKVGTVRRDPMAMLPFLGYHVGDYFQHW
IDLGKNSDESKLPKVFFVNWFRRGDDGGFLWPGFGENSRVLKWIVDRIEHKAGGQDTPIGIVPKAEDLDLDGLDVSVDDV
AKALAVNPDEWREELPQIEEWFEFVGEKLPTGVRDEFEALKQRLSEAG

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family

Homologues:

Organism=Homo sapiens, GI187281517, Length=614, Percent_Identity=51.4657980456026, Blast_Score=619, Evalue=1e-177,
Organism=Homo sapiens, GI66346721, Length=606, Percent_Identity=50.4950495049505, Blast_Score=593, Evalue=1e-169,
Organism=Homo sapiens, GI66346723, Length=375, Percent_Identity=52, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71989645, Length=603, Percent_Identity=47.5953565505804, Blast_Score=568, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI71989658, Length=603, Percent_Identity=47.5953565505804, Blast_Score=566, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI71989653, Length=594, Percent_Identity=47.6430976430976, Blast_Score=559, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI17555492, Length=615, Percent_Identity=48.9430894308943, Blast_Score=557, Evalue=1e-159,
Organism=Caenorhabditis elegans, GI32564411, Length=540, Percent_Identity=51.4814814814815, Blast_Score=531, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI32564407, Length=527, Percent_Identity=50.853889943074, Blast_Score=508, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI71989649, Length=482, Percent_Identity=49.5850622406639, Blast_Score=484, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI32564409, Length=373, Percent_Identity=49.597855227882, Blast_Score=345, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI17540956, Length=502, Percent_Identity=24.3027888446215, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24655085, Length=603, Percent_Identity=49.9170812603648, Blast_Score=590, Evalue=1e-168,
Organism=Drosophila melanogaster, GI24655082, Length=601, Percent_Identity=50.5823627287854, Blast_Score=587, Evalue=1e-167,
Organism=Drosophila melanogaster, GI161077226, Length=571, Percent_Identity=51.3134851138354, Blast_Score=564, Evalue=1e-161,
Organism=Drosophila melanogaster, GI24655088, Length=504, Percent_Identity=51.3888888888889, Blast_Score=518, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PCKG_MYCPA (Q73TS2)

Other databases:

- EMBL:   AE016958
- RefSeq:   NP_962580.1
- ProteinModelPortal:   Q73TS2
- SMR:   Q73TS2
- EnsemblBacteria:   EBMYCT00000040436
- GeneID:   2719651
- GenomeReviews:   AE016958_GR
- KEGG:   mpa:MAP3646
- NMPDR:   fig|262316.1.peg.3646
- GeneTree:   EBGT00050000015941
- HOGENOM:   HBG484437
- OMA:   CGYHMGD
- ProtClustDB:   PRK04210
- BRENDA:   4.1.1.32
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00452
- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PANTHER:   PTHR11561
- PIRSF:   PIRSF001348

Pfam domain/function: PF00821 PEPCK; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.32

Molecular weight: Translated: 67660; Mature: 67529

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00505 PEPCK_GTP

Important sites: ACT_SITE 273-273 BINDING 81-81 BINDING 222-222 BINDING 229-229 BINDING 271-271 BINDING 389-389 BINDING 420-420

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSATIPGLDTAPTNHQGLLSWVQEVAELTQPDRVVFADGSDEEFNRLAAELVEAGTLKK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
LNEKKHPNSYLALSDPSDVARVESRTYICTEEESGAGPTNNWMDPTEMRSIMTDLYRGCM
HHHCCCCCCEEEECCCHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RGRTMWVVPFCMGPLGVEDPKLGVEITDSEYVVISMKVMTRMGKAALEKMGDDGFFVKAL
CCCEEEEEEECCCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHCCCCCHHHHHH
HSVGAPLEPGQKDVPWPCNDTKYITHFPETREIWSYGSGYGGNALLGKKCYSLRIASAMA
HHCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
HDEGWLAEHMLILKLISPENKAYYFAAAFPSACGKTNLAMLQPTIPGWRAETLGDDIAWM
CCCCHHHHHHHHHHEECCCCCEEEEEEECCCCCCCCCEEEECCCCCCCCHHHCCCCEEEE
RFGKDGRLYAVNPEFGFFGVAPGTNWKSNPNAMRTIEAGNTVFTNVALTDDNDVWWEGLE
EECCCCCEEEECCCCCEEECCCCCCCCCCCCCEEEEECCCEEEEEEEEECCCCCEECCCC
GQPEHLIDWKGNDWYAGKTETPAAHPNSRYCTPMSQCPILAPEWDDPQGVPISGILFGAR
CCCCCEEEECCCCEECCCCCCCCCCCCCCCCCCHHHCCEECCCCCCCCCCCEEEEEECCC
RKTTVPLVTQARDWQHGVFMGATMGSEQTAAAEGKVGTVRRDPMAMLPFLGYHVGDYFQH
CCCCCCEEECCCCCCCCEEEEECCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
WIDLGKNSDESKLPKVFFVNWFRRGDDGGFLWPGFGENSRVLKWIVDRIEHKAGGQDTPI
HHHCCCCCCHHHCCEEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCCE
GIVPKAEDLDLDGLDVSVDDVAKALAVNPDEWREELPQIEEWFEFVGEKLPTGVRDEFEA
EECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LKQRLSEAG
HHHHHHHCC
>Mature Secondary Structure 
TSATIPGLDTAPTNHQGLLSWVQEVAELTQPDRVVFADGSDEEFNRLAAELVEAGTLKK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHH
LNEKKHPNSYLALSDPSDVARVESRTYICTEEESGAGPTNNWMDPTEMRSIMTDLYRGCM
HHHCCCCCCEEEECCCHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RGRTMWVVPFCMGPLGVEDPKLGVEITDSEYVVISMKVMTRMGKAALEKMGDDGFFVKAL
CCCEEEEEEECCCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHCCCCCHHHHHH
HSVGAPLEPGQKDVPWPCNDTKYITHFPETREIWSYGSGYGGNALLGKKCYSLRIASAMA
HHCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
HDEGWLAEHMLILKLISPENKAYYFAAAFPSACGKTNLAMLQPTIPGWRAETLGDDIAWM
CCCCHHHHHHHHHHEECCCCCEEEEEEECCCCCCCCCEEEECCCCCCCCHHHCCCCEEEE
RFGKDGRLYAVNPEFGFFGVAPGTNWKSNPNAMRTIEAGNTVFTNVALTDDNDVWWEGLE
EECCCCCEEEECCCCCEEECCCCCCCCCCCCCEEEEECCCEEEEEEEEECCCCCEECCCC
GQPEHLIDWKGNDWYAGKTETPAAHPNSRYCTPMSQCPILAPEWDDPQGVPISGILFGAR
CCCCCEEEECCCCEECCCCCCCCCCCCCCCCCCHHHCCEECCCCCCCCCCCEEEEEECCC
RKTTVPLVTQARDWQHGVFMGATMGSEQTAAAEGKVGTVRRDPMAMLPFLGYHVGDYFQH
CCCCCCEEECCCCCCCCEEEEECCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
WIDLGKNSDESKLPKVFFVNWFRRGDDGGFLWPGFGENSRVLKWIVDRIEHKAGGQDTPI
HHHCCCCCCHHHCCEEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCCE
GIVPKAEDLDLDGLDVSVDDVAKALAVNPDEWREELPQIEEWFEFVGEKLPTGVRDEFEA
EECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LKQRLSEAG
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA