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The map label for this gene is fadD4

Identifier: 41409747

GI number: 41409747

Start: 4051673

End: 4053190

Strand: Direct

Name: fadD4

Synonym: MAP3649

Alternate gene names: 41409747

Gene position: 4051673-4053190 (Clockwise)

Preceding gene: 41409744

Following gene: 41409748

Centisome position: 83.89

GC content: 66.4

Gene sequence:

>1518_bases
ATGCAGATCCGCGAGTATCTCGGCGCCGGCAAGCCCGCCGTCATCCTCTACCCGTCCGGCACGGTCGTCACGTTCGACGA
CCTGGAAGCGCGCGCGAATCGGCTGGCCCACCGGTTCCGCAAGGCGGGTCTGCGCGAGGGCGACACGGTCGCCATCCTGA
TGGAGAACAACGAGCACATTCACGCGGTGATGTGGGCGGCCCGCCGCAGCGGCCTGTACTACGTGCCGATCAACACCCAT
CTGACCGCCGCCGAGGCCGCCTACATCGTCGACAACAGCTCGGCCAGGGCGATCGTCGGTTCGGCGGCGTTGCGCGACAC
CTGCGCGCGGATCGGCGAGCACCTGCCCGGCGGTCTGCCGGATCTGCTGCTGATGGCCGACGGCGACCTCGACGGCTGGG
AGCACTACCCGGAATGCGTTGCCGGCGAACCCGATACGCCCATCGACGACGAGCTCGAGGGCGACCTGCTGCAGTACTCG
TCGGGAACCACGGGACGGCCCAAGGGCATCAAACGCGAACTGCCGCATGTCCATCCGGCCGAAGCCCCAGGCATGATGTC
GGCGCTGGTCGGCTTCTGGATGACGCCCGAGTCGATCTACCTCAGCCCGGCTCCGCTGTACCACACCGCGCCGTCGGTGT
GGTCGATGAGCGCGCAGGCCGGCGGCATCACCACGGTGGTGATGGAGAAATTCGACGCCGAGGGCTGCCTGGACGCCATC
CAGCGGCACCGGGTCACGCACGGCCAGTTCGTCCCGGCGATGTTCACCCGCATGCTGAAACTGCCTGAAGCCGTTCGTCA
TTCGCACGACCTGAGCAGCCTGCAGCGGGTGATGCACGCGGCGGCGCCCTGCCCGGTGGAGATCAAGAAGCAGATGATCG
ACTGGTGGGGCCCGATCATCGACGAGTATTACGCCTCCTCGGAGGCCATCGGCTCGACGCTGATCAGCGCGGAGGAGTGG
CTGGCCCATCCCGGTTCGGTGGGCAAACCGATGGCCTGCGAGATCCACATCCTCGACGAGAACGGCAACGAGCTGCCACC
GGGGCAGGCGGGCGAGATCTATTTCAGCGGAGGGTATTCCTTCGAATACCTCAACGATGAGGCCAAGACCGCCGCTTCCC
GCGACAAGCACGGCTGGGTCACCGTCGGCGACGTCGGCTACGTCGACGAGGAGGGCTACCTGTACCTGACCGACCGGCGC
CACCACATGATCATCTCCGGCGGGGTGAACATCTATCCGCAAGAAGCGGAGAACCTTCTGGTCACCCACCCCAAGGTGCT
GGACGCCGCGGTGTTCGGGGTGCCCGACGACGAGATGGGCCAGCGGGTGATGGCGGCCGTGCAGACCGTCGATCCCGGCG
ACGCCACCGACGAATTCGGCGCCGAGCTGCTGTCCTGGCTGCGGGATCGGTTGGCGCACTACAAGTGTCCGCGAGCGATC
GCATTCGAGGAGCAGCTGCCGCGCACCGACACTGGCAAGCTCTTCAAGAACGGGCTGATCGAGAAGTATTCGGTGTGA

Upstream 100 bases:

>100_bases
GACGTCGACGACAGTGTAAGTATCGGCGCCGTCTTCGGCTCGCCGACCGGCTTGCCGACCACTTGACACCCGTCAAGTTT
GCCGGCGGTACAGTCTGCGC

Downstream 100 bases:

>100_bases
CCGATGCTGCGGGTGGTCGACCTCTCCAGCGCGCCGCAGGACGGCCCCGCCTCACCGCCGGGTGTGATCGTCGCCGTCGG
ATCGGCTGACCAGATTGCCC

Product: long-chain-fatty-acid--CoA ligase

Products: AMP; diphosphate; acyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MQIREYLGAGKPAVILYPSGTVVTFDDLEARANRLAHRFRKAGLREGDTVAILMENNEHIHAVMWAARRSGLYYVPINTH
LTAAEAAYIVDNSSARAIVGSAALRDTCARIGEHLPGGLPDLLLMADGDLDGWEHYPECVAGEPDTPIDDELEGDLLQYS
SGTTGRPKGIKRELPHVHPAEAPGMMSALVGFWMTPESIYLSPAPLYHTAPSVWSMSAQAGGITTVVMEKFDAEGCLDAI
QRHRVTHGQFVPAMFTRMLKLPEAVRHSHDLSSLQRVMHAAAPCPVEIKKQMIDWWGPIIDEYYASSEAIGSTLISAEEW
LAHPGSVGKPMACEIHILDENGNELPPGQAGEIYFSGGYSFEYLNDEAKTAASRDKHGWVTVGDVGYVDEEGYLYLTDRR
HHMIISGGVNIYPQEAENLLVTHPKVLDAAVFGVPDDEMGQRVMAAVQTVDPGDATDEFGAELLSWLRDRLAHYKCPRAI
AFEEQLPRTDTGKLFKNGLIEKYSV

Sequences:

>Translated_505_residues
MQIREYLGAGKPAVILYPSGTVVTFDDLEARANRLAHRFRKAGLREGDTVAILMENNEHIHAVMWAARRSGLYYVPINTH
LTAAEAAYIVDNSSARAIVGSAALRDTCARIGEHLPGGLPDLLLMADGDLDGWEHYPECVAGEPDTPIDDELEGDLLQYS
SGTTGRPKGIKRELPHVHPAEAPGMMSALVGFWMTPESIYLSPAPLYHTAPSVWSMSAQAGGITTVVMEKFDAEGCLDAI
QRHRVTHGQFVPAMFTRMLKLPEAVRHSHDLSSLQRVMHAAAPCPVEIKKQMIDWWGPIIDEYYASSEAIGSTLISAEEW
LAHPGSVGKPMACEIHILDENGNELPPGQAGEIYFSGGYSFEYLNDEAKTAASRDKHGWVTVGDVGYVDEEGYLYLTDRR
HHMIISGGVNIYPQEAENLLVTHPKVLDAAVFGVPDDEMGQRVMAAVQTVDPGDATDEFGAELLSWLRDRLAHYKCPRAI
AFEEQLPRTDTGKLFKNGLIEKYSV
>Mature_505_residues
MQIREYLGAGKPAVILYPSGTVVTFDDLEARANRLAHRFRKAGLREGDTVAILMENNEHIHAVMWAARRSGLYYVPINTH
LTAAEAAYIVDNSSARAIVGSAALRDTCARIGEHLPGGLPDLLLMADGDLDGWEHYPECVAGEPDTPIDDELEGDLLQYS
SGTTGRPKGIKRELPHVHPAEAPGMMSALVGFWMTPESIYLSPAPLYHTAPSVWSMSAQAGGITTVVMEKFDAEGCLDAI
QRHRVTHGQFVPAMFTRMLKLPEAVRHSHDLSSLQRVMHAAAPCPVEIKKQMIDWWGPIIDEYYASSEAIGSTLISAEEW
LAHPGSVGKPMACEIHILDENGNELPPGQAGEIYFSGGYSFEYLNDEAKTAASRDKHGWVTVGDVGYVDEEGYLYLTDRR
HHMIISGGVNIYPQEAENLLVTHPKVLDAAVFGVPDDEMGQRVMAAVQTVDPGDATDEFGAELLSWLRDRLAHYKCPRAI
AFEEQLPRTDTGKLFKNGLIEKYSV

Specific function: Active with a large number of C-24 bile acids possessing unhindered C-24 carboxyl groups. Requires ATP and produces AMP and pyrophosphate in addition to the bile-acid CoA thioester [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=529, Percent_Identity=27.4102079395085, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI122937307, Length=506, Percent_Identity=27.8656126482213, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI42544132, Length=509, Percent_Identity=26.9155206286837, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI157311624, Length=511, Percent_Identity=26.027397260274, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI157311622, Length=511, Percent_Identity=26.027397260274, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI187761345, Length=528, Percent_Identity=26.5151515151515, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI187761343, Length=528, Percent_Identity=26.5151515151515, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI58082049, Length=506, Percent_Identity=26.0869565217391, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI38505220, Length=502, Percent_Identity=26.2948207171315, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI115511026, Length=503, Percent_Identity=26.2425447316103, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI62865631, Length=528, Percent_Identity=24.6212121212121, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI13325055, Length=528, Percent_Identity=24.6212121212121, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI13375727, Length=559, Percent_Identity=25.4025044722719, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI40807357, Length=537, Percent_Identity=25.8845437616387, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI38524616, Length=525, Percent_Identity=25.3333333333333, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI8923896, Length=545, Percent_Identity=23.302752293578, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI253970489, Length=558, Percent_Identity=22.7598566308244, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI221142682, Length=518, Percent_Identity=29.1505791505791, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1788107, Length=523, Percent_Identity=26.5774378585086, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI145693145, Length=496, Percent_Identity=25.2016129032258, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1786810, Length=499, Percent_Identity=24.8496993987976, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI1790505, Length=525, Percent_Identity=22.8571428571429, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1786529, Length=528, Percent_Identity=22.1590909090909, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788595, Length=322, Percent_Identity=26.7080745341615, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71994703, Length=533, Percent_Identity=26.8292682926829, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI71994690, Length=533, Percent_Identity=26.8292682926829, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI71994694, Length=533, Percent_Identity=26.8292682926829, Blast_Score=159, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI32563687, Length=502, Percent_Identity=25.0996015936255, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17531443, Length=495, Percent_Identity=25.2525252525253, Blast_Score=145, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17558820, Length=506, Percent_Identity=26.6798418972332, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17538037, Length=529, Percent_Identity=27.0321361058601, Blast_Score=132, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17560308, Length=516, Percent_Identity=26.937984496124, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17559526, Length=379, Percent_Identity=28.4960422163588, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17557194, Length=533, Percent_Identity=26.266416510319, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17551278, Length=545, Percent_Identity=26.0550458715596, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI17560140, Length=502, Percent_Identity=24.5019920318725, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI115534440, Length=547, Percent_Identity=24.6800731261426, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71996755, Length=493, Percent_Identity=22.920892494929, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6319699, Length=363, Percent_Identity=27.8236914600551, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6319264, Length=534, Percent_Identity=23.0337078651685, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21355181, Length=489, Percent_Identity=27.19836400818, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24581924, Length=538, Percent_Identity=27.6951672862454, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI18859661, Length=496, Percent_Identity=25.2016129032258, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24656500, Length=517, Percent_Identity=23.9845261121857, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI19922652, Length=521, Percent_Identity=24.1842610364683, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21358303, Length=455, Percent_Identity=23.0769230769231, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24648257, Length=511, Percent_Identity=23.679060665362, Blast_Score=112, Evalue=6e-25,
Organism=Drosophila melanogaster, GI24648260, Length=499, Percent_Identity=24.248496993988, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24648253, Length=453, Percent_Identity=24.7240618101545, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24648255, Length=453, Percent_Identity=24.7240618101545, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45550496, Length=519, Percent_Identity=25.2408477842004, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45552801, Length=521, Percent_Identity=25.9117082533589, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI21356441, Length=446, Percent_Identity=22.6457399103139, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI281365686, Length=505, Percent_Identity=24.1584158415842, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21356947, Length=493, Percent_Identity=23.7322515212982, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24653035, Length=503, Percent_Identity=23.2604373757455, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI20130357, Length=489, Percent_Identity=23.5173824130879, Blast_Score=99, Evalue=9e-21,
Organism=Drosophila melanogaster, GI21355225, Length=410, Percent_Identity=25.3658536585366, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI161076582, Length=513, Percent_Identity=22.2222222222222, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17933690, Length=568, Percent_Identity=23.943661971831, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI62472339, Length=538, Percent_Identity=21.9330855018587, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24667955, Length=538, Percent_Identity=21.9330855018587, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24667959, Length=364, Percent_Identity=23.6263736263736, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3

Molecular weight: Translated: 55518; Mature: 55518

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIREYLGAGKPAVILYPSGTVVTFDDLEARANRLAHRFRKAGLREGDTVAILMENNEHI
CCCHHHHCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCE
HAVMWAARRSGLYYVPINTHLTAAEAAYIVDNSSARAIVGSAALRDTCARIGEHLPGGLP
EEEEEHHHHCCEEEEECCCCEECCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCCC
DLLLMADGDLDGWEHYPECVAGEPDTPIDDELEGDLLQYSSGTTGRPKGIKRELPHVHPA
CEEEEECCCCCCHHHCCHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHCCCCCCC
EAPGMMSALVGFWMTPESIYLSPAPLYHTAPSVWSMSAQAGGITTVVMEKFDAEGCLDAI
CCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHCCCCCCCCEEEEHHHHHCHHHHHHHH
QRHRVTHGQFVPAMFTRMLKLPEAVRHSHDLSSLQRVMHAAAPCPVEIKKQMIDWWGPII
HHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
DEYYASSEAIGSTLISAEEWLAHPGSVGKPMACEIHILDENGNELPPGQAGEIYFSGGYS
HHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCEE
FEYLNDEAKTAASRDKHGWVTVGDVGYVDEEGYLYLTDRRHHMIISGGVNIYPQEAENLL
EEEECCHHHHHHCCCCCCCEEECCCCEECCCCEEEEECCCEEEEEECCCEECCCCCCCEE
VTHPKVLDAAVFGVPDDEMGQRVMAAVQTVDPGDATDEFGAELLSWLRDRLAHYKCPRAI
EECCHHHHHHEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
AFEEQLPRTDTGKLFKNGLIEKYSV
EHHHHCCCCCCHHHHHCCCCHHCCC
>Mature Secondary Structure
MQIREYLGAGKPAVILYPSGTVVTFDDLEARANRLAHRFRKAGLREGDTVAILMENNEHI
CCCHHHHCCCCCEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCE
HAVMWAARRSGLYYVPINTHLTAAEAAYIVDNSSARAIVGSAALRDTCARIGEHLPGGLP
EEEEEHHHHCCEEEEECCCCEECCCEEEEEECCCCCEEHHHHHHHHHHHHHHHHCCCCCC
DLLLMADGDLDGWEHYPECVAGEPDTPIDDELEGDLLQYSSGTTGRPKGIKRELPHVHPA
CEEEEECCCCCCHHHCCHHHCCCCCCCCCCCCCCCCEEECCCCCCCCCCHHHHCCCCCCC
EAPGMMSALVGFWMTPESIYLSPAPLYHTAPSVWSMSAQAGGITTVVMEKFDAEGCLDAI
CCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHCCCCCCCCEEEEHHHHHCHHHHHHHH
QRHRVTHGQFVPAMFTRMLKLPEAVRHSHDLSSLQRVMHAAAPCPVEIKKQMIDWWGPII
HHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
DEYYASSEAIGSTLISAEEWLAHPGSVGKPMACEIHILDENGNELPPGQAGEIYFSGGYS
HHHHCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEECCEE
FEYLNDEAKTAASRDKHGWVTVGDVGYVDEEGYLYLTDRRHHMIISGGVNIYPQEAENLL
EEEECCHHHHHHCCCCCCCEEECCCCEECCCCEEEEECCCEEEEEECCCEECCCCCCCEE
VTHPKVLDAAVFGVPDDEMGQRVMAAVQTVDPGDATDEFGAELLSWLRDRLAHYKCPRAI
EECCHHHHHHEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
AFEEQLPRTDTGKLFKNGLIEKYSV
EHHHHCCCCCCHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; long-chain carboxylic acid; CoA

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate + an acyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2254270; 1551828 [H]