| Definition | Mycobacterium avium subsp. paratuberculosis K-10, complete genome. |
|---|---|
| Accession | NC_002944 |
| Length | 4,829,781 |
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The map label for this gene is cheR [C]
Identifier: 41409331
GI number: 41409331
Start: 3587161
End: 3589011
Strand: Reverse
Name: cheR [C]
Synonym: MAP3233c
Alternate gene names: 41409331
Gene position: 3589011-3587161 (Counterclockwise)
Preceding gene: 41409332
Following gene: 41409329
Centisome position: 74.31
GC content: 67.1
Gene sequence:
>1851_bases ATGAATTCCACGACTGACGAGGCCGACGAGGCATTCGAGGCCTTGTTGCGGTACATGCGGGATTCGCGCGGCTTCGACTT CACCGGCTACAAGCGCACCTCGCTGATGCGCCGCGTCCGGCACCGGATGGACCAGGCGGGCTACACCGCCTTCGACGAGT ACCTGGACTTCCTGCAGGCCAGCTCCGACGAGTTCACCGCGCTGTTCAACACCATCCTGATCAACGTCACCTCGTTCTTC CGCGACCCGGACGCCTGGGAGTTCGTCGCCGCCGAAGTCATTCCGCGACTGCTGGCCGAACGCGGGCCCAACGATCCGAT CCGGGTGTGGAGTGCGGGGTGCGCCTCGGGCCAGGAGGCCTACACGCTGGCGATGCTGCTGGCCGAGGCGCTGGGCTTCG ACGCGTTCCGGCAGCGGGTGAAGATCTATGCCACCGACGTCGACGAGGACGCGCTGACCGAGGCGCGCGGCGCCTCCTAC GACGCCCGGGCGGTCGAGTCGGTGCCGGCGGATCTGCTGGCCCGCTACTTCGAACAGGTCAACGGCCGCTACGTTTTTCA CAAGGATCTGCGCCGGGCGGTGATCTTCGGCCGCAACGACCTGGTCAAGGATGCGCCGATCTCCCGGGTGGACCTGCTGG TGTGCCGCAACACGCTGATGTATCTGAACGCCGAGACCCAGCGAAATGTGTTGGGGCGCTTGCATTTTGCGCTTGCCCCG CAGGGCACGCTGTTTCTGGGGCACGCCGAGATGCTGCTCAGCCACGGTGACCGGTTCACCCCGCTGAGCCTCAAGCACCG GATCTTCCGCAAGGCGGTCGGCAGCCACAGCGGCCTGGACCACTACGACCCGGCGGGCGCGTTCTACGAACGCCACGCCG ACCTGCCGGGGCTGGCAACGGTGCGCGATCTGGCGTTCCGGGCCAGCCCGGTGGCCCAGATCGTGGTGACCGGCGAGGAC ACCGTCGCGATGATCAACCAGCAGGCCGAGACGGTCTTCGGGCTCTCGGCGCGCGACATCGGCCGGCTGCTGCGCGATCT CGAGGTGTCCTACCGTCCCGTCGAGCTGCGCGCCTACCTGGAGCAGGCCAAGGTGGAGCGGCGCTCGGCGCGCATCCAGG ACGTCAAATGGCAGCGGCCCGGCGCCGAGACGGTGTGGTTCGAGATCCACGTCAACCCGCTCGTCGACGCCGAGAACGGC CTGCTCGGCGTGTCCATCGTGTTCTTCGACGTGACCGCGACGCGCGCGCTGCTGGACAAGGTGGTGCAGACCAACCGCCA GCTGGAGGCGGCCTACGAGGAGCTGCAGTCCACCAACGAGGAACTCGAAACCACCAACGAGGAACTGCAGTCCACGGTCG AGGAGCTCGAGACCACCAACGAGGAGCTGCAGTCCACCAACGAAGAACTCGAGACGATGAACGAGGAGCTGCAGTCCACC AACGACGAACTGCACACCATCAACGACATGCTGCGCGAACGCAGCCTGGAACTCGACGACGCGAAGAGCTTCCTGGATTC GCTGGTCGACTCGGTGCGGATGGGCATGGTGGTGGTGGACCGCGAGATGAAGGTGATCCTGTGGAACCGCGGCTGCGAAG AGCTGTGGGGGCTGCGGGCCGACGAGACGACGGGCACCATGCTGACCGCGCTGGACATCGGGCTGCCGCTGGACGCGGTG CGTCCGCTGATCGGCAACGCGTTCGTCGACCCGGACGGCTCCGGTGAGGTCGTGGTGGATGCGGTCAACCGGCGCGGCCG CCCGGCCCGGGTGCGGGTCACCTGCACGTCGTTTCGCGGCAACGAGGGAACCGTCGGCGGGGCGCTGCTGCTGATGGACG TGGTGGGTTAG
Upstream 100 bases:
>100_bases TCCGGCAAGACCGTGTGGGGATCGGTCGGCAGGGAAGACCGGATCTGAGTCCCGCGAGTAGTGTTCAAGTCCTTCGGCAC CCTTATCAGACGGGACTTGG
Downstream 100 bases:
>100_bases CCGTCCCGGTCGCGGTGCTCCTGTTCGGAGCGCGCCCAGGCCTCGTCGGCGGCCCGGTGGGCCAGCAGGGCGCGCCGGCG CGACTCCTCGGCCCGGCGCC
Product: hypothetical protein
Products: S-adenosyl-L-homocysteine; protein L-glutamate methyl ester; Tsrglu-Me; Trgglu-Me; Targlu-Me; Tapglu-Me + [C]
Alternate protein names: ORF19 [H]
Number of amino acids: Translated: 616; Mature: 616
Protein sequence:
>616_residues MNSTTDEADEAFEALLRYMRDSRGFDFTGYKRTSLMRRVRHRMDQAGYTAFDEYLDFLQASSDEFTALFNTILINVTSFF RDPDAWEFVAAEVIPRLLAERGPNDPIRVWSAGCASGQEAYTLAMLLAEALGFDAFRQRVKIYATDVDEDALTEARGASY DARAVESVPADLLARYFEQVNGRYVFHKDLRRAVIFGRNDLVKDAPISRVDLLVCRNTLMYLNAETQRNVLGRLHFALAP QGTLFLGHAEMLLSHGDRFTPLSLKHRIFRKAVGSHSGLDHYDPAGAFYERHADLPGLATVRDLAFRASPVAQIVVTGED TVAMINQQAETVFGLSARDIGRLLRDLEVSYRPVELRAYLEQAKVERRSARIQDVKWQRPGAETVWFEIHVNPLVDAENG LLGVSIVFFDVTATRALLDKVVQTNRQLEAAYEELQSTNEELETTNEELQSTVEELETTNEELQSTNEELETMNEELQST NDELHTINDMLRERSLELDDAKSFLDSLVDSVRMGMVVVDREMKVILWNRGCEELWGLRADETTGTMLTALDIGLPLDAV RPLIGNAFVDPDGSGEVVVDAVNRRGRPARVRVTCTSFRGNEGTVGGALLLMDVVG
Sequences:
>Translated_616_residues MNSTTDEADEAFEALLRYMRDSRGFDFTGYKRTSLMRRVRHRMDQAGYTAFDEYLDFLQASSDEFTALFNTILINVTSFF RDPDAWEFVAAEVIPRLLAERGPNDPIRVWSAGCASGQEAYTLAMLLAEALGFDAFRQRVKIYATDVDEDALTEARGASY DARAVESVPADLLARYFEQVNGRYVFHKDLRRAVIFGRNDLVKDAPISRVDLLVCRNTLMYLNAETQRNVLGRLHFALAP QGTLFLGHAEMLLSHGDRFTPLSLKHRIFRKAVGSHSGLDHYDPAGAFYERHADLPGLATVRDLAFRASPVAQIVVTGED TVAMINQQAETVFGLSARDIGRLLRDLEVSYRPVELRAYLEQAKVERRSARIQDVKWQRPGAETVWFEIHVNPLVDAENG LLGVSIVFFDVTATRALLDKVVQTNRQLEAAYEELQSTNEELETTNEELQSTVEELETTNEELQSTNEELETMNEELQST NDELHTINDMLRERSLELDDAKSFLDSLVDSVRMGMVVVDREMKVILWNRGCEELWGLRADETTGTMLTALDIGLPLDAV RPLIGNAFVDPDGSGEVVVDAVNRRGRPARVRVTCTSFRGNEGTVGGALLLMDVVG >Mature_616_residues MNSTTDEADEAFEALLRYMRDSRGFDFTGYKRTSLMRRVRHRMDQAGYTAFDEYLDFLQASSDEFTALFNTILINVTSFF RDPDAWEFVAAEVIPRLLAERGPNDPIRVWSAGCASGQEAYTLAMLLAEALGFDAFRQRVKIYATDVDEDALTEARGASY DARAVESVPADLLARYFEQVNGRYVFHKDLRRAVIFGRNDLVKDAPISRVDLLVCRNTLMYLNAETQRNVLGRLHFALAP QGTLFLGHAEMLLSHGDRFTPLSLKHRIFRKAVGSHSGLDHYDPAGAFYERHADLPGLATVRDLAFRASPVAQIVVTGED TVAMINQQAETVFGLSARDIGRLLRDLEVSYRPVELRAYLEQAKVERRSARIQDVKWQRPGAETVWFEIHVNPLVDAENG LLGVSIVFFDVTATRALLDKVVQTNRQLEAAYEELQSTNEELETTNEELQSTVEELETTNEELQSTNEELETMNEELQST NDELHTINDMLRERSLELDDAKSFLDSLVDSVRMGMVVVDREMKVILWNRGCEELWGLRADETTGTMLTALDIGLPLDAV RPLIGNAFVDPDGSGEVVVDAVNRRGRPARVRVTCTSFRGNEGTVGGALLLMDVVG
Specific function: Methylation Of The Membrane-Bound Methyl-Accepting Chemotaxis Proteins (Mcp) To Form Gamma-Glutamyl Methyl Ester Residues In Mcp. [C]
COG id: COG1352
COG function: function code NT; Methylase of chemotaxis methyl-accepting proteins
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 cheR-type methyltransferase domain [H]
Homologues:
Organism=Escherichia coli, GI1788193, Length=261, Percent_Identity=29.8850574712644, Blast_Score=110, Evalue=3e-25,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR022642 - InterPro: IPR000780 - InterPro: IPR022641 - InterPro: IPR000014 - InterPro: IPR013656 - InterPro: IPR000673 [H]
Pfam domain/function: PF01339 CheB_methylest; PF01739 CheR; PF03705 CheR_N; PF08448 PAS_4 [H]
EC number: 2.1.1.80 [C]
Molecular weight: Translated: 69182; Mature: 69182
Theoretical pI: Translated: 4.54; Mature: 4.54
Prosite motif: PS50112 PAS ; PS50123 CHER
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.6 %Cys (Translated Protein) 2.4 %Met (Translated Protein) 3.1 %Cys+Met (Translated Protein) 0.6 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 3.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNSTTDEADEAFEALLRYMRDSRGFDFTGYKRTSLMRRVRHRMDQAGYTAFDEYLDFLQA CCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC SSDEFTALFNTILINVTSFFRDPDAWEFVAAEVIPRLLAERGPNDPIRVWSAGCASGQEA CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHH YTLAMLLAEALGFDAFRQRVKIYATDVDEDALTEARGASYDARAVESVPADLLARYFEQV HHHHHHHHHHHCHHHHHHCEEEEEECCCHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHH NGRYVFHKDLRRAVIFGRNDLVKDAPISRVDLLVCRNTLMYLNAETQRNVLGRLHFALAP CCCEEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCHHEEEECHHHHHHHHHHHHEECC QGTLFLGHAEMLLSHGDRFTPLSLKHRIFRKAVGSHSGLDHYDPAGAFYERHADLPGLAT CCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCHHH VRDLAFRASPVAQIVVTGEDTVAMINQQAETVFGLSARDIGRLLRDLEVSYRPVELRAYL HHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHH EQAKVERRSARIQDVKWQRPGAETVWFEIHVNPLVDAENGLLGVSIVFFDVTATRALLDK HHHHHHHHHHHHHCCEECCCCCCEEEEEEEECEEEECCCCEEEEEEEEEEHHHHHHHHHH VVQTNRQLEAAYEELQSTNEELETTNEELQSTVEELETTNEELQSTNEELETMNEELQST HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC NDELHTINDMLRERSLELDDAKSFLDSLVDSVRMGMVVVDREMKVILWNRGCEELWGLRA CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCEEEECCCCEEEEECCCHHHHCCCCC DETTGTMLTALDIGLPLDAVRPLIGNAFVDPDGSGEVVVDAVNRRGRPARVRVTCTSFRG CCCCCCEEEEHCCCCCHHHHHHHHCCCEECCCCCCCEEEEECCCCCCCEEEEEEEEECCC NEGTVGGALLLMDVVG CCCCCCHHHHHHHHCC >Mature Secondary Structure MNSTTDEADEAFEALLRYMRDSRGFDFTGYKRTSLMRRVRHRMDQAGYTAFDEYLDFLQA CCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC SSDEFTALFNTILINVTSFFRDPDAWEFVAAEVIPRLLAERGPNDPIRVWSAGCASGQEA CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHH YTLAMLLAEALGFDAFRQRVKIYATDVDEDALTEARGASYDARAVESVPADLLARYFEQV HHHHHHHHHHHCHHHHHHCEEEEEECCCHHHHHHHCCCCCCHHHHHHCCHHHHHHHHHHH NGRYVFHKDLRRAVIFGRNDLVKDAPISRVDLLVCRNTLMYLNAETQRNVLGRLHFALAP CCCEEEHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCHHEEEECHHHHHHHHHHHHEECC QGTLFLGHAEMLLSHGDRFTPLSLKHRIFRKAVGSHSGLDHYDPAGAFYERHADLPGLAT CCCEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCHHH VRDLAFRASPVAQIVVTGEDTVAMINQQAETVFGLSARDIGRLLRDLEVSYRPVELRAYL HHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCHHHHHHH EQAKVERRSARIQDVKWQRPGAETVWFEIHVNPLVDAENGLLGVSIVFFDVTATRALLDK HHHHHHHHHHHHHCCEECCCCCCEEEEEEEECEEEECCCCEEEEEEEEEEHHHHHHHHHH VVQTNRQLEAAYEELQSTNEELETTNEELQSTVEELETTNEELQSTNEELETMNEELQST HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC NDELHTINDMLRERSLELDDAKSFLDSLVDSVRMGMVVVDREMKVILWNRGCEELWGLRA CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCEEEECCCCEEEEECCCHHHHCCCCC DETTGTMLTALDIGLPLDAVRPLIGNAFVDPDGSGEVVVDAVNRRGRPARVRVTCTSFRG CCCCCCEEEEHCCCCCHHHHHHHHCCCEECCCCCCCEEEEECCCCCCCEEEEEEEEECCC NEGTVGGALLLMDVVG CCCCCCHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: Ca2+; Mg2+ [C]
Kcat value (1/min): 10 [C]
Specific activity: NA
Km value (mM): 0.017 {S-adenosyl-L-methionine}} 0.01 {S-adenosyl-L-methionine}} [C]
Substrates: S-Adenosyl-L-methionine; protein L-glutamate; Tsrglu; Trgglu; Targlu; Tapglu [C]
Specific reaction: S-Adenosyl-L-methionine + protein L-glutamate =S-adenosyl-L-homocysteine + protein L-glutamate methyl ester S-adenosyl-L-methionine + Tsrglu = S-adenosyl-homocysteine + Tsrglu-Me S-adenosyl-L-methionine + Trgglu = S-adenosyl-homocysteine + Trgglu-Me S-ade
General reaction: Methyl group transfer [C]
Inhibitor: A9145C; Ca2+; Methylated receptor protein; S-Adenosyl -L-homocysteine; Sinefungin [C]
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8497190 [H]