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The map label for this gene is shiA [H]

Identifier: 41409325

GI number: 41409325

Start: 3579908

End: 3581320

Strand: Reverse

Name: shiA [H]

Synonym: MAP3227c

Alternate gene names: 41409325

Gene position: 3581320-3579908 (Counterclockwise)

Preceding gene: 41409327

Following gene: 41409324

Centisome position: 74.15

GC content: 70.77

Gene sequence:

>1413_bases
TTGCGGCAAAGCCCCAGCGCATCCGCCCGGCGTTCGCATCACGGCGATGGCAAACATTCGCCGGCGCCGACGGGTTCATT
ACCCTGCAACCACGTGACTCTGACCAGCCCGGCCGCCGCTTCGGCCGGCGACCTTCCCGCCCGGCCGGGGCTGCGCACCG
TCGCTGCGGGCAGCATGATCGGCACCACCATCGAGTGGTACGACTTCTACCTCTACGCGACCGCGTCGGCGCTGGTGTTC
AAACCGCTGTTCTTCCCCAACATCTCGCCGTCCGCCGGGACGCTGGCCTCGTTCGCCACCTACGCCGCCGGATTCGGGGC
CCGCCCGCTCGGTGCGGTGCTGTCCGGCCACTTCGGCGACCGGCTGGGCCGCAAGACCGTGCTGGTGGCCGCGCTGCTGG
TGATGGGTTTGGTCACCACCGCGATCGGGGCGCTGCCGACCTACGCCGAGGCCGGGCTGGCGGCGCCGGCCCTGCTGGCG
TCGCTGCGGGTGGTGCAGGGGCTGGCCGTGGGCGCGGAATGGGGTGGGGCCGCGGTGCTGTCGGTGGAGCATGCGCCGCC
GGGCCGTCGCGGGCTGTTCGGCAGCTTCACCCAGCTGGGCTCCCCGGCCGGCATGCTGCTGGCGACGTCGGTGTTCTTCG
GTGTCCGCAAGGCCACCGGGCCCGCCGCCTTCCTCGGCTTCGGATGGCGAATCCCGTTCCTGCTCAGCATCTTTCTGGTG
GCGGTCGGTCTGTTCGTGCGGCTGCGGCTCACCGACGCCGAAGTCTTCGACCGGCTCAGAAGCCGCGACGAGCTGGCCCG
GCTGCCGATCGTGCAGGTGCTGCGCACCGACGCGCGCAACGTCGTCATCACCACCGGCCTGCGCCTGTCGCAGATCGGGC
TGTTCGTGCTGTTGACCACGTATTCGCTTAGCTACCTTCAGGATTCGTTCGGAAAGGGCAGCGGTGTGGGGCTGGTCGCG
GTGCTGATCTCGTCGGCGCTGGGGTTTCTCAGCACGCCGGGCTGGGCGCTGCTGTCGGACCGGGTCGGGCGGCGGCCGCC
GTACCTGTTCGGCGCGCTGGTGTCGGTCGTGGCGCTGGTGTTGTTCTTCGTCGCCGCCGGCACCGGCTCGGCGGTGCTGG
TGGTCGTGGCAATCGTGTTCGGCGTCAACGTCGTTCACGATGCGATGTACGGTCCGCAGGCCGCCTGGTTCGCCGAACTG
TTCGACACCCGGGTGCGCTACAGCGGATCGAGCCTGGGATATCACATCGGCGCCGTGCTCTCCGGCGGGTTCGCGCCGTT
GATCGCGGCGTCGCTGCTGGTCGCCGGGGGCGGCCGGCCGTGGTTGATCGTCGGCTATTTCGCGGTGCTGGCGGCGATCA
CCGTGGGCGCCGCCTGCGCGGCCCGGGAGACGCGCGGGGAGCCGATCGGATGA

Upstream 100 bases:

>100_bases
CAGCCCGGCAAAGCCGGCGCCGACCACGACGACGTCGACGATCCGCGGCGGCCCTGACACGCGCTCTAGTGAACCGCATT
TCGCGGGGTTTGTCGGCGAA

Downstream 100 bases:

>100_bases
GCGCGGCCCGCCGGATCTACCTCAACGCCTTCGACATGGCCTGCGTCGGGCATCAGTCGGCCGGGCTGTGGCGGCACCCC
GACGACCAGGGCCACCGGTA

Product: hypothetical protein

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MRQSPSASARRSHHGDGKHSPAPTGSLPCNHVTLTSPAAASAGDLPARPGLRTVAAGSMIGTTIEWYDFYLYATASALVF
KPLFFPNISPSAGTLASFATYAAGFGARPLGAVLSGHFGDRLGRKTVLVAALLVMGLVTTAIGALPTYAEAGLAAPALLA
SLRVVQGLAVGAEWGGAAVLSVEHAPPGRRGLFGSFTQLGSPAGMLLATSVFFGVRKATGPAAFLGFGWRIPFLLSIFLV
AVGLFVRLRLTDAEVFDRLRSRDELARLPIVQVLRTDARNVVITTGLRLSQIGLFVLLTTYSLSYLQDSFGKGSGVGLVA
VLISSALGFLSTPGWALLSDRVGRRPPYLFGALVSVVALVLFFVAAGTGSAVLVVVAIVFGVNVVHDAMYGPQAAWFAEL
FDTRVRYSGSSLGYHIGAVLSGGFAPLIAASLLVAGGGRPWLIVGYFAVLAAITVGAACAARETRGEPIG

Sequences:

>Translated_470_residues
MRQSPSASARRSHHGDGKHSPAPTGSLPCNHVTLTSPAAASAGDLPARPGLRTVAAGSMIGTTIEWYDFYLYATASALVF
KPLFFPNISPSAGTLASFATYAAGFGARPLGAVLSGHFGDRLGRKTVLVAALLVMGLVTTAIGALPTYAEAGLAAPALLA
SLRVVQGLAVGAEWGGAAVLSVEHAPPGRRGLFGSFTQLGSPAGMLLATSVFFGVRKATGPAAFLGFGWRIPFLLSIFLV
AVGLFVRLRLTDAEVFDRLRSRDELARLPIVQVLRTDARNVVITTGLRLSQIGLFVLLTTYSLSYLQDSFGKGSGVGLVA
VLISSALGFLSTPGWALLSDRVGRRPPYLFGALVSVVALVLFFVAAGTGSAVLVVVAIVFGVNVVHDAMYGPQAAWFAEL
FDTRVRYSGSSLGYHIGAVLSGGFAPLIAASLLVAGGGRPWLIVGYFAVLAAITVGAACAARETRGEPIG
>Mature_470_residues
MRQSPSASARRSHHGDGKHSPAPTGSLPCNHVTLTSPAAASAGDLPARPGLRTVAAGSMIGTTIEWYDFYLYATASALVF
KPLFFPNISPSAGTLASFATYAAGFGARPLGAVLSGHFGDRLGRKTVLVAALLVMGLVTTAIGALPTYAEAGLAAPALLA
SLRVVQGLAVGAEWGGAAVLSVEHAPPGRRGLFGSFTQLGSPAGMLLATSVFFGVRKATGPAAFLGFGWRIPFLLSIFLV
AVGLFVRLRLTDAEVFDRLRSRDELARLPIVQVLRTDARNVVITTGLRLSQIGLFVLLTTYSLSYLQDSFGKGSGVGLVA
VLISSALGFLSTPGWALLSDRVGRRPPYLFGALVSVVALVLFFVAAGTGSAVLVVVAIVFGVNVVHDAMYGPQAAWFAEL
FDTRVRYSGSSLGYHIGAVLSGGFAPLIAASLLVAGGGRPWLIVGYFAVLAAITVGAACAARETRGEPIG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=390, Percent_Identity=39.7435897435897, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1789941, Length=422, Percent_Identity=39.8104265402844, Blast_Score=245, Evalue=3e-66,
Organism=Escherichia coli, GI1790550, Length=429, Percent_Identity=30.7692307692308, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1788942, Length=386, Percent_Identity=30.8290155440415, Blast_Score=129, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48629; Mature: 48629

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQSPSASARRSHHGDGKHSPAPTGSLPCNHVTLTSPAAASAGDLPARPGLRTVAAGSMI
CCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHCCHHH
GTTIEWYDFYLYATASALVFKPLFFPNISPSAGTLASFATYAAGFGARPLGAVLSGHFGD
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCHHHH
RLGRKTVLVAALLVMGLVTTAIGALPTYAEAGLAAPALLASLRVVQGLAVGAEWGGAAVL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
SVEHAPPGRRGLFGSFTQLGSPAGMLLATSVFFGVRKATGPAAFLGFGWRIPFLLSIFLV
EEECCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHH
AVGLFVRLRLTDAEVFDRLRSRDELARLPIVQVLRTDARNVVITTGLRLSQIGLFVLLTT
HHHHHHHHEECCHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHH
YSLSYLQDSFGKGSGVGLVAVLISSALGFLSTPGWALLSDRVGRRPPYLFGALVSVVALV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHH
LFFVAAGTGSAVLVVVAIVFGVNVVHDAMYGPQAAWFAELFDTRVRYSGSSLGYHIGAVL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
SGGFAPLIAASLLVAGGGRPWLIVGYFAVLAAITVGAACAARETRGEPIG
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRQSPSASARRSHHGDGKHSPAPTGSLPCNHVTLTSPAAASAGDLPARPGLRTVAAGSMI
CCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCHHHHCCHHH
GTTIEWYDFYLYATASALVFKPLFFPNISPSAGTLASFATYAAGFGARPLGAVLSGHFGD
HCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHCHHHH
RLGRKTVLVAALLVMGLVTTAIGALPTYAEAGLAAPALLASLRVVQGLAVGAEWGGAAVL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
SVEHAPPGRRGLFGSFTQLGSPAGMLLATSVFFGVRKATGPAAFLGFGWRIPFLLSIFLV
EEECCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHH
AVGLFVRLRLTDAEVFDRLRSRDELARLPIVQVLRTDARNVVITTGLRLSQIGLFVLLTT
HHHHHHHHEECCHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHH
YSLSYLQDSFGKGSGVGLVAVLISSALGFLSTPGWALLSDRVGRRPPYLFGALVSVVALV
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHH
LFFVAAGTGSAVLVVVAIVFGVNVVHDAMYGPQAAWFAELFDTRVRYSGSSLGYHIGAVL
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHH
SGGFAPLIAASLLVAGGGRPWLIVGYFAVLAAITVGAACAARETRGEPIG
CCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]