BacMap

Definition	Mycobacterium avium subsp. paratuberculosis K-10, complete genome.
Accession	NC_002944
Length	4,829,781

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The map label for this gene is yqhE [C]

Identifier: 41409105

GI number: 41409105

Start: 3344071

End: 3344916

Strand: Direct

Name: yqhE [C]

Synonym: MAP3007

Alternate gene names: 41409105

Gene position: 3344071-3344916 (Clockwise)

Preceding gene: 41409099

Following gene: 41409112

Centisome position: 69.24

GC content: 67.49

Gene sequence:

>846_bases
TTGACTGGCGAGTCGGGCTCCGCCGCACCCTCGATTGCTCTCAACGACGAAAACACGATGCCGGTGCTGGGCCTCGGTGT
CGCGGAGTTGTCGGATGACGAGACCGAACGCGCGGTGTCGGCGGCGCTGGAGGTCGGCTGCCGGCTCATCGACACCGCCG
CGGCCTACGGCAACGAGGCCGCCGTCGGGCGCGCCATCGCCGCCTCGGGGATCCCGCGCGCCGAGCTGTTCGTCACCACC
AAGCTGGCCACCTCCGACCAGGGCTTCAAAGGGGCGATGGACGCCTGCGAGGCCAGCCTGGAGCGGCTCGGGCTGGACTA
CGTCGACCTGTACCTGATTCACTGGCCGGCCCCCGCGCTGGGCACCTACGTGAACTCCTTCGGCGGCATGATCCAGTCCC
GCGGCAACGGCCACGCCCGCTCGATCGGCGTCTCCAACTTCACCGAGGAGTACTTGACGACGGTCATCGACCTCACGTTC
GTCACCCCGGCGGTCAACCAGATCGAGCTGCACCCGCTGCTCAACCAGGAAGCGCTGCGCAAGACCAACGCCGAGCACAA
CGTGGTCACCCAGTCCTATACGCCGCTGGCGCTGGGCAAGCTGAACGACCACCCGACGGTGAACTCGGTGGCCGCCGAAT
ACGGCAAGACCGCGTCGCAGGTGCTGCTGCGGTGGAACCTGCAGTTGAGCAACGCGGTGATCTTCCGGTCGGCCAACGCC
GAGCACATCGCCAGCGACTTCGACGTGTTCGACTTCGAGCTGGCCGCCGAGCACATGGACGCCATCAACGCGCTCAACGA
CGGCACCCGGCTGCGCCCGGACCCAGACACCTACGAGGGCTCCTAA

Upstream 100 bases:

>100_bases
GATTTCGATTTCGGCGCCGAAACGGCGTTGACCTCCTCGCGCGATGACGGCCCCCGGCTCGATGCACACGAACGTTTGAC
TTCACAGGCAGGTGAATGAA

Downstream 100 bases:

>100_bases
CCGCTACCCCGCGGGGCAGGTGGCCGCGGCCTGACGCAGCACACCGGTGGACGCCTGGTCCACCGGCAGCGCATAGCCGC
GCAGCACCGCGATGAACTGC

Product: hypothetical protein

Products: 2-keto-L-gulonate; NADP [C]

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MTGESGSAAPSIALNDENTMPVLGLGVAELSDDETERAVSAALEVGCRLIDTAAAYGNEAAVGRAIAASGIPRAELFVTT
KLATSDQGFKGAMDACEASLERLGLDYVDLYLIHWPAPALGTYVNSFGGMIQSRGNGHARSIGVSNFTEEYLTTVIDLTF
VTPAVNQIELHPLLNQEALRKTNAEHNVVTQSYTPLALGKLNDHPTVNSVAAEYGKTASQVLLRWNLQLSNAVIFRSANA
EHIASDFDVFDFELAAEHMDAINALNDGTRLRPDPDTYEGS

Sequences:

>Translated_281_residues
MTGESGSAAPSIALNDENTMPVLGLGVAELSDDETERAVSAALEVGCRLIDTAAAYGNEAAVGRAIAASGIPRAELFVTT
KLATSDQGFKGAMDACEASLERLGLDYVDLYLIHWPAPALGTYVNSFGGMIQSRGNGHARSIGVSNFTEEYLTTVIDLTF
VTPAVNQIELHPLLNQEALRKTNAEHNVVTQSYTPLALGKLNDHPTVNSVAAEYGKTASQVLLRWNLQLSNAVIFRSANA
EHIASDFDVFDFELAAEHMDAINALNDGTRLRPDPDTYEGS
>Mature_280_residues
TGESGSAAPSIALNDENTMPVLGLGVAELSDDETERAVSAALEVGCRLIDTAAAYGNEAAVGRAIAASGIPRAELFVTTK
LATSDQGFKGAMDACEASLERLGLDYVDLYLIHWPAPALGTYVNSFGGMIQSRGNGHARSIGVSNFTEEYLTTVIDLTFV
TPAVNQIELHPLLNQEALRKTNAEHNVVTQSYTPLALGKLNDHPTVNSVAAEYGKTASQVLLRWNLQLSNAVIFRSANAE
HIASDFDVFDFELAAEHMDAINALNDGTRLRPDPDTYEGS

Specific function: Reduction [C]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI24497577, Length=296, Percent_Identity=32.0945945945946, Blast_Score=151, Evalue=6e-37,
Organism=Homo sapiens, GI5174391, Length=296, Percent_Identity=32.0945945945946, Blast_Score=151, Evalue=6e-37,
Organism=Homo sapiens, GI310109922, Length=287, Percent_Identity=34.4947735191638, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI310109920, Length=305, Percent_Identity=32.7868852459016, Blast_Score=141, Evalue=5e-34,
Organism=Homo sapiens, GI45446745, Length=305, Percent_Identity=32.7868852459016, Blast_Score=141, Evalue=6e-34,
Organism=Homo sapiens, GI4503285, Length=305, Percent_Identity=32.7868852459016, Blast_Score=141, Evalue=6e-34,
Organism=Homo sapiens, GI5453543, Length=305, Percent_Identity=32.7868852459016, Blast_Score=141, Evalue=8e-34,
Organism=Homo sapiens, GI5174695, Length=304, Percent_Identity=31.9078947368421, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI223468663, Length=294, Percent_Identity=30.2721088435374, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI93277124, Length=306, Percent_Identity=31.3725490196078, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI4502049, Length=298, Percent_Identity=33.2214765100671, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI24497585, Length=298, Percent_Identity=31.5436241610738, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI24497583, Length=301, Percent_Identity=31.2292358803987, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI291291012, Length=272, Percent_Identity=29.7794117647059, Blast_Score=121, Evalue=9e-28,
Organism=Homo sapiens, GI300116271, Length=285, Percent_Identity=30.8771929824561, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI300116273, Length=282, Percent_Identity=30.8510638297872, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI207028673, Length=118, Percent_Identity=41.5254237288136, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI310109926, Length=117, Percent_Identity=41.8803418803419, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI310109924, Length=157, Percent_Identity=31.2101910828025, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI87082198, Length=271, Percent_Identity=46.8634686346863, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI1786400, Length=254, Percent_Identity=37.7952755905512, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788081, Length=273, Percent_Identity=28.2051282051282, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71998625, Length=267, Percent_Identity=33.3333333333333, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17552492, Length=276, Percent_Identity=34.0579710144928, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17537075, Length=303, Percent_Identity=32.3432343234323, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17537077, Length=294, Percent_Identity=31.6326530612245, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17564128, Length=299, Percent_Identity=31.1036789297659, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17561298, Length=277, Percent_Identity=29.9638989169675, Blast_Score=134, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17566692, Length=291, Percent_Identity=30.9278350515464, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17537079, Length=301, Percent_Identity=27.5747508305648, Blast_Score=124, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17550248, Length=301, Percent_Identity=32.890365448505, Blast_Score=121, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17538386, Length=273, Percent_Identity=28.5714285714286, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17561300, Length=273, Percent_Identity=29.3040293040293, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17562292, Length=279, Percent_Identity=27.2401433691756, Blast_Score=104, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6320576, Length=280, Percent_Identity=34.6428571428571, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6324694, Length=285, Percent_Identity=33.3333333333333, Blast_Score=149, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6319625, Length=294, Percent_Identity=34.6938775510204, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6322556, Length=270, Percent_Identity=28.8888888888889, Blast_Score=125, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6320079, Length=282, Percent_Identity=29.4326241134752, Blast_Score=122, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6321896, Length=315, Percent_Identity=28.5714285714286, Blast_Score=118, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24663317, Length=296, Percent_Identity=36.1486486486487, Blast_Score=175, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24644950, Length=300, Percent_Identity=35.3333333333333, Blast_Score=163, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24657054, Length=300, Percent_Identity=34.6666666666667, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI45553081, Length=293, Percent_Identity=34.4709897610922, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24662789, Length=299, Percent_Identity=33.1103678929766, Blast_Score=158, Evalue=4e-39,
Organism=Drosophila melanogaster, GI221378297, Length=320, Percent_Identity=32.1875, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI20129731, Length=292, Percent_Identity=36.3013698630137, Blast_Score=157, Evalue=7e-39,
Organism=Drosophila melanogaster, GI21356425, Length=300, Percent_Identity=33, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI281366140, Length=257, Percent_Identity=32.6848249027237, Blast_Score=130, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24662785, Length=300, Percent_Identity=32.6666666666667, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24662781, Length=301, Percent_Identity=31.8936877076412, Blast_Score=119, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: NA

Molecular weight: Translated: 30014; Mature: 29882

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGESGSAAPSIALNDENTMPVLGLGVAELSDDETERAVSAALEVGCRLIDTAAAYGNEA
CCCCCCCCCCEEEECCCCCCCEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
AVGRAIAASGIPRAELFVTTKLATSDQGFKGAMDACEASLERLGLDYVDLYLIHWPAPAL
HHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCHHH
GTYVNSFGGMIQSRGNGHARSIGVSNFTEEYLTTVIDLTFVTPAVNQIELHPLLNQEALR
HHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHH
KTNAEHNVVTQSYTPLALGKLNDHPTVNSVAAEYGKTASQVLLRWNLQLSNAVIFRSANA
HCCCCCCEEECCCCCEEECCCCCCCCHHHHHHHHCCHHHHHHHEECEEECCEEEEECCCH
EHIASDFDVFDFELAAEHMDAINALNDGTRLRPDPDTYEGS
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TGESGSAAPSIALNDENTMPVLGLGVAELSDDETERAVSAALEVGCRLIDTAAAYGNEA
CCCCCCCCCEEEECCCCCCCEEECCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
AVGRAIAASGIPRAELFVTTKLATSDQGFKGAMDACEASLERLGLDYVDLYLIHWPAPAL
HHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCHHH
GTYVNSFGGMIQSRGNGHARSIGVSNFTEEYLTTVIDLTFVTPAVNQIELHPLLNQEALR
HHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHH
KTNAEHNVVTQSYTPLALGKLNDHPTVNSVAAEYGKTASQVLLRWNLQLSNAVIFRSANA
HCCCCCCEEECCCCCEEECCCCCCCCHHHHHHHHCCHHHHHHHEECEEECCEEEEECCCH
EHIASDFDVFDFELAAEHMDAINALNDGTRLRPDPDTYEGS
HHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,5-diketo-D-gluconate; NADPH [C]

Specific reaction: 2,5-diketo-D-gluconate + NADPH = 2-keto-L-gulonate + NADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA