BacMap

Definition	Mycobacterium avium subsp. paratuberculosis K-10, complete genome.
Accession	NC_002944
Length	4,829,781

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The map label for this gene is limC [H]

Identifier: 41408877

GI number: 41408877

Start: 3110842

End: 3111672

Strand: Direct

Name: limC [H]

Synonym: MAP2779

Alternate gene names: 41408877

Gene position: 3110842-3111672 (Clockwise)

Preceding gene: 41408873

Following gene: 41408878

Centisome position: 64.41

GC content: 68.11

Gene sequence:

>831_bases
GTGGGTTCACTAGATGGCAAAGTCGCCTTCATCACCGGGGTGGCGCGCGGCCAGGGCCGCAGCCACGCGGTACGGCTCGC
GCGTGAGGGCGCCAACATCATCGGCATCGACATCTGCGCGGACATCGCCGCCAACGGTTATCCGATGGCCTGCCGCGCGG
AGCTCGACCAGACCGTCGCGCTGGTCGAGGAGGCCGGCGGCAAGATGCTCGGCACGGTGGCCGACGTGCGCGACTTCGGC
CAGGTCAAGGCCGCGCTGGACGCCGGTGTGGAGCAATTCGGGCGGCTGGACATCGTGCTCGCCAACGCCGGCATCGCGCC
GTTGGCCTTCCGGCAGTTGAGCATCGAGGAGGAACTGGCTCAGTGGCGGGCGGTCACCGGCGTCAACCTGGACGGCGCCT
ATCACACGGCGTGGGCGGCCATTCCGCATCTGCTCGCCGGTAACCGCGGCGGGGTGATCATCTTCACCAGTTCGACCGCC
GGCATCAAGGGCTTCGGCGGCCTGCAGGGCGGCGGCCTGGGCTATGCGGCCTCCAAGCACGGCATCGTGGGGCTGATGCG
CACCCTGGCCGATGCGCTTGCGCCGCTGAACATTCGGGTGAACACGGTGCACCCGACCGCGGTCAACACGATGATGGCCA
CCAACGACGACATGATCGAATTCCTTCAGAAGAATCCCGGCGCCGGCCCGCACCTGCAGAACCCGATGCCGGTCGGCATG
CTCGAGCCCGAAGACGTCAGCGCCGCCATCGCCTACCTGGTCTCCGACGAGGCCCGCTACGTCACCGGGGTGACGTTCCC
GGTCGATGCCGGCTTCTGCAACAAGGTCTGA

Upstream 100 bases:

>100_bases
CCCTTTTGCCGTCACGGTAACCATGTTACTGTGCGCGGGTGAGTAACCACGTTACTCACCCCTGAACTGGGCCGCCGGAC
AAGAAACGAGGACGCCCGCC

Downstream 100 bases:

>100_bases
CATGGCAACCGACGGCGCGACGGGCCGGGTCGCCGGCAAACGGGTCCTCATCACCGGCGCCGCGCGGGGCATGGGACGCT
CGCACGCGGTGCGGCTGGCC

Product: short chain dehydrogenase

Products: @DEHY06.txt*3-Dehydro-2- Deoxy-D-Gluconate!; NADH. [C]

Alternate protein names: (4R,6S)-carveol dehydrogenase; CDH; Carveol dehydrogenase [H]

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MGSLDGKVAFITGVARGQGRSHAVRLAREGANIIGIDICADIAANGYPMACRAELDQTVALVEEAGGKMLGTVADVRDFG
QVKAALDAGVEQFGRLDIVLANAGIAPLAFRQLSIEEELAQWRAVTGVNLDGAYHTAWAAIPHLLAGNRGGVIIFTSSTA
GIKGFGGLQGGGLGYAASKHGIVGLMRTLADALAPLNIRVNTVHPTAVNTMMATNDDMIEFLQKNPGAGPHLQNPMPVGM
LEPEDVSAAIAYLVSDEARYVTGVTFPVDAGFCNKV

Sequences:

>Translated_276_residues
MGSLDGKVAFITGVARGQGRSHAVRLAREGANIIGIDICADIAANGYPMACRAELDQTVALVEEAGGKMLGTVADVRDFG
QVKAALDAGVEQFGRLDIVLANAGIAPLAFRQLSIEEELAQWRAVTGVNLDGAYHTAWAAIPHLLAGNRGGVIIFTSSTA
GIKGFGGLQGGGLGYAASKHGIVGLMRTLADALAPLNIRVNTVHPTAVNTMMATNDDMIEFLQKNPGAGPHLQNPMPVGM
LEPEDVSAAIAYLVSDEARYVTGVTFPVDAGFCNKV
>Mature_275_residues
GSLDGKVAFITGVARGQGRSHAVRLAREGANIIGIDICADIAANGYPMACRAELDQTVALVEEAGGKMLGTVADVRDFGQ
VKAALDAGVEQFGRLDIVLANAGIAPLAFRQLSIEEELAQWRAVTGVNLDGAYHTAWAAIPHLLAGNRGGVIIFTSSTAG
IKGFGGLQGGGLGYAASKHGIVGLMRTLADALAPLNIRVNTVHPTAVNTMMATNDDMIEFLQKNPGAGPHLQNPMPVGML
EPEDVSAAIAYLVSDEARYVTGVTFPVDAGFCNKV

Specific function: Catalyzes the oxidation of carveol to carvone, with a strong stereoselectivity since it efficiently converts only the (6S)-stereoisomers, of which (-)-(4R,6S)-trans-carveol is the better substrate. Displays a broad substrate specificity with a preference

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4504505, Length=285, Percent_Identity=31.2280701754386, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI32483357, Length=271, Percent_Identity=27.6752767527675, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI5031737, Length=275, Percent_Identity=29.8181818181818, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI15277342, Length=279, Percent_Identity=31.5412186379928, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI31542939, Length=260, Percent_Identity=28.4615384615385, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI40254992, Length=269, Percent_Identity=30.8550185873606, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI126723750, Length=272, Percent_Identity=26.8382352941176, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI33667109, Length=209, Percent_Identity=30.1435406698565, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI4758504, Length=270, Percent_Identity=29.2592592592593, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI224922801, Length=189, Percent_Identity=30.6878306878307, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI40254986, Length=143, Percent_Identity=32.1678321678322, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI59889578, Length=222, Percent_Identity=30.6306306306306, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI126723191, Length=203, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI7705925, Length=270, Percent_Identity=27.037037037037, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1789208, Length=274, Percent_Identity=31.7518248175182, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI87082100, Length=281, Percent_Identity=32.0284697508897, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787335, Length=271, Percent_Identity=31.3653136531365, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082160, Length=273, Percent_Identity=26.7399267399267, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787905, Length=270, Percent_Identity=28.5185185185185, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI2367175, Length=260, Percent_Identity=31.5384615384615, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788459, Length=274, Percent_Identity=27.3722627737226, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1786812, Length=274, Percent_Identity=27.007299270073, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI2367365, Length=276, Percent_Identity=26.8115942028986, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1789378, Length=272, Percent_Identity=28.3088235294118, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1787526, Length=281, Percent_Identity=25.9786476868327, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1790717, Length=271, Percent_Identity=27.3062730627306, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17568967, Length=275, Percent_Identity=31.6363636363636, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17560676, Length=274, Percent_Identity=27.007299270073, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17561402, Length=264, Percent_Identity=27.2727272727273, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25147288, Length=271, Percent_Identity=30.2583025830258, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71982365, Length=211, Percent_Identity=27.9620853080569, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17562910, Length=283, Percent_Identity=25.7950530035336, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17544670, Length=295, Percent_Identity=25.0847457627119, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI115534694, Length=273, Percent_Identity=27.4725274725275, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17559104, Length=270, Percent_Identity=27.7777777777778, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17562904, Length=214, Percent_Identity=27.5700934579439, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560332, Length=281, Percent_Identity=27.7580071174377, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17564282, Length=213, Percent_Identity=30.0469483568075, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17558628, Length=211, Percent_Identity=32.2274881516588, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17538182, Length=278, Percent_Identity=28.0575539568345, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17531453, Length=279, Percent_Identity=27.2401433691756, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17551412, Length=279, Percent_Identity=27.5985663082437, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17562906, Length=288, Percent_Identity=26.3888888888889, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17562908, Length=288, Percent_Identity=24.3055555555556, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193203117, Length=212, Percent_Identity=27.3584905660377, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193203115, Length=209, Percent_Identity=27.7511961722488, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17538486, Length=285, Percent_Identity=27.3684210526316, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17555706, Length=281, Percent_Identity=27.4021352313167, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=271, Percent_Identity=29.8892988929889, Blast_Score=95, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6324126, Length=279, Percent_Identity=31.1827956989247, Blast_Score=76, Evalue=7e-15,
Organism=Drosophila melanogaster, GI23397609, Length=278, Percent_Identity=32.0143884892086, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI21357041, Length=281, Percent_Identity=30.6049822064057, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24644339, Length=272, Percent_Identity=30.1470588235294, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21355319, Length=264, Percent_Identity=29.1666666666667, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI18859837, Length=207, Percent_Identity=33.8164251207729, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI28571526, Length=279, Percent_Identity=29.7491039426523, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI21357673, Length=269, Percent_Identity=27.8810408921933, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24644337, Length=184, Percent_Identity=32.6086956521739, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI17737361, Length=250, Percent_Identity=29.2, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24639444, Length=280, Percent_Identity=26.4285714285714, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24640492, Length=219, Percent_Identity=25.5707762557078, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364892, Length=253, Percent_Identity=26.8774703557312, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.125 [C]

Molecular weight: Translated: 28680; Mature: 28549

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSLDGKVAFITGVARGQGRSHAVRLAREGANIIGIDICADIAANGYPMACRAELDQTVA
CCCCCCEEEEEEEECCCCCCHHHHHHHHCCCCEEEEEEEHHHHCCCCCEEEHHHHHHHHH
LVEEAGGKMLGTVADVRDFGQVKAALDAGVEQFGRLDIVLANAGIAPLAFRQLSIEEELA
HHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHH
QWRAVTGVNLDGAYHTAWAAIPHLLAGNRGGVIIFTSSTAGIKGFGGLQGGGLGYAASKH
HHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC
GIVGLMRTLADALAPLNIRVNTVHPTAVNTMMATNDDMIEFLQKNPGAGPHLQNPMPVGM
HHHHHHHHHHHHHCCCEEEEEEECCHHHHHEEECCHHHHHHHHCCCCCCCCCCCCCCCCC
LEPEDVSAAIAYLVSDEARYVTGVTFPVDAGFCNKV
CCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCC
>Mature Secondary Structure 
GSLDGKVAFITGVARGQGRSHAVRLAREGANIIGIDICADIAANGYPMACRAELDQTVA
CCCCCEEEEEEEECCCCCCHHHHHHHHCCCCEEEEEEEHHHHCCCCCEEEHHHHHHHHH
LVEEAGGKMLGTVADVRDFGQVKAALDAGVEQFGRLDIVLANAGIAPLAFRQLSIEEELA
HHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHH
QWRAVTGVNLDGAYHTAWAAIPHLLAGNRGGVIIFTSSTAGIKGFGGLQGGGLGYAASKH
HHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCC
GIVGLMRTLADALAPLNIRVNTVHPTAVNTMMATNDDMIEFLQKNPGAGPHLQNPMPVGM
HHHHHHHHHHHHHCCCEEEEEEECCHHHHHEEECCHHHHHHHHCCCCCCCCCCCCCCCCC
LEPEDVSAAIAYLVSDEARYVTGVTFPVDAGFCNKV
CCCCHHHHHHHHHHCCCCEEEEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 15.4 {2-deoxygluconate}} [C]

Substrates: @DEOX03.txt*2-Deoxy-D-Gluconate!; NAD(+) [C]

Specific reaction: @DEOX03.txt*2-Deoxy-D-Gluconate! + NAD(+) = @DEHY06.txt*3-Dehydro-2- Deoxy-D-Gluconate! + NADH. [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10473585 [H]