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The map label for this gene is mcl2 [H]

Identifier: 41407786

GI number: 41407786

Start: 1848428

End: 1849108

Strand: Direct

Name: mcl2 [H]

Synonym: MAP1688

Alternate gene names: 41407786

Gene position: 1848428-1849108 (Clockwise)

Preceding gene: 41407785

Following gene: 41407787

Centisome position: 38.27

GC content: 70.93

Gene sequence:

>681_bases
GTGGTGCGCATCAACGGCCTGGACACCGAGTGGCACGACGACGATCTGGCGGCGGTGGCCGGGTCCGCCGCCGACGGCGT
GCTGGTGCCGAAAGTCGAGACGGCACAACAGGTTCAAGCCCTGGACCGGGCGCTGGACACGTTGGGCGCCGCGGCGTCGC
TGCAGCTGTGGGTGATGATGGAAACACCGCGGGCCTTCCTGCGGGCCGAGGAGGTCGCGTCGGCCAGCGACCGCCTGGCC
GGGCTGGTGGTCGGCACCAATGACCTGGTCAACGAGTTGCACGGCCGGCACGTCGCGGGGCGCGGCCCCGTCGTGCCGGC
GCTGGGCCTGGCGTTGTTGGGGGCGCGGGCGGCCGGAAAGGTCGTCTTGGACGGCGTGTTCAACGACATCACCGACGACG
CGGGTTTCCGGGCCGAGGCGGCCCAGGGCCGGGAGATGGGATTCGACGGCAAGACGCTGATCCACCCGTCCCAGATCGCC
CCGGCCAACGAGCTGTTCGGCCCGTCACAGCAGGAGCTGGCCGACGCCCGCAAGGTGGTGTCGGCCTACCAGCAGGCGCA
GGCCGCGGGAACCAGCGTCATCACCGTCGACGGCCGGATGATCGAGAGTTTGCACGTGCGCGACGCGCAACGGATCCTCG
CGCTCGCCGATCTGATCTCCGAACGGGACGCGGCGTCCTAG

Upstream 100 bases:

>100_bases
TTCGACCTCGAGGACGCCGTCGGGCCCGACGCGAAAGCCGAATCCAGAGCCGCGGTGTGCGAGGCGATCTCGTCGCCGGA
TTACCCGGCCCCGCGAGATC

Downstream 100 bases:

>100_bases
CAGCGCTGCAGCCCGGGCCGGGGCCGCTCGCGTCGGGCACACGGTAGGTTGAACGCGATTCCCAGACCGGAAAGGAGACG
AGGTGGGCCAGACCGCGGCC

Product: hypothetical protein

Products: acetate; oxaloacetate

Alternate protein names: (3S)-malyl-CoA thioesterase [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MVRINGLDTEWHDDDLAAVAGSAADGVLVPKVETAQQVQALDRALDTLGAAASLQLWVMMETPRAFLRAEEVASASDRLA
GLVVGTNDLVNELHGRHVAGRGPVVPALGLALLGARAAGKVVLDGVFNDITDDAGFRAEAAQGREMGFDGKTLIHPSQIA
PANELFGPSQQELADARKVVSAYQQAQAAGTSVITVDGRMIESLHVRDAQRILALADLISERDAAS

Sequences:

>Translated_226_residues
MVRINGLDTEWHDDDLAAVAGSAADGVLVPKVETAQQVQALDRALDTLGAAASLQLWVMMETPRAFLRAEEVASASDRLA
GLVVGTNDLVNELHGRHVAGRGPVVPALGLALLGARAAGKVVLDGVFNDITDDAGFRAEAAQGREMGFDGKTLIHPSQIA
PANELFGPSQQELADARKVVSAYQQAQAAGTSVITVDGRMIESLHVRDAQRILALADLISERDAAS
>Mature_226_residues
MVRINGLDTEWHDDDLAAVAGSAADGVLVPKVETAQQVQALDRALDTLGAAASLQLWVMMETPRAFLRAEEVASASDRLA
GLVVGTNDLVNELHGRHVAGRGPVVPALGLALLGARAAGKVVLDGVFNDITDDAGFRAEAAQGREMGFDGKTLIHPSQIA
PANELFGPSQQELADARKVVSAYQQAQAAGTSVITVDGRMIESLHVRDAQRILALADLISERDAAS

Specific function: Catalyzes the hydrolysis of (3S)-malyl-CoA to (3S)- malate and free CoA. Inactive towards beta-methylmalyl-CoA and other CoA esters [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=235, Percent_Identity=25.9574468085106, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI87081764, Length=223, Percent_Identity=36.322869955157, Blast_Score=124, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17557458, Length=232, Percent_Identity=24.1379310344828, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 23707; Mature: 23707

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRINGLDTEWHDDDLAAVAGSAADGVLVPKVETAQQVQALDRALDTLGAAASLQLWVMM
CEEECCCCCCCCCCHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
ETPRAFLRAEEVASASDRLAGLVVGTNDLVNELHGRHVAGRGPVVPALGLALLGARAAGK
CCCHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCH
VVLDGVFNDITDDAGFRAEAAQGREMGFDGKTLIHPSQIAPANELFGPSQQELADARKVV
HHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCEEECCHHCCCHHHHCCCCHHHHHHHHHHH
SAYQQAQAAGTSVITVDGRMIESLHVRDAQRILALADLISERDAAS
HHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVRINGLDTEWHDDDLAAVAGSAADGVLVPKVETAQQVQALDRALDTLGAAASLQLWVMM
CEEECCCCCCCCCCHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
ETPRAFLRAEEVASASDRLAGLVVGTNDLVNELHGRHVAGRGPVVPALGLALLGARAAGK
CCCHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCH
VVLDGVFNDITDDAGFRAEAAQGREMGFDGKTLIHPSQIAPANELFGPSQQELADARKVV
HHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCEEECCHHCCCHHHHCCCCHHHHHHHHHHH
SAYQQAQAAGTSVITVDGRMIESLHVRDAQRILALADLISERDAAS
HHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0565 {oxaoacetate}} 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA