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The map label for this gene is echA12_2

Identifier: 41407295

GI number: 41407295

Start: 1256730

End: 1257539

Strand: Direct

Name: echA12_2

Synonym: MAP1197

Alternate gene names: 41407295

Gene position: 1256730-1257539 (Clockwise)

Preceding gene: 41407294

Following gene: 41407296

Centisome position: 26.02

GC content: 69.75

Gene sequence:

>810_bases
GTGAGTTTGGTACTGGTAGACCACCCGCGGCCCGGCGTCGCGCTGATCACCCTCAATCGCCCCGAGCGGATGAACTCCAT
GGCGTTCGACGTCATGGTCCCGCTCAAGGAGGCCCTGCAGAAGGTCACCTACGACAACGCGGTGCGGGTGGTCGTGCTGA
CCGGGGCCGGCCGCGGCTTCTCCTCGGGCGCCGACCACAAGTCCGCGGGCACGGTGCCCCACGTCGAGGGCCTGACCCGG
CCCAGCTATGCGCTGCGGTCCATGGAGATCCTCGACGAGGTCATCCTGGCGCTGCGCCGGCTGCATCAACCGGTGATCGC
CGCGGTCAACGGCCCGGCCATCGGCGGCGGCCTGTGCCTGGCGCTGGCCGCCGACATCCGGGTGGCCTCCACCAGCGCCT
ACTTCCGCGCCGCGGGCATCAACAACGGGCTCACCGCCAGCGAGCTGGGCCTGTCCTACCTGCTGCCGCGGGCGATCGGG
TCGTCGCGGGCCTTCGAGATCATGCTGACCGGCCGCGACGTCACCGCCGAAGAGGCCGAGCGGATCGGCCTGGTGTCCTG
CCAGGTGCCCGAGGAGCAACTGCTGGACACCTGCTACGCCATCGCCGCGCGGATCGCGGCGTTCTCCCGGCCGGGAATCG
AGTTGACCAAGCGCACGCTGTGGAGTGGACTGGACGCCGGTAGCCTGGAGGGGCACATGCAAGCCGAGGGCTTGGGACAG
CTTTTCGTCCGCCTGCTCACCGCCAACTTCGAGGAAGCGGTTGCCGCGCGCGCGGAACGACGGCCGCCGGTGTTCACCGA
CGACAAATAG

Upstream 100 bases:

>100_bases
CCCGCCGCTCTGGAAGACCTGGTGCAGCAGGTCCGCGCGTTCGACGTGGACGCGGCCATCGCCGAGCAAGGCGAATAACC
CGACGCGCTACCGTGCACGG

Downstream 100 bases:

>100_bases
TTTTTCAGTTCAGGAGAGCGATCGTGATCACGGCCACGGACCTCGAGGTCCGCGCGGGCGCGCGCATCCTGCTCTCACCC
GACGGGCCCGACCTGCGCGT

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSLVLVDHPRPGVALITLNRPERMNSMAFDVMVPLKEALQKVTYDNAVRVVVLTGAGRGFSSGADHKSAGTVPHVEGLTR
PSYALRSMEILDEVILALRRLHQPVIAAVNGPAIGGGLCLALAADIRVASTSAYFRAAGINNGLTASELGLSYLLPRAIG
SSRAFEIMLTGRDVTAEEAERIGLVSCQVPEEQLLDTCYAIAARIAAFSRPGIELTKRTLWSGLDAGSLEGHMQAEGLGQ
LFVRLLTANFEEAVAARAERRPPVFTDDK

Sequences:

>Translated_269_residues
MSLVLVDHPRPGVALITLNRPERMNSMAFDVMVPLKEALQKVTYDNAVRVVVLTGAGRGFSSGADHKSAGTVPHVEGLTR
PSYALRSMEILDEVILALRRLHQPVIAAVNGPAIGGGLCLALAADIRVASTSAYFRAAGINNGLTASELGLSYLLPRAIG
SSRAFEIMLTGRDVTAEEAERIGLVSCQVPEEQLLDTCYAIAARIAAFSRPGIELTKRTLWSGLDAGSLEGHMQAEGLGQ
LFVRLLTANFEEAVAARAERRPPVFTDDK
>Mature_268_residues
SLVLVDHPRPGVALITLNRPERMNSMAFDVMVPLKEALQKVTYDNAVRVVVLTGAGRGFSSGADHKSAGTVPHVEGLTRP
SYALRSMEILDEVILALRRLHQPVIAAVNGPAIGGGLCLALAADIRVASTSAYFRAAGINNGLTASELGLSYLLPRAIGS
SRAFEIMLTGRDVTAEEAERIGLVSCQVPEEQLLDTCYAIAARIAAFSRPGIELTKRTLWSGLDAGSLEGHMQAEGLGQL
FVRLLTANFEEAVAARAERRPPVFTDDK

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=255, Percent_Identity=32.5490196078431, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI31542718, Length=269, Percent_Identity=30.4832713754647, Blast_Score=104, Evalue=6e-23,
Organism=Homo sapiens, GI70995211, Length=256, Percent_Identity=32.421875, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI68989263, Length=174, Percent_Identity=32.7586206896552, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI45643119, Length=201, Percent_Identity=28.8557213930348, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI260275230, Length=201, Percent_Identity=28.8557213930348, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI260274832, Length=201, Percent_Identity=28.8557213930348, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI116235487, Length=163, Percent_Identity=29.4478527607362, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI20127408, Length=213, Percent_Identity=24.8826291079812, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1787660, Length=268, Percent_Identity=34.3283582089552, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1787659, Length=256, Percent_Identity=31.25, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1788597, Length=180, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI221142681, Length=193, Percent_Identity=33.6787564766839, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788682, Length=210, Percent_Identity=31.4285714285714, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1790281, Length=207, Percent_Identity=29.4685990338164, Blast_Score=68, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=256, Percent_Identity=36.71875, Blast_Score=122, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17554946, Length=257, Percent_Identity=33.0739299610895, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17536985, Length=229, Percent_Identity=32.3144104803493, Blast_Score=100, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=201, Percent_Identity=34.3283582089552, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17560910, Length=180, Percent_Identity=33.8888888888889, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=275, Percent_Identity=27.6363636363636, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17540306, Length=252, Percent_Identity=27.3809523809524, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=184, Percent_Identity=26.6304347826087, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=182, Percent_Identity=27.4725274725275, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI20129971, Length=257, Percent_Identity=34.2412451361868, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24653477, Length=257, Percent_Identity=34.2412451361868, Blast_Score=120, Evalue=8e-28,
Organism=Drosophila melanogaster, GI19920382, Length=272, Percent_Identity=31.25, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=30.6513409961686, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24653139, Length=272, Percent_Identity=31.6176470588235, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24654903, Length=169, Percent_Identity=30.7692307692308, Blast_Score=81, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21357171, Length=223, Percent_Identity=30.4932735426009, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24650670, Length=196, Percent_Identity=32.1428571428571, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28762; Mature: 28630

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLVLVDHPRPGVALITLNRPERMNSMAFDVMVPLKEALQKVTYDNAVRVVVLTGAGRGF
CEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCC
SSGADHKSAGTVPHVEGLTRPSYALRSMEILDEVILALRRLHQPVIAAVNGPAIGGGLCL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCHHHHH
ALAADIRVASTSAYFRAAGINNGLTASELGLSYLLPRAIGSSRAFEIMLTGRDVTAEEAE
HHHHHHEEECCCHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHH
RIGLVSCQVPEEQLLDTCYAIAARIAAFSRPGIELTKRTLWSGLDAGSLEGHMQAEGLGQ
HCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
LFVRLLTANFEEAVAARAERRPPVFTDDK
HHHHHHHCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SLVLVDHPRPGVALITLNRPERMNSMAFDVMVPLKEALQKVTYDNAVRVVVLTGAGRGF
EEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCC
SSGADHKSAGTVPHVEGLTRPSYALRSMEILDEVILALRRLHQPVIAAVNGPAIGGGLCL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCHHHHH
ALAADIRVASTSAYFRAAGINNGLTASELGLSYLLPRAIGSSRAFEIMLTGRDVTAEEAE
HHHHHHEEECCCHHEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHH
RIGLVSCQVPEEQLLDTCYAIAARIAAFSRPGIELTKRTLWSGLDAGSLEGHMQAEGLGQ
HCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHH
LFVRLLTANFEEAVAARAERRPPVFTDDK
HHHHHHHCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]