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The map label for this gene is priA

Identifier: 41407228

GI number: 41407228

Start: 1183797

End: 1185764

Strand: Direct

Name: priA

Synonym: MAP1130

Alternate gene names: 41407228

Gene position: 1183797-1185764 (Clockwise)

Preceding gene: 41407227

Following gene: 41407229

Centisome position: 24.51

GC content: 78.25

Gene sequence:

>1968_bases
ATGCTGTCGGTGCCGCACCTGGACCGCGAATTCGACTATCTGGTGTCGGCCGAGCAATCCGACGACGCCCAGCCGGGCGT
GCGGGTGCGGGTGCGCTTCCACGGCCGGCTGGTCGACGGGTTCCTGCTGGAGCGCCGCCACGACACCGACCACCAGGGCA
AGCTGGGCTGGCTGGATCGGGTGGTGTCGCCTGAGCCGGTGCTGACGGCGGAGATCCGCCGGCTGGTCGACGCGGTGGCG
GCCCGCTACGCGGGGACCCGCCCCGACGTGTTGCGGTTGGCGGTGCCGGCCCGGCACGCCAGGGTGGAGCGGGAGGCCCG
GGCTGCGGCCGGCATACCGATGCCGGCGCCGGTCGACCCGTCCGGCTGGGACGGCTACGGCCGCGGCGCCCAGTTCCTGG
CCGCGCTGGCCGAGTCGCGGGCCGCCCGGGCGGTCTGGCAGGCGCTGCCCGGCGAGCCGTGGACCGACCGATTCGCCGAG
GCCGCCGCCCAGACCGTGCGCACCGGCCGGTCGGTGCTGGCGATCGTCCCCGACCAGCGCGACCTGGATGCGCTGAGCCA
CGCCGTGACGAGCCGCATCGACGCGACCGGCGTGGTCGCCCTGTCGGCCGGGCTGGGCCCGGCCGCCCGGTACCGGCGCT
GGCTGGCGGCGCTGCGCGGCAGCGCGCGGGTGGTGATCGGCACCCGCAGCGCGGTGTTCGCGCCGCTGAGCGACCTGGGC
CTGGTGATGGTGTGGGCCGACGCCGACGACAGCCTGGCCGAGCCGCGGGCGCCCTATCCGCACGCCCGCGAGGTGGCGAT
GCTGCGCGCGCACCAGGCCCGCTGCGCGGCGCTGATCGGCGGTTACGCGCGGACCGCGGAGGCCCAGGCGCTGGTGCGCA
GCGGCTGGGCGCACGACATCGTCGCCGCCCGGCCCGTGGTGCGGGCCCGCACCCCGCGGGTGATCGCGCTCGACGACACG
GGTTACGCCGACGAGCGCGACCCGGCGGCGCGCACCGCGCGCATCCCGTCCGTCGCGCTGCGCGCGGCCCGCTCGGCGCT
GCAGGACGGGGCGCCGGTGCTGGTGCAGGTGCCGCGCCGGGGCTACGTCCCGTCGCTGGCCTGCGCGCGCTGCCGCACCA
TCGCCCGCTGCCGGCACTGCACGGGGCCGCTCTCGCTGGCCGAGTCGCCGGGGGAGGGCGGCGGGGGGCTGGTCTGCCGC
TGGTGCGGGCGCGTCGACCCGACGCGGCGGTGCGCGCGGTGCGGCTCCGACGCGGTGCGCGCCGTCGTCGTCGGCGCCCG
CCGCACCGCCGAGGAGCTCGGCCGCGCGTTTGCGGGCACGACGGTGATCACCTCGTCGGGCGACGCCGTCGTGCCCGAGG
TCGCCGACGGGCCCGCGCTGGTGGTCGCCACGCCGGGGGCCGAACCGCGGGCGCGCGGCGACTACGGGGCGGCGTTGCTG
TTGGACACCTGGGCGCTGCTGGGCCGGCAGGACCTGCGCGCGGCGGAGGACGCGTTGTGGCGCTGGATGACCGCCGCCGC
GCTGGTGCGGCCCCGCGGGGACGGCGGGGTGGTGATGGTGGTCGCCGAATCGTCGCTTCCCACCGTGCAATCGCTGATCC
GCTGGGATCCGGTGGGCCACGCGGAATCCGAGCTGACCGCCCGGGCCGAGGTCGGGCTGCCGCCCAGCGTGCACATGGCC
GCCGTCGACGGAACCCCGGCCGCGGTGAACGCGCTGCTGGATGAGGCCGGACTGACCGGCGGGCAAACCCTGCACGCCGA
CCTGCTCGGGCCGGTGGACCTGCCGCCCGGGGCGCGCCGCCCGGCGGGAACCCCGCCCGGCGCCCCGGTGACGCGGATGC
TGGTGCGGGTGCCGCGCCGCGACGGCCTGGCGCTGGCGGCGGCGCTGCGCCGCGGCGTCGGCGTGCTCAGCGCCCGGCAA
ACCCACGAACCGGCCCGGGTGCAGATCGACCCGCTGCACATCGGCTGA

Upstream 100 bases:

>100_bases
GGAACCTGTTGAGTAGCAACGTGTTTCGCGGCCCGGGGCGGCCGTGAGCCCGGGTCCGCGCACGCCGGTCGAGGTGGAAC
CCATCGCCCGGGTGCTGCCG

Downstream 100 bases:

>100_bases
CCGCCACAAACCGTTAACCTAGGCGATAGTATGCGCATGCAATAATCACTGTTTACGACATTAGAAGGTTGCTACACTTT
CTTTCCTTCGCCGCCGATAG

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 655; Mature: 655

Protein sequence:

>655_residues
MLSVPHLDREFDYLVSAEQSDDAQPGVRVRVRFHGRLVDGFLLERRHDTDHQGKLGWLDRVVSPEPVLTAEIRRLVDAVA
ARYAGTRPDVLRLAVPARHARVEREARAAAGIPMPAPVDPSGWDGYGRGAQFLAALAESRAARAVWQALPGEPWTDRFAE
AAAQTVRTGRSVLAIVPDQRDLDALSHAVTSRIDATGVVALSAGLGPAARYRRWLAALRGSARVVIGTRSAVFAPLSDLG
LVMVWADADDSLAEPRAPYPHAREVAMLRAHQARCAALIGGYARTAEAQALVRSGWAHDIVAARPVVRARTPRVIALDDT
GYADERDPAARTARIPSVALRAARSALQDGAPVLVQVPRRGYVPSLACARCRTIARCRHCTGPLSLAESPGEGGGGLVCR
WCGRVDPTRRCARCGSDAVRAVVVGARRTAEELGRAFAGTTVITSSGDAVVPEVADGPALVVATPGAEPRARGDYGAALL
LDTWALLGRQDLRAAEDALWRWMTAAALVRPRGDGGVVMVVAESSLPTVQSLIRWDPVGHAESELTARAEVGLPPSVHMA
AVDGTPAAVNALLDEAGLTGGQTLHADLLGPVDLPPGARRPAGTPPGAPVTRMLVRVPRRDGLALAAALRRGVGVLSARQ
THEPARVQIDPLHIG

Sequences:

>Translated_655_residues
MLSVPHLDREFDYLVSAEQSDDAQPGVRVRVRFHGRLVDGFLLERRHDTDHQGKLGWLDRVVSPEPVLTAEIRRLVDAVA
ARYAGTRPDVLRLAVPARHARVEREARAAAGIPMPAPVDPSGWDGYGRGAQFLAALAESRAARAVWQALPGEPWTDRFAE
AAAQTVRTGRSVLAIVPDQRDLDALSHAVTSRIDATGVVALSAGLGPAARYRRWLAALRGSARVVIGTRSAVFAPLSDLG
LVMVWADADDSLAEPRAPYPHAREVAMLRAHQARCAALIGGYARTAEAQALVRSGWAHDIVAARPVVRARTPRVIALDDT
GYADERDPAARTARIPSVALRAARSALQDGAPVLVQVPRRGYVPSLACARCRTIARCRHCTGPLSLAESPGEGGGGLVCR
WCGRVDPTRRCARCGSDAVRAVVVGARRTAEELGRAFAGTTVITSSGDAVVPEVADGPALVVATPGAEPRARGDYGAALL
LDTWALLGRQDLRAAEDALWRWMTAAALVRPRGDGGVVMVVAESSLPTVQSLIRWDPVGHAESELTARAEVGLPPSVHMA
AVDGTPAAVNALLDEAGLTGGQTLHADLLGPVDLPPGARRPAGTPPGAPVTRMLVRVPRRDGLALAAALRRGVGVLSARQ
THEPARVQIDPLHIG
>Mature_655_residues
MLSVPHLDREFDYLVSAEQSDDAQPGVRVRVRFHGRLVDGFLLERRHDTDHQGKLGWLDRVVSPEPVLTAEIRRLVDAVA
ARYAGTRPDVLRLAVPARHARVEREARAAAGIPMPAPVDPSGWDGYGRGAQFLAALAESRAARAVWQALPGEPWTDRFAE
AAAQTVRTGRSVLAIVPDQRDLDALSHAVTSRIDATGVVALSAGLGPAARYRRWLAALRGSARVVIGTRSAVFAPLSDLG
LVMVWADADDSLAEPRAPYPHAREVAMLRAHQARCAALIGGYARTAEAQALVRSGWAHDIVAARPVVRARTPRVIALDDT
GYADERDPAARTARIPSVALRAARSALQDGAPVLVQVPRRGYVPSLACARCRTIARCRHCTGPLSLAESPGEGGGGLVCR
WCGRVDPTRRCARCGSDAVRAVVVGARRTAEELGRAFAGTTVITSSGDAVVPEVADGPALVVATPGAEPRARGDYGAALL
LDTWALLGRQDLRAAEDALWRWMTAAALVRPRGDGGVVMVVAESSLPTVQSLIRWDPVGHAESELTARAEVGLPPSVHMA
AVDGTPAAVNALLDEAGLTGGQTLHADLLGPVDLPPGARRPAGTPPGAPVTRMLVRVPRRDGLALAAALRRGVGVLSARQ
THEPARVQIDPLHIG

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the helicase family. PriA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=416, Percent_Identity=26.6826923076923, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 69367; Mature: 69367

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVPHLDREFDYLVSAEQSDDAQPGVRVRVRFHGRLVDGFLLERRHDTDHQGKLGWLDR
CCCCCCCCCCHHHHEECCCCCCCCCCCEEEEEECCEEHHHHHHHCCCCCCCCCCHHHHHH
VVSPEPVLTAEIRRLVDAVAARYAGTRPDVLRLAVPARHARVEREARAAAGIPMPAPVDP
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCC
SGWDGYGRGAQFLAALAESRAARAVWQALPGEPWTDRFAEAAAQTVRTGRSVLAIVPDQR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
DLDALSHAVTSRIDATGVVALSAGLGPAARYRRWLAALRGSARVVIGTRSAVFAPLSDLG
CHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCHHHCCCCCCC
LVMVWADADDSLAEPRAPYPHAREVAMLRAHQARCAALIGGYARTAEAQALVRSGWAHDI
EEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VAARPVVRARTPRVIALDDTGYADERDPAARTARIPSVALRAARSALQDGAPVLVQVPRR
HHHCCHHHCCCCEEEEECCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCC
GYVPSLACARCRTIARCRHCTGPLSLAESPGEGGGGLVCRWCGRVDPTRRCARCGSDAVR
CCCCCHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCEEEEECCCCCHHHHHHHCCCHHHH
AVVVGARRTAEELGRAFAGTTVITSSGDAVVPEVADGPALVVATPGAEPRARGDYGAALL
HHHHCCHHHHHHHHHHHCCCEEEECCCCEECCCCCCCCEEEEECCCCCCCCCCCCCHHHH
LDTWALLGRQDLRAAEDALWRWMTAAALVRPRGDGGVVMVVAESSLPTVQSLIRWDPVGH
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCCHHHHHHHHCCCCCC
AESELTARAEVGLPPSVHMAAVDGTPAAVNALLDEAGLTGGQTLHADLLGPVDLPPGARR
CCHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEHHCCCCCCCCCCCCC
PAGTPPGAPVTRMLVRVPRRDGLALAAALRRGVGVLSARQTHEPARVQIDPLHIG
CCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHCCCCCCEEEEEEEECC
>Mature Secondary Structure
MLSVPHLDREFDYLVSAEQSDDAQPGVRVRVRFHGRLVDGFLLERRHDTDHQGKLGWLDR
CCCCCCCCCCHHHHEECCCCCCCCCCCEEEEEECCEEHHHHHHHCCCCCCCCCCHHHHHH
VVSPEPVLTAEIRRLVDAVAARYAGTRPDVLRLAVPARHARVEREARAAAGIPMPAPVDP
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCC
SGWDGYGRGAQFLAALAESRAARAVWQALPGEPWTDRFAEAAAQTVRTGRSVLAIVPDQR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCC
DLDALSHAVTSRIDATGVVALSAGLGPAARYRRWLAALRGSARVVIGTRSAVFAPLSDLG
CHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCHHHCCCCCCC
LVMVWADADDSLAEPRAPYPHAREVAMLRAHQARCAALIGGYARTAEAQALVRSGWAHDI
EEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VAARPVVRARTPRVIALDDTGYADERDPAARTARIPSVALRAARSALQDGAPVLVQVPRR
HHHCCHHHCCCCEEEEECCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCC
GYVPSLACARCRTIARCRHCTGPLSLAESPGEGGGGLVCRWCGRVDPTRRCARCGSDAVR
CCCCCHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCEEEEECCCCCHHHHHHHCCCHHHH
AVVVGARRTAEELGRAFAGTTVITSSGDAVVPEVADGPALVVATPGAEPRARGDYGAALL
HHHHCCHHHHHHHHHHHCCCEEEECCCCEECCCCCCCCEEEEECCCCCCCCCCCCCHHHH
LDTWALLGRQDLRAAEDALWRWMTAAALVRPRGDGGVVMVVAESSLPTVQSLIRWDPVGH
HHHHHHHCHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCCHHHHHHHHCCCCCC
AESELTARAEVGLPPSVHMAAVDGTPAAVNALLDEAGLTGGQTLHADLLGPVDLPPGARR
CCHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCCEEEHHCCCCCCCCCCCCC
PAGTPPGAPVTRMLVRVPRRDGLALAAALRRGVGVLSARQTHEPARVQIDPLHIG
CCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]