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The map label for this gene is metK

Identifier: 41407224

GI number: 41407224

Start: 1179063

End: 1180274

Strand: Direct

Name: metK

Synonym: MAP1126

Alternate gene names: 41407224

Gene position: 1179063-1180274 (Clockwise)

Preceding gene: 41407223

Following gene: 41407227

Centisome position: 24.41

GC content: 69.31

Gene sequence:

>1212_bases
GTGAGCGAAAAGGGTCGCCTGTTTACCAGTGAGTCGGTGACGGAGGGACATCCCGACAAGATCTGTGACGCGATCAGCGA
CTCGGTGCTCGACGCGCTGCTGGCGCAGGACCCCCGCTCACGTGTCGCGGTCGAGACCCTGGTCACCACCGGGCAGGTGC
ACGTGGTCGGCGAGGTGACGACGACGGCCAAGGAGGCGTTCGCCGACATCACCAACACGGTGCGCGAGCGCATCCTCGAC
ATCGGCTACGACTCCTCGGACAAGGGCTTCGACGGCGCCTCCTGCGGGGTGAACATCGGCATCGGCGCGCAGTCACCCGA
CATCGCCCAGGGCGTTGACACCGCCCACGAGACCCGCGTCGAGGGCGCGGCCGACCCGCTGGACGCCCAGGGCGCCGGCG
ACCAGGGCCTGATGTTCGGCTACGCCATCAAGGACACCCCGGAGATGATGCCGCTGCCGATCGCGCTGGCGCACCGGCTG
TCGCGGCGGCTGACCGAGGTCCGCAAGAGCGGCGTGCTGCCCTACCTGCGTCCGGACGGCAAGACGCAGGTCACCATCGA
ATACGAGGACGACGTTCCCGTCCGGCTGGACACCGTGGTGGTGTCCACCCAGCACGCGGCCGACATCGACCTGGAGAACA
CCCTGACCCCCGACATCCGGGAAAAGGTGCTCAACACCGTGCTCGACGACCTGGCGCACGACACCCTGGACACCTCCTCG
ACGCGGTTGCTGGTCAACCCGACCGGCAAGTTCGTCGTCGGCGGCCCGATGGGCGACGCGGGGCTGACCGGCCGCAAGAT
CATCGTCGACACCTACGGCGGCTGGGCCCGCCACGGCGGCGGCGCCTTCTCCGGCAAGGATCCGTCGAAGGTGGACCGGT
CCGCGGCGTACGCGATGCGCTGGGTGGCCAAGAACATCGTCGCCGCCGGGCTGGCGGAGCGGGTGGAGGTGCAGGTGGCC
TACGCCATCGGCAAGGCCGCCCCGGTGGGCCTGTTCGTGGAGACCTTCGGCACCGCGACCGTCGACCCGGTCAAGATCGA
GAAGATCGTGCCCGAGGTGTTCGACCTGCGGCCCGGCGCGATCATCCGCGACCTGGACCTGCTGCGCCCGATCTACGCGC
AGACCGCCGCCTACGGGCACTTCGGCCGCACCGACGTCGAACTGCCCTGGGAGCAGCTGAACAAGGTCGACGAGCTCAAG
CGCGCCGTCTGA

Upstream 100 bases:

>100_bases
ATAACACGGTCGAGTGCTTCTCGGCGCCGGGACCGCTGTGGGCCGGGCCGACGCCAGCCGATATAATTCGGCTACCTAAC
TATTTGGAAGGATTGGGATT

Downstream 100 bases:

>100_bases
CCCGTCTCGAGCTTCCGCCGAGTCGCGCCGCGTCGCGCCGCCGCGTCGTGCCGAGCGTGAAGCTAGCTTCACGCTCGGCG
CCGAGCGTGAACTTAACTTC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSEKGRLFTSESVTEGHPDKICDAISDSVLDALLAQDPRSRVAVETLVTTGQVHVVGEVTTTAKEAFADITNTVRERILD
IGYDSSDKGFDGASCGVNIGIGAQSPDIAQGVDTAHETRVEGAADPLDAQGAGDQGLMFGYAIKDTPEMMPLPIALAHRL
SRRLTEVRKSGVLPYLRPDGKTQVTIEYEDDVPVRLDTVVVSTQHAADIDLENTLTPDIREKVLNTVLDDLAHDTLDTSS
TRLLVNPTGKFVVGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMRWVAKNIVAAGLAERVEVQVA
YAIGKAAPVGLFVETFGTATVDPVKIEKIVPEVFDLRPGAIIRDLDLLRPIYAQTAAYGHFGRTDVELPWEQLNKVDELK
RAV

Sequences:

>Translated_403_residues
MSEKGRLFTSESVTEGHPDKICDAISDSVLDALLAQDPRSRVAVETLVTTGQVHVVGEVTTTAKEAFADITNTVRERILD
IGYDSSDKGFDGASCGVNIGIGAQSPDIAQGVDTAHETRVEGAADPLDAQGAGDQGLMFGYAIKDTPEMMPLPIALAHRL
SRRLTEVRKSGVLPYLRPDGKTQVTIEYEDDVPVRLDTVVVSTQHAADIDLENTLTPDIREKVLNTVLDDLAHDTLDTSS
TRLLVNPTGKFVVGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMRWVAKNIVAAGLAERVEVQVA
YAIGKAAPVGLFVETFGTATVDPVKIEKIVPEVFDLRPGAIIRDLDLLRPIYAQTAAYGHFGRTDVELPWEQLNKVDELK
RAV
>Mature_402_residues
SEKGRLFTSESVTEGHPDKICDAISDSVLDALLAQDPRSRVAVETLVTTGQVHVVGEVTTTAKEAFADITNTVRERILDI
GYDSSDKGFDGASCGVNIGIGAQSPDIAQGVDTAHETRVEGAADPLDAQGAGDQGLMFGYAIKDTPEMMPLPIALAHRLS
RRLTEVRKSGVLPYLRPDGKTQVTIEYEDDVPVRLDTVVVSTQHAADIDLENTLTPDIREKVLNTVLDDLAHDTLDTSST
RLLVNPTGKFVVGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMRWVAKNIVAAGLAERVEVQVAY
AIGKAAPVGLFVETFGTATVDPVKIEKIVPEVFDLRPGAIIRDLDLLRPIYAQTAAYGHFGRTDVELPWEQLNKVDELKR
AV

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=395, Percent_Identity=54.6835443037975, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI5174529, Length=389, Percent_Identity=54.2416452442159, Blast_Score=377, Evalue=1e-105,
Organism=Escherichia coli, GI1789311, Length=397, Percent_Identity=58.6901763224181, Blast_Score=448, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17538494, Length=396, Percent_Identity=54.040404040404, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25145633, Length=400, Percent_Identity=54.5, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17538498, Length=396, Percent_Identity=53.5353535353535, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17551082, Length=396, Percent_Identity=52.7777777777778, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI32565905, Length=396, Percent_Identity=49.2424242424242, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI25145635, Length=348, Percent_Identity=52.5862068965517, Blast_Score=320, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI32565911, Length=344, Percent_Identity=51.7441860465116, Blast_Score=318, Evalue=4e-87,
Organism=Caenorhabditis elegans, GI32565903, Length=315, Percent_Identity=52.6984126984127, Blast_Score=302, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI32565909, Length=92, Percent_Identity=54.3478260869565, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI32565907, Length=43, Percent_Identity=72.0930232558139, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320710, Length=401, Percent_Identity=55.6109725685786, Blast_Score=401, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6323209, Length=393, Percent_Identity=56.4885496183206, Blast_Score=398, Evalue=1e-112,
Organism=Drosophila melanogaster, GI45552159, Length=390, Percent_Identity=51.5384615384615, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580529, Length=390, Percent_Identity=51.5384615384615, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580537, Length=390, Percent_Identity=51.5384615384615, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580533, Length=390, Percent_Identity=51.5384615384615, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580535, Length=390, Percent_Identity=51.5384615384615, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580531, Length=390, Percent_Identity=51.5384615384615, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580527, Length=390, Percent_Identity=51.2820512820513, Blast_Score=351, Evalue=6e-97,
Organism=Drosophila melanogaster, GI24580525, Length=390, Percent_Identity=51.2820512820513, Blast_Score=351, Evalue=6e-97,
Organism=Drosophila melanogaster, GI24580541, Length=378, Percent_Identity=50, Blast_Score=329, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24580539, Length=119, Percent_Identity=50.4201680672269, Blast_Score=115, Evalue=4e-26,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43257; Mature: 43126

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKGRLFTSESVTEGHPDKICDAISDSVLDALLAQDPRSRVAVETLVTTGQVHVVGEVT
CCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEHH
TTAKEAFADITNTVRERILDIGYDSSDKGFDGASCGVNIGIGAQSPDIAQGVDTAHETRV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHCCHHHHHHC
EGAADPLDAQGAGDQGLMFGYAIKDTPEMMPLPIALAHRLSRRLTEVRKSGVLPYLRPDG
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCC
KTQVTIEYEDDVPVRLDTVVVSTQHAADIDLENTLTPDIREKVLNTVLDDLAHDTLDTSS
CEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
TRLLVNPTGKFVVGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMR
CEEEECCCCCEEECCCCCCCCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHH
WVAKNIVAAGLAERVEVQVAYAIGKAAPVGLFVETFGTATVDPVKIEKIVPEVFDLRPGA
HHHHHHHHHHHHHHHEEEEEEECCCCCCCHHEEECCCCCCCCCHHHHHHHHHHHCCCCCH
IIRDLDLLRPIYAQTAAYGHFGRTDVELPWEQLNKVDELKRAV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEKGRLFTSESVTEGHPDKICDAISDSVLDALLAQDPRSRVAVETLVTTGQVHVVGEVT
CCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEHH
TTAKEAFADITNTVRERILDIGYDSSDKGFDGASCGVNIGIGAQSPDIAQGVDTAHETRV
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHCCHHHHHHC
EGAADPLDAQGAGDQGLMFGYAIKDTPEMMPLPIALAHRLSRRLTEVRKSGVLPYLRPDG
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEECCCC
KTQVTIEYEDDVPVRLDTVVVSTQHAADIDLENTLTPDIREKVLNTVLDDLAHDTLDTSS
CEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
TRLLVNPTGKFVVGGPMGDAGLTGRKIIVDTYGGWARHGGGAFSGKDPSKVDRSAAYAMR
CEEEECCCCCEEECCCCCCCCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHH
WVAKNIVAAGLAERVEVQVAYAIGKAAPVGLFVETFGTATVDPVKIEKIVPEVFDLRPGA
HHHHHHHHHHHHHHHEEEEEEECCCCCCCHHEEECCCCCCCCCHHHHHHHHHHHCCCCCH
IIRDLDLLRPIYAQTAAYGHFGRTDVELPWEQLNKVDELKRAV
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA