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The map label for this gene is ykvY [H]

Identifier: 41406805

GI number: 41406805

Start: 730559

End: 731686

Strand: Direct

Name: ykvY [H]

Synonym: MAP0707

Alternate gene names: 41406805

Gene position: 730559-731686 (Clockwise)

Preceding gene: 41406804

Following gene: 41406806

Centisome position: 15.13

GC content: 73.05

Gene sequence:

>1128_bases
ATGTCAGTTGAAGTCTGCCCCGACGAGCGCACGCTGCGCTCGGGGCGCCGCCGGCGGGCGTTGGACCAGATGGCCGCCCA
CGACCTCGACGTGCTGGTGCTCGGTCGGCAGGCCAACGTTCGCTACGTCTCCGGGGCGCCGCAACTGTGGGTCGCGGGCA
CCCGGCCGTTCGGCCCGACCTGCGTGCTGGTGCGCGAGACCGGCGCGGTTCACCTGCTCAGCACCTGGGACGAGGGCGTG
CCCGAGGACATCCCGCACGAAAACCTCTACGGCATCTCGTGGAACCCGGCCAACACCGTGGCGGCGCTGCGCCGCATCGA
CGGCAGCCAAACCGCCCGGCGCGTCGGAACCGACGCACTCTCACCGGTTTTCGCGCAGCTGCTGCCCACCGTGTTCCCCA
ACGCGCAACTGGTCGACGGAGAACTGGCCATGCGGGCCGCCCGCCGCGTCAAGACCGCCGAGGAGATCGTGGCGCTGCGG
GAATCCATCGCGGTCGCCGAGTCGGCGCTGGCGGCCGCGGTCTCGGAGTTGCGCCCCGGGGTCAGCGAGCAGGCGCTGGC
CGGCGTCCTGCTGGAGGCCTCCGCGGCGGGCGGGGTGAGCACCCCGTCGAATCAGGATGTGGCGTGGGCGACCTCGCGCA
GCCATCCGTGGCGGCGCGCCGACGGTGACGGTCGTATCCAGGACGGCGACCTGGTGGCATTCTCCGCCGGCGTGCTGGCC
GGCGGCTACGTCGGGGAAGTGGGACGCACCTGGCCGGCCGACAAGAAGCACGCCCCCAGCGGCGCCGCGGCCCTGCACCG
GCGCTGGGACGCCTTGTGGGACAAGCTGATCGCTGCCTGCCATCCGGGCGTGCGGGCGGACGAGCTGATGACGGCCTACC
GGGCCGCGGGGGAGCCGGCACCGCCGATGCCGGTGGCCCGCGGACTGGGTATGGGTTTCGACCCGCCGGTGGTCTCCCAG
CACCTGCCCGCGACCGCCGCCGGCGAACGGCTGGAGCCGGGGATGGTGCTCGCCGTGACGGGATACGTGTGGGAGGAAGG
AGTCGGCGCGGTGTTCGGCCGGGAAGCGGTCCTGATCACCGCCGACGGACCCGAAGTGCTCACCGCGAGCCCGTTCTGGC
AGCCCTGA

Upstream 100 bases:

>100_bases
GAGGACGGCACCGGCGGCTACCGCAGCGAAGAGGTTCTGGTGATCACCGAGGAGGGCTGGATCCGCTTGACCGACTACCC
CTATGACCCGTATGGCCGTC

Downstream 100 bases:

>100_bases
CCATGCCCCGCCCCGGCGAAGCGAACGAAGTAGTGACGCAACCCCCGCTGGCCGGCTATACGGTGATCGATCTGTCCACC
GGCATCGCCGGAGCCTACTG

Product: hypothetical protein

Products: N-terminal amino acid including proline [C]

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MSVEVCPDERTLRSGRRRRALDQMAAHDLDVLVLGRQANVRYVSGAPQLWVAGTRPFGPTCVLVRETGAVHLLSTWDEGV
PEDIPHENLYGISWNPANTVAALRRIDGSQTARRVGTDALSPVFAQLLPTVFPNAQLVDGELAMRAARRVKTAEEIVALR
ESIAVAESALAAAVSELRPGVSEQALAGVLLEASAAGGVSTPSNQDVAWATSRSHPWRRADGDGRIQDGDLVAFSAGVLA
GGYVGEVGRTWPADKKHAPSGAAALHRRWDALWDKLIAACHPGVRADELMTAYRAAGEPAPPMPVARGLGMGFDPPVVSQ
HLPATAAGERLEPGMVLAVTGYVWEEGVGAVFGREAVLITADGPEVLTASPFWQP

Sequences:

>Translated_375_residues
MSVEVCPDERTLRSGRRRRALDQMAAHDLDVLVLGRQANVRYVSGAPQLWVAGTRPFGPTCVLVRETGAVHLLSTWDEGV
PEDIPHENLYGISWNPANTVAALRRIDGSQTARRVGTDALSPVFAQLLPTVFPNAQLVDGELAMRAARRVKTAEEIVALR
ESIAVAESALAAAVSELRPGVSEQALAGVLLEASAAGGVSTPSNQDVAWATSRSHPWRRADGDGRIQDGDLVAFSAGVLA
GGYVGEVGRTWPADKKHAPSGAAALHRRWDALWDKLIAACHPGVRADELMTAYRAAGEPAPPMPVARGLGMGFDPPVVSQ
HLPATAAGERLEPGMVLAVTGYVWEEGVGAVFGREAVLITADGPEVLTASPFWQP
>Mature_374_residues
SVEVCPDERTLRSGRRRRALDQMAAHDLDVLVLGRQANVRYVSGAPQLWVAGTRPFGPTCVLVRETGAVHLLSTWDEGVP
EDIPHENLYGISWNPANTVAALRRIDGSQTARRVGTDALSPVFAQLLPTVFPNAQLVDGELAMRAARRVKTAEEIVALRE
SIAVAESALAAAVSELRPGVSEQALAGVLLEASAAGGVSTPSNQDVAWATSRSHPWRRADGDGRIQDGDLVAFSAGVLAG
GYVGEVGRTWPADKKHAPSGAAALHRRWDALWDKLIAACHPGVRADELMTAYRAAGEPAPPMPVARGLGMGFDPPVVSQH
LPATAAGERLEPGMVLAVTGYVWEEGVGAVFGREAVLITADGPEVLTASPFWQP

Specific function: Unknown

COG id: COG0006

COG function: function code E; Xaa-Pro aminopeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24B family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000587
- InterPro:   IPR001714
- InterPro:   IPR000994
- InterPro:   IPR001131 [H]

Pfam domain/function: PF01321 Creatinase_N; PF00557 Peptidase_M24 [H]

EC number: 3.4.11.9 [C]

Molecular weight: Translated: 39750; Mature: 39619

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVEVCPDERTLRSGRRRRALDQMAAHDLDVLVLGRQANVRYVSGAPQLWVAGTRPFGPT
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCEEEEECCCCCCCE
CVLVRETGAVHLLSTWDEGVPEDIPHENLYGISWNPANTVAALRRIDGSQTARRVGTDAL
EEEEECCCCEEEEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCHHHHHHHCHHHH
SPVFAQLLPTVFPNAQLVDGELAMRAARRVKTAEEIVALRESIAVAESALAAAVSELRPG
HHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VSEQALAGVLLEASAAGGVSTPSNQDVAWATSRSHPWRRADGDGRIQDGDLVAFSAGVLA
CCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEECCCEEEEECCHHC
GGYVGEVGRTWPADKKHAPSGAAALHRRWDALWDKLIAACHPGVRADELMTAYRAAGEPA
CCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
PPMPVARGLGMGFDPPVVSQHLPATAAGERLEPGMVLAVTGYVWEEGVGAVFGREAVLIT
CCCHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCEEEEEECHHHHCCCCCEECCCEEEEE
ADGPEVLTASPFWQP
CCCCCEEECCCCCCC
>Mature Secondary Structure 
SVEVCPDERTLRSGRRRRALDQMAAHDLDVLVLGRQANVRYVSGAPQLWVAGTRPFGPT
CCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCCEEEEECCCCCCCE
CVLVRETGAVHLLSTWDEGVPEDIPHENLYGISWNPANTVAALRRIDGSQTARRVGTDAL
EEEEECCCCEEEEECCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCHHHHHHHCHHHH
SPVFAQLLPTVFPNAQLVDGELAMRAARRVKTAEEIVALRESIAVAESALAAAVSELRPG
HHHHHHHHHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VSEQALAGVLLEASAAGGVSTPSNQDVAWATSRSHPWRRADGDGRIQDGDLVAFSAGVLA
CCHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEECCCEEEEECCHHC
GGYVGEVGRTWPADKKHAPSGAAALHRRWDALWDKLIAACHPGVRADELMTAYRAAGEPA
CCCHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
PPMPVARGLGMGFDPPVVSQHLPATAAGERLEPGMVLAVTGYVWEEGVGAVFGREAVLIT
CCCHHHHHCCCCCCCCHHHHCCCCCCCCCCCCCCEEEEEECHHHHCCCCCEECCCEEEEE
ADGPEVLTASPFWQP
CCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Co2+ [C]

Kcat value (1/min): 8100 [C]

Specific activity: 3.45

Km value (mM): 3 {(4-nitro)Phe-Pro-HN-CH2-CH2-NH-o-aminobenzoyl}} 0.22 {(4-nitro)Phe-Pro-Pro-HN-CH2-CH2-NH-o-aminobenzoyl}} [C]

Substrates: N-terminal amino acid including proline [C]

Specific reaction: Release of any N-terminal amino acid including proline that mislinked with proline even from a dipeptide or tripeptide [C]

General reaction: carboxylic acid amide hydrolysis [C]

Inhibitor: thiazolidide acid; -2-hydroxy -3-amino acidpyrrolidide acid; Pro-methyl ester acid; Pro-Phe-methyl ester acid; 2-hydroxy -3-aminoacyl-Pro-OH dipeptides [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]