BacMap

Definition	Mycobacterium avium subsp. paratuberculosis K-10, complete genome.
Accession	NC_002944
Length	4,829,781

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The map label for this gene is fabG2 [H]

Identifier: 41406634

GI number: 41406634

Start: 562011

End: 562793

Strand: Direct

Name: fabG2 [H]

Synonym: MAP0536

Alternate gene names: 41406634

Gene position: 562011-562793 (Clockwise)

Preceding gene: 41406633

Following gene: 41406635

Centisome position: 11.64

GC content: 65.77

Gene sequence:

>783_bases
ATGGCAGGGATGCTCGACGGCAAGGTGGTGGTCATCAGCGGCGTGGGGCCGGGGCTGGGCACCACGCTGGCGCACCGATG
CGCCGACAGCGGGGCCGACCTGGTGCTGGCGGCGCGTACCGCCGAGCGGCTGGAGAAGGTGGCCAAGGAGGTCAACGACG
GCGGCCACCGGGCGCTGGCGGTGCGCACCGACATCACCGACGACGACGAAGTCGCCTACCTGGTCGAGACCACCATGGCG
ACCTACGGCAGGGCCGACGTGCTGATCAACAACGCATTCCGGGTGCCGTCGATGAAACGGTTGGCGGGAACCAGTTTTCA
GCACATCCGCGACGCGATCGAGTTGAGCGCGCTGGGCGCGCTGCGGCTGATCCAGGCCTTCACACCCGCACTGGAGACGG
CGCACGGATCCATCGTCAACGTCAACTCCATGGTGCTGCGTCATTCGCAGGCCAAGTACGGTGCGTACAAGATGGCCAAG
TCGGCGCTGCTGTCCATGTCGCAGTCGCTGGCCACCGAGCTGGGCGAAAAGGGCATCCGGGTCAATTCGGTTGCGCCGGG
CTACATCTGGGGCGATACCTTGCAGGCCTACTTCGAGCACCAGGCGGGCAAGTACGGCACCACGGTCGAGCAGATCTACG
CGGCCACCGCCGCCAACTCCGACCTCAAACGCCTGCCCACCGAGGACGAAGTCGCTTCGGCCATCATGTTTTTGGCCAGT
GACCTGTCCAGCGGCATCACCGGGCAGACCTTGGACGTCAACTGCGGGGAATACCACACGTGA

Upstream 100 bases:

>100_bases
AGCTGCCCTACTACGAGCACAACAAGATCTCCGACGACGAATGGCGGGCGCGAATCGCGTTGCGTCAACAACAGATTGCG
GCAAGGATGCTGGGGTGACG

Downstream 100 bases:

>100_bases
GCGATCGCACCGACATCGGCACCGTCGAGGAGCTGCACGCATCGGCCACCAAGCTGACCGGGCTCGACGACTTCGGCACC
GACGACGACAACTACCTGCA

Product: short chain dehydrogenase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MAGMLDGKVVVISGVGPGLGTTLAHRCADSGADLVLAARTAERLEKVAKEVNDGGHRALAVRTDITDDDEVAYLVETTMA
TYGRADVLINNAFRVPSMKRLAGTSFQHIRDAIELSALGALRLIQAFTPALETAHGSIVNVNSMVLRHSQAKYGAYKMAK
SALLSMSQSLATELGEKGIRVNSVAPGYIWGDTLQAYFEHQAGKYGTTVEQIYAATAANSDLKRLPTEDEVASAIMFLAS
DLSSGITGQTLDVNCGEYHT

Sequences:

>Translated_260_residues
MAGMLDGKVVVISGVGPGLGTTLAHRCADSGADLVLAARTAERLEKVAKEVNDGGHRALAVRTDITDDDEVAYLVETTMA
TYGRADVLINNAFRVPSMKRLAGTSFQHIRDAIELSALGALRLIQAFTPALETAHGSIVNVNSMVLRHSQAKYGAYKMAK
SALLSMSQSLATELGEKGIRVNSVAPGYIWGDTLQAYFEHQAGKYGTTVEQIYAATAANSDLKRLPTEDEVASAIMFLAS
DLSSGITGQTLDVNCGEYHT
>Mature_259_residues
AGMLDGKVVVISGVGPGLGTTLAHRCADSGADLVLAARTAERLEKVAKEVNDGGHRALAVRTDITDDDEVAYLVETTMAT
YGRADVLINNAFRVPSMKRLAGTSFQHIRDAIELSALGALRLIQAFTPALETAHGSIVNVNSMVLRHSQAKYGAYKMAKS
ALLSMSQSLATELGEKGIRVNSVAPGYIWGDTLQAYFEHQAGKYGTTVEQIYAATAANSDLKRLPTEDEVASAIMFLASD
LSSGITGQTLDVNCGEYHT

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI19923817, Length=269, Percent_Identity=27.8810408921933, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI59889578, Length=184, Percent_Identity=32.0652173913043, Blast_Score=98, Evalue=6e-21,
Organism=Homo sapiens, GI10190704, Length=262, Percent_Identity=29.0076335877863, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI5031737, Length=251, Percent_Identity=24.7011952191235, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI32483357, Length=253, Percent_Identity=24.5059288537549, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI33667109, Length=190, Percent_Identity=27.3684210526316, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI7706318, Length=186, Percent_Identity=32.258064516129, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI7705925, Length=265, Percent_Identity=29.0566037735849, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI40254992, Length=250, Percent_Identity=29.2, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI15277342, Length=264, Percent_Identity=25.7575757575758, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI20149619, Length=192, Percent_Identity=27.6041666666667, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI4503301, Length=232, Percent_Identity=26.7241379310345, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787905, Length=260, Percent_Identity=30, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787335, Length=258, Percent_Identity=31.0077519379845, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI87082100, Length=253, Percent_Identity=31.2252964426877, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790717, Length=255, Percent_Identity=27.843137254902, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789208, Length=255, Percent_Identity=27.0588235294118, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789378, Length=259, Percent_Identity=25.0965250965251, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787526, Length=258, Percent_Identity=25.5813953488372, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1788459, Length=252, Percent_Identity=28.1746031746032, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789057, Length=255, Percent_Identity=24.7058823529412, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1787820, Length=247, Percent_Identity=23.4817813765182, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI2367175, Length=218, Percent_Identity=24.7706422018349, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1786812, Length=263, Percent_Identity=28.1368821292776, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI87082160, Length=259, Percent_Identity=24.7104247104247, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17560676, Length=256, Percent_Identity=26.953125, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17555706, Length=255, Percent_Identity=29.0196078431373, Blast_Score=97, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17544670, Length=278, Percent_Identity=30.5755395683453, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17562904, Length=266, Percent_Identity=29.3233082706767, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17551412, Length=270, Percent_Identity=30, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17562910, Length=262, Percent_Identity=28.6259541984733, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17538480, Length=269, Percent_Identity=28.996282527881, Blast_Score=90, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI71994600, Length=253, Percent_Identity=30.4347826086957, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17531453, Length=265, Percent_Identity=30.188679245283, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17561402, Length=256, Percent_Identity=26.171875, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17559104, Length=260, Percent_Identity=28.4615384615385, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17562908, Length=271, Percent_Identity=30.9963099630996, Blast_Score=87, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17536025, Length=266, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI193204405, Length=263, Percent_Identity=27.7566539923954, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17560150, Length=262, Percent_Identity=28.2442748091603, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17562906, Length=264, Percent_Identity=29.1666666666667, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17560332, Length=189, Percent_Identity=31.7460317460317, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17562990, Length=262, Percent_Identity=29.0076335877863, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17565030, Length=270, Percent_Identity=28.5185185185185, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17560220, Length=263, Percent_Identity=28.1368821292776, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994604, Length=252, Percent_Identity=27.3809523809524, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17536651, Length=266, Percent_Identity=26.6917293233083, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25147288, Length=260, Percent_Identity=26.1538461538462, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17538486, Length=196, Percent_Identity=30.1020408163265, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI115534694, Length=253, Percent_Identity=26.0869565217391, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI72000259, Length=261, Percent_Identity=27.5862068965517, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17538182, Length=196, Percent_Identity=29.0816326530612, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17568967, Length=198, Percent_Identity=28.7878787878788, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71982365, Length=234, Percent_Identity=26.4957264957265, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17561272, Length=266, Percent_Identity=27.8195488721804, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17508895, Length=186, Percent_Identity=25.8064516129032, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71997402, Length=189, Percent_Identity=29.1005291005291, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17563726, Length=197, Percent_Identity=28.4263959390863, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI115534660, Length=187, Percent_Identity=26.2032085561497, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=194, Percent_Identity=28.3505154639175, Blast_Score=72, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6324126, Length=262, Percent_Identity=24.8091603053435, Blast_Score=69, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6322226, Length=208, Percent_Identity=25.4807692307692, Blast_Score=69, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6322861, Length=203, Percent_Identity=30.5418719211823, Blast_Score=67, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571526, Length=256, Percent_Identity=33.59375, Blast_Score=110, Evalue=7e-25,
Organism=Drosophila melanogaster, GI21357041, Length=252, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24644339, Length=253, Percent_Identity=32.0158102766798, Blast_Score=97, Evalue=7e-21,
Organism=Drosophila melanogaster, GI23397609, Length=255, Percent_Identity=29.0196078431373, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24644337, Length=258, Percent_Identity=34.8837209302326, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21355319, Length=248, Percent_Identity=25, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24639444, Length=257, Percent_Identity=25.2918287937743, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21358495, Length=190, Percent_Identity=29.4736842105263, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17737361, Length=250, Percent_Identity=24.4, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI21357673, Length=195, Percent_Identity=29.2307692307692, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24643142, Length=253, Percent_Identity=28.8537549407115, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24640492, Length=179, Percent_Identity=28.4916201117318, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24580925, Length=216, Percent_Identity=31.0185185185185, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 27638; Mature: 27506

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGMLDGKVVVISGVGPGLGTTLAHRCADSGADLVLAARTAERLEKVAKEVNDGGHRALA
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCCEEEE
VRTDITDDDEVAYLVETTMATYGRADVLINNAFRVPSMKRLAGTSFQHIRDAIELSALGA
EEECCCCCCCHHHHHHHHHHHCCCHHEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHH
LRLIQAFTPALETAHGSIVNVNSMVLRHSQAKYGAYKMAKSALLSMSQSLATELGEKGIR
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
VNSVAPGYIWGDTLQAYFEHQAGKYGTTVEQIYAATAANSDLKRLPTEDEVASAIMFLAS
ECCCCCCCEEHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHH
DLSSGITGQTLDVNCGEYHT
HHHCCCCCCEEECCCCCCCC
>Mature Secondary Structure 
AGMLDGKVVVISGVGPGLGTTLAHRCADSGADLVLAARTAERLEKVAKEVNDGGHRALA
CCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHCCCCCEEEE
VRTDITDDDEVAYLVETTMATYGRADVLINNAFRVPSMKRLAGTSFQHIRDAIELSALGA
EEECCCCCCCHHHHHHHHHHHCCCHHEEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHH
LRLIQAFTPALETAHGSIVNVNSMVLRHSQAKYGAYKMAKSALLSMSQSLATELGEKGIR
HHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEE
VNSVAPGYIWGDTLQAYFEHQAGKYGTTVEQIYAATAANSDLKRLPTEDEVASAIMFLAS
ECCCCCCCEEHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHH
DLSSGITGQTLDVNCGEYHT
HHHCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.085 {glycochenodeoxycholic} 0.24 {taurochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]