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The map label for this gene is hisJ [H]

Identifier: 39998495

GI number: 39998495

Start: 3747981

End: 3748736

Strand: Reverse

Name: hisJ [H]

Synonym: GSU3406

Alternate gene names: 39998495

Gene position: 3748736-3747981 (Counterclockwise)

Preceding gene: 39998500

Following gene: 39998494

Centisome position: 98.29

GC content: 58.6

Gene sequence:

>756_bases
ATGAACCTCGTGCGTCGTACCCTGATGGTACTCACGCTGGCCGCAGTGTGTCTGACGGCCGCAGTTCCCCGGCAGGCCGA
GGCCGCGGGCCGTACGGTAACCGTTGCCACGGATGCCACCTGGCCGCCCATGGAAATGGTTGACGCCAACAAGAAGATTG
TCGGCTTCGACATCGATTTCATGAACGCCATCGCCAAGGAAGCGGGTTTCGCCGTCCAGTTCAAAAACACCGCCTGGGAC
GGCATCTTCGCGGGGCTCGGCGCAGGCCGTTATGACGCCATCATTTCCTCCGTCACCATCACCGAAGAGCGGAAAAAGCA
GTATGATTTCTCCGACCCCTACATCAATATCGGCCAGATTCTCGTGGTGCCCAAGACCGAGAAGGCGACAAACCTCTCCG
GCCTCAAGGGGAAGAAGGTGGGCGCCCAGATCGGCACCACCGGCGCCTTTGAAATCAAGAAGGTGCAGGGCGTCGAGCTC
AAGAACTATGACGAAATCGGCCTGGCCTTCGAGGATATGGCCGCCGGCCGCATCGTCGGCGTGGTCTGCGACGAGCCTAC
CGCGGCCCACTATGCTCTCCAGAAGGCCGAGTACAAGAACAAGTTCAAGATCGTGGGCAAGTCCTTTACCGCAGAGTCCT
ACGGCATCGTGGTGAAAAAGGGCGACAAGGAATTGCTCGCTCTGATCAACAAGGGCATCAAGGCCGTCAAGGCCAAGAAA
GTTGACGAGCAACTCCGGAAGAAGTGGCTCAGATAA

Upstream 100 bases:

>100_bases
TCCGGCAAAAAGCCATTGACATCAGGAGAAAATAATCATAAAAGTAGCCTCGTTTAGGCGCAGTAAACATTATTGAATGT
CAGTAACCAAGGGAGGAATT

Downstream 100 bases:

>100_bases
ATGGTTGATCAGGACAGGGTACGGCCGGCGGTTGTCGAGGTCGGTGACGGCGCAGCTATTCCGAGCAAAAGCGACATCGG
GCTTTTCACTGCCTGGCGCA

Product: amino acid ABC transporter periplasmic amino acid-binding protein

Products: NA

Alternate protein names: HBP [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MNLVRRTLMVLTLAAVCLTAAVPRQAEAAGRTVTVATDATWPPMEMVDANKKIVGFDIDFMNAIAKEAGFAVQFKNTAWD
GIFAGLGAGRYDAIISSVTITEERKKQYDFSDPYINIGQILVVPKTEKATNLSGLKGKKVGAQIGTTGAFEIKKVQGVEL
KNYDEIGLAFEDMAAGRIVGVVCDEPTAAHYALQKAEYKNKFKIVGKSFTAESYGIVVKKGDKELLALINKGIKAVKAKK
VDEQLRKKWLR

Sequences:

>Translated_251_residues
MNLVRRTLMVLTLAAVCLTAAVPRQAEAAGRTVTVATDATWPPMEMVDANKKIVGFDIDFMNAIAKEAGFAVQFKNTAWD
GIFAGLGAGRYDAIISSVTITEERKKQYDFSDPYINIGQILVVPKTEKATNLSGLKGKKVGAQIGTTGAFEIKKVQGVEL
KNYDEIGLAFEDMAAGRIVGVVCDEPTAAHYALQKAEYKNKFKIVGKSFTAESYGIVVKKGDKELLALINKGIKAVKAKK
VDEQLRKKWLR
>Mature_251_residues
MNLVRRTLMVLTLAAVCLTAAVPRQAEAAGRTVTVATDATWPPMEMVDANKKIVGFDIDFMNAIAKEAGFAVQFKNTAWD
GIFAGLGAGRYDAIISSVTITEERKKQYDFSDPYINIGQILVVPKTEKATNLSGLKGKKVGAQIGTTGAFEIKKVQGVEL
KNYDEIGLAFEDMAAGRIVGVVCDEPTAAHYALQKAEYKNKFKIVGKSFTAESYGIVVKKGDKELLALINKGIKAVKAKK
VDEQLRKKWLR

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=222, Percent_Identity=32.4324324324324, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1787088, Length=254, Percent_Identity=33.0708661417323, Blast_Score=122, Evalue=2e-29,
Organism=Escherichia coli, GI1787085, Length=243, Percent_Identity=33.3333333333333, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1787031, Length=248, Percent_Identity=31.4516129032258, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788649, Length=256, Percent_Identity=30.46875, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788648, Length=229, Percent_Identity=29.6943231441048, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1786876, Length=221, Percent_Identity=26.6968325791855, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 27365; Mature: 27365

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLVRRTLMVLTLAAVCLTAAVPRQAEAAGRTVTVATDATWPPMEMVDANKKIVGFDIDF
CCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEEECCCCCCHHHHCCCCEEEEECHHH
MNAIAKEAGFAVQFKNTAWDGIFAGLGAGRYDAIISSVTITEERKKQYDFSDPYINIGQI
HHHHHHHCCCEEEECCCCCHHHHHHCCCCHHHHHHHHEEECHHHHHCCCCCCCCEECCEE
LVVPKTEKATNLSGLKGKKVGAQIGTTGAFEIKKVQGVELKNYDEIGLAFEDMAAGRIVG
EEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHCCCHHHCCCCEEEE
VVCDEPTAAHYALQKAEYKNKFKIVGKSFTAESYGIVVKKGDKELLALINKGIKAVKAKK
EEECCCCHHHHHHHHHHHCCEEEEEECEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
VDEQLRKKWLR
HHHHHHHHHCC
>Mature Secondary Structure
MNLVRRTLMVLTLAAVCLTAAVPRQAEAAGRTVTVATDATWPPMEMVDANKKIVGFDIDF
CCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCEEEEEECCCCCCHHHHCCCCEEEEECHHH
MNAIAKEAGFAVQFKNTAWDGIFAGLGAGRYDAIISSVTITEERKKQYDFSDPYINIGQI
HHHHHHHCCCEEEECCCCCHHHHHHCCCCHHHHHHHHEEECHHHHHCCCCCCCCEECCEE
LVVPKTEKATNLSGLKGKKVGAQIGTTGAFEIKKVQGVELKNYDEIGLAFEDMAAGRIVG
EEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHCCCHHHCCCCEEEE
VVCDEPTAAHYALQKAEYKNKFKIVGKSFTAESYGIVVKKGDKELLALINKGIKAVKAKK
EEECCCCHHHHHHHHHHHCCEEEEEECEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
VDEQLRKKWLR
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1448614 [H]