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The map label for this gene is pcrA [H]

Identifier: 39998500

GI number: 39998500

Start: 3752116

End: 3754335

Strand: Reverse

Name: pcrA [H]

Synonym: GSU3411

Alternate gene names: 39998500

Gene position: 3754335-3752116 (Counterclockwise)

Preceding gene: 39998502

Following gene: 39998495

Centisome position: 98.43

GC content: 65.05

Gene sequence:

>2220_bases
ATGAAATTGCTCGATAATCTTAACCCTCCCCAGCGGGCCGCGGTTCTTCACGGCGAAGGACCGCTCCTGGTCCTGGCCGG
TGCCGGTTCCGGCAAGACCAGGGTCATCGTCCACCGGATCGCTCACCTGATCCGCGAGCGGGGTGTCCCCGCCCGGCAGA
TTCTTGCCGTGACCTTCACCAACAAGGCCGCCGGCGAGATGCGCGAACGGGTGGAAAAGCTCGTGGGGGGTGAGGTTCCC
CTGATCGCCACCTTTCACTCCACCTGCGCGCGGATCCTTCGCCGGGAGATTCACCACCTGGGCTACGATTCCTCTTTTGC
CATCTATGATGACAAGGACTCCGGCAAGCTCCTCAAGGAAGTGATGGCCGGTCTCGGTCTCGACGAGAAGCGTTTCCCTG
CGACCTCGCTTGCCTCGGCCATCGACGACTGCAAGAACCGGGGGCTTGCGCCGGAGGACCTGCCAGCCGACACCTTCACC
GGCGAGGTGGTGGGCCGCGTCTACGCCGCCTACCAGGAGCGGTTGAAGAAGTGCAACGCCCTCGACTTCGGCGACCTCAT
CATGCTGACCGTCCGCCTCTTCGAGGAGCACCCCGTGGTGCTTGAGCGCTACCGGGACCAGTGGCGCTGGATCCTGGTGG
ACGAATACCAGGATACCAACCCGATCCAGTACCGGCTCGTGCGCCTATTGGCCGGTGAGCACCGCAACCTCTGCGTGGTG
GGTGACGATGACCAATCCATCTATCGCTGGCGCGGGGCCGACATCCGTAACATCCTCGACTTCGAGAAGGACTTCCCCGG
CGTCACCACCATCAAGCTCGAGCAGAACTACCGCTCCACCCGGAACATCCTTGCGGCGGCGGGAGCCGTGGTGGCACGCA
ACCGGGGTCGCAAGGCCAAGACCCTCTGGAGCGAGAACCCGCCGGGCGACCCTATCGTTTACCGCCGTCTGGCCGACGAG
CGGGATGAGGCCCGCTTCGTCTGCCGTGAGATCGAGCGCTACGTACGCCGTGGCGGCGACCTCCGGGACGTGGCGGTCTT
CTACCGCACCAATGCCCAGTCCCGGGTGGTGGAAGACGCTCTGGTGGCCGACGGCATCCCCTACCACATGGTGGGCGGCA
TGCGGTTCTATGAGCGTATGGAGGTAAAAGACGTCCTTGCCTATCTGCGGGTCCTGGCGAACCCGGCTGACGAGGTTTCG
CTCAAGCGGATCATCAACGTTCCCGCTCGGGGGATCGGTCCCGGCACCGTGGACAAGATTTCCGAGCTGGCCGTCCGCCG
GGGGATCACCGTCCTCGACGCCCTGGGTGAGGCGGCCACGGGCGGACTCCTCTCCTCGGCACCCCGGGGTAAGGTGGCTG
CCTTTGCAGCAGTCATGGAGAGGTTTTTCGCCCTGCGCGATCAACTCCCCCTGGCAGAACTCACCACCCGCATCATCGAA
GAGTCAGGCTACGCGGACCGGCTTCGGCAGGAGCGGACCGACGAGGCCGCGGACCGGCTCGCCAACCTTCAGGAACTCGT
GGCTGCCATGGAAGAGTTCGAGCGGACCAGCGATGAGCGGGGACTTGCTCCGTTCCTGGAACAGGTGGCCCTCGTCTCGG
ATCTGGAGCGTAGCGGCACGGGCCGCGAGTCGGCAACGCTTATGACCCTGCACGCGGCAAAGGGGCTCGAGTTTCCCATG
GTTTTCATGATCGGCATGGAGGAGAAGCTCTTTCCCCATGTCCGCTCCCTGGACGACCCCGAGGCCATGGAGGAGGAGCG
CCGGCTCTGCTACGTGGGGATGACCCGGGCACGGGAGCGGCTCTGCCTCACCAATGCCCGGCGCCGGCGGATCTTCGGCC
AGGACCAGGTGAACATGCCCTCCCGCTTCATCGCCGACATCCCCCGGAACCTCCTGGACCTGGAGGATGAATGGCAGCAA
CCCGCCCTCGGCTCCGTCGCGCCCGACACCGCCGGGCGGGGGCGGGAGCCCGCGCGTCACAACCTGGCCTCCATCCTCGA
AACCGAGATCGAGCCGGATTTCGCCGACGTGGAGATCGTCCCCGTCGAACCCGAAGAAAGCGGCGTCTGGCTCGGCATGA
AGGTACGGCACGGAAAGTTCGGTGTCGGCACCATCCGGAAGATTGAAGGGCAGGGGGATGAGCAGAAGGTGATCGTCTGG
TTCAACTCGGTGGGGCCGAAGAAGCTTCTGGTCCGCTTTGCCGGTCTGGAGAGGGTCTAG

Upstream 100 bases:

>100_bases
GGTTGAAGGGGGTGAGCCGTTTTCGGTATAGTCGACTGTCACCCCGGTCAGCCATCCTTTCCCTTCCGGCGGTGGCGAAT
TCGACCTCCCGAAGGCGCCC

Downstream 100 bases:

>100_bases
CATCCGGATTAGTTGATTTTTACTCGTAAATTTGGTTCTTCTGGCTGCCGCAGGTGTTGCCCCGTCTGGTTAGGACCCGC
TCTGCCGGTCTTGCCCGAGC

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 739; Mature: 739

Protein sequence:

>739_residues
MKLLDNLNPPQRAAVLHGEGPLLVLAGAGSGKTRVIVHRIAHLIRERGVPARQILAVTFTNKAAGEMRERVEKLVGGEVP
LIATFHSTCARILRREIHHLGYDSSFAIYDDKDSGKLLKEVMAGLGLDEKRFPATSLASAIDDCKNRGLAPEDLPADTFT
GEVVGRVYAAYQERLKKCNALDFGDLIMLTVRLFEEHPVVLERYRDQWRWILVDEYQDTNPIQYRLVRLLAGEHRNLCVV
GDDDQSIYRWRGADIRNILDFEKDFPGVTTIKLEQNYRSTRNILAAAGAVVARNRGRKAKTLWSENPPGDPIVYRRLADE
RDEARFVCREIERYVRRGGDLRDVAVFYRTNAQSRVVEDALVADGIPYHMVGGMRFYERMEVKDVLAYLRVLANPADEVS
LKRIINVPARGIGPGTVDKISELAVRRGITVLDALGEAATGGLLSSAPRGKVAAFAAVMERFFALRDQLPLAELTTRIIE
ESGYADRLRQERTDEAADRLANLQELVAAMEEFERTSDERGLAPFLEQVALVSDLERSGTGRESATLMTLHAAKGLEFPM
VFMIGMEEKLFPHVRSLDDPEAMEEERRLCYVGMTRARERLCLTNARRRRIFGQDQVNMPSRFIADIPRNLLDLEDEWQQ
PALGSVAPDTAGRGREPARHNLASILETEIEPDFADVEIVPVEPEESGVWLGMKVRHGKFGVGTIRKIEGQGDEQKVIVW
FNSVGPKKLLVRFAGLERV

Sequences:

>Translated_739_residues
MKLLDNLNPPQRAAVLHGEGPLLVLAGAGSGKTRVIVHRIAHLIRERGVPARQILAVTFTNKAAGEMRERVEKLVGGEVP
LIATFHSTCARILRREIHHLGYDSSFAIYDDKDSGKLLKEVMAGLGLDEKRFPATSLASAIDDCKNRGLAPEDLPADTFT
GEVVGRVYAAYQERLKKCNALDFGDLIMLTVRLFEEHPVVLERYRDQWRWILVDEYQDTNPIQYRLVRLLAGEHRNLCVV
GDDDQSIYRWRGADIRNILDFEKDFPGVTTIKLEQNYRSTRNILAAAGAVVARNRGRKAKTLWSENPPGDPIVYRRLADE
RDEARFVCREIERYVRRGGDLRDVAVFYRTNAQSRVVEDALVADGIPYHMVGGMRFYERMEVKDVLAYLRVLANPADEVS
LKRIINVPARGIGPGTVDKISELAVRRGITVLDALGEAATGGLLSSAPRGKVAAFAAVMERFFALRDQLPLAELTTRIIE
ESGYADRLRQERTDEAADRLANLQELVAAMEEFERTSDERGLAPFLEQVALVSDLERSGTGRESATLMTLHAAKGLEFPM
VFMIGMEEKLFPHVRSLDDPEAMEEERRLCYVGMTRARERLCLTNARRRRIFGQDQVNMPSRFIADIPRNLLDLEDEWQQ
PALGSVAPDTAGRGREPARHNLASILETEIEPDFADVEIVPVEPEESGVWLGMKVRHGKFGVGTIRKIEGQGDEQKVIVW
FNSVGPKKLLVRFAGLERV
>Mature_739_residues
MKLLDNLNPPQRAAVLHGEGPLLVLAGAGSGKTRVIVHRIAHLIRERGVPARQILAVTFTNKAAGEMRERVEKLVGGEVP
LIATFHSTCARILRREIHHLGYDSSFAIYDDKDSGKLLKEVMAGLGLDEKRFPATSLASAIDDCKNRGLAPEDLPADTFT
GEVVGRVYAAYQERLKKCNALDFGDLIMLTVRLFEEHPVVLERYRDQWRWILVDEYQDTNPIQYRLVRLLAGEHRNLCVV
GDDDQSIYRWRGADIRNILDFEKDFPGVTTIKLEQNYRSTRNILAAAGAVVARNRGRKAKTLWSENPPGDPIVYRRLADE
RDEARFVCREIERYVRRGGDLRDVAVFYRTNAQSRVVEDALVADGIPYHMVGGMRFYERMEVKDVLAYLRVLANPADEVS
LKRIINVPARGIGPGTVDKISELAVRRGITVLDALGEAATGGLLSSAPRGKVAAFAAVMERFFALRDQLPLAELTTRIIE
ESGYADRLRQERTDEAADRLANLQELVAAMEEFERTSDERGLAPFLEQVALVSDLERSGTGRESATLMTLHAAKGLEFPM
VFMIGMEEKLFPHVRSLDDPEAMEEERRLCYVGMTRARERLCLTNARRRRIFGQDQVNMPSRFIADIPRNLLDLEDEWQQ
PALGSVAPDTAGRGREPARHNLASILETEIEPDFADVEIVPVEPEESGVWLGMKVRHGKFGVGTIRKIEGQGDEQKVIVW
FNSVGPKKLLVRFAGLERV

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=752, Percent_Identity=41.3563829787234, Blast_Score=540, Evalue=1e-154,
Organism=Escherichia coli, GI48994965, Length=645, Percent_Identity=41.7054263565892, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1787196, Length=336, Percent_Identity=30.6547619047619, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322369, Length=736, Percent_Identity=28.6684782608696, Blast_Score=241, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6324477, Length=680, Percent_Identity=21.3235294117647, Blast_Score=122, Evalue=2e-28,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 83024; Mature: 83024

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLDNLNPPQRAAVLHGEGPLLVLAGAGSGKTRVIVHRIAHLIRERGVPARQILAVTFT
CCCCCCCCCCHHEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHEEEEEEC
NKAAGEMRERVEKLVGGEVPLIATFHSTCARILRREIHHLGYDSSFAIYDDKDSGKLLKE
CHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHH
VMAGLGLDEKRFPATSLASAIDDCKNRGLAPEDLPADTFTGEVVGRVYAAYQERLKKCNA
HHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
LDFGDLIMLTVRLFEEHPVVLERYRDQWRWILVDEYQDTNPIQYRLVRLLAGEHRNLCVV
CCCHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEE
GDDDQSIYRWRGADIRNILDFEKDFPGVTTIKLEQNYRSTRNILAAAGAVVARNRGRKAK
CCCCCHHHEECCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCHH
TLWSENPPGDPIVYRRLADERDEARFVCREIERYVRRGGDLRDVAVFYRTNAQSRVVEDA
HCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCHHHHHHHH
LVADGIPYHMVGGMRFYERMEVKDVLAYLRVLANPADEVSLKRIINVPARGIGPGTVDKI
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHH
SELAVRRGITVLDALGEAATGGLLSSAPRGKVAAFAAVMERFFALRDQLPLAELTTRIIE
HHHHHHCCCHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ESGYADRLRQERTDEAADRLANLQELVAAMEEFERTSDERGLAPFLEQVALVSDLERSGT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
GRESATLMTLHAAKGLEFPMVFMIGMEEKLFPHVRSLDDPEAMEEERRLCYVGMTRARER
CCCCCEEEEEEHHCCCCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHEEEEEHHHHHHH
LCLTNARRRRIFGQDQVNMPSRFIADIPRNLLDLEDEWQQPALGSVAPDTAGRGREPARH
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCHHHH
NLASILETEIEPDFADVEIVPVEPEESGVWLGMKVRHGKFGVGTIRKIEGQGDEQKVIVW
HHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCHHEEECCCCCCCEEEEE
FNSVGPKKLLVRFAGLERV
ECCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MKLLDNLNPPQRAAVLHGEGPLLVLAGAGSGKTRVIVHRIAHLIRERGVPARQILAVTFT
CCCCCCCCCCHHEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCHHHEEEEEEC
NKAAGEMRERVEKLVGGEVPLIATFHSTCARILRREIHHLGYDSSFAIYDDKDSGKLLKE
CHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHH
VMAGLGLDEKRFPATSLASAIDDCKNRGLAPEDLPADTFTGEVVGRVYAAYQERLKKCNA
HHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
LDFGDLIMLTVRLFEEHPVVLERYRDQWRWILVDEYQDTNPIQYRLVRLLAGEHRNLCVV
CCCHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCEEEE
GDDDQSIYRWRGADIRNILDFEKDFPGVTTIKLEQNYRSTRNILAAAGAVVARNRGRKAK
CCCCCHHHEECCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCHH
TLWSENPPGDPIVYRRLADERDEARFVCREIERYVRRGGDLRDVAVFYRTNAQSRVVEDA
HCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCHHHHHHHH
LVADGIPYHMVGGMRFYERMEVKDVLAYLRVLANPADEVSLKRIINVPARGIGPGTVDKI
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHH
SELAVRRGITVLDALGEAATGGLLSSAPRGKVAAFAAVMERFFALRDQLPLAELTTRIIE
HHHHHHCCCHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ESGYADRLRQERTDEAADRLANLQELVAAMEEFERTSDERGLAPFLEQVALVSDLERSGT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCC
GRESATLMTLHAAKGLEFPMVFMIGMEEKLFPHVRSLDDPEAMEEERRLCYVGMTRARER
CCCCCEEEEEEHHCCCCCCEEEEECCCHHHHHHHHCCCCHHHHHHHHHEEEEEHHHHHHH
LCLTNARRRRIFGQDQVNMPSRFIADIPRNLLDLEDEWQQPALGSVAPDTAGRGREPARH
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCHHHH
NLASILETEIEPDFADVEIVPVEPEESGVWLGMKVRHGKFGVGTIRKIEGQGDEQKVIVW
HHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCHHEEECCCCCCCEEEEE
FNSVGPKKLLVRFAGLERV
ECCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA