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The map label for this gene is cls [H]

Identifier: 39998461

GI number: 39998461

Start: 3705606

End: 3707048

Strand: Reverse

Name: cls [H]

Synonym: GSU3372

Alternate gene names: 39998461

Gene position: 3707048-3705606 (Counterclockwise)

Preceding gene: 39998466

Following gene: 39998459

Centisome position: 97.19

GC content: 61.33

Gene sequence:

>1443_bases
GTGATTTCATACTTGGATGACGTATTTTTATTTGTGTGCGCAGGAATCGCGATTGCCCTTTCGGTGGGCTCTGCCGGTCA
TGCCCTTGTCATGAAGCGAGACCCCCGGTCAGCACTCGGGTGGATCGTTGTCTGCCTCACGGTCCCGCTGTTCGGTCCCA
TTTTCTACTGGAGCATGGGGGTAAACCGCATCTTCCGCCGGGCCCGCAAGTGGCAGGAAACAGGGCGCCGTCTGGCTGGG
GGAGAGCACTTCCGCTCAGCCCAGGAGCATGAAAGTGCGCCGACTCCTCCGGCGGGGGCCGAATACCTCTCCGAGCTGCG
CCATCTGGCCGATCGGGTGGTGGGAGCCCCTCTGGTCCAGGGAAACCGTATAATCCCCCTGGATGGTGGCGAAGGGGCGT
ACCCCGCCATGCTGGAAGCCATCGCCCATGCCCGATCCTCGGTCCACCTTTCTACCTATATCTTCGATTCCGACAGCACC
GGCCGCCGGTTCATCACTACGCTGGCCGCCGCCGCGGCGCGTGGTGTCATCGTGCGGGTGATCGTCGACGGCGTCGGTGA
AAAGTATTCATGGCCCCGTGCCAGGACTCTCATGGAGGGGACGGGAATCAGGGTTGAGCGGTTCCTGCCGTTGCGACAGG
GGATCTACATGAATCTGCGCAACCACCGCAAAGTCTTGGTGGTGGACGGCCAGCTGGCATTTACGGGGGGGATGAATATC
GGGGACCGGCATGTGGCTGAAAAGGCGGGTGACGCCGTGTCGCGGGATGTTCACTTCTCGGTGGCGGGTCCGGTGGTGGC
GGGGCTTCAGCAGGTCTTCCTTGAGGATTGGCACTTCGTCACCGGCGAACTTCTTGATGACACCCGGTATTTCCCTCCGA
TCCAGCCGGCCGGTCATGCCCTGGCCCGTCCCATCGGCGACGGGCCCGACAAGGAATACCATACGCTCCATTGGATCATC
ATGGGAGCCCTGTCGTGCGCCACTCGGACGGTTCAGATCATGACACCGTACTTCATCCCCGACCGCTCACTTGTTTCGGC
CCTGGTCACTACGGCGTTGCGGGGGGTGGAGGTCACCCTCGTTTTGCCTGAGAAGAACAATCTTCCCTTTGTCCACTGGG
CCACCAGGGCCTATCTCTGGGAGCTTATCCAGCAGGGAATCAGAGTGTTTTACCAGCCGCCCCCTTTTGCCCACACCAAG
CTTTTCATCGTCGACGGCCTCTGGAGCATGATCGGCTCGGCCAACCTGGATCCCCGCAGCCTTCGGCTCAACTTCGAGCT
CAATCTGGAGGTTTACGATCGGGAGTTGGCCGGAACCCTTTCCCGTCACTTCGCCGTAACCGTTGCCCGCTCGCGACAGG
TGAGCCTGACCGAGATGGACGGCCGCCCACTCCCTGAAAAAGTGCGGGACGCCACGGCCAAGCTCTTTTCTCCCTATCTG
TAG

Upstream 100 bases:

>100_bases
AGTAAAGGGTCGGCAGGCATGCCGCAATGGGGCGGCTGTACACTGCCAGGCCGGAGCCCGCGTGCGACGAGGTTTTCGCA
CCGCCGCCGAGGGATGGGGC

Downstream 100 bases:

>100_bases
TCCTGGCTACAACGGCTCGCGGTATCGGGTCGCTGTCATTTCCGGTAGCGCTCGATGGCAACGAGTGCCCTGTCCAGAGC
TGCCGGGCAGTGGGCCTCGA

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MISYLDDVFLFVCAGIAIALSVGSAGHALVMKRDPRSALGWIVVCLTVPLFGPIFYWSMGVNRIFRRARKWQETGRRLAG
GEHFRSAQEHESAPTPPAGAEYLSELRHLADRVVGAPLVQGNRIIPLDGGEGAYPAMLEAIAHARSSVHLSTYIFDSDST
GRRFITTLAAAAARGVIVRVIVDGVGEKYSWPRARTLMEGTGIRVERFLPLRQGIYMNLRNHRKVLVVDGQLAFTGGMNI
GDRHVAEKAGDAVSRDVHFSVAGPVVAGLQQVFLEDWHFVTGELLDDTRYFPPIQPAGHALARPIGDGPDKEYHTLHWII
MGALSCATRTVQIMTPYFIPDRSLVSALVTTALRGVEVTLVLPEKNNLPFVHWATRAYLWELIQQGIRVFYQPPPFAHTK
LFIVDGLWSMIGSANLDPRSLRLNFELNLEVYDRELAGTLSRHFAVTVARSRQVSLTEMDGRPLPEKVRDATAKLFSPYL

Sequences:

>Translated_480_residues
MISYLDDVFLFVCAGIAIALSVGSAGHALVMKRDPRSALGWIVVCLTVPLFGPIFYWSMGVNRIFRRARKWQETGRRLAG
GEHFRSAQEHESAPTPPAGAEYLSELRHLADRVVGAPLVQGNRIIPLDGGEGAYPAMLEAIAHARSSVHLSTYIFDSDST
GRRFITTLAAAAARGVIVRVIVDGVGEKYSWPRARTLMEGTGIRVERFLPLRQGIYMNLRNHRKVLVVDGQLAFTGGMNI
GDRHVAEKAGDAVSRDVHFSVAGPVVAGLQQVFLEDWHFVTGELLDDTRYFPPIQPAGHALARPIGDGPDKEYHTLHWII
MGALSCATRTVQIMTPYFIPDRSLVSALVTTALRGVEVTLVLPEKNNLPFVHWATRAYLWELIQQGIRVFYQPPPFAHTK
LFIVDGLWSMIGSANLDPRSLRLNFELNLEVYDRELAGTLSRHFAVTVARSRQVSLTEMDGRPLPEKVRDATAKLFSPYL
>Mature_480_residues
MISYLDDVFLFVCAGIAIALSVGSAGHALVMKRDPRSALGWIVVCLTVPLFGPIFYWSMGVNRIFRRARKWQETGRRLAG
GEHFRSAQEHESAPTPPAGAEYLSELRHLADRVVGAPLVQGNRIIPLDGGEGAYPAMLEAIAHARSSVHLSTYIFDSDST
GRRFITTLAAAAARGVIVRVIVDGVGEKYSWPRARTLMEGTGIRVERFLPLRQGIYMNLRNHRKVLVVDGQLAFTGGMNI
GDRHVAEKAGDAVSRDVHFSVAGPVVAGLQQVFLEDWHFVTGELLDDTRYFPPIQPAGHALARPIGDGPDKEYHTLHWII
MGALSCATRTVQIMTPYFIPDRSLVSALVTTALRGVEVTLVLPEKNNLPFVHWATRAYLWELIQQGIRVFYQPPPFAHTK
LFIVDGLWSMIGSANLDPRSLRLNFELNLEVYDRELAGTLSRHFAVTVARSRQVSLTEMDGRPLPEKVRDATAKLFSPYL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=484, Percent_Identity=30.3719008264463, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1787007, Length=353, Percent_Identity=30.5949008498584, Blast_Score=138, Evalue=9e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 53269; Mature: 53269

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISYLDDVFLFVCAGIAIALSVGSAGHALVMKRDPRSALGWIVVCLTVPLFGPIFYWSMG
CCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
VNRIFRRARKWQETGRRLAGGEHFRSAQEHESAPTPPAGAEYLSELRHLADRVVGAPLVQ
HHHHHHHHHHHHHHHHHCCCCHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEC
GNRIIPLDGGEGAYPAMLEAIAHARSSVHLSTYIFDSDSTGRRFITTLAAAAARGVIVRV
CCEEEEECCCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHHCCEEEEE
IVDGVGEKYSWPRARTLMEGTGIRVERFLPLRQGIYMNLRNHRKVLVVDGQLAFTGGMNI
EECCCCCCCCCCHHHHHHCCCCCCCEECCHHHHCCEEEECCCCEEEEECCEEEEECCCCC
GDRHVAEKAGDAVSRDVHFSVAGPVVAGLQQVFLEDWHFVTGELLDDTRYFPPIQPAGHA
CHHHHHHHHCCHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
LARPIGDGPDKEYHTLHWIIMGALSCATRTVQIMTPYFIPDRSLVSALVTTALRGVEVTL
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCEECCCHHHHHHHHHHHHCCEEEEE
VLPEKNNLPFVHWATRAYLWELIQQGIRVFYQPPPFAHTKLFIVDGLWSMIGSANLDPRS
EEECCCCCCEEEHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEEHHHHHHHCCCCCCCCE
LRLNFELNLEVYDRELAGTLSRHFAVTVARSRQVSLTEMDGRPLPEKVRDATAKLFSPYL
EEEEEEEEEEEECHHHHHHHHHHEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MISYLDDVFLFVCAGIAIALSVGSAGHALVMKRDPRSALGWIVVCLTVPLFGPIFYWSMG
CCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
VNRIFRRARKWQETGRRLAGGEHFRSAQEHESAPTPPAGAEYLSELRHLADRVVGAPLVQ
HHHHHHHHHHHHHHHHHCCCCHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCEEC
GNRIIPLDGGEGAYPAMLEAIAHARSSVHLSTYIFDSDSTGRRFITTLAAAAARGVIVRV
CCEEEEECCCCCCHHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHHCCEEEEE
IVDGVGEKYSWPRARTLMEGTGIRVERFLPLRQGIYMNLRNHRKVLVVDGQLAFTGGMNI
EECCCCCCCCCCHHHHHHCCCCCCCEECCHHHHCCEEEECCCCEEEEECCEEEEECCCCC
GDRHVAEKAGDAVSRDVHFSVAGPVVAGLQQVFLEDWHFVTGELLDDTRYFPPIQPAGHA
CHHHHHHHHCCHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
LARPIGDGPDKEYHTLHWIIMGALSCATRTVQIMTPYFIPDRSLVSALVTTALRGVEVTL
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEECCEECCCHHHHHHHHHHHHCCEEEEE
VLPEKNNLPFVHWATRAYLWELIQQGIRVFYQPPPFAHTKLFIVDGLWSMIGSANLDPRS
EEECCCCCCEEEHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEEHHHHHHHCCCCCCCCE
LRLNFELNLEVYDRELAGTLSRHFAVTVARSRQVSLTEMDGRPLPEKVRDATAKLFSPYL
EEEEEEEEEEEECHHHHHHHHHHEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA