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The map label for this gene is pyk [H]

Identifier: 39998420

GI number: 39998420

Start: 3660079

End: 3661521

Strand: Reverse

Name: pyk [H]

Synonym: GSU3331

Alternate gene names: 39998420

Gene position: 3661521-3660079 (Counterclockwise)

Preceding gene: 39998421

Following gene: 39998419

Centisome position: 96.0

GC content: 64.38

Gene sequence:

>1443_bases
ATGGATACACTGTCACGCAAAACCAAGATCATCGCCACTCTGGGGCCCGTCAGCTCCTCGCCCGACATGATCCGGCAGCT
GATGGACGCAGGAGTCGATGTCTTCCGGCTCAACTTTTCCCACGGCTCCAACGACCAGCGGCGGGAGGTAATCGCCGCCA
TCCGGCGTCTTTCCGCCGAACGGGGCAAGGAGATAGGCATTCTGGCCGATCTCCAGGGGCCCAAGATCAGGACCGGCAGG
ATGGAGAACGGTGCCATTCCGCTGGTGCGGGGCGACTCGCTCGACATTACCACCGATGAGGTCCTGGGGCGCCCCGGTCT
CATTTCCACCATCTACCAGTCTCTCCCCCACGACGTGAAGCCCGGCTCACGCATCCTGCTGGACGACGGCCTTATCGAGT
TGCGGGTCCAGTCCGTGTCCGGCGCCACGGTCCGTTGCACCGTGGTCCAGGGGGGCATGCTCAAGGATCTGAAAGGGATC
AACCTCCCCGGCGTCAAGGTTTCTGCGCCGTCGCTGTCGGAGAAGGACCTCCGCGATCTGGATTTCTGCCTGGAGGTGGG
GGTCGACTACATTGCCCTGTCCTTTGTCCGCACTGCCGCCGACGTGGAGGGGCTGAAGCGGATCCTCTTCGAACGGGACG
TCCAGGTGCCGGTGGTGGCCAAGATCGAGAAGCCCGAGGCCCTGCGCAACTTCAAAAGCATCCTCAAGGTTGCCGATGCG
GTCATGGTGGCCCGGGGCGATCTGGGGGTGGAAATCAGCCCGGAAAAGGTCCCCCTGTTCCAGAAAAAGATCATCCGGGC
CTGCAACGAGGCCGGCAAACCGGTGATCACCGCCACCCAGATGCTTGAATCCATGATTAGCCATCCGCGCCCCACCCGGG
CCGAGACCTCGGACGTGGCCAACGCCATCCTCGACGGAACCGATGCGGTCATGCTCTCGGGTGAGACCGCCTCGGGGCAG
TTTCCCCTGGAAGCGGTGCGTACCATGGACAAGGTGGCCCTCGACGTTGAGCGCTTCGCTCAGGTGGAGGACGGCTCGGG
GCCACGCCGCCACAGCGTCAGCATCGCCGAGGCGGTGGCCGAGGCCGCCTGCCACGCTGCCGTCATTCTGAAGGCGAAGG
CCGTGGCCTGCATGACCCAGTCGGGGAGCACGGCTGCCCGCATCTCACGCTACCGGCCTCCGCTGCCGATCCTCGCCTTC
ACCGGTTCGGTGGACACCATGCGACGGCTGTCGCTCTACTGGGGAGTCAAGGCATACCCCATCGGCACCATGGCCGGCAC
CGACGAGCAGATAGTGGCCGTGGAGTCGACGCTCCTGTCCGGTGGCTACCGCAAGGGCGACGTGGTGGTCATCACCATGG
GTGTGCCGGTGGAGGCCCGGGGGTCCACCAACCTCATGAAGGTCCACAAGCTGGGAACCGGCCAGTTCTTCGAGATATTT
TGA

Upstream 100 bases:

>100_bases
AGGTGGACGAACTTCCCTGCTTCGAGTGCCACAAGTCTCCGAGTCCGGCCCATAAGGGACGGGTCGGCAAATTCCACCAC
TGACGCCTATGCCGAAGCCC

Downstream 100 bases:

>100_bases
GCCCGTCCCTGCGATTTGCCGCCGGTTGAAGGAGGAACGCTGATGCGCAAGGTGTTCGACGAGACCAAGATCAACCACAT
GGTGCTTGCCAACCGCCTCG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MDTLSRKTKIIATLGPVSSSPDMIRQLMDAGVDVFRLNFSHGSNDQRREVIAAIRRLSAERGKEIGILADLQGPKIRTGR
MENGAIPLVRGDSLDITTDEVLGRPGLISTIYQSLPHDVKPGSRILLDDGLIELRVQSVSGATVRCTVVQGGMLKDLKGI
NLPGVKVSAPSLSEKDLRDLDFCLEVGVDYIALSFVRTAADVEGLKRILFERDVQVPVVAKIEKPEALRNFKSILKVADA
VMVARGDLGVEISPEKVPLFQKKIIRACNEAGKPVITATQMLESMISHPRPTRAETSDVANAILDGTDAVMLSGETASGQ
FPLEAVRTMDKVALDVERFAQVEDGSGPRRHSVSIAEAVAEAACHAAVILKAKAVACMTQSGSTAARISRYRPPLPILAF
TGSVDTMRRLSLYWGVKAYPIGTMAGTDEQIVAVESTLLSGGYRKGDVVVITMGVPVEARGSTNLMKVHKLGTGQFFEIF

Sequences:

>Translated_480_residues
MDTLSRKTKIIATLGPVSSSPDMIRQLMDAGVDVFRLNFSHGSNDQRREVIAAIRRLSAERGKEIGILADLQGPKIRTGR
MENGAIPLVRGDSLDITTDEVLGRPGLISTIYQSLPHDVKPGSRILLDDGLIELRVQSVSGATVRCTVVQGGMLKDLKGI
NLPGVKVSAPSLSEKDLRDLDFCLEVGVDYIALSFVRTAADVEGLKRILFERDVQVPVVAKIEKPEALRNFKSILKVADA
VMVARGDLGVEISPEKVPLFQKKIIRACNEAGKPVITATQMLESMISHPRPTRAETSDVANAILDGTDAVMLSGETASGQ
FPLEAVRTMDKVALDVERFAQVEDGSGPRRHSVSIAEAVAEAACHAAVILKAKAVACMTQSGSTAARISRYRPPLPILAF
TGSVDTMRRLSLYWGVKAYPIGTMAGTDEQIVAVESTLLSGGYRKGDVVVITMGVPVEARGSTNLMKVHKLGTGQFFEIF
>Mature_480_residues
MDTLSRKTKIIATLGPVSSSPDMIRQLMDAGVDVFRLNFSHGSNDQRREVIAAIRRLSAERGKEIGILADLQGPKIRTGR
MENGAIPLVRGDSLDITTDEVLGRPGLISTIYQSLPHDVKPGSRILLDDGLIELRVQSVSGATVRCTVVQGGMLKDLKGI
NLPGVKVSAPSLSEKDLRDLDFCLEVGVDYIALSFVRTAADVEGLKRILFERDVQVPVVAKIEKPEALRNFKSILKVADA
VMVARGDLGVEISPEKVPLFQKKIIRACNEAGKPVITATQMLESMISHPRPTRAETSDVANAILDGTDAVMLSGETASGQ
FPLEAVRTMDKVALDVERFAQVEDGSGPRRHSVSIAEAVAEAACHAAVILKAKAVACMTQSGSTAARISRYRPPLPILAF
TGSVDTMRRLSLYWGVKAYPIGTMAGTDEQIVAVESTLLSGGYRKGDVVVITMGVPVEARGSTNLMKVHKLGTGQFFEIF

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=490, Percent_Identity=39.5918367346939, Blast_Score=328, Evalue=1e-89,
Organism=Homo sapiens, GI33286420, Length=490, Percent_Identity=39.5918367346939, Blast_Score=328, Evalue=1e-89,
Organism=Homo sapiens, GI10835121, Length=487, Percent_Identity=41.2731006160164, Blast_Score=327, Evalue=2e-89,
Organism=Homo sapiens, GI32967597, Length=487, Percent_Identity=41.2731006160164, Blast_Score=326, Evalue=2e-89,
Organism=Homo sapiens, GI33286418, Length=487, Percent_Identity=39.4250513347023, Blast_Score=318, Evalue=5e-87,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=38.8888888888889, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=38.8888888888889, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=34.1772151898734, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=34.1772151898734, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=33.4883720930233, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1787965, Length=472, Percent_Identity=42.7966101694915, Blast_Score=347, Evalue=1e-96,
Organism=Escherichia coli, GI1788160, Length=477, Percent_Identity=40.041928721174, Blast_Score=305, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI17544584, Length=483, Percent_Identity=40.5797101449275, Blast_Score=336, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17506829, Length=487, Percent_Identity=41.0677618069815, Blast_Score=309, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI71984413, Length=487, Percent_Identity=41.0677618069815, Blast_Score=309, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=41.0677618069815, Blast_Score=309, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI17506831, Length=487, Percent_Identity=41.0677618069815, Blast_Score=308, Evalue=4e-84,
Organism=Saccharomyces cerevisiae, GI6319279, Length=481, Percent_Identity=39.0852390852391, Blast_Score=291, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6324923, Length=424, Percent_Identity=40.5660377358491, Blast_Score=279, Evalue=6e-76,
Organism=Drosophila melanogaster, GI24648964, Length=430, Percent_Identity=43.2558139534884, Blast_Score=324, Evalue=7e-89,
Organism=Drosophila melanogaster, GI28571814, Length=430, Percent_Identity=43.2558139534884, Blast_Score=324, Evalue=9e-89,
Organism=Drosophila melanogaster, GI24648966, Length=406, Percent_Identity=42.3645320197044, Blast_Score=292, Evalue=4e-79,
Organism=Drosophila melanogaster, GI24581235, Length=424, Percent_Identity=36.3207547169811, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24646914, Length=275, Percent_Identity=39.2727272727273, Blast_Score=182, Evalue=6e-46,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51684; Mature: 51684

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLSRKTKIIATLGPVSSSPDMIRQLMDAGVDVFRLNFSHGSNDQRREVIAAIRRLSAE
CCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
RGKEIGILADLQGPKIRTGRMENGAIPLVRGDSLDITTDEVLGRPGLISTIYQSLPHDVK
CCCCEEEEEECCCCCEECCCCCCCCEEEEECCCCCCCHHHHCCCCCHHHHHHHHCCCCCC
PGSRILLDDGLIELRVQSVSGATVRCTVVQGGMLKDLKGINLPGVKVSAPSLSEKDLRDL
CCCEEEEECCEEEEEEEECCCCEEEEEEECCCCHHHHCCCCCCCCEEECCCCCHHHHHHH
DFCLEVGVDYIALSFVRTAADVEGLKRILFERDVQVPVVAKIEKPEALRNFKSILKVADA
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHH
VMVARGDLGVEISPEKVPLFQKKIIRACNEAGKPVITATQMLESMISHPRPTRAETSDVA
HHEECCCCCCEECCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCHHHHH
NAILDGTDAVMLSGETASGQFPLEAVRTMDKVALDVERFAQVEDGSGPRRHSVSIAEAVA
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
EAACHAAVILKAKAVACMTQSGSTAARISRYRPPLPILAFTGSVDTMRRLSLYWGVKAYP
HHHHHHHHHHHHHHHEEEECCCCHHHHHHHCCCCCCEEEECCCHHHHHHHHHHCCCEEEE
IGTMAGTDEQIVAVESTLLSGGYRKGDVVVITMGVPVEARGSTNLMKVHKLGTGQFFEIF
CCCCCCCCCEEEEEHHHHHCCCCCCCCEEEEEECCCEECCCCCCEEEEEECCCCCEEEEC
>Mature Secondary Structure
MDTLSRKTKIIATLGPVSSSPDMIRQLMDAGVDVFRLNFSHGSNDQRREVIAAIRRLSAE
CCCCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
RGKEIGILADLQGPKIRTGRMENGAIPLVRGDSLDITTDEVLGRPGLISTIYQSLPHDVK
CCCCEEEEEECCCCCEECCCCCCCCEEEEECCCCCCCHHHHCCCCCHHHHHHHHCCCCCC
PGSRILLDDGLIELRVQSVSGATVRCTVVQGGMLKDLKGINLPGVKVSAPSLSEKDLRDL
CCCEEEEECCEEEEEEEECCCCEEEEEEECCCCHHHHCCCCCCCCEEECCCCCHHHHHHH
DFCLEVGVDYIALSFVRTAADVEGLKRILFERDVQVPVVAKIEKPEALRNFKSILKVADA
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHH
VMVARGDLGVEISPEKVPLFQKKIIRACNEAGKPVITATQMLESMISHPRPTRAETSDVA
HHEECCCCCCEECCCCCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCCCHHHHH
NAILDGTDAVMLSGETASGQFPLEAVRTMDKVALDVERFAQVEDGSGPRRHSVSIAEAVA
HHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
EAACHAAVILKAKAVACMTQSGSTAARISRYRPPLPILAFTGSVDTMRRLSLYWGVKAYP
HHHHHHHHHHHHHHHEEEECCCCHHHHHHHCCCCCCEEEECCCHHHHHHHHHHCCCEEEE
IGTMAGTDEQIVAVESTLLSGGYRKGDVVVITMGVPVEARGSTNLMKVHKLGTGQFFEIF
CCCCCCCCCEEEEEHHHHHCCCCCCCCEEEEEECCCEECCCCCCEEEEEECCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8436141; 1447787 [H]