Click here to switch to the map view.

The map label for this gene is fadH [C]

Identifier: 39998419

GI number: 39998419

Start: 3658912

End: 3660036

Strand: Reverse

Name: fadH [C]

Synonym: GSU3330

Alternate gene names: 39998419

Gene position: 3660036-3658912 (Counterclockwise)

Preceding gene: 39998420

Following gene: 39998418

Centisome position: 95.96

GC content: 65.78

Gene sequence:

>1125_bases
ATGCGCAAGGTGTTCGACGAGACCAAGATCAACCACATGGTGCTTGCCAACCGCCTCGTCCGCTCCGCCACCTGGGAGGG
AATGTGCGGCGAGGACGGCTGTCCCTCGGAAAAGCTGGCGGGTTACTATGCCACCCTCGCCCGGGGCGGGGTGGGGCTCA
TCATCACCGGCTACGCCTTCGTTCGCCCTGACGGGAAACAACTTCCCGGGCAGATGGGTGTCCATGAGGGCTCCTTCGCC
GCCGCCGCGCGGCAGGTAACCGGCGCCGTCCACCGGGAAGGGGGGAAAATCTGCCTCCAACTGGTTCACTGCGGGGGACA
GGCGCCGTCCCGGGCCGCCGGCTGCCAGCCGGTGGCTCCCTCGGCAATCAAGGCGGTGCAGTATCCGGAACTGCCGCGGG
AACTACCTATGGCCGAAGTGGTTGAGCTGGCGGGCCTGTTCGCCGACGGCGCCCGCCGCGCACGCGAGTGGGGCTTCGAC
GCGGTCCAGCTCCACGCCGCCCACGGCTACCTGATCAACCAGTTCCTTTCTCCTCTTACCAACCATCGGACCGATCACTA
CGGTGGCAACCTGGAAAGTCGAGCCCTGTTCCTTCTTGAGACCTGCCGTGCCGTGCGCCGGGCGGTGGGAAAAGAGTTCC
CCGTCATGGTCAAGCTCAACGGCTCCGACAACCTGGCCGGCGGATTCGACCTGGACGAGGCGGTTCAAGTTGCCCGGATG
CTCGATGAAGAAGGCATCGACGCCATCGAGGTGTCGGGCGGCACCCCCGCCTCGGGAGCCCGAACCGCAGTGCGTCAGGG
GATCGAGACCCGGGAGCAGGAAGCCTACAACCTTCCGTCCGCCTATCGGATCAAGAATGCCGTCTCTTGCCCTGTCATGG
TGGTGGGGGGATTCCGTTCCTTCGAGCTGGTGGAAGGGGTCATCCGCCGGGAAGAGGCCGACTACGTGGCCCTTTCCCGG
CCCCTCATCCGCGAACCGCACCTTCCCCTCCGCTGGCAGCAGGGCGACGAGAGCCGGGCCCGCTGTATCTCATGCAACGG
CTGTTTCAAGCCGGGGCTCAAGGAGGGGGGGATCTGCTGCGTGGTGGACAAGATGGAGCGGGAGAGCCGGGAGTTTTCTC
TGTAA

Upstream 100 bases:

>100_bases
CACCAACCTCATGAAGGTCCACAAGCTGGGAACCGGCCAGTTCTTCGAGATATTTTGAGCCCGTCCCTGCGATTTGCCGC
CGGTTGAAGGAGGAACGCTG

Downstream 100 bases:

>100_bases
CGGGAGGAATGCGACGTATTCCGTCATGGGTCCGGGAGAGGATTGCCCGGGCCTTGCCTTTTTTGAGGGGAACGATGGCG
GAACCAACTCTGGCTGAAAA

Product: Oye family NADH-dependent flavin oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MRKVFDETKINHMVLANRLVRSATWEGMCGEDGCPSEKLAGYYATLARGGVGLIITGYAFVRPDGKQLPGQMGVHEGSFA
AAARQVTGAVHREGGKICLQLVHCGGQAPSRAAGCQPVAPSAIKAVQYPELPRELPMAEVVELAGLFADGARRAREWGFD
AVQLHAAHGYLINQFLSPLTNHRTDHYGGNLESRALFLLETCRAVRRAVGKEFPVMVKLNGSDNLAGGFDLDEAVQVARM
LDEEGIDAIEVSGGTPASGARTAVRQGIETREQEAYNLPSAYRIKNAVSCPVMVVGGFRSFELVEGVIRREEADYVALSR
PLIREPHLPLRWQQGDESRARCISCNGCFKPGLKEGGICCVVDKMERESREFSL

Sequences:

>Translated_374_residues
MRKVFDETKINHMVLANRLVRSATWEGMCGEDGCPSEKLAGYYATLARGGVGLIITGYAFVRPDGKQLPGQMGVHEGSFA
AAARQVTGAVHREGGKICLQLVHCGGQAPSRAAGCQPVAPSAIKAVQYPELPRELPMAEVVELAGLFADGARRAREWGFD
AVQLHAAHGYLINQFLSPLTNHRTDHYGGNLESRALFLLETCRAVRRAVGKEFPVMVKLNGSDNLAGGFDLDEAVQVARM
LDEEGIDAIEVSGGTPASGARTAVRQGIETREQEAYNLPSAYRIKNAVSCPVMVVGGFRSFELVEGVIRREEADYVALSR
PLIREPHLPLRWQQGDESRARCISCNGCFKPGLKEGGICCVVDKMERESREFSL
>Mature_374_residues
MRKVFDETKINHMVLANRLVRSATWEGMCGEDGCPSEKLAGYYATLARGGVGLIITGYAFVRPDGKQLPGQMGVHEGSFA
AAARQVTGAVHREGGKICLQLVHCGGQAPSRAAGCQPVAPSAIKAVQYPELPRELPMAEVVELAGLFADGARRAREWGFD
AVQLHAAHGYLINQFLSPLTNHRTDHYGGNLESRALFLLETCRAVRRAVGKEFPVMVKLNGSDNLAGGFDLDEAVQVARM
LDEEGIDAIEVSGGTPASGARTAVRQGIETREQEAYNLPSAYRIKNAVSCPVMVVGGFRSFELVEGVIRREEADYVALSR
PLIREPHLPLRWQQGDESRARCISCNGCFKPGLKEGGICCVVDKMERESREFSL

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=361, Percent_Identity=32.1329639889197, Blast_Score=154, Evalue=7e-39,
Organism=Escherichia coli, GI1787939, Length=357, Percent_Identity=29.9719887955182, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17565138, Length=347, Percent_Identity=31.4121037463977, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI72001454, Length=323, Percent_Identity=31.5789473684211, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17559802, Length=345, Percent_Identity=28.9855072463768, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17540738, Length=347, Percent_Identity=29.1066282420749, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17559804, Length=356, Percent_Identity=28.6516853932584, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17564188, Length=351, Percent_Identity=28.2051282051282, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17566914, Length=256, Percent_Identity=30.859375, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17566916, Length=346, Percent_Identity=26.0115606936416, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321973, Length=217, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6325086, Length=224, Percent_Identity=28.125, Blast_Score=73, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 40656; Mature: 40656

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVFDETKINHMVLANRLVRSATWEGMCGEDGCPSEKLAGYYATLARGGVGLIITGYAF
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEE
VRPDGKQLPGQMGVHEGSFAAAARQVTGAVHREGGKICLQLVHCGGQAPSRAAGCQPVAP
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCH
SAIKAVQYPELPRELPMAEVVELAGLFADGARRAREWGFDAVQLHAAHGYLINQFLSPLT
HHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHH
NHRTDHYGGNLESRALFLLETCRAVRRAVGKEFPVMVKLNGSDNLAGGFDLDEAVQVARM
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHH
LDEEGIDAIEVSGGTPASGARTAVRQGIETREQEAYNLPSAYRIKNAVSCPVMVVGGFRS
HHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEECCCCH
FELVEGVIRREEADYVALSRPLIREPHLPLRWQQGDESRARCISCNGCFKPGLKEGGICC
HHHHHHHHHHCCCCCHHHCCCHHCCCCCCEEECCCCCCCCEEEECCCCCCCCCCCCCEEE
VVDKMERESREFSL
EEEHHHHHHHCCCC
>Mature Secondary Structure
MRKVFDETKINHMVLANRLVRSATWEGMCGEDGCPSEKLAGYYATLARGGVGLIITGYAF
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEEEE
VRPDGKQLPGQMGVHEGSFAAAARQVTGAVHREGGKICLQLVHCGGQAPSRAAGCQPVAP
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCH
SAIKAVQYPELPRELPMAEVVELAGLFADGARRAREWGFDAVQLHAAHGYLINQFLSPLT
HHHHHHCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHH
NHRTDHYGGNLESRALFLLETCRAVRRAVGKEFPVMVKLNGSDNLAGGFDLDEAVQVARM
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHH
LDEEGIDAIEVSGGTPASGARTAVRQGIETREQEAYNLPSAYRIKNAVSCPVMVVGGFRS
HHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEECCCCH
FELVEGVIRREEADYVALSRPLIREPHLPLRWQQGDESRARCISCNGCFKPGLKEGGICC
HHHHHHHHHHCCCCCHHHCCCHHCCCCCCEEECCCCCCCCEEEECCCCCCCCCCCCCEEE
VVDKMERESREFSL
EEEHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]