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The map label for this gene is hemD [H]

Identifier: 39998375

GI number: 39998375

Start: 3605124

End: 3606671

Strand: Direct

Name: hemD [H]

Synonym: GSU3286

Alternate gene names: 39998375

Gene position: 3605124-3606671 (Clockwise)

Preceding gene: 39998374

Following gene: 39998376

Centisome position: 94.52

GC content: 65.18

Gene sequence:

>1548_bases
ATGACCATGTCCACAGCTTCCCGCACCAATTGCCTTGTCTACCTCATCGGCGCCGGCCCCGGCGACCCGGGCCTCATTAC
GGTTCGGGGCCGGGAATGCATCGCACGGGCCGATGTGGTCATTTACGATTACCTGGCCAACGACGAACTCCTCTCCTTCG
CCCGTCCCGATGCGGAACTCATTTATGCCGGCAAGGTTGGGGGGCAGCATAACCGGGCCCAGTGGCAGATCAATGAGCTC
CTGGTCCGCAAGGCGCTTGAGGGACGCATCGTGGCGCGCCTCAAGGGTGGTGATCCCTTCGTCTTCGGCCGGGGCGGCGA
GGAGTGCGAGGCCCTGGTCACCGCCGGCGTACCCTTCGAGGTGGTGCCGGGAGTGACTGCGGGAATCGGGGCAGCGGCCT
ACGCCGGCATCCCCCTTACCCACCGCAACGTCACCACATCGGTTGCCTTTGTCACGGGTCACGAGAGCCCGGGCAAGAAA
TCATCGGACATCGATTGGGAGGGGCTCTCCCTCGGCAGCGGCACTGTTGTGTTTTACATGGGAGTCAAGAGTCTCCCGCA
CATTACGGAAAACCTCATGGCCCACGGCCGGCGCGCCGACACCCCGGTGGCCCTCGTCCGCTGGGGAACCCGACCGGAGC
AGGAAGTTCTGGTGGGAACGCTGGCCGACATCGCCGACAAGGTCCGCCGCACAGGGTTCAAGGCACCGGCCATAACCGTT
GTGGGCGAGGTGGTCCGGCTGAGGAAAACGCTTCGCTGGTTCGACAACCGGCCCCTTTTCGGTCGCAGTATCATGGTCAC
CCGCGCCGCCGACCAAGCGGGCGAATTCAGCGTCATGCTCCGTTCCCTGGGCGCACGGGTGCTTGAGTGTCCCACCATCG
CCATCGCGCCACCCGAGAGCTGGGATGACCTCGACAGCGCCATCAGCCGCATCGATACCTTTGACTGGGCCATCTTCACC
TCATTCAACGCGGTTCGCTTCTTCTTTCAGCGGCTGGACGCCGCGGGCCGTGACTCGCGCGCCCTGGGAGCCTGCCGGAT
CTGCGTCGTGGGCCCGAAAACGGCCGAAGCCCTTGCGCCCTACGGAATCCGGCCCGACCTGGTGGCCGCAGACTATAAAG
GGGAAGGGGTTATCGAGGCGCTTCGCGACGTACCGTTGGCCGGCAAGCGGGTCCTGTTTCCCAAAGGGGACCGGGCGCGG
GACGTGATCCCCACGGGCCTTGCAGCCTTCGGCGCCCATGTCGAGGCGCCGGTCGCCTACCGCAACGCTGCGCCCGACGC
CCTCCCGGCCGGCACTATCGAGGAACTGGAGGACCGGCGCATCCACTGCGTAACGTTTACCTCTTCTTCCACCGTGCAGA
ACCTGGCGGCCATTCTTGGGGAGAACAGAATGCTCCACCTGTTGGAAGGGGTTATTATAGCCTCCATCGGCCCCATCACC
TCCCGCAGTTGCCGCGAGCTGGGGCTGCAGGTCCACATCGAGCCGCAGGAGTACACCCTGGCGGCTCTCACCGCTGAGAT
GGTCCGCCACTTCTCGGCACCCGCCTGA

Upstream 100 bases:

>100_bases
CCGTCTCCGGCCACAGGGGCATGGAGGAATTCAGAGGAGGCCTGCCGGACCTTCTTCCTCTGAAATCCTCTGTGCCCCCT
GTGGTCGGCTTTTTTGCACA

Downstream 100 bases:

>100_bases
GCCACGTTCACGGGGCAGTAGAGCTGAATGGAGCCCTTGCTCACCGATACCCATTGCCATCTGGACGCCCCGCCCTTACG
AGGACGCCTCGACGAGGTCC

Product: uroporphyrinogen III synthase/methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MTMSTASRTNCLVYLIGAGPGDPGLITVRGRECIARADVVIYDYLANDELLSFARPDAELIYAGKVGGQHNRAQWQINEL
LVRKALEGRIVARLKGGDPFVFGRGGEECEALVTAGVPFEVVPGVTAGIGAAAYAGIPLTHRNVTTSVAFVTGHESPGKK
SSDIDWEGLSLGSGTVVFYMGVKSLPHITENLMAHGRRADTPVALVRWGTRPEQEVLVGTLADIADKVRRTGFKAPAITV
VGEVVRLRKTLRWFDNRPLFGRSIMVTRAADQAGEFSVMLRSLGARVLECPTIAIAPPESWDDLDSAISRIDTFDWAIFT
SFNAVRFFFQRLDAAGRDSRALGACRICVVGPKTAEALAPYGIRPDLVAADYKGEGVIEALRDVPLAGKRVLFPKGDRAR
DVIPTGLAAFGAHVEAPVAYRNAAPDALPAGTIEELEDRRIHCVTFTSSSTVQNLAAILGENRMLHLLEGVIIASIGPIT
SRSCRELGLQVHIEPQEYTLAALTAEMVRHFSAPA

Sequences:

>Translated_515_residues
MTMSTASRTNCLVYLIGAGPGDPGLITVRGRECIARADVVIYDYLANDELLSFARPDAELIYAGKVGGQHNRAQWQINEL
LVRKALEGRIVARLKGGDPFVFGRGGEECEALVTAGVPFEVVPGVTAGIGAAAYAGIPLTHRNVTTSVAFVTGHESPGKK
SSDIDWEGLSLGSGTVVFYMGVKSLPHITENLMAHGRRADTPVALVRWGTRPEQEVLVGTLADIADKVRRTGFKAPAITV
VGEVVRLRKTLRWFDNRPLFGRSIMVTRAADQAGEFSVMLRSLGARVLECPTIAIAPPESWDDLDSAISRIDTFDWAIFT
SFNAVRFFFQRLDAAGRDSRALGACRICVVGPKTAEALAPYGIRPDLVAADYKGEGVIEALRDVPLAGKRVLFPKGDRAR
DVIPTGLAAFGAHVEAPVAYRNAAPDALPAGTIEELEDRRIHCVTFTSSSTVQNLAAILGENRMLHLLEGVIIASIGPIT
SRSCRELGLQVHIEPQEYTLAALTAEMVRHFSAPA
>Mature_514_residues
TMSTASRTNCLVYLIGAGPGDPGLITVRGRECIARADVVIYDYLANDELLSFARPDAELIYAGKVGGQHNRAQWQINELL
VRKALEGRIVARLKGGDPFVFGRGGEECEALVTAGVPFEVVPGVTAGIGAAAYAGIPLTHRNVTTSVAFVTGHESPGKKS
SDIDWEGLSLGSGTVVFYMGVKSLPHITENLMAHGRRADTPVALVRWGTRPEQEVLVGTLADIADKVRRTGFKAPAITVV
GEVVRLRKTLRWFDNRPLFGRSIMVTRAADQAGEFSVMLRSLGARVLECPTIAIAPPESWDDLDSAISRIDTFDWAIFTS
FNAVRFFFQRLDAAGRDSRALGACRICVVGPKTAEALAPYGIRPDLVAADYKGEGVIEALRDVPLAGKRVLFPKGDRARD
VIPTGLAAFGAHVEAPVAYRNAAPDALPAGTIEELEDRRIHCVTFTSSSTVQNLAAILGENRMLHLLEGVIIASIGPITS
RSCRELGLQVHIEPQEYTLAALTAEMVRHFSAPA

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=244, Percent_Identity=46.7213114754098, Blast_Score=236, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6322922, Length=254, Percent_Identity=31.8897637795276, Blast_Score=114, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 55449; Mature: 55318

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMSTASRTNCLVYLIGAGPGDPGLITVRGRECIARADVVIYDYLANDELLSFARPDAEL
CCCCCCCCCCEEEEEEECCCCCCCEEEECCCHHHHHCCEEEEEECCCCHHHHHCCCCCEE
IYAGKVGGQHNRAQWQINELLVRKALEGRIVARLKGGDPFVFGRGGEECEALVTAGVPFE
EEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCEEEECCCCCCCCEEEECCCCCE
VVPGVTAGIGAAAYAGIPLTHRNVTTSVAFVTGHESPGKKSSDIDWEGLSLGSGTVVFYM
ECCCCHHCCCHHHHCCCCEECCCCEEEEEEEECCCCCCCCCCCCCCCCEECCCCCEEEEE
GVKSLPHITENLMAHGRRADTPVALVRWGTRPEQEVLVGTLADIADKVRRTGFKAPAITV
CCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHH
VGEVVRLRKTLRWFDNRPLFGRSIMVTRAADQAGEFSVMLRSLGARVLECPTIAIAPPES
HHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEECCEEEEECCCC
WDDLDSAISRIDTFDWAIFTSFNAVRFFFQRLDAAGRDSRALGACRICVVGPKTAEALAP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHCC
YGIRPDLVAADYKGEGVIEALRDVPLAGKRVLFPKGDRARDVIPTGLAAFGAHVEAPVAY
CCCCCCEEEECCCCCHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHCCCCCCCCCC
RNAAPDALPAGTIEELEDRRIHCVTFTSSSTVQNLAAILGENRMLHLLEGVIIASIGPIT
CCCCCCCCCCCCHHHHHCCEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCC
SRSCRELGLQVHIEPQEYTLAALTAEMVRHFSAPA
CCHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TMSTASRTNCLVYLIGAGPGDPGLITVRGRECIARADVVIYDYLANDELLSFARPDAEL
CCCCCCCCCEEEEEEECCCCCCCEEEECCCHHHHHCCEEEEEECCCCHHHHHCCCCCEE
IYAGKVGGQHNRAQWQINELLVRKALEGRIVARLKGGDPFVFGRGGEECEALVTAGVPFE
EEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEEEECCCEEEECCCCCCCCEEEECCCCCE
VVPGVTAGIGAAAYAGIPLTHRNVTTSVAFVTGHESPGKKSSDIDWEGLSLGSGTVVFYM
ECCCCHHCCCHHHHCCCCEECCCCEEEEEEEECCCCCCCCCCCCCCCCEECCCCCEEEEE
GVKSLPHITENLMAHGRRADTPVALVRWGTRPEQEVLVGTLADIADKVRRTGFKAPAITV
CCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHH
VGEVVRLRKTLRWFDNRPLFGRSIMVTRAADQAGEFSVMLRSLGARVLECPTIAIAPPES
HHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEECCEEEEECCCC
WDDLDSAISRIDTFDWAIFTSFNAVRFFFQRLDAAGRDSRALGACRICVVGPKTAEALAP
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHCC
YGIRPDLVAADYKGEGVIEALRDVPLAGKRVLFPKGDRARDVIPTGLAAFGAHVEAPVAY
CCCCCCEEEECCCCCHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHCCCCCCCCCC
RNAAPDALPAGTIEELEDRRIHCVTFTSSSTVQNLAAILGENRMLHLLEGVIIASIGPIT
CCCCCCCCCCCCHHHHHCCEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCC
SRSCRELGLQVHIEPQEYTLAALTAEMVRHFSAPA
CCHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]