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The map label for this gene is hisF [H]

Identifier: 39998185

GI number: 39998185

Start: 3399779

End: 3400540

Strand: Reverse

Name: hisF [H]

Synonym: GSU3095

Alternate gene names: 39998185

Gene position: 3400540-3399779 (Counterclockwise)

Preceding gene: 39998186

Following gene: 39998184

Centisome position: 89.16

GC content: 64.96

Gene sequence:

>762_bases
ATGCTGACCAAGCGGATCATCCCGTGTCTGGACGTGAAGGGCGGCCGGGTCGTCAAAGGGGTCCAGTTCCTGGAGCTGCG
TGATGCCGGCGATCCGGTTGAGATCGCCGAGCTCTATGACCGCCAGGGGGCCGACGAGCTCACGTTCCTCGACATCACCG
CCTCTTCCGACGAGCGGAGCATCATCATTGACGTGGTGCGCCGCACCGCCGAGCGGGTTTTCATGCCCCTCACCGTGGGT
GGCGGGGTGCGGACCGTGGACGATATCCGCAATCTCCTCAACGCCGGGGCCGACAAGGTGTCCATCAACACGGCGGCGGT
CCACCGGCCCGAATTCGTACGGGAAGCGGCGGAGCGCTTCGGTTCCCAGTGCACCGTGGTGGCTATCGACGCCCGGCAGG
TTCCCGGCGAGAACCGCTGGGAGGTTTACACTCACGGCGGCCGCAACCCCACCGGCATCGACGCGGTGGAGTGGGCCCGT
AGGATGGAGGAGTACGGCGCCGGCGAGATCCTCCTCACCAGCATGGACCGGGATGGCACCAAGGACGGCTATGACATCCC
CCTGACCCGCGCCATTGTCGACGCGGTCTCCATTCCGGTCATCGCCTCCGGCGGGGTGGGAAACCTTGAGCATCTGTACG
ACGGCTTCGTCAAGGCAGGGGCAAGCGCCTGTCTTGCTGCGTCCATCTTTCACTATAAGGAGTACACCATCGGCGAGGCC
AAGGAGTATCTCCGTCAACGGGGGGTTCCGGTCCGGTTATGA

Upstream 100 bases:

>100_bases
ACGAGCGGCATCGCCGGCGCCATCACCGGCAAGGCGGTCTACACCGGGGCCATCAATCTGGCTGAAGCGGTAGCACTCAC
GAAGCGGGGAGGGGCCTGAG

Downstream 100 bases:

>100_bases
ACGAGCGTGACGACATCCTGCAGGCAGTCTATGAGGTGATTCTCGACCGGAAAGGTGCTCTGCCCGAGAGCTCCTATACG
GCGTCCCTCTTTCACAAGGG

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MLTKRIIPCLDVKGGRVVKGVQFLELRDAGDPVEIAELYDRQGADELTFLDITASSDERSIIIDVVRRTAERVFMPLTVG
GGVRTVDDIRNLLNAGADKVSINTAAVHRPEFVREAAERFGSQCTVVAIDARQVPGENRWEVYTHGGRNPTGIDAVEWAR
RMEEYGAGEILLTSMDRDGTKDGYDIPLTRAIVDAVSIPVIASGGVGNLEHLYDGFVKAGASACLAASIFHYKEYTIGEA
KEYLRQRGVPVRL

Sequences:

>Translated_253_residues
MLTKRIIPCLDVKGGRVVKGVQFLELRDAGDPVEIAELYDRQGADELTFLDITASSDERSIIIDVVRRTAERVFMPLTVG
GGVRTVDDIRNLLNAGADKVSINTAAVHRPEFVREAAERFGSQCTVVAIDARQVPGENRWEVYTHGGRNPTGIDAVEWAR
RMEEYGAGEILLTSMDRDGTKDGYDIPLTRAIVDAVSIPVIASGGVGNLEHLYDGFVKAGASACLAASIFHYKEYTIGEA
KEYLRQRGVPVRL
>Mature_253_residues
MLTKRIIPCLDVKGGRVVKGVQFLELRDAGDPVEIAELYDRQGADELTFLDITASSDERSIIIDVVRRTAERVFMPLTVG
GGVRTVDDIRNLLNAGADKVSINTAAVHRPEFVREAAERFGSQCTVVAIDARQVPGENRWEVYTHGGRNPTGIDAVEWAR
RMEEYGAGEILLTSMDRDGTKDGYDIPLTRAIVDAVSIPVIASGGVGNLEHLYDGFVKAGASACLAASIFHYKEYTIGEA
KEYLRQRGVPVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=42.4124513618677, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI87082028, Length=211, Percent_Identity=31.2796208530806, Blast_Score=91, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=36.3057324840764, Blast_Score=179, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27707; Mature: 27707

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKRIIPCLDVKGGRVVKGVQFLELRDAGDPVEIAELYDRQGADELTFLDITASSDERS
CCCCCCCCEECCCCCEEECCEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCH
IIIDVVRRTAERVFMPLTVGGGVRTVDDIRNLLNAGADKVSINTAAVHRPEFVREAAERF
HHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEEECHHHCCCHHHHHHHHHHH
GSQCTVVAIDARQVPGENRWEVYTHGGRNPTGIDAVEWARRMEEYGAGEILLTSMDRDGT
CCCEEEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
KDGYDIPLTRAIVDAVSIPVIASGGVGNLEHLYDGFVKAGASACLAASIFHYKEYTIGEA
CCCCCCCHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHH
KEYLRQRGVPVRL
HHHHHHCCCCCCC
>Mature Secondary Structure
MLTKRIIPCLDVKGGRVVKGVQFLELRDAGDPVEIAELYDRQGADELTFLDITASSDERS
CCCCCCCCEECCCCCEEECCEEEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCH
IIIDVVRRTAERVFMPLTVGGGVRTVDDIRNLLNAGADKVSINTAAVHRPEFVREAAERF
HHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEEECHHHCCCHHHHHHHHHHH
GSQCTVVAIDARQVPGENRWEVYTHGGRNPTGIDAVEWARRMEEYGAGEILLTSMDRDGT
CCCEEEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
KDGYDIPLTRAIVDAVSIPVIASGGVGNLEHLYDGFVKAGASACLAASIFHYKEYTIGEA
CCCCCCCHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHH
KEYLRQRGVPVRL
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA