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The map label for this gene is cobB [H]

Identifier: 39998177

GI number: 39998177

Start: 3392958

End: 3393785

Strand: Reverse

Name: cobB [H]

Synonym: GSU3087

Alternate gene names: 39998177

Gene position: 3393785-3392958 (Counterclockwise)

Preceding gene: 39998178

Following gene: 39998176

Centisome position: 88.98

GC content: 61.35

Gene sequence:

>828_bases
ATGGAACTGCGTGAGCGATTCCTGCGTGCGGCCGAAGCGCTGCGGCGGGCCGAAGTGCTGGTCATCACTTCAGGGGCCGG
CATGGGTGTCGATTCGGGGCTGCCCGACTTCCGAGGGGATAGCGGTTTCTGGAAGGCGTATCCGCCCTATGAACGGCTCG
GCATTACCTTCGTTGGCGCCGCCAACCCGGCTCATTTTGAGAAAGATCCCGCCTTTGGCTGGGGATTCTACGGCCATCGG
ACCAACCTCTACCGTGCCACAGTCCCTCACGCCGGGTTCGGAATCATCAGGGCATGGATCGAACGCTACGGCCTCGACCA
TTTCGTGGTGACCTCCAACGTGGACGGCCAGTTCCAGAAGGCCGGGTTCGCCGAAGACCGTATTCTGGAAGTGCACGGTT
CCATTCACCATCTCCAGTGCACCAAACCATGCACAATGGCTGTTTGGGAGAACCGGGAAACCATTCCCGTTGACGAGTCG
ACCATGCGGGCGGGACACATTCCCCGCTGCATCCACTGCGGCGACGTGGCGCGACCCAATATCCTCATGTTCGGGGACTA
TGGCTGGATCAGCGGACGAACCGCCCGGCAGCAGCGGAACTTCGACGAGTTTCTGACTGAAGTCAGAGGCCGGCGCGCGG
TGGTGGTGGAGCTGGGGGCAGGAACGGCAATTCCCACAATTCGCTACCTGACGGAAGAAGTCGGCTCCCTTCACCAGGCC
GTGGCAATCCGGATCAATCCCCGGGAGGCTCAGATCGGCGCCCCCCATATTTCTCTCGCATGCGGCGCCCTTGAAGGGCT
TGCCGGCATCAAATCCTGTCTGGAGTAA

Upstream 100 bases:

>100_bases
TAGGACTCGACCCGTGCGAGGCGTGCCGCCTGCTTGATCCCATCCTCGTGCGGGTGGCCATCTGACGTTCGGATCAACTG
GGTGTCGCGGGAGGTGATCC

Downstream 100 bases:

>100_bases
TTCATGTCGTCGGTTATTGTGCGCAAACGTACCGCAGTCACTCCCCTTTCGGGCGAGCATGCCTTCTTCGCCACCACAGC
CAAGGGGGTTGAGGATGTCC

Product: Sir2 family transcriptional regulator

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog; ssSir2 [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MELRERFLRAAEALRRAEVLVITSGAGMGVDSGLPDFRGDSGFWKAYPPYERLGITFVGAANPAHFEKDPAFGWGFYGHR
TNLYRATVPHAGFGIIRAWIERYGLDHFVVTSNVDGQFQKAGFAEDRILEVHGSIHHLQCTKPCTMAVWENRETIPVDES
TMRAGHIPRCIHCGDVARPNILMFGDYGWISGRTARQQRNFDEFLTEVRGRRAVVVELGAGTAIPTIRYLTEEVGSLHQA
VAIRINPREAQIGAPHISLACGALEGLAGIKSCLE

Sequences:

>Translated_275_residues
MELRERFLRAAEALRRAEVLVITSGAGMGVDSGLPDFRGDSGFWKAYPPYERLGITFVGAANPAHFEKDPAFGWGFYGHR
TNLYRATVPHAGFGIIRAWIERYGLDHFVVTSNVDGQFQKAGFAEDRILEVHGSIHHLQCTKPCTMAVWENRETIPVDES
TMRAGHIPRCIHCGDVARPNILMFGDYGWISGRTARQQRNFDEFLTEVRGRRAVVVELGAGTAIPTIRYLTEEVGSLHQA
VAIRINPREAQIGAPHISLACGALEGLAGIKSCLE
>Mature_275_residues
MELRERFLRAAEALRRAEVLVITSGAGMGVDSGLPDFRGDSGFWKAYPPYERLGITFVGAANPAHFEKDPAFGWGFYGHR
TNLYRATVPHAGFGIIRAWIERYGLDHFVVTSNVDGQFQKAGFAEDRILEVHGSIHHLQCTKPCTMAVWENRETIPVDES
TMRAGHIPRCIHCGDVARPNILMFGDYGWISGRTARQQRNFDEFLTEVRGRRAVVVELGAGTAIPTIRYLTEEVGSLHQA
VAIRINPREAQIGAPHISLACGALEGLAGIKSCLE

Specific function: Probably has a role in chromatin modulation by deacetylation of the N-terminal lysine residue of Alba, the major archaeal chromatin protein and that, in turn, increases Alba's DNA binding affinity, thereby repressing transcription [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI13787215, Length=187, Percent_Identity=28.8770053475936, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI6912664, Length=187, Percent_Identity=28.8770053475936, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI300795542, Length=176, Percent_Identity=31.8181818181818, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24648389, Length=171, Percent_Identity=30.9941520467836, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 30431; Mature: 30431

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRERFLRAAEALRRAEVLVITSGAGMGVDSGLPDFRGDSGFWKAYPPYERLGITFVGA
CCHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEEC
ANPAHFEKDPAFGWGFYGHRTNLYRATVPHAGFGIIRAWIERYGLDHFVVTSNVDGQFQK
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHH
AGFAEDRILEVHGSIHHLQCTKPCTMAVWENRETIPVDESTMRAGHIPRCIHCGDVARPN
CCCCCCCEEEECCCEEEEEECCCCEEEEECCCCCCCCCHHHHHCCCCCCEEECCCCCCCC
ILMFGDYGWISGRTARQQRNFDEFLTEVRGRRAVVVELGAGTAIPTIRYLTEEVGSLHQA
EEEEECCCCCCCCHHHHHCCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHCEE
VAIRINPREAQIGAPHISLACGALEGLAGIKSCLE
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELRERFLRAAEALRRAEVLVITSGAGMGVDSGLPDFRGDSGFWKAYPPYERLGITFVGA
CCHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEEC
ANPAHFEKDPAFGWGFYGHRTNLYRATVPHAGFGIIRAWIERYGLDHFVVTSNVDGQFQK
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEEECCCCCCHHH
AGFAEDRILEVHGSIHHLQCTKPCTMAVWENRETIPVDESTMRAGHIPRCIHCGDVARPN
CCCCCCCEEEECCCEEEEEECCCCEEEEECCCCCCCCCHHHHHCCCCCCEEECCCCCCCC
ILMFGDYGWISGRTARQQRNFDEFLTEVRGRRAVVVELGAGTAIPTIRYLTEEVGSLHQA
EEEEECCCCCCCCHHHHHCCHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHCEE
VAIRINPREAQIGAPHISLACGALEGLAGIKSCLE
EEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726; 11935028 [H]