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The map label for this gene is murE

Identifier: 39998164

GI number: 39998164

Start: 3381149

End: 3382678

Strand: Reverse

Name: murE

Synonym: GSU3074

Alternate gene names: 39998164

Gene position: 3382678-3381149 (Counterclockwise)

Preceding gene: 39998165

Following gene: 39998163

Centisome position: 88.69

GC content: 62.48

Gene sequence:

>1530_bases
ATGCGACTAGAAGATCTGGCACGGGTTGTTGATCCCATCGCGGTACGGGGGGATCTGACGCGGGAGATCAACGGGCTCTA
CTGCGATTCACGGCAGGTGAGGTCCGGTGGTCTCTTTTTCGCGTTAAAAGGGGTCGCGTCCGACGGGCATGACTTTATTG
CGTCAGCCCGCGAACGGGGCGCCGTCGCCGTTGTGCTGGAGGACGAAACGCGAGCACCCTGCGGGATGGAATGGATCCGG
GTCGGGGATGCGCGCCTCGCCATGTCGCGTATGGCGGCGCTTTTTTACGGACAGCCCACCGACGGCGTACCGGTCGTGGG
CATTACCGGTACCAACGGCAAGACCACCACCACCTATCTGGTCGAGGCGATCATGTCCCGGGCCGGCATCCCGGCGGCAG
TGCTCGGAACCATCAGCTACCGCTTCGGCTCGAAGCTCGTTCCCGCACCTCACACCACGCCGGAATCCGTTGAGTTGCAG
GCAACAATCCGTGATCTGGTGGATGAGGGGGCGAAGGCTGTGGTAATGGAGGTTTCGTCCCACGCCCTGGAACAGCGGCG
GGTAGATAGCTGCCGTTTCGATGTGGCGGTCTTCACGAATCTGACGCGTGACCACCTGGACTATCACCGGGACATGGAGT
CCTATTTCGGCAGCAAGGCGCGGCTCTTTACGGAGTTGGTTGCGCCCGACGGGGTCAAGCCGCGGCGCGCAGCCGCCATC
AACAGGGATGACTCCTACGGTGCCCGGCTGGTCGAGACTGCCGTCGCGCCGGTGATTTCCTACGGACTCGCGGCCGATGC
AGCCGTGCGAGCCGAAAACGTTGTCTTCTCTGTGGACGGCATTGCGGGGACCCTGGTCACTCCGTTCGGGACGGCCCCGT
TTCACTCGCACCTGCTGGGACGGTTCAACCTGTATAACATCCTCGCCGCCGTTGCCGCGGGGGTCGGCCTCGGGCTTTCC
CTTGACGTCATTCTGGGGGGAATCGAGGGTGATGTCCGGGTCCCTGGACGGCTGGAGCGGGTTCACAATGAGCGCGGCGT
AACGGTCCTGGTTGACTACGCCCACACTGGCGATGCCCTGGAGAATGTTCTGAAGACCGTGTCGGAGCTTGCGACAGGGC
GGATTATTACTGTCTTCGGCTGCGGCGGCGACCGCGACCGCGGCAAACGGCCCGTGATGGCGGAAATCAGCGGACGCTAC
AGCCACCTGACCATCGTTACTTCCGACAACCCCAGGACCGAGGAGCCCGCGGCGATCATCAAGGAGGTTCTGACCGGAAT
CATACCCATGGGATTGCGGGAATACGATGCCCGTGAACTGAACCGGGGCTTTGTGGAGAAGGGGTTCACTTCGCTGGTAT
CCCGTCACGACGCTATCCGGCTTGCTGCAACGGTTGCCGTGGCGGGCGACATCGTGTTGCTGGCGGGCAAGGGGCACGAG
GACTACCAGATCATCGGAACGGAAAAATTCCACTTTGACGATCGCGAAGAGGCGATCGCCGCATTCCGCTCATCGGACAG
CGGAGAATAG

Upstream 100 bases:

>100_bases
AGCCCGCTTCCCGGTCACAGGGTTGGGCCGTCGGATCAGGTGTGGGTGAAGTTCGTACCATCGGCATGACGCCATCAAAT
ACGCTCAATGGCGAGGAACC

Downstream 100 bases:

>100_bases
GGTTGGACGGGGACGTGCTGTTTACGCTCGATGACATACTGGGGGCTACAGGCGGGGAGATCGTGGCGGGAGGCGAGACA
ATCGGAGCCTCCGGCGTTTC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MRLEDLARVVDPIAVRGDLTREINGLYCDSRQVRSGGLFFALKGVASDGHDFIASARERGAVAVVLEDETRAPCGMEWIR
VGDARLAMSRMAALFYGQPTDGVPVVGITGTNGKTTTTYLVEAIMSRAGIPAAVLGTISYRFGSKLVPAPHTTPESVELQ
ATIRDLVDEGAKAVVMEVSSHALEQRRVDSCRFDVAVFTNLTRDHLDYHRDMESYFGSKARLFTELVAPDGVKPRRAAAI
NRDDSYGARLVETAVAPVISYGLAADAAVRAENVVFSVDGIAGTLVTPFGTAPFHSHLLGRFNLYNILAAVAAGVGLGLS
LDVILGGIEGDVRVPGRLERVHNERGVTVLVDYAHTGDALENVLKTVSELATGRIITVFGCGGDRDRGKRPVMAEISGRY
SHLTIVTSDNPRTEEPAAIIKEVLTGIIPMGLREYDARELNRGFVEKGFTSLVSRHDAIRLAATVAVAGDIVLLAGKGHE
DYQIIGTEKFHFDDREEAIAAFRSSDSGE

Sequences:

>Translated_509_residues
MRLEDLARVVDPIAVRGDLTREINGLYCDSRQVRSGGLFFALKGVASDGHDFIASARERGAVAVVLEDETRAPCGMEWIR
VGDARLAMSRMAALFYGQPTDGVPVVGITGTNGKTTTTYLVEAIMSRAGIPAAVLGTISYRFGSKLVPAPHTTPESVELQ
ATIRDLVDEGAKAVVMEVSSHALEQRRVDSCRFDVAVFTNLTRDHLDYHRDMESYFGSKARLFTELVAPDGVKPRRAAAI
NRDDSYGARLVETAVAPVISYGLAADAAVRAENVVFSVDGIAGTLVTPFGTAPFHSHLLGRFNLYNILAAVAAGVGLGLS
LDVILGGIEGDVRVPGRLERVHNERGVTVLVDYAHTGDALENVLKTVSELATGRIITVFGCGGDRDRGKRPVMAEISGRY
SHLTIVTSDNPRTEEPAAIIKEVLTGIIPMGLREYDARELNRGFVEKGFTSLVSRHDAIRLAATVAVAGDIVLLAGKGHE
DYQIIGTEKFHFDDREEAIAAFRSSDSGE
>Mature_509_residues
MRLEDLARVVDPIAVRGDLTREINGLYCDSRQVRSGGLFFALKGVASDGHDFIASARERGAVAVVLEDETRAPCGMEWIR
VGDARLAMSRMAALFYGQPTDGVPVVGITGTNGKTTTTYLVEAIMSRAGIPAAVLGTISYRFGSKLVPAPHTTPESVELQ
ATIRDLVDEGAKAVVMEVSSHALEQRRVDSCRFDVAVFTNLTRDHLDYHRDMESYFGSKARLFTELVAPDGVKPRRAAAI
NRDDSYGARLVETAVAPVISYGLAADAAVRAENVVFSVDGIAGTLVTPFGTAPFHSHLLGRFNLYNILAAVAAGVGLGLS
LDVILGGIEGDVRVPGRLERVHNERGVTVLVDYAHTGDALENVLKTVSELATGRIITVFGCGGDRDRGKRPVMAEISGRY
SHLTIVTSDNPRTEEPAAIIKEVLTGIIPMGLREYDARELNRGFVEKGFTSLVSRHDAIRLAATVAVAGDIVLLAGKGHE
DYQIIGTEKFHFDDREEAIAAFRSSDSGE

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=489, Percent_Identity=37.4233128834356, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1786274, Length=363, Percent_Identity=26.9972451790634, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_GEOSL (Q748D2)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_954115.1
- HSSP:   P22188
- ProteinModelPortal:   Q748D2
- SMR:   Q748D2
- GeneID:   2686302
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU3074
- NMPDR:   fig|243231.1.peg.3053
- TIGR:   GSU3074
- HOGENOM:   HBG602753
- OMA:   VLGCGGD
- ProtClustDB:   PRK00139
- BioCyc:   GSUL243231:GSU_3074-MONOMER
- BRENDA:   6.3.2.13
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 54779; Mature: 54779

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: BINDING 30-30 BINDING 179-179 BINDING 185-185 BINDING 187-187 BINDING 385-385 BINDING 476-476 BINDING 480-480

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLEDLARVVDPIAVRGDLTREINGLYCDSRQVRSGGLFFALKGVASDGHDFIASARERG
CCHHHHHHHHCCHHHCCCHHHHCCCEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHCCC
AVAVVLEDETRAPCGMEWIRVGDARLAMSRMAALFYGQPTDGVPVVGITGTNGKTTTTYL
CEEEEEECCCCCCCCCHHEEECHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHH
VEAIMSRAGIPAAVLGTISYRFGSKLVPAPHTTPESVELQATIRDLVDEGAKAVVMEVSS
HHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCEEEHHHHH
HALEQRRVDSCRFDVAVFTNLTRDHLDYHRDMESYFGSKARLFTELVAPDGVKPRRAAAI
HHHHHHHHHHHHEEHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHCC
NRDDSYGARLVETAVAPVISYGLAADAAVRAENVVFSVDGIAGTLVTPFGTAPFHSHLLG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCCCCHHHHHHH
RFNLYNILAAVAAGVGLGLSLDVILGGIEGDVRVPGRLERVHNERGVTVLVDYAHTGDAL
HHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEECCHHHHHHHCCCCCEEEEEECCCCHHH
ENVLKTVSELATGRIITVFGCGGDRDRGKRPVMAEISGRYSHLTIVTSDNPRTEEPAAII
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCCCHHHHH
KEVLTGIIPMGLREYDARELNRGFVEKGFTSLVSRHDAIRLAATVAVAGDIVLLAGKGHE
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHEEECCEEEEECCCCC
DYQIIGTEKFHFDDREEAIAAFRSSDSGE
CEEEEECEEECCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRLEDLARVVDPIAVRGDLTREINGLYCDSRQVRSGGLFFALKGVASDGHDFIASARERG
CCHHHHHHHHCCHHHCCCHHHHCCCEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHCCC
AVAVVLEDETRAPCGMEWIRVGDARLAMSRMAALFYGQPTDGVPVVGITGTNGKTTTTYL
CEEEEEECCCCCCCCCHHEEECHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHH
VEAIMSRAGIPAAVLGTISYRFGSKLVPAPHTTPESVELQATIRDLVDEGAKAVVMEVSS
HHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCCEEEHHHHH
HALEQRRVDSCRFDVAVFTNLTRDHLDYHRDMESYFGSKARLFTELVAPDGVKPRRAAAI
HHHHHHHHHHHHEEHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHCC
NRDDSYGARLVETAVAPVISYGLAADAAVRAENVVFSVDGIAGTLVTPFGTAPFHSHLLG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCCCCCCHHHHHHH
RFNLYNILAAVAAGVGLGLSLDVILGGIEGDVRVPGRLERVHNERGVTVLVDYAHTGDAL
HHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEECCHHHHHHHCCCCCEEEEEECCCCHHH
ENVLKTVSELATGRIITVFGCGGDRDRGKRPVMAEISGRYSHLTIVTSDNPRTEEPAAII
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCCCHHHHH
KEVLTGIIPMGLREYDARELNRGFVEKGFTSLVSRHDAIRLAATVAVAGDIVLLAGKGHE
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHEEECCEEEEECCCCC
DYQIIGTEKFHFDDREEAIAAFRSSDSGE
CEEEEECEEECCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA