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The map label for this gene is epsM [H]

Identifier: 39998111

GI number: 39998111

Start: 3314633

End: 3315748

Strand: Direct

Name: epsM [H]

Synonym: GSU3020

Alternate gene names: 39998111

Gene position: 3314633-3315748 (Clockwise)

Preceding gene: 39998110

Following gene: 39998112

Centisome position: 86.9

GC content: 49.37

Gene sequence:

>1116_bases
ATGATGAAAACAATGGACATAATCATTCCCAGAGAATTTGTCAGCGACGACCAGTACCTGCTCCAGAAACTCTGTGTCGT
GAACAATGACTTTGTAAAAGAAGGAGAGATACTTGCCCTGATCGAATCATCAAAATCTGTCATTGACGTTACGAGTCCGG
CTGATGGCTATGTCTATTTTTTCTCTGACGAAGGAGACATTGTGAGTGTCGGCGAGAGGTTGGCCTTGCTCGCATCAACC
AAGGAGGCATTACAGGTTGAAATAGAGAATTCCAACAAGAACTCTCAGCGTCGCAAGGAGAGTGAGACAAAGAGCGAGGA
CGTTTCATTGTCTGGCGTGAGGTGCTCCAAGAAGGCATTGCTTCTGATGAAACAGCACAATATCGATGTAGGAGCATTTG
ATGGTCTCGGGATGGTTACGGCGCAGGACGTAGAGCACTATCTCTCCAGCAGGGAAAAAGCCGTTAAAGCGACAGTAGCT
CCATCTTCCGTAAATAGGCAGAAAATCATCATCCTTGGTGGCGGAGGACATTCAAAAGTATGCATAGACATACTGCGCCA
GGCACAATCTTTCACAATCGCAGGAATTCTCGACTCTATCCAGGACATTGGCGCAGAAGTGCTGGGAATCCCGGTCATTG
GAAGAGACACAATGCCGGAACTACTCAAGACCAGAGAGAGTGGCATCTCCCTTGCGGTTAACGGGATTGGACTCATTCCG
GATCACCGGAACAGATGCAAGCTTTTTGAGAGGCTATTGGAGGCCGGCTTTCATCTCCCTAACCTCATACACCCCAAGGC
ATCAATCGAACCTTCGGCAAAACTCGGCGAAGGGAACCAGATCATGGCAGGAGCCATTATCGGGAGCGATGTCACAGTAG
GAAACTACTGTCTCATAAACTCGGGAGTCGTCGTCTCGCACGACTGTATCATCGACGACCACGTCCACCTGGCCCCCGGT
GCGCTGCTTGCAGGAGCAGTCAGAGTTGGAAGAAACTCTTTGATCGGCATGGGCGTTACAATCTACGCAAAAGTAACAAT
AGGAAGCAACGTAGTTATAGCCAACGGCGCCAACGTGTTTCACGATGTGCCGGACAACACCGTCGTCAAGATTTGA

Upstream 100 bases:

>100_bases
GTATCGGTTACGACTCTACCATTCCATCATCAGCCAGCCGTGAATCCAGATTGATAACAAATGCCGAATCAATCTTTGAA
CGTATTGTGGAGATTTTCAA

Downstream 100 bases:

>100_bases
CGTAATGGTTGCCAAATGAATATGAACCCTAATGCAAGTTTCGACAATATGACTGAATCCAGCGCCCCCCCTTCATTCAA
GGAAAAACTCCACGTTGGGC

Product: hexapeptide transferase

Products: CoA; acetylmaltose [C]

Alternate protein names: NA

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MMKTMDIIIPREFVSDDQYLLQKLCVVNNDFVKEGEILALIESSKSVIDVTSPADGYVYFFSDEGDIVSVGERLALLAST
KEALQVEIENSNKNSQRRKESETKSEDVSLSGVRCSKKALLLMKQHNIDVGAFDGLGMVTAQDVEHYLSSREKAVKATVA
PSSVNRQKIIILGGGGHSKVCIDILRQAQSFTIAGILDSIQDIGAEVLGIPVIGRDTMPELLKTRESGISLAVNGIGLIP
DHRNRCKLFERLLEAGFHLPNLIHPKASIEPSAKLGEGNQIMAGAIIGSDVTVGNYCLINSGVVVSHDCIIDDHVHLAPG
ALLAGAVRVGRNSLIGMGVTIYAKVTIGSNVVIANGANVFHDVPDNTVVKI

Sequences:

>Translated_371_residues
MMKTMDIIIPREFVSDDQYLLQKLCVVNNDFVKEGEILALIESSKSVIDVTSPADGYVYFFSDEGDIVSVGERLALLAST
KEALQVEIENSNKNSQRRKESETKSEDVSLSGVRCSKKALLLMKQHNIDVGAFDGLGMVTAQDVEHYLSSREKAVKATVA
PSSVNRQKIIILGGGGHSKVCIDILRQAQSFTIAGILDSIQDIGAEVLGIPVIGRDTMPELLKTRESGISLAVNGIGLIP
DHRNRCKLFERLLEAGFHLPNLIHPKASIEPSAKLGEGNQIMAGAIIGSDVTVGNYCLINSGVVVSHDCIIDDHVHLAPG
ALLAGAVRVGRNSLIGMGVTIYAKVTIGSNVVIANGANVFHDVPDNTVVKI
>Mature_371_residues
MMKTMDIIIPREFVSDDQYLLQKLCVVNNDFVKEGEILALIESSKSVIDVTSPADGYVYFFSDEGDIVSVGERLALLAST
KEALQVEIENSNKNSQRRKESETKSEDVSLSGVRCSKKALLLMKQHNIDVGAFDGLGMVTAQDVEHYLSSREKAVKATVA
PSSVNRQKIIILGGGGHSKVCIDILRQAQSFTIAGILDSIQDIGAEVLGIPVIGRDTMPELLKTRESGISLAVNGIGLIP
DHRNRCKLFERLLEAGFHLPNLIHPKASIEPSAKLGEGNQIMAGAIIGSDVTVGNYCLINSGVVVSHDCIIDDHVHLAPG
ALLAGAVRVGRNSLIGMGVTIYAKVTIGSNVVIANGANVFHDVPDNTVVKI

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR013817
- InterPro:   IPR020019
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 39790; Mature: 39790

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00101 HEXAPEP_TRANSFERASES ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKTMDIIIPREFVSDDQYLLQKLCVVNNDFVKEGEILALIESSKSVIDVTSPADGYVYF
CCCCEEEEECHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEECCCCCEEEE
FSDEGDIVSVGERLALLASTKEALQVEIENSNKNSQRRKESETKSEDVSLSGVRCSKKAL
ECCCCCEEEHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHCCCCCCEEECCEECCCEE
LLMKQHNIDVGAFDGLGMVTAQDVEHYLSSREKAVKATVAPSSVNRQKIIILGGGGHSKV
EEEEECCCCEECCCCCCCEEHHHHHHHHHCHHHHEEEEECCCCCCCEEEEEEECCCCCHH
CIDILRQAQSFTIAGILDSIQDIGAEVLGIPVIGRDTMPELLKTRESGISLAVNGIGLIP
HHHHHHHHHCEEHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEECEEECC
DHRNRCKLFERLLEAGFHLPNLIHPKASIEPSAKLGEGNQIMAGAIIGSDVTVGNYCLIN
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEECCEEEEE
SGVVVSHDCIIDDHVHLAPGALLAGAVRVGRNSLIGMGVTIYAKVTIGSNVVIANGANVF
CCEEEEECEEEECCCEECCCHHHHHHHHHCCCCEEECCEEEEEEEEECCEEEEECCCCEE
HDVPDNTVVKI
ECCCCCCEEEC
>Mature Secondary Structure
MMKTMDIIIPREFVSDDQYLLQKLCVVNNDFVKEGEILALIESSKSVIDVTSPADGYVYF
CCCCEEEEECHHHCCCHHHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEECCCCCEEEE
FSDEGDIVSVGERLALLASTKEALQVEIENSNKNSQRRKESETKSEDVSLSGVRCSKKAL
ECCCCCEEEHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHCCCCCCEEECCEECCCEE
LLMKQHNIDVGAFDGLGMVTAQDVEHYLSSREKAVKATVAPSSVNRQKIIILGGGGHSKV
EEEEECCCCEECCCCCCCEEHHHHHHHHHCHHHHEEEEECCCCCCCEEEEEEECCCCCHH
CIDILRQAQSFTIAGILDSIQDIGAEVLGIPVIGRDTMPELLKTRESGISLAVNGIGLIP
HHHHHHHHHCEEHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEECEEECC
DHRNRCKLFERLLEAGFHLPNLIHPKASIEPSAKLGEGNQIMAGAIIGSDVTVGNYCLIN
CCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCEECCEEEEE
SGVVVSHDCIIDDHVHLAPGALLAGAVRVGRNSLIGMGVTIYAKVTIGSNVVIANGANVF
CCEEEEECEEEECCCEECCCHHHHHHHHHCCCCEEECCEEEEEEEEECCEEEEECCCCEE
HDVPDNTVVKI
ECCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 90 {maltose}} 62 {glucose}} 0.018 {acetyl-CoA}} 40 {triethanolamine}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]