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The map label for this gene is cobS [H]

Identifier: 39998099

GI number: 39998099

Start: 3300552

End: 3301292

Strand: Reverse

Name: cobS [H]

Synonym: GSU3008

Alternate gene names: 39998099

Gene position: 3301292-3300552 (Counterclockwise)

Preceding gene: 39998100

Following gene: 39998098

Centisome position: 86.55

GC content: 68.02

Gene sequence:

>741_bases
TTGAAACTCTATTTCATCGCCCTTCAGTTTCTGACCGTCGTCCCGCTGCCGTTCCGGCTGCGCTGGGACGAGCGCGCCCT
GGGACGCTCCATGGCATTCTTCCCCCTGGCGGGGCTCACTCTGGGAACACTGCTGGCCGGGGCCGACATGCTTTTCGCAG
CGCTGGTGCCAAGGCCCGTCAGCGATCTCCTGCTGGTGGCGCTGCTGGCCGGGGTAACCGGTGCGTTGCACCTGGACGGT
CTGGCCGATGTCTGCGACGGGCTGGCGGCTCGTGGCGGCAGGGAGCGTTTCCTGGCGGTCATGAAGGATCCCCGGGTGGG
AGCCGTGGGGGTAGTGGGGCTCGTGCTCGGGCTGGCGCTCAAGTACCAGGCCCTGCTGGCGGTGCCGGACGACCTCAAGC
GTCAGGCGCTCTTGCTCTTCCCTGCCGTGGCCCGCTACGCCCAGGTGCAGATGACCGTCGGCGCCAACCGCGCCCGACAG
GATGGCCTCGGCGCCGCTTTCATCGCCGGGACCGGGATGTTCCAGCTGGTGACGGCCGGCACGATCACGGCCGTTGCAGC
CTGGTTGCTGCTGGGCCCGGCGGGCATGGCCGTGCTTGCGGCATCCGTTCTTTTCACCGGCGGGTTCCGGTGGTGGTTCC
ATCGGCATCTCGGCGGGGTTACCGGCGATGTCATCGGCTGCGCCAGCGAACTGAACGAAATTCTCTGCCTGCTGGTCATC
CTGGCCCTGCACCGGGGCTGA

Upstream 100 bases:

>100_bases
GGACTGGTGGGGCACTGGCCATGGGACTCATCGAGGCCGGAGTCAGAATTTTGACGGAAATGGCCACCTTTGAGGAAGCG
GGCGTGGCGGAAGGGGACTA

Downstream 100 bases:

>100_bases
CAGCCCCGTACGGAGACTGTCCTTCCATACTTCGATTCCTTGCCGGGAACACACAGGATGTAGAGGCAGGGGCTATGCCG
TGAGGCTGCCCGAACAAACA

Product: cobalamin 5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKLYFIALQFLTVVPLPFRLRWDERALGRSMAFFPLAGLTLGTLLAGADMLFAALVPRPVSDLLLVALLAGVTGALHLDG
LADVCDGLAARGGRERFLAVMKDPRVGAVGVVGLVLGLALKYQALLAVPDDLKRQALLLFPAVARYAQVQMTVGANRARQ
DGLGAAFIAGTGMFQLVTAGTITAVAAWLLLGPAGMAVLAASVLFTGGFRWWFHRHLGGVTGDVIGCASELNEILCLLVI
LALHRG

Sequences:

>Translated_246_residues
MKLYFIALQFLTVVPLPFRLRWDERALGRSMAFFPLAGLTLGTLLAGADMLFAALVPRPVSDLLLVALLAGVTGALHLDG
LADVCDGLAARGGRERFLAVMKDPRVGAVGVVGLVLGLALKYQALLAVPDDLKRQALLLFPAVARYAQVQMTVGANRARQ
DGLGAAFIAGTGMFQLVTAGTITAVAAWLLLGPAGMAVLAASVLFTGGFRWWFHRHLGGVTGDVIGCASELNEILCLLVI
LALHRG
>Mature_246_residues
MKLYFIALQFLTVVPLPFRLRWDERALGRSMAFFPLAGLTLGTLLAGADMLFAALVPRPVSDLLLVALLAGVTGALHLDG
LADVCDGLAARGGRERFLAVMKDPRVGAVGVVGLVLGLALKYQALLAVPDDLKRQALLLFPAVARYAQVQMTVGANRARQ
DGLGAAFIAGTGMFQLVTAGTITAVAAWLLLGPAGMAVLAASVLFTGGFRWWFHRHLGGVTGDVIGCASELNEILCLLVI
LALHRG

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=247, Percent_Identity=34.8178137651822, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 25928; Mature: 25928

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYFIALQFLTVVPLPFRLRWDERALGRSMAFFPLAGLTLGTLLAGADMLFAALVPRPV
CCHHHHHHHHHHHCCCCCEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
SDLLLVALLAGVTGALHLDGLADVCDGLAARGGRERFLAVMKDPRVGAVGVVGLVLGLAL
HHHHHHHHHHHCCHHHHHCCHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHH
KYQALLAVPDDLKRQALLLFPAVARYAQVQMTVGANRARQDGLGAAFIAGTGMFQLVTAG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHCCCCCHHHHCCCHHHHHHHH
TITAVAAWLLLGPAGMAVLAASVLFTGGFRWWFHRHLGGVTGDVIGCASELNEILCLLVI
HHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LALHRG
HHHHCC
>Mature Secondary Structure
MKLYFIALQFLTVVPLPFRLRWDERALGRSMAFFPLAGLTLGTLLAGADMLFAALVPRPV
CCHHHHHHHHHHHCCCCCEECCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
SDLLLVALLAGVTGALHLDGLADVCDGLAARGGRERFLAVMKDPRVGAVGVVGLVLGLAL
HHHHHHHHHHHCCHHHHHCCHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHH
KYQALLAVPDDLKRQALLLFPAVARYAQVQMTVGANRARQDGLGAAFIAGTGMFQLVTAG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHCCCCCHHHHCCCHHHHHHHH
TITAVAAWLLLGPAGMAVLAASVLFTGGFRWWFHRHLGGVTGDVIGCASELNEILCLLVI
HHHHHHHHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LALHRG
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA