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The map label for this gene is pilR [H]

Identifier: 39997787

GI number: 39997787

Start: 2971392

End: 2971778

Strand: Reverse

Name: pilR [H]

Synonym: GSU2693

Alternate gene names: 39997787

Gene position: 2971778-2971392 (Counterclockwise)

Preceding gene: 39997789

Following gene: 39997786

Centisome position: 77.91

GC content: 51.42

Gene sequence:

>387_bases
ATGGGAAGAATTAACGTTCTTATCGTCGACGATGATGTCAATTTTTTATATGTTCTGGGTTTGCTCCTTGAGTCAAAAGA
TCTGGAGGTCACTTCAGCCTCGCGTGGAGCAGAAGCAGTCGACATCGTGCGGAAAACCACGTTCGACATGGTCATAACCG
ATTTCAGCATGCCGGGTATGAATGGTCTTGAACTGGCGGCTGCCGTAAAAGAACTGCATCCCGACCTCCCGGTCATGATG
GTTACGTGCGAAGTGACATCAGATCTCATTGAAATGGCTACAGATGTCGGCATCTGCCGGGTTTTGGACAAACCGGTCAA
TGTGGGACGGCTGTTGGCGGACATCCGGCACTTTTCCCGGAGGAAAGGCCGGCATACACCCGCCTGA

Upstream 100 bases:

>100_bases
CTGTGCGTAGTTGTATTTGATCGGGTAACTCTATCGTGTTGGCTGTTCATATGTTGTACTCTGACAGGCAGGCAAGGGAA
ATTGTCGCAGAGGTGCTCAT

Downstream 100 bases:

>100_bases
ACAGAGTGCGCATCGCGACCGTGTGCCGGATAGGTTCTCCCGAACAAACACGGGAAAGGGTGGTATCCTTTCCGGTAGTG
GTTCTGGCGAGACAGACTTA

Product: response regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 128; Mature: 127

Protein sequence:

>128_residues
MGRINVLIVDDDVNFLYVLGLLLESKDLEVTSASRGAEAVDIVRKTTFDMVITDFSMPGMNGLELAAAVKELHPDLPVMM
VTCEVTSDLIEMATDVGICRVLDKPVNVGRLLADIRHFSRRKGRHTPA

Sequences:

>Translated_128_residues
MGRINVLIVDDDVNFLYVLGLLLESKDLEVTSASRGAEAVDIVRKTTFDMVITDFSMPGMNGLELAAAVKELHPDLPVMM
VTCEVTSDLIEMATDVGICRVLDKPVNVGRLLADIRHFSRRKGRHTPA
>Mature_127_residues
GRINVLIVDDDVNFLYVLGLLLESKDLEVTSASRGAEAVDIVRKTTFDMVITDFSMPGMNGLELAAAVKELHPDLPVMMV
TCEVTSDLIEMATDVGICRVLDKPVNVGRLLADIRHFSRRKGRHTPA

Specific function: Member of the two-component regulatory system pilS/pilR that regulates the expression of type 4 fimbriae. When activated by pilS it acts in conjunction with sigma-54 as a transcriptional activator [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=128, Percent_Identity=32.8125, Blast_Score=67, Evalue=4e-13,
Organism=Escherichia coli, GI1790437, Length=112, Percent_Identity=31.25, Blast_Score=62, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 14083; Mature: 13951

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRINVLIVDDDVNFLYVLGLLLESKDLEVTSASRGAEAVDIVRKTTFDMVITDFSMPGM
CCCEEEEEEECCCCHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHEEECCCCCCC
NGLELAAAVKELHPDLPVMMVTCEVTSDLIEMATDVGICRVLDKPVNVGRLLADIRHFSR
CHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RKGRHTPA
HCCCCCCC
>Mature Secondary Structure 
GRINVLIVDDDVNFLYVLGLLLESKDLEVTSASRGAEAVDIVRKTTFDMVITDFSMPGM
CCEEEEEEECCCCHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHEEECCCCCCC
NGLELAAAVKELHPDLPVMMVTCEVTSDLIEMATDVGICRVLDKPVNVGRLLADIRHFSR
CHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
RKGRHTPA
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1317379; 8097014; 7911557; 10984043 [H]