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The map label for this gene is oprM [H]

Identifier: 39997789

GI number: 39997789

Start: 2972298

End: 2973836

Strand: Reverse

Name: oprM [H]

Synonym: GSU2695

Alternate gene names: 39997789

Gene position: 2973836-2972298 (Counterclockwise)

Preceding gene: 39997790

Following gene: 39997787

Centisome position: 77.97

GC content: 63.09

Gene sequence:

>1539_bases
ATGAGTATGTTCGGAATAACACAGAGTAGCAGAGCAACAGAGCAACAATCTGATCCCGCTGCCACCGTTTGTCGTGCAGC
CAGCAAGCGATCGCAGAGAGTTTCCGGTTTTGCGCCAAAGCCTTTCTCTGTTGCTCAAGTGCTCTATGTTTCAATTATGA
CATTGACCCTGGCCGGTTGCACCACCATGGCCCCCAAATACGAGCGGCCCGCCGCTCCGGTGCCAGTCGCGTGGCCCGAA
GGCCCCGCCTACAAACAGACGGCGTCCGCAACTGAAAAGCCGGTGGCCGACATCCCCTGGCAGGAGTTTTTCGTGGACGA
GAAGCTGCACAAGCTGATCGCTCTGGCTCTGGAGAACAACCGGGACCTGAGGGCCGCGGCTCTCAACATCGAGCGCTCCC
GAGCCCAGTACCAGATCCGGCGCTCGGATCTCTTCCCGAAGGTGGACGCCAGCGCCGGCGCAACGTTCACACGGCAGCCA
GAAGGCCTGTCGGCCACGGGCCGGGCCGATACCATCGATCAGTACAGTGTCGGACTCGGGGTCAGTTCCTACGAACTGGA
CCTTTTCGGCCGGGTACGCAGTCTCAAGGACCAGGCACTGGAACAGTACCTGGCCACTGAGCAGGCCCGGCGTAGCGTCC
AGATCAGCCTCGTGTCGGAGGTTGCCGTCAACTACCTCACCCTCGCCGCCGACCGCGAGCGGCTGAAACTGGCACAGGAA
ACCCTGGCGAACCAGCAGGAGTCCTACCAGCTCACCAAGAGCCGCTTCGATGCAGGCGTCTCTTCGGCTCTCGATCTGCA
TCAGGCCCAGACCAGCGTCGACGCCGCCCGGGTGGACATCGCCCGCTTCACCACCCTGGTGGCCCAGGATGGGAACGCCC
TGAGTCTGGTGGTCGGCTCGCCCGTGCCGGCGGAACTCCTTCCGTCGGCACTTTCCGACACCCTTACCGCCCTGAAGGAT
GTGGCGCCGGGCCTGCCGTCCGACGTGCTGTTGCGGCGCCCCGATATCCTCCAGGCCGAGAACCTTCTGAAGGGAGCCAA
TGCCAACATCGGTGCCGCCCGGGCCAACTTCTTCCCCCGCATAACTCTGGTGTCCAACGTTGGCTTTGGCAGCGATGATC
TGGCCAAGCTCTTCTCCGGCGATTCCTTCACCTGGAGCTTCGCACCCCGGATCACGCTCCCCATCTTCACTGCCGGAGCG
AACCAGGCCACGCTGGAGGTGGCGGAAGCGGACCGCAACATCGCCGTTGCCCAGTACGAGAAGGTGATTCAGACCGCCTT
CCGTGAGGTGGCCGATGCCCTGGCCCAGCGCGGTACCATCGACGACCAGCTTACGGCCCAGCAGTCCCTCTCCGATGCCA
CGGCCGAGAGCCATCGGTTGTCCCAGGCCCGCTACGAAAAGGGGGTGGATAACTATCTGCAGGTGCTCGATTCCCAACGG
GCCCTGTACAGCGCCCAGCAGAACCTGATCGGTGTCCGCCTCGTCCGGCTTCTCAATCTGGCGACCCTTTACAAGGTGCT
GGGCGGTGGGAGCGAGTAG

Upstream 100 bases:

>100_bases
ATTTTCATTCCGTTCTTTTTCGTGATGGTGTCGAAACTCTTTGCCAAAAAGCAGACTGCCGTACAGCCGCCCGCTGACAC
AGCGTCGGAGGTGAATTGAG

Downstream 100 bases:

>100_bases
CGGGCGGGGAACCTGAGGGCAATCACGGATACAGAGCGCAACTTCATCGAATGCTTGAAGTAGTCCACGAGGAATTGATC
TATGGACCGGAACAATTTTC

Product: outer membrane efflux protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MSMFGITQSSRATEQQSDPAATVCRAASKRSQRVSGFAPKPFSVAQVLYVSIMTLTLAGCTTMAPKYERPAAPVPVAWPE
GPAYKQTASATEKPVADIPWQEFFVDEKLHKLIALALENNRDLRAAALNIERSRAQYQIRRSDLFPKVDASAGATFTRQP
EGLSATGRADTIDQYSVGLGVSSYELDLFGRVRSLKDQALEQYLATEQARRSVQISLVSEVAVNYLTLAADRERLKLAQE
TLANQQESYQLTKSRFDAGVSSALDLHQAQTSVDAARVDIARFTTLVAQDGNALSLVVGSPVPAELLPSALSDTLTALKD
VAPGLPSDVLLRRPDILQAENLLKGANANIGAARANFFPRITLVSNVGFGSDDLAKLFSGDSFTWSFAPRITLPIFTAGA
NQATLEVAEADRNIAVAQYEKVIQTAFREVADALAQRGTIDDQLTAQQSLSDATAESHRLSQARYEKGVDNYLQVLDSQR
ALYSAQQNLIGVRLVRLLNLATLYKVLGGGSE

Sequences:

>Translated_512_residues
MSMFGITQSSRATEQQSDPAATVCRAASKRSQRVSGFAPKPFSVAQVLYVSIMTLTLAGCTTMAPKYERPAAPVPVAWPE
GPAYKQTASATEKPVADIPWQEFFVDEKLHKLIALALENNRDLRAAALNIERSRAQYQIRRSDLFPKVDASAGATFTRQP
EGLSATGRADTIDQYSVGLGVSSYELDLFGRVRSLKDQALEQYLATEQARRSVQISLVSEVAVNYLTLAADRERLKLAQE
TLANQQESYQLTKSRFDAGVSSALDLHQAQTSVDAARVDIARFTTLVAQDGNALSLVVGSPVPAELLPSALSDTLTALKD
VAPGLPSDVLLRRPDILQAENLLKGANANIGAARANFFPRITLVSNVGFGSDDLAKLFSGDSFTWSFAPRITLPIFTAGA
NQATLEVAEADRNIAVAQYEKVIQTAFREVADALAQRGTIDDQLTAQQSLSDATAESHRLSQARYEKGVDNYLQVLDSQR
ALYSAQQNLIGVRLVRLLNLATLYKVLGGGSE
>Mature_511_residues
SMFGITQSSRATEQQSDPAATVCRAASKRSQRVSGFAPKPFSVAQVLYVSIMTLTLAGCTTMAPKYERPAAPVPVAWPEG
PAYKQTASATEKPVADIPWQEFFVDEKLHKLIALALENNRDLRAAALNIERSRAQYQIRRSDLFPKVDASAGATFTRQPE
GLSATGRADTIDQYSVGLGVSSYELDLFGRVRSLKDQALEQYLATEQARRSVQISLVSEVAVNYLTLAADRERLKLAQET
LANQQESYQLTKSRFDAGVSSALDLHQAQTSVDAARVDIARFTTLVAQDGNALSLVVGSPVPAELLPSALSDTLTALKDV
APGLPSDVLLRRPDILQAENLLKGANANIGAARANFFPRITLVSNVGFGSDDLAKLFSGDSFTWSFAPRITLPIFTAGAN
QATLEVAEADRNIAVAQYEKVIQTAFREVADALAQRGTIDDQLTAQQSLSDATAESHRLSQARYEKGVDNYLQVLDSQRA
LYSAQQNLIGVRLVRLLNLATLYKVLGGGSE

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=470, Percent_Identity=40.2127659574468, Blast_Score=334, Evalue=7e-93,
Organism=Escherichia coli, GI2367348, Length=485, Percent_Identity=23.9175257731959, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 55459; Mature: 55328

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMFGITQSSRATEQQSDPAATVCRAASKRSQRVSGFAPKPFSVAQVLYVSIMTLTLAGC
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
TTMAPKYERPAAPVPVAWPEGPAYKQTASATEKPVADIPWQEFFVDEKLHKLIALALENN
HHCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
RDLRAAALNIERSRAQYQIRRSDLFPKVDASAGATFTRQPEGLSATGRADTIDQYSVGLG
CCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCC
VSSYELDLFGRVRSLKDQALEQYLATEQARRSVQISLVSEVAVNYLTLAADRERLKLAQE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
TLANQQESYQLTKSRFDAGVSSALDLHQAQTSVDAARVDIARFTTLVAQDGNALSLVVGS
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
PVPAELLPSALSDTLTALKDVAPGLPSDVLLRRPDILQAENLLKGANANIGAARANFFPR
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCHHHCCCCE
ITLVSNVGFGSDDLAKLFSGDSFTWSFAPRITLPIFTAGANQATLEVAEADRNIAVAQYE
EEEEECCCCCCHHHHHHHCCCCEEEEECCCEEEEEEECCCCCCEEEEECCCCCCHHHHHH
KVIQTAFREVADALAQRGTIDDQLTAQQSLSDATAESHRLSQARYEKGVDNYLQVLDSQR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
ALYSAQQNLIGVRLVRLLNLATLYKVLGGGSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SMFGITQSSRATEQQSDPAATVCRAASKRSQRVSGFAPKPFSVAQVLYVSIMTLTLAGC
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
TTMAPKYERPAAPVPVAWPEGPAYKQTASATEKPVADIPWQEFFVDEKLHKLIALALENN
HHCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
RDLRAAALNIERSRAQYQIRRSDLFPKVDASAGATFTRQPEGLSATGRADTIDQYSVGLG
CCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCC
VSSYELDLFGRVRSLKDQALEQYLATEQARRSVQISLVSEVAVNYLTLAADRERLKLAQE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
TLANQQESYQLTKSRFDAGVSSALDLHQAQTSVDAARVDIARFTTLVAQDGNALSLVVGS
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECC
PVPAELLPSALSDTLTALKDVAPGLPSDVLLRRPDILQAENLLKGANANIGAARANFFPR
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHHCCCCCCCCHHHCCCCE
ITLVSNVGFGSDDLAKLFSGDSFTWSFAPRITLPIFTAGANQATLEVAEADRNIAVAQYE
EEEEECCCCCCHHHHHHHCCCCEEEEECCCEEEEEEECCCCCCEEEEECCCCCCHHHHHH
KVIQTAFREVADALAQRGTIDDQLTAQQSLSDATAESHRLSQARYEKGVDNYLQVLDSQR
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
ALYSAQQNLIGVRLVRLLNLATLYKVLGGGSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]