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The map label for this gene is queA

Identifier: 39997714

GI number: 39997714

Start: 2890299

End: 2891330

Strand: Reverse

Name: queA

Synonym: GSU2620

Alternate gene names: 39997714

Gene position: 2891330-2890299 (Counterclockwise)

Preceding gene: 308513326

Following gene: 39997713

Centisome position: 75.81

GC content: 67.93

Gene sequence:

>1032_bases
ATGCTCGTTACTGAGTTCGACTACCATCTCCCCCCGGAACTTATCGCCCAGGAACCTCTTGTCCAGCGGGACGCCACCCG
GCTCATGACCGTGGAGCGGGACGGCGGCGGCATCGGGGAGATCCCGTTCCGCGGCATCGTGGACCTGTTCCGGCCCGGTG
ACCTGCTGGTGATCAACGATACGCGCGTCATCCCGGCGCGGCTCCTCGGCCGCAAGGAAAGCGGCGGCAAGACGGAGATC
TTTCTCGTGCGGCGCCGACCGGGGGACCACGAGACCTGGCACTGCCTGATCCGCTCTTCAAAACCGCCCCGCCCGGGCGT
GACGGTGCTCCTCCCGGAAGGGGTCCGCGCCGTTGTCCGCGAGCCCGGTGACGGGGAAACCTGGCTCGTGTCCTTCACAC
CCGGTGAAGGGTTTCAGGAGTGGCTGGATCGCAACGGCGCCATGCCCCTTCCTCCCTACATCAGGCGCGAGGCGGATGCG
GCGGACCGGGACCGGTACCAGACAGTGTTTGCCCGCAACCGCGGGGCGGTAGCTGCGCCCACTGCCGGCCTCCACATGAC
CGCCGGGCTCCTGGACGAGATCGCCGGCCGCGGGGTCCGGGTGGCGCCCGTGACGCTGCACGTGGGGCTCGGCACGTTCA
TGCCGATCCGGGTCGAACGGCTTGAAGATCATCGGATGCACCGGGAGCGGTACCACATCCCCCCCGCGACCGCCGACGCG
ATCAACGGGTGCAGGCGGGAGGGAGGCCGGGTCATCGCCCTGGGCACCACCGTCTGCCGGACCCTGGAGCAGGCAGCGGC
GGCCGACGGGACCATCGCAGCCGGCGAGGGCGAGGCCGACATCTTCATCTACCCCGGTTACCGCTTCAAGGCAGTGGACG
CCCTGATAACCAACTTCCACCTGCCCAAGTCGACCCTGCTCATGCTCGTGTCGGCTTTCGCCGGCAGGGACCTTCTCTTC
AGGGCCTACGGCGAGGCGGTGGCCAGGCGTTTCCGTTTCTTCAGCTACGGAGACGCGATGTTCATTTTCTGA

Upstream 100 bases:

>100_bases
GCCAAGCAGCGGGCCTCGGACGGATTCAGCTACCGTGAGATCCTGTCCTACTATTTCCCGGAAGCGGTGCTCACGCGCAT
GGACGATGGACAGGGGAGCG

Downstream 100 bases:

>100_bases
AAGGCGGGCGCGAGGCCGCGCAGGGGACGGACGGGATGATTTCCCGGCCCCGGGCTGCGGTGTCCGGCCCTGCGTGCAGG
CATCCTTGGCAGCTATCCAG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MLVTEFDYHLPPELIAQEPLVQRDATRLMTVERDGGGIGEIPFRGIVDLFRPGDLLVINDTRVIPARLLGRKESGGKTEI
FLVRRRPGDHETWHCLIRSSKPPRPGVTVLLPEGVRAVVREPGDGETWLVSFTPGEGFQEWLDRNGAMPLPPYIRREADA
ADRDRYQTVFARNRGAVAAPTAGLHMTAGLLDEIAGRGVRVAPVTLHVGLGTFMPIRVERLEDHRMHRERYHIPPATADA
INGCRREGGRVIALGTTVCRTLEQAAAADGTIAAGEGEADIFIYPGYRFKAVDALITNFHLPKSTLLMLVSAFAGRDLLF
RAYGEAVARRFRFFSYGDAMFIF

Sequences:

>Translated_343_residues
MLVTEFDYHLPPELIAQEPLVQRDATRLMTVERDGGGIGEIPFRGIVDLFRPGDLLVINDTRVIPARLLGRKESGGKTEI
FLVRRRPGDHETWHCLIRSSKPPRPGVTVLLPEGVRAVVREPGDGETWLVSFTPGEGFQEWLDRNGAMPLPPYIRREADA
ADRDRYQTVFARNRGAVAAPTAGLHMTAGLLDEIAGRGVRVAPVTLHVGLGTFMPIRVERLEDHRMHRERYHIPPATADA
INGCRREGGRVIALGTTVCRTLEQAAAADGTIAAGEGEADIFIYPGYRFKAVDALITNFHLPKSTLLMLVSAFAGRDLLF
RAYGEAVARRFRFFSYGDAMFIF
>Mature_343_residues
MLVTEFDYHLPPELIAQEPLVQRDATRLMTVERDGGGIGEIPFRGIVDLFRPGDLLVINDTRVIPARLLGRKESGGKTEI
FLVRRRPGDHETWHCLIRSSKPPRPGVTVLLPEGVRAVVREPGDGETWLVSFTPGEGFQEWLDRNGAMPLPPYIRREADA
ADRDRYQTVFARNRGAVAAPTAGLHMTAGLLDEIAGRGVRVAPVTLHVGLGTFMPIRVERLEDHRMHRERYHIPPATADA
INGCRREGGRVIALGTTVCRTLEQAAAADGTIAAGEGEADIFIYPGYRFKAVDALITNFHLPKSTLLMLVSAFAGRDLLF
RAYGEAVARRFRFFSYGDAMFIF

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=346, Percent_Identity=48.8439306358381, Blast_Score=308, Evalue=5e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_GEOSL (Q749X2)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_953665.1
- ProteinModelPortal:   Q749X2
- SMR:   Q749X2
- GeneID:   2686092
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU2620
- NMPDR:   fig|243231.1.peg.2603
- TIGR:   GSU2620
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- BioCyc:   GSUL243231:GSU_2620-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38040; Mature: 38040

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVTEFDYHLPPELIAQEPLVQRDATRLMTVERDGGGIGEIPFRGIVDLFRPGDLLVIND
CEEECCCCCCCHHHHHCCCHHHHCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCEEEEEC
TRVIPARLLGRKESGGKTEIFLVRRRPGDHETWHCLIRSSKPPRPGVTVLLPEGVRAVVR
CCCCHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCEEEECCCCHHHHHC
EPGDGETWLVSFTPGEGFQEWLDRNGAMPLPPYIRREADAADRDRYQTVFARNRGAVAAP
CCCCCCEEEEEECCCCHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCCCCEECC
TAGLHMTAGLLDEIAGRGVRVAPVTLHVGLGTFMPIRVERLEDHRMHRERYHIPPATADA
CCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCEEEEHHHHHHHHHHHHCCCCCCCHHH
INGCRREGGRVIALGTTVCRTLEQAAAADGTIAAGEGEADIFIYPGYRFKAVDALITNFH
HHHHHHCCCEEEEEHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCHHHHHHHHHHCC
LPKSTLLMLVSAFAGRDLLFRAYGEAVARRFRFFSYGDAMFIF
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEECCCEEEEC
>Mature Secondary Structure
MLVTEFDYHLPPELIAQEPLVQRDATRLMTVERDGGGIGEIPFRGIVDLFRPGDLLVIND
CEEECCCCCCCHHHHHCCCHHHHCCCEEEEEECCCCCCCCCCHHHHHHHCCCCCEEEEEC
TRVIPARLLGRKESGGKTEIFLVRRRPGDHETWHCLIRSSKPPRPGVTVLLPEGVRAVVR
CCCCHHHHHCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCCCEEEECCCCHHHHHC
EPGDGETWLVSFTPGEGFQEWLDRNGAMPLPPYIRREADAADRDRYQTVFARNRGAVAAP
CCCCCCEEEEEECCCCHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHCCCCCEECC
TAGLHMTAGLLDEIAGRGVRVAPVTLHVGLGTFMPIRVERLEDHRMHRERYHIPPATADA
CCCHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCEEEEHHHHHHHHHHHHCCCCCCCHHH
INGCRREGGRVIALGTTVCRTLEQAAAADGTIAAGEGEADIFIYPGYRFKAVDALITNFH
HHHHHHCCCEEEEEHHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCHHHHHHHHHHCC
LPKSTLLMLVSAFAGRDLLFRAYGEAVARRFRFFSYGDAMFIF
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA