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The map label for this gene is tgt-2 [H]

Identifier: 39997713

GI number: 39997713

Start: 2889104

End: 2890225

Strand: Reverse

Name: tgt-2 [H]

Synonym: GSU2619

Alternate gene names: 39997713

Gene position: 2890225-2889104 (Counterclockwise)

Preceding gene: 39997714

Following gene: 39997712

Centisome position: 75.78

GC content: 64.71

Gene sequence:

>1122_bases
GTGCAGGCATCCTTGGCAGCTATCCAGTTCCGATTACTGAAGCAGGACGGCGCGGCCCGTCTCGGCTCTTTGACAACGCC
CCACGGGCTCATCGAGACCCCGATCTTCATGCCGGTGGGAACCCAGGCCACGGTCAAGGCCATGACCCCCGAGGAGCTCG
ATGCCATCGGTGCGCGGATCATCCTTGCCAACACCTATCACCTCTATATCCGCCCGGGCCATGAACTGATCCGGCGGATG
GGGGGGCTTCACCGCTTCATGCACTGGGACCGGCCGATCCTCACCGACAGCGGCGGGTTCCAGGTGTTCAGCCTTAACGA
GCTCAGAAAGATTTCGGAGGAGGGGGTGCGGTTCCAGTCCCACCTGGACGGGTCGTACCACACCATCCGGCCCGAGGACG
CCGTCGCCATTCAGGAGGCCCTGGGCAGTGACATCGCCATGTGCTTCGACGAGTGCACACCCTATCCTGCGACGCACGAC
TATGCCCGGCGCTCCATGGAGCTGACCACCCGCTGGGCCCGCCGCTGCAAGGATGCCCGCCGCCGCGGGGATCAGGCCCT
GTTCGGCATCGTCCAGGGGGGCATGTACCGCGACCTGCGCGAGCAGAGCGCCGCAGAGCTGGTGGAAATCGGATTCGACG
GGTACGCCGTGGGCGGGCTTTCAGTGGGAGAGGAGAAGGAGCATATGTACGGCGTCATGGAATGGACGGCGCCGCTGCTC
CCTGTGGACCAGCCCCGTTACGTGATGGGGATCGGCACCCCGGAGGATCTGGTGGAGGCGGTCTGGCGCGGCTTCGACAT
GTTCGACTGCGTGATGCCCACCCGCAACGCACGCAACGGAATGCTCTTCACCTCCTTCGGCCGTCTGAACATCAAGGGGG
CTGCCTACGCGGAGGATCAGGGGCCAGTGGACCCCGCATGCGGCTGTTACGTCTGCCGGAACTACAGCAGGGCCTACCTG
AGGCACCTCTACCGTTCCGGCGAGATCCTGGCGTCGCGCCTCAATACGTACCACAACCTCGCCTACTACCTGGATCTGAT
GGCGCAGATCCGGACGGCCATCGCCGAGGAGAGGTTCGACTCCTTCCGCCGGGAGTTTTACGCCCGCCGGGAAACGCAGT
AA

Upstream 100 bases:

>100_bases
TACGGAGACGCGATGTTCATTTTCTGAAAGGCGGGCGCGAGGCCGCGCAGGGGACGGACGGGATGATTTCCCGGCCCCGG
GCTGCGGTGTCCGGCCCTGC

Downstream 100 bases:

>100_bases
CGGTAGCAGTTTTTTCACAGTCGTTTAACAGAGGGCTCTGCCCTACACAATACACGTGGAGGTACACACAATGCTCGGAA
TCGCGTTTGCAATGGCGGCA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MQASLAAIQFRLLKQDGAARLGSLTTPHGLIETPIFMPVGTQATVKAMTPEELDAIGARIILANTYHLYIRPGHELIRRM
GGLHRFMHWDRPILTDSGGFQVFSLNELRKISEEGVRFQSHLDGSYHTIRPEDAVAIQEALGSDIAMCFDECTPYPATHD
YARRSMELTTRWARRCKDARRRGDQALFGIVQGGMYRDLREQSAAELVEIGFDGYAVGGLSVGEEKEHMYGVMEWTAPLL
PVDQPRYVMGIGTPEDLVEAVWRGFDMFDCVMPTRNARNGMLFTSFGRLNIKGAAYAEDQGPVDPACGCYVCRNYSRAYL
RHLYRSGEILASRLNTYHNLAYYLDLMAQIRTAIAEERFDSFRREFYARRETQ

Sequences:

>Translated_373_residues
MQASLAAIQFRLLKQDGAARLGSLTTPHGLIETPIFMPVGTQATVKAMTPEELDAIGARIILANTYHLYIRPGHELIRRM
GGLHRFMHWDRPILTDSGGFQVFSLNELRKISEEGVRFQSHLDGSYHTIRPEDAVAIQEALGSDIAMCFDECTPYPATHD
YARRSMELTTRWARRCKDARRRGDQALFGIVQGGMYRDLREQSAAELVEIGFDGYAVGGLSVGEEKEHMYGVMEWTAPLL
PVDQPRYVMGIGTPEDLVEAVWRGFDMFDCVMPTRNARNGMLFTSFGRLNIKGAAYAEDQGPVDPACGCYVCRNYSRAYL
RHLYRSGEILASRLNTYHNLAYYLDLMAQIRTAIAEERFDSFRREFYARRETQ
>Mature_373_residues
MQASLAAIQFRLLKQDGAARLGSLTTPHGLIETPIFMPVGTQATVKAMTPEELDAIGARIILANTYHLYIRPGHELIRRM
GGLHRFMHWDRPILTDSGGFQVFSLNELRKISEEGVRFQSHLDGSYHTIRPEDAVAIQEALGSDIAMCFDECTPYPATHD
YARRSMELTTRWARRCKDARRRGDQALFGIVQGGMYRDLREQSAAELVEIGFDGYAVGGLSVGEEKEHMYGVMEWTAPLL
PVDQPRYVMGIGTPEDLVEAVWRGFDMFDCVMPTRNARNGMLFTSFGRLNIKGAAYAEDQGPVDPACGCYVCRNYSRAYL
RHLYRSGEILASRLNTYHNLAYYLDLMAQIRTAIAEERFDSFRREFYARRETQ

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=349, Percent_Identity=47.8510028653295, Blast_Score=333, Evalue=2e-91,
Organism=Escherichia coli, GI1786607, Length=368, Percent_Identity=58.4239130434783, Blast_Score=444, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI17544680, Length=360, Percent_Identity=44.7222222222222, Blast_Score=321, Evalue=3e-88,
Organism=Caenorhabditis elegans, GI25146502, Length=176, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19920510, Length=352, Percent_Identity=45.7386363636364, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI21355311, Length=214, Percent_Identity=26.6355140186916, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42319; Mature: 42319

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQASLAAIQFRLLKQDGAARLGSLTTPHGLIETPIFMPVGTQATVKAMTPEELDAIGARI
CCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCEEEECCCCCEEECCCHHHHHHHCCEE
ILANTYHLYIRPGHELIRRMGGLHRFMHWDRPILTDSGGFQVFSLNELRKISEEGVRFQS
EEEEEEEEEEECCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEHHHHHHHHHHCCCHHH
HLDGSYHTIRPEDAVAIQEALGSDIAMCFDECTPYPATHDYARRSMELTTRWARRCKDAR
HCCCCEEEECCHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RRGDQALFGIVQGGMYRDLREQSAAELVEIGFDGYAVGGLSVGEEKEHMYGVMEWTAPLL
HCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHCCCCC
PVDQPRYVMGIGTPEDLVEAVWRGFDMFDCVMPTRNARNGMLFTSFGRLNIKGAAYAEDQ
CCCCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEECCCEEECCCEECCCC
GPVDPACGCYVCRNYSRAYLRHLYRSGEILASRLNTYHNLAYYLDLMAQIRTAIAEERFD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFRREFYARRETQ
HHHHHHHHHCCCC
>Mature Secondary Structure
MQASLAAIQFRLLKQDGAARLGSLTTPHGLIETPIFMPVGTQATVKAMTPEELDAIGARI
CCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCEEEECCCCCEEECCCHHHHHHHCCEE
ILANTYHLYIRPGHELIRRMGGLHRFMHWDRPILTDSGGFQVFSLNELRKISEEGVRFQS
EEEEEEEEEEECCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEHHHHHHHHHHCCCHHH
HLDGSYHTIRPEDAVAIQEALGSDIAMCFDECTPYPATHDYARRSMELTTRWARRCKDAR
HCCCCEEEECCHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RRGDQALFGIVQGGMYRDLREQSAAELVEIGFDGYAVGGLSVGEEKEHMYGVMEWTAPLL
HCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHCCCCC
PVDQPRYVMGIGTPEDLVEAVWRGFDMFDCVMPTRNARNGMLFTSFGRLNIKGAAYAEDQ
CCCCCCEEEECCCHHHHHHHHHCCCCHHHHHCCCCCCCCCEEEEECCCEEECCCEECCCC
GPVDPACGCYVCRNYSRAYLRHLYRSGEILASRLNTYHNLAYYLDLMAQIRTAIAEERFD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SFRREFYARRETQ
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]