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The map label for this gene is trpA

Identifier: 39997467

GI number: 39997467

Start: 2598080

End: 2598874

Strand: Reverse

Name: trpA

Synonym: GSU2371

Alternate gene names: 39997467

Gene position: 2598874-2598080 (Counterclockwise)

Preceding gene: 39997468

Following gene: 39997466

Centisome position: 68.14

GC content: 65.66

Gene sequence:

>795_bases
ATGAGCAGAATTGCCGGCACATTTGCCGAATTGAAGCACAACAGCCGCAAGGCCCTCGTTACCTTCATCACCGCCGGTGA
CCCGGACCTGGCCGCCACGGAAGCGCTGATTCCGCTTCTGGCTGAAAGCGGCGCCGACATCGTGGAACTTGGCGTTCCCT
TTTCGGATCCAATGGCCGACGGCCCCACCATCCAGCTTTCTTCCGAGCGGGCCCTTGCTGCGGGGACGACCCTGCCGAAG
ATCCTCGACATGGTGCGCCGGGTCCGTACCCGCTGCCAGGTGCCCATCGTGCTCATGGGCTACTACAATCCGATTCTGAT
CCACGGCCTTGAGCGCTTTGCGGCGGATGCCTCGGCCGCGGGGGTCGACGGCGTGCTCCTGGTTGACCTTCCCCCGGAGG
AGGCGGCCGAGTTCAAGGCATGTGCCGACCGCCACGGGCTCGATGTTATCTTCCTTCTCACCCCCACCTCCGACGAAGGG
CGCATCCGGAAAGTGGCGCGGCAGGCCCGCGGGTTCGTCTACTATGTGTCGGTGACCGGCGTGACCGGCGCGCGGAGCGG
CGTGGAGGCGTCCGTTTCCTCCAACGTGGCCGCCATTCGCGAGGCGATCACCGTGCCGGTCGTGGTCGGCTTCGGCATCT
CGACGCCTGACCAGGCTGCCCAGGTGGCCGCCTCGGCCGACGGCGTGGTCGTGGGGAGCGCCATTGTAAAATTGTTCGAG
CGTTTCACGGCCGCCGAGCTCGGGCGTGAGGTCGCAACCTTCGTTTCCGCGCTGCGCGAAGCGATCGGAAATTGA

Upstream 100 bases:

>100_bases
GAGCAGTTTCAAGGTGGAGAATCGGTAACCCGGGACCGGGGACCGGGGATCGGCACGATACCTGGAAACCCGGTCCGGCA
ACCATTCCGAAAGGTTTCCC

Downstream 100 bases:

>100_bases
CTTTAGCTGACTTTTTTGGTAATAAAACTTTTCAATTTTACTAAAAAATCCGGGATGCGAACGTCCCGACGGAGTGACGA
CATGGCGTGGTTCAAACGCG

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MSRIAGTFAELKHNSRKALVTFITAGDPDLAATEALIPLLAESGADIVELGVPFSDPMADGPTIQLSSERALAAGTTLPK
ILDMVRRVRTRCQVPIVLMGYYNPILIHGLERFAADASAAGVDGVLLVDLPPEEAAEFKACADRHGLDVIFLLTPTSDEG
RIRKVARQARGFVYYVSVTGVTGARSGVEASVSSNVAAIREAITVPVVVGFGISTPDQAAQVAASADGVVVGSAIVKLFE
RFTAAELGREVATFVSALREAIGN

Sequences:

>Translated_264_residues
MSRIAGTFAELKHNSRKALVTFITAGDPDLAATEALIPLLAESGADIVELGVPFSDPMADGPTIQLSSERALAAGTTLPK
ILDMVRRVRTRCQVPIVLMGYYNPILIHGLERFAADASAAGVDGVLLVDLPPEEAAEFKACADRHGLDVIFLLTPTSDEG
RIRKVARQARGFVYYVSVTGVTGARSGVEASVSSNVAAIREAITVPVVVGFGISTPDQAAQVAASADGVVVGSAIVKLFE
RFTAAELGREVATFVSALREAIGN
>Mature_263_residues
SRIAGTFAELKHNSRKALVTFITAGDPDLAATEALIPLLAESGADIVELGVPFSDPMADGPTIQLSSERALAAGTTLPKI
LDMVRRVRTRCQVPIVLMGYYNPILIHGLERFAADASAAGVDGVLLVDLPPEEAAEFKACADRHGLDVIFLLTPTSDEGR
IRKVARQARGFVYYVSVTGVTGARSGVEASVSSNVAAIREAITVPVVVGFGISTPDQAAQVAASADGVVVGSAIVKLFER
FTAAELGREVATFVSALREAIGN

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=243, Percent_Identity=40.3292181069959, Blast_Score=180, Evalue=9e-47,
Organism=Saccharomyces cerevisiae, GI6321412, Length=234, Percent_Identity=47.008547008547, Blast_Score=209, Evalue=3e-55,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_GEOSL (Q74AI3)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_953418.1
- ProteinModelPortal:   Q74AI3
- SMR:   Q74AI3
- GeneID:   2686600
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU2371
- NMPDR:   fig|243231.1.peg.2356
- TIGR:   GSU2371
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- BioCyc:   GSUL243231:GSU_2371-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 27633; Mature: 27502

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIAGTFAELKHNSRKALVTFITAGDPDLAATEALIPLLAESGADIVELGVPFSDPMAD
CCCHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
GPTIQLSSERALAAGTTLPKILDMVRRVRTRCQVPIVLMGYYNPILIHGLERFAADASAA
CCEEEECCCCHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCC
GVDGVLLVDLPPEEAAEFKACADRHGLDVIFLLTPTSDEGRIRKVARQARGFVYYVSVTG
CCCCEEEEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCEEEEEEEEC
VTGARSGVEASVSSNVAAIREAITVPVVVGFGISTPDQAAQVAASADGVVVGSAIVKLFE
CCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHCCCCEEHHHHHHHHHH
RFTAAELGREVATFVSALREAIGN
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRIAGTFAELKHNSRKALVTFITAGDPDLAATEALIPLLAESGADIVELGVPFSDPMAD
CCHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
GPTIQLSSERALAAGTTLPKILDMVRRVRTRCQVPIVLMGYYNPILIHGLERFAADASAA
CCEEEECCCCHHHCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCCCCC
GVDGVLLVDLPPEEAAEFKACADRHGLDVIFLLTPTSDEGRIRKVARQARGFVYYVSVTG
CCCCEEEEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHCCEEEEEEEEC
VTGARSGVEASVSSNVAAIREAITVPVVVGFGISTPDQAAQVAASADGVVVGSAIVKLFE
CCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHCCCCEEHHHHHHHHHH
RFTAAELGREVATFVSALREAIGN
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA