Click here to switch to the map view.

The map label for this gene is birA [H]

Identifier: 39997033

GI number: 39997033

Start: 2116743

End: 2117714

Strand: Reverse

Name: birA [H]

Synonym: GSU1935

Alternate gene names: 39997033

Gene position: 2117714-2116743 (Counterclockwise)

Preceding gene: 39997034

Following gene: 39997032

Centisome position: 55.52

GC content: 62.35

Gene sequence:

>972_bases
ATGGATCGGAAGATCCTGGAGCTGTTCCGGAGCCGTTCGGGGGGGGTCGTCTCCGGTGAGGCCCTGAGCACTCTGCTGGG
TGTTTCGCGAACCGCCGTCTGGAAGCACATCAAAAACCTCAAAAATCTGGGCTACCGCATCGAGTCGGTCCCCTCCCGCG
GTTACCGTCTCGTGGCCTCTCCTGACATCCTCATCCCGGCCGAGGTCTCCGCAGGGATCTCGGTGCGCCGGATCGGCACC
CATGTGGTCTGTTTCCGGGAAACGGTGTCTACCAATACCGTGGCATCGCACCTGGCTGCCGAAGGCGCTGCCGAGGGGAC
CGTGGTGCTGGCTGACGCCCAGACCGGCGGCAAGGGCCGACTGGGACGGCGCTGGGAATCACCGCCGGGGGTAAATGTAT
ACTGCTCGATAATCCTCCGTCCTCCCATATCGCCGCTTCATTCACCTCAACTCACCCTCCTGTCGGCCGTGGCAACGGCC
GAGGCCATCGAGCGCACTTCGGGGCTTGTTCCCGCCATCAAGTGGCCCAACGACATCCTGGTGAACGGCTTCAAGGTGGC
GGGAATGCTCAATGAGTTGAGCGCCGAAACGGAGCGGGTTGAGTACCTCGTCCTGGGGATCGGTGTCAACATCAACATGG
AGCGGGACCAGTTTCCCGGCGATCTTCGCTATCCGGCCAGCTCACTGGCCCTGGAGGCGGGGCGGACCGTGGAGCGCATT
CCGTTTGTCAGGGCGCTGCTGGAATCACTGGATCGCCTGTACGATGAGTATTTACTGAAGGGGTTCTCTCCCGTGCGCGA
TGCCTGGCTCGCCCGTAGCGCCGTCATGGGCCGGCGGGTGAAGGTCGATGGTGGTGCTGGTATCCTCGCCGGTACGGTTG
AAGGGATAGATGAAATCGGAGCCTTGTTGGTAAGAACCGCCGGAGGTTCTCTGGAGCGGGTTCTGGCAGGTGACGTTTCG
CTCGAAGGTTGA

Upstream 100 bases:

>100_bases
GAGCATTGACCCACTCGGCGCGTGCCATGGACATCTCCATGCTGTTGGAGGCCGAGTGAGCGGAGAGCGCCCACGCCCTG
CCGAGGCGACCGAGCGGGAC

Downstream 100 bases:

>100_bases
GGGGACGGCGGTAACGCGCCGTATTCCCGGGGGACGTCTCACCGTGTGGAGGAAGTGTGCTTCTTGTTATAGACGTGGGT
AATACCAATATCGTGCTCGG

Product: birA biofunctional protein

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MDRKILELFRSRSGGVVSGEALSTLLGVSRTAVWKHIKNLKNLGYRIESVPSRGYRLVASPDILIPAEVSAGISVRRIGT
HVVCFRETVSTNTVASHLAAEGAAEGTVVLADAQTGGKGRLGRRWESPPGVNVYCSIILRPPISPLHSPQLTLLSAVATA
EAIERTSGLVPAIKWPNDILVNGFKVAGMLNELSAETERVEYLVLGIGVNINMERDQFPGDLRYPASSLALEAGRTVERI
PFVRALLESLDRLYDEYLLKGFSPVRDAWLARSAVMGRRVKVDGGAGILAGTVEGIDEIGALLVRTAGGSLERVLAGDVS
LEG

Sequences:

>Translated_323_residues
MDRKILELFRSRSGGVVSGEALSTLLGVSRTAVWKHIKNLKNLGYRIESVPSRGYRLVASPDILIPAEVSAGISVRRIGT
HVVCFRETVSTNTVASHLAAEGAAEGTVVLADAQTGGKGRLGRRWESPPGVNVYCSIILRPPISPLHSPQLTLLSAVATA
EAIERTSGLVPAIKWPNDILVNGFKVAGMLNELSAETERVEYLVLGIGVNINMERDQFPGDLRYPASSLALEAGRTVERI
PFVRALLESLDRLYDEYLLKGFSPVRDAWLARSAVMGRRVKVDGGAGILAGTVEGIDEIGALLVRTAGGSLERVLAGDVS
LEG
>Mature_323_residues
MDRKILELFRSRSGGVVSGEALSTLLGVSRTAVWKHIKNLKNLGYRIESVPSRGYRLVASPDILIPAEVSAGISVRRIGT
HVVCFRETVSTNTVASHLAAEGAAEGTVVLADAQTGGKGRLGRRWESPPGVNVYCSIILRPPISPLHSPQLTLLSAVATA
EAIERTSGLVPAIKWPNDILVNGFKVAGMLNELSAETERVEYLVLGIGVNINMERDQFPGDLRYPASSLALEAGRTVERI
PFVRALLESLDRLYDEYLLKGFSPVRDAWLARSAVMGRRVKVDGGAGILAGTVEGIDEIGALLVRTAGGSLERVLAGDVS
LEG

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=309, Percent_Identity=29.4498381877023, Blast_Score=122, Evalue=4e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 34572; Mature: 34572

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRKILELFRSRSGGVVSGEALSTLLGVSRTAVWKHIKNLKNLGYRIESVPSRGYRLVAS
CCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEC
PDILIPAEVSAGISVRRIGTHVVCFRETVSTNTVASHLAAEGAAEGTVVLADAQTGGKGR
CCEEEECCCCCCEEEEECCCEEEEEEHHHCHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
LGRRWESPPGVNVYCSIILRPPISPLHSPQLTLLSAVATAEAIERTSGLVPAIKWPNDIL
CCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCHHH
VNGFKVAGMLNELSAETERVEYLVLGIGVNINMERDQFPGDLRYPASSLALEAGRTVERI
CCCCHHHHHHHHHHHHHHHEEEEEEEECEEEEECCCCCCCCCCCCHHHHHHHCCCCHHHH
PFVRALLESLDRLYDEYLLKGFSPVRDAWLARSAVMGRRVKVDGGAGILAGTVEGIDEIG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEECCCCEEEEHHHHHHHHHH
ALLVRTAGGSLERVLAGDVSLEG
HHHHHCCCCCHHHHHCCCCCCCC
>Mature Secondary Structure
MDRKILELFRSRSGGVVSGEALSTLLGVSRTAVWKHIKNLKNLGYRIESVPSRGYRLVAS
CCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEC
PDILIPAEVSAGISVRRIGTHVVCFRETVSTNTVASHLAAEGAAEGTVVLADAQTGGKGR
CCEEEECCCCCCEEEEECCCEEEEEEHHHCHHHHHHHHHHCCCCCCEEEEEECCCCCCCC
LGRRWESPPGVNVYCSIILRPPISPLHSPQLTLLSAVATAEAIERTSGLVPAIKWPNDIL
CCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCHHH
VNGFKVAGMLNELSAETERVEYLVLGIGVNINMERDQFPGDLRYPASSLALEAGRTVERI
CCCCHHHHHHHHHHHHHHHEEEEEEEECEEEEECCCCCCCCCCCCHHHHHHHCCCCHHHH
PFVRALLESLDRLYDEYLLKGFSPVRDAWLARSAVMGRRVKVDGGAGILAGTVEGIDEIG
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEECCCCEEEEHHHHHHHHHH
ALLVRTAGGSLERVLAGDVSLEG
HHHHHCCCCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]