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The map label for this gene is panB

Identifier: 39996805

GI number: 39996805

Start: 1868888

End: 1869691

Strand: Direct

Name: panB

Synonym: GSU1705

Alternate gene names: 39996805

Gene position: 1868888-1869691 (Clockwise)

Preceding gene: 39996804

Following gene: 39996806

Centisome position: 49.0

GC content: 59.33

Gene sequence:

>804_bases
GTGAACCGCGCGAAAACCATCCTCGATATCCAGAAAATGAAGGCGACGGGCGAGAAGATAACCGTCCTTACCAGCTACGA
CTATCCCTTTACCCGCATTATGGATGAATGCGGCATCGACATGATTCTGGTCGGAGACTCGGTCGGTGTTGTCTTTGCCG
GTCACGACAACACCCTGCCGGTTACCGTGGACGATATGCTCTATCACACCCGCGCCGTTACCCGGGCCAGACCAAAGGCT
CTGGTGGTTACGGACATGCCGTTTCTTTCCTACCAGACCGATTTGCGCGATGCCCGCATCAACGCCGGACGCCTGGTCAA
GGAGGGTGGAGCCGAAGCGGTGAAGCTGGAGGGGGGAGCCCACGTGACGGACACCATCAGGGCCATCGTCGATATGGACA
TCCCGGTCATGGCCCACATCGGACTTACCCCCCAATCGATCCACCGGATGGGGGGCTACAAGGTGCAGGGCAAAAAGGAC
GAGCAGGCTCAGCGGTTGCTGGAGGATGCCCTCGCGGTCCAGGAGGCGGGAGCCTTTGCCGTGGTCCTGGAAGGGATTCC
GCTCAAGCTTGCCGGCCGCATCACCGCAGAACTCTCCATTCCCACCATTGGCATCGGTGCCGGTCCCCACTGCGACGGCC
AGGTTCTCGTTATCCACGACATCCTCGGCCTGTGCGAGAAGTACTCTCCCAAATTCGTCAAGCGCTACGGCGATGCTCGT
ACGCTCATTTCCGACGCGGTAGCGTCCTACATCTCAGAAGTCAAGAAAGGAGAATTCCCCACCGAGGGGCATTCGTTCAG
CTGA

Upstream 100 bases:

>100_bases
CCGCACCGACGAGGATAGGATGAGCTTTCCCTTCCATGGTCGTTTTCGTGCGCCTCCGGACCCCGGTGGCGCATTTGTTA
TTTGACAGGGGACAACCACG

Downstream 100 bases:

>100_bases
CATGCGCATCATAGATTCTGTTGCCGACATGCAGGCATTTTCCCGCGATGCCAGGCGTTCGGGGAAAACCATTGCCTTGG
TCCCCACCATGGGCTATCTC

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MNRAKTILDIQKMKATGEKITVLTSYDYPFTRIMDECGIDMILVGDSVGVVFAGHDNTLPVTVDDMLYHTRAVTRARPKA
LVVTDMPFLSYQTDLRDARINAGRLVKEGGAEAVKLEGGAHVTDTIRAIVDMDIPVMAHIGLTPQSIHRMGGYKVQGKKD
EQAQRLLEDALAVQEAGAFAVVLEGIPLKLAGRITAELSIPTIGIGAGPHCDGQVLVIHDILGLCEKYSPKFVKRYGDAR
TLISDAVASYISEVKKGEFPTEGHSFS

Sequences:

>Translated_267_residues
MNRAKTILDIQKMKATGEKITVLTSYDYPFTRIMDECGIDMILVGDSVGVVFAGHDNTLPVTVDDMLYHTRAVTRARPKA
LVVTDMPFLSYQTDLRDARINAGRLVKEGGAEAVKLEGGAHVTDTIRAIVDMDIPVMAHIGLTPQSIHRMGGYKVQGKKD
EQAQRLLEDALAVQEAGAFAVVLEGIPLKLAGRITAELSIPTIGIGAGPHCDGQVLVIHDILGLCEKYSPKFVKRYGDAR
TLISDAVASYISEVKKGEFPTEGHSFS
>Mature_267_residues
MNRAKTILDIQKMKATGEKITVLTSYDYPFTRIMDECGIDMILVGDSVGVVFAGHDNTLPVTVDDMLYHTRAVTRARPKA
LVVTDMPFLSYQTDLRDARINAGRLVKEGGAEAVKLEGGAHVTDTIRAIVDMDIPVMAHIGLTPQSIHRMGGYKVQGKKD
EQAQRLLEDALAVQEAGAFAVVLEGIPLKLAGRITAELSIPTIGIGAGPHCDGQVLVIHDILGLCEKYSPKFVKRYGDAR
TLISDAVASYISEVKKGEFPTEGHSFS

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=264, Percent_Identity=45.4545454545455, Blast_Score=237, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6319653, Length=272, Percent_Identity=38.6029411764706, Blast_Score=199, Evalue=4e-52,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_GEOSL (Q74CG8)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952756.1
- ProteinModelPortal:   Q74CG8
- SMR:   Q74CG8
- GeneID:   2685413
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1705
- NMPDR:   fig|243231.1.peg.1694
- TIGR:   GSU1705
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BioCyc:   GSUL243231:GSU_1705-MONOMER
- BRENDA:   2.1.2.11
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28950; Mature: 28950

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: ACT_SITE 184-184 BINDING 85-85 BINDING 115-115

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRAKTILDIQKMKATGEKITVLTSYDYPFTRIMDECGIDMILVGDSVGVVFAGHDNTLP
CCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCEEEEECCCEEEEEECCCCCCC
VTVDDMLYHTRAVTRARPKALVVTDMPFLSYQTDLRDARINAGRLVKEGGAEAVKLEGGA
EEHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCHHHCCCCCCCEECCCCCCEEEECCCC
HVTDTIRAIVDMDIPVMAHIGLTPQSIHRMGGYKVQGKKDEQAQRLLEDALAVQEAGAFA
CHHHHHHHHHCCCCCEEEECCCCHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCCEE
VVLEGIPLKLAGRITAELSIPTIGIGAGPHCDGQVLVIHDILGLCEKYSPKFVKRYGDAR
EEEECCCEEEECEEEEEEECCEEECCCCCCCCCCEEEHHHHHHHHHHCCHHHHHHHCCHH
TLISDAVASYISEVKKGEFPTEGHSFS
HHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNRAKTILDIQKMKATGEKITVLTSYDYPFTRIMDECGIDMILVGDSVGVVFAGHDNTLP
CCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCEEEEECCCEEEEEECCCCCCC
VTVDDMLYHTRAVTRARPKALVVTDMPFLSYQTDLRDARINAGRLVKEGGAEAVKLEGGA
EEHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCCHHHCCCCCCCEECCCCCCEEEECCCC
HVTDTIRAIVDMDIPVMAHIGLTPQSIHRMGGYKVQGKKDEQAQRLLEDALAVQEAGAFA
CHHHHHHHHHCCCCCEEEECCCCHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCCEE
VVLEGIPLKLAGRITAELSIPTIGIGAGPHCDGQVLVIHDILGLCEKYSPKFVKRYGDAR
EEEECCCEEEECEEEEEEECCEEECCCCCCCCCCEEEHHHHHHHHHHCCHHHHHHHCCHH
TLISDAVASYISEVKKGEFPTEGHSFS
HHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA