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The map label for this gene is emrB [H]

Identifier: 39996580

GI number: 39996580

Start: 1619796

End: 1621349

Strand: Reverse

Name: emrB [H]

Synonym: GSU1480

Alternate gene names: 39996580

Gene position: 1621349-1619796 (Counterclockwise)

Preceding gene: 39996581

Following gene: 39996552

Centisome position: 42.51

GC content: 59.97

Gene sequence:

>1554_bases
GTGGAACCGGACACGAAAACCGTCAACAAGTGGCTCATCACCATCACGGTGATGCTGCCGACAATCATGGAGATCGTCGA
CACCTCCGTGGCGAACGTGGCGCTTCCCCACATGCAGGGGAGCCTCAACGCCGGCACCGACGAAATCACCTGGGTGCTCA
CCTCCTACCTGGTGAGCAACGCCGTGGTCCTCCCCATGACCGGCTGGTTTTCGCGACTCTTCGGCAGGAAACGGTTCCTC
ATCTCCTGCATCACCCTCTTCACCATTGCCTCGTTTCTCTGCGGCGCCGCGCCCAACCTGACAAGCCTCATCTTCTTCAG
GATAGTCCAGGGCGCTGCTGGTGGAGCCCTGATCCCGAGCAGCCAGGCGATCCTCATGGAGACATTCCCGCCCCGGGAAC
GGGGAATGGCCAACGCCATCTTCGGCATCGGCGCCATGTTCGGCCCCATCATCGGCCCGGCCCTGGGGGGCTGGATCACC
GACAACCTGAACTGGCGCTGGATTTTCTACATCAATATCCCCATAGGCATCCTGGCGGTGATCATGGCGGTCTACTTCAT
CTTCGACCCCCCCTATCTCCGCCGCTCAAAGCTGTCCATCGACTGGTGGGGGCTGGGGTTGCTGACCGTCGGACTCGGGG
CGCTGCAGATCGTGCTCGACAAGGGACAGCAGGACGACTGGTTCAACTCATCCTTTATCACCGGCGCATCCATCATCTCG
GTACTTGCGCTGATGCTTCTCATCTATGTGGAGCTCAGGCACGAGCACCCCATCGTCAACCTGCGCCTGTTCAAGGACAT
TTCCTTTTCCGCAGGCAATCTGATCATGTTCATGATCGGGTTCTGCCTCTACAGCTCGATCATGCTGATCCCCCTCTTCC
TCCAGACGCTCATGGGCTACGATGCAACCCTGGCGGGAATGGTCCTGGCCCCAGGGGGGGTGGCCACCCTGCTGACCATG
CCGTTCGTGGGGGCGATCATCTCACGCTACGATGGCCGAAAAGTGGTCTTGGGAGGGCTTCTGATTGGAGCGACCGCCAT
GTTCCTCATGCAACGGTTCACCCTGGAGGCATCCTACTGGGACTTCGTTTGGCCGCGCGTGGTCCTCGGCGTGGGGCTCG
CCATGCTGTTCGTTCCCCTCAATACCGTGACCCTCATGCCGATTCCCAAGGAAGAGATGGGGAACGCCACCGGCATGCTC
AACCTGATGCGCAATATCGGCGGCAGTGTGGGCATCGCCGTCGCGGCCACTCTTATTGCCCGGTATCACCAGTTTTACCA
GAATGTCCTGGTGGAACAGGTAACGCCATACAATCTTGCGGCACAACAACGGCTCAACGTGCTCGCGGAAGGGATGCAAC
AGCGGGGAATGGATGGGGTGACCGCAGGGAAAGCTGCGCTCGGAGCACTCTACGGTGTTATTCAGAAACAGGCGGGGATG
CTGGCATACAACCGGATATTCTGGATCGTGGGGATCGCCTTTCTCAGCGTGATCCCTTTTCTGCTGCTTCTGCGACGCCC
ACGTCACACGGGCGAATCCGCGGGGACGCACTGA

Upstream 100 bases:

>100_bases
CCTGTTGCGGGTGGGCATGAGCGTGGTGCCGTCGGTGGACGTGGAGCGCTCTCTGGGCGACATCCTCCACGGGCTCTGGC
CGTTCTAGAAGGCAGGAGAC

Downstream 100 bases:

>100_bases
CCGCTCTCACGTCATCAGATCATCCGTCAGCTCGTTCGTATCGTCGGCCCTGCGCGGAAAATGCCTGGCCAGTTCGGCCC
CGCACCCGGCAACAGCGGCG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MEPDTKTVNKWLITITVMLPTIMEIVDTSVANVALPHMQGSLNAGTDEITWVLTSYLVSNAVVLPMTGWFSRLFGRKRFL
ISCITLFTIASFLCGAAPNLTSLIFFRIVQGAAGGALIPSSQAILMETFPPRERGMANAIFGIGAMFGPIIGPALGGWIT
DNLNWRWIFYINIPIGILAVIMAVYFIFDPPYLRRSKLSIDWWGLGLLTVGLGALQIVLDKGQQDDWFNSSFITGASIIS
VLALMLLIYVELRHEHPIVNLRLFKDISFSAGNLIMFMIGFCLYSSIMLIPLFLQTLMGYDATLAGMVLAPGGVATLLTM
PFVGAIISRYDGRKVVLGGLLIGATAMFLMQRFTLEASYWDFVWPRVVLGVGLAMLFVPLNTVTLMPIPKEEMGNATGML
NLMRNIGGSVGIAVAATLIARYHQFYQNVLVEQVTPYNLAAQQRLNVLAEGMQQRGMDGVTAGKAALGALYGVIQKQAGM
LAYNRIFWIVGIAFLSVIPFLLLLRRPRHTGESAGTH

Sequences:

>Translated_517_residues
MEPDTKTVNKWLITITVMLPTIMEIVDTSVANVALPHMQGSLNAGTDEITWVLTSYLVSNAVVLPMTGWFSRLFGRKRFL
ISCITLFTIASFLCGAAPNLTSLIFFRIVQGAAGGALIPSSQAILMETFPPRERGMANAIFGIGAMFGPIIGPALGGWIT
DNLNWRWIFYINIPIGILAVIMAVYFIFDPPYLRRSKLSIDWWGLGLLTVGLGALQIVLDKGQQDDWFNSSFITGASIIS
VLALMLLIYVELRHEHPIVNLRLFKDISFSAGNLIMFMIGFCLYSSIMLIPLFLQTLMGYDATLAGMVLAPGGVATLLTM
PFVGAIISRYDGRKVVLGGLLIGATAMFLMQRFTLEASYWDFVWPRVVLGVGLAMLFVPLNTVTLMPIPKEEMGNATGML
NLMRNIGGSVGIAVAATLIARYHQFYQNVLVEQVTPYNLAAQQRLNVLAEGMQQRGMDGVTAGKAALGALYGVIQKQAGM
LAYNRIFWIVGIAFLSVIPFLLLLRRPRHTGESAGTH
>Mature_517_residues
MEPDTKTVNKWLITITVMLPTIMEIVDTSVANVALPHMQGSLNAGTDEITWVLTSYLVSNAVVLPMTGWFSRLFGRKRFL
ISCITLFTIASFLCGAAPNLTSLIFFRIVQGAAGGALIPSSQAILMETFPPRERGMANAIFGIGAMFGPIIGPALGGWIT
DNLNWRWIFYINIPIGILAVIMAVYFIFDPPYLRRSKLSIDWWGLGLLTVGLGALQIVLDKGQQDDWFNSSFITGASIIS
VLALMLLIYVELRHEHPIVNLRLFKDISFSAGNLIMFMIGFCLYSSIMLIPLFLQTLMGYDATLAGMVLAPGGVATLLTM
PFVGAIISRYDGRKVVLGGLLIGATAMFLMQRFTLEASYWDFVWPRVVLGVGLAMLFVPLNTVTLMPIPKEEMGNATGML
NLMRNIGGSVGIAVAATLIARYHQFYQNVLVEQVTPYNLAAQQRLNVLAEGMQQRGMDGVTAGKAALGALYGVIQKQAGM
LAYNRIFWIVGIAFLSVIPFLLLLRRPRHTGESAGTH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=500, Percent_Identity=35, Blast_Score=315, Evalue=5e-87,
Organism=Escherichia coli, GI1788710, Length=507, Percent_Identity=34.1222879684418, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI1790195, Length=421, Percent_Identity=27.5534441805226, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI87081983, Length=415, Percent_Identity=26.2650602409639, Blast_Score=145, Evalue=6e-36,
Organism=Escherichia coli, GI1788392, Length=443, Percent_Identity=27.9909706546275, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI87082320, Length=196, Percent_Identity=23.9795918367347, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319770, Length=458, Percent_Identity=25.764192139738, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323735, Length=499, Percent_Identity=22.0440881763527, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6322958, Length=437, Percent_Identity=23.7986270022883, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6325455, Length=140, Percent_Identity=32.8571428571429, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324954, Length=399, Percent_Identity=24.0601503759398, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321663, Length=133, Percent_Identity=31.5789473684211, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56631; Mature: 56631

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPDTKTVNKWLITITVMLPTIMEIVDTSVANVALPHMQGSLNAGTDEITWVLTSYLVSN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHC
AVVLPMTGWFSRLFGRKRFLISCITLFTIASFLCGAAPNLTSLIFFRIVQGAAGGALIPS
CEEEECHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCC
SQAILMETFPPRERGMANAIFGIGAMFGPIIGPALGGWITDNLNWRWIFYINIPIGILAV
CCEEEEECCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEEEEECCHHHHHH
IMAVYFIFDPPYLRRSKLSIDWWGLGLLTVGLGALQIVLDKGQQDDWFNSSFITGASIIS
HHHHHHHCCCCHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
VLALMLLIYVELRHEHPIVNLRLFKDISFSAGNLIMFMIGFCLYSSIMLIPLFLQTLMGY
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DATLAGMVLAPGGVATLLTMPFVGAIISRYDGRKVVLGGLLIGATAMFLMQRFTLEASYW
CHHHHHHEECCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCHH
DFVWPRVVLGVGLAMLFVPLNTVTLMPIPKEEMGNATGMLNLMRNIGGSVGIAVAATLIA
HHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHH
RYHQFYQNVLVEQVTPYNLAAQQRLNVLAEGMQQRGMDGVTAGKAALGALYGVIQKQAGM
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCH
LAYNRIFWIVGIAFLSVIPFLLLLRRPRHTGESAGTH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MEPDTKTVNKWLITITVMLPTIMEIVDTSVANVALPHMQGSLNAGTDEITWVLTSYLVSN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHC
AVVLPMTGWFSRLFGRKRFLISCITLFTIASFLCGAAPNLTSLIFFRIVQGAAGGALIPS
CEEEECHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCC
SQAILMETFPPRERGMANAIFGIGAMFGPIIGPALGGWITDNLNWRWIFYINIPIGILAV
CCEEEEECCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCEECCCCEEEEEEEECCHHHHHH
IMAVYFIFDPPYLRRSKLSIDWWGLGLLTVGLGALQIVLDKGQQDDWFNSSFITGASIIS
HHHHHHHCCCCHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
VLALMLLIYVELRHEHPIVNLRLFKDISFSAGNLIMFMIGFCLYSSIMLIPLFLQTLMGY
HHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DATLAGMVLAPGGVATLLTMPFVGAIISRYDGRKVVLGGLLIGATAMFLMQRFTLEASYW
CHHHHHHEECCCHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCHH
DFVWPRVVLGVGLAMLFVPLNTVTLMPIPKEEMGNATGMLNLMRNIGGSVGIAVAATLIA
HHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHH
RYHQFYQNVLVEQVTPYNLAAQQRLNVLAEGMQQRGMDGVTAGKAALGALYGVIQKQAGM
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCH
LAYNRIFWIVGIAFLSVIPFLLLLRRPRHTGESAGTH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]