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The map label for this gene is ybjF [C]

Identifier: 39996552

GI number: 39996552

Start: 1589716

End: 1591203

Strand: Reverse

Name: ybjF [C]

Synonym: GSU1452

Alternate gene names: 39996552

Gene position: 1591203-1589716 (Counterclockwise)

Preceding gene: 39996580

Following gene: 39996548

Centisome position: 41.72

GC content: 60.42

Gene sequence:

>1488_bases
ATGAATAAAAAACGATCTCCCAAAACCGTACTCGCAGCCGGCCCCTCCTCCAAACGAGGTGATCTGATCGAGCTTGCCGT
TACCGCCCTCGACGAGGATGGAAACGGTATCGGCACCCATGACGGTACCAACGTCCATGTGATAGGGGCTTTACCTGATG
AACGGGTGCGGGCTCGCCTTACCCACGTGGGCAAAAGGCACCTCCACGCAGAAGCGGTCGAGGTGCTCACCCCATCGCGG
GCAAGACTTGCACAGCCCTCCTGTAAAAGGGCCGGCAGTTGCGACGGCTGTCCGCTGATCGTTATGCATTATCCGGACCA
GCTAAACTGGAAGCGCACCTTCACCGAGCGTCAGATCCGCCGCTACCAGACGTTGGGCGCTGCGGAGGTGCTGGCGACAC
TTCCCTCCCCCAATCAGCTTCACTATCGCAACTCGGCGAAACTGGTGGTTTCTGGGACGTTCCGCCGCCCGGTGATCGGC
ATTTACCGACGCAACAGCCATCAGGTCATGGATATCGGCACATGCCCCCTCCATCATCCTCTTATCAACCGAGTCGTTAC
AGCCGTGAAGGAGGGAATCGCCAAGTGCAAGGTCCAGGTCTACAATCCGCGGACCGGAAGCGGCCTTCTCCGTTATCTGG
TGGTACGGATTTCGGAACGGACGGGCACGGCCATGGCGGTGTTTGTCACGGTCGAGCGCAACTACAATGAAATCCACCAC
CTGGCGAAGCACCTGCAGCAGAGCGTCCCCCAGGTCGAGGTAGTTGTTCAGAACGTCAACTCATCGGAGGGGAATGTCAT
CCTCGGACAGCGGGACTACTTTCTGACCCGCCAGCATGCCCTTACGGAGGAGTTGGGCGGCATCCGCTTCACCATTTCAC
CCCGGTCCTTCTTTCAGGTAAACAGCGGCGGGGCCCGGATCATATACGAGACGGTCCGCCAATGGAGCAGCCTGACCGGC
AAGGAGTCCGTTGTCGATCTTTACTGCGGCATCGGTGGCATCGCACTCTTTCTCGCGGGCACGGCCCGCGAGGTTCACGG
CATTGAAGTTGTGGAAGCGGCGGTAAACGACGCCGAAAGCAACGCCCGGCTGAACCGCATCCACAACTGCAGCTTCGAGG
CGGGTGATGCAGCCGAACTGATTGAGGAACTCGTGGAGGAGGGTGAACGTCTTGACCTGGTCGTACTCAATCCACCGCGA
AAGGGATGTGACGCTGGCGTTCTCAATAAAGTCGCCGCAGCCGGACCTGCGCGGATAGTCTACGTTTCCTGCGCCCCTGC
CACCCTCGCCCGCGACCTGGACATCCTCGCCGGCCTGGGGTATGCCACCCTCCGGGTGCAACCCGTCGACATGTTCCCCC
AGACCCCCCACGTGGAGAACATAGCCTTGCTTGTCAAGAAGTTGCCGCGCAATGACCGGCTTGAGTCGCCGGCCAAAGAG
CGCAGCCGTCCCCGGGCCAGCCACAAGGCAAAAGGGGGAGCCGTCTGA

Upstream 100 bases:

>100_bases
AAATATTAGTAAAATTCTTCTCAGGATGCAACTCAAACCACTGTTCATCCACCGGCCTGAGTCATTGACAATCCCTGAAG
ATTCCTGTTTAACTGAAGCC

Downstream 100 bases:

>100_bases
ACGGCCCCCCCTTTTGGGAACATCGACAATGCAGCTTTCCGGAAATGCTGTTATTTTCTGAGCACCTTCACGACCGAAGA
GAAGTCTTCCTCACCCCGCC

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MNKKRSPKTVLAAGPSSKRGDLIELAVTALDEDGNGIGTHDGTNVHVIGALPDERVRARLTHVGKRHLHAEAVEVLTPSR
ARLAQPSCKRAGSCDGCPLIVMHYPDQLNWKRTFTERQIRRYQTLGAAEVLATLPSPNQLHYRNSAKLVVSGTFRRPVIG
IYRRNSHQVMDIGTCPLHHPLINRVVTAVKEGIAKCKVQVYNPRTGSGLLRYLVVRISERTGTAMAVFVTVERNYNEIHH
LAKHLQQSVPQVEVVVQNVNSSEGNVILGQRDYFLTRQHALTEELGGIRFTISPRSFFQVNSGGARIIYETVRQWSSLTG
KESVVDLYCGIGGIALFLAGTAREVHGIEVVEAAVNDAESNARLNRIHNCSFEAGDAAELIEELVEEGERLDLVVLNPPR
KGCDAGVLNKVAAAGPARIVYVSCAPATLARDLDILAGLGYATLRVQPVDMFPQTPHVENIALLVKKLPRNDRLESPAKE
RSRPRASHKAKGGAV

Sequences:

>Translated_495_residues
MNKKRSPKTVLAAGPSSKRGDLIELAVTALDEDGNGIGTHDGTNVHVIGALPDERVRARLTHVGKRHLHAEAVEVLTPSR
ARLAQPSCKRAGSCDGCPLIVMHYPDQLNWKRTFTERQIRRYQTLGAAEVLATLPSPNQLHYRNSAKLVVSGTFRRPVIG
IYRRNSHQVMDIGTCPLHHPLINRVVTAVKEGIAKCKVQVYNPRTGSGLLRYLVVRISERTGTAMAVFVTVERNYNEIHH
LAKHLQQSVPQVEVVVQNVNSSEGNVILGQRDYFLTRQHALTEELGGIRFTISPRSFFQVNSGGARIIYETVRQWSSLTG
KESVVDLYCGIGGIALFLAGTAREVHGIEVVEAAVNDAESNARLNRIHNCSFEAGDAAELIEELVEEGERLDLVVLNPPR
KGCDAGVLNKVAAAGPARIVYVSCAPATLARDLDILAGLGYATLRVQPVDMFPQTPHVENIALLVKKLPRNDRLESPAKE
RSRPRASHKAKGGAV
>Mature_495_residues
MNKKRSPKTVLAAGPSSKRGDLIELAVTALDEDGNGIGTHDGTNVHVIGALPDERVRARLTHVGKRHLHAEAVEVLTPSR
ARLAQPSCKRAGSCDGCPLIVMHYPDQLNWKRTFTERQIRRYQTLGAAEVLATLPSPNQLHYRNSAKLVVSGTFRRPVIG
IYRRNSHQVMDIGTCPLHHPLINRVVTAVKEGIAKCKVQVYNPRTGSGLLRYLVVRISERTGTAMAVFVTVERNYNEIHH
LAKHLQQSVPQVEVVVQNVNSSEGNVILGQRDYFLTRQHALTEELGGIRFTISPRSFFQVNSGGARIIYETVRQWSSLTG
KESVVDLYCGIGGIALFLAGTAREVHGIEVVEAAVNDAESNARLNRIHNCSFEAGDAAELIEELVEEGERLDLVVLNPPR
KGCDAGVLNKVAAAGPARIVYVSCAPATLARDLDILAGLGYATLRVQPVDMFPQTPHVENIALLVKKLPRNDRLESPAKE
RSRPRASHKAKGGAV

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI34222389, Length=383, Percent_Identity=29.7650130548303, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI51173878, Length=383, Percent_Identity=29.7650130548303, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI269784680, Length=204, Percent_Identity=32.3529411764706, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI269784676, Length=204, Percent_Identity=32.3529411764706, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI20149688, Length=204, Percent_Identity=32.3529411764706, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI269784678, Length=204, Percent_Identity=32.3529411764706, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1787083, Length=380, Percent_Identity=31.3157894736842, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1789148, Length=455, Percent_Identity=27.6923076923077, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1790403, Length=345, Percent_Identity=24.9275362318841, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322909, Length=435, Percent_Identity=24.8275862068966, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24666579, Length=485, Percent_Identity=27.2164948453608, Blast_Score=146, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1452_GEOSL (Q74D67)

Other databases:

- EMBL:   AE017180
- RefSeq:   NP_952503.1
- ProteinModelPortal:   Q74D67
- SMR:   Q74D67
- GeneID:   2687765
- GenomeReviews:   AE017180_GR
- KEGG:   gsu:GSU1452
- NMPDR:   fig|243231.1.peg.1441
- TIGR:   GSU1452
- HOGENOM:   HBG690163
- OMA:   RKGCDEA
- ProtClustDB:   CLSK2391137
- BioCyc:   GSUL243231:GSU_1452-MONOMER
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: 2.1.1.- [C]

Molecular weight: Translated: 54207; Mature: 54207

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 424-424 BINDING 299-299 BINDING 328-328 BINDING 349-349 BINDING 397-397

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKRSPKTVLAAGPSSKRGDLIELAVTALDEDGNGIGTHDGTNVHVIGALPDERVRARL
CCCCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHH
THVGKRHLHAEAVEVLTPSRARLAQPSCKRAGSCDGCPLIVMHYPDQLNWKRTFTERQIR
HHHHHHHHHHHHHHHCCCCHHHHCCCCHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHH
RYQTLGAAEVLATLPSPNQLHYRNSAKLVVSGTFRRPVIGIYRRNSHQVMDIGTCPLHHP
HHHHCCHHHHHHCCCCCCCEEECCCCEEEEEECCCCCEEEEEECCCCEEEEECCCCCCHH
LINRVVTAVKEGIAKCKVQVYNPRTGSGLLRYLVVRISERTGTAMAVFVTVERNYNEIHH
HHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCHHHHHH
LAKHLQQSVPQVEVVVQNVNSSEGNVILGQRDYFLTRQHALTEELGGIRFTISPRSFFQV
HHHHHHHHCCCEEEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHCCEEEEECCCCEEEE
NSGGARIIYETVRQWSSLTGKESVVDLYCGIGGIALFLAGTAREVHGIEVVEAAVNDAES
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHCCCHHHCCHHHHHHHHCCCCC
NARLNRIHNCSFEAGDAAELIEELVEEGERLDLVVLNPPRKGCDAGVLNKVAAAGPARIV
CCHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCEEE
YVSCAPATLARDLDILAGLGYATLRVQPVDMFPQTPHVENIALLVKKLPRNDRLESPAKE
EEEECCHHHHHHHHHHHCCCEEEEEEEEHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHH
RSRPRASHKAKGGAV
HCCCCHHHCCCCCCC
>Mature Secondary Structure
MNKKRSPKTVLAAGPSSKRGDLIELAVTALDEDGNGIGTHDGTNVHVIGALPDERVRARL
CCCCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCCEEEEECCCCHHHHHHH
THVGKRHLHAEAVEVLTPSRARLAQPSCKRAGSCDGCPLIVMHYPDQLNWKRTFTERQIR
HHHHHHHHHHHHHHHCCCCHHHHCCCCHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHH
RYQTLGAAEVLATLPSPNQLHYRNSAKLVVSGTFRRPVIGIYRRNSHQVMDIGTCPLHHP
HHHHCCHHHHHHCCCCCCCEEECCCCEEEEEECCCCCEEEEEECCCCEEEEECCCCCCHH
LINRVVTAVKEGIAKCKVQVYNPRTGSGLLRYLVVRISERTGTAMAVFVTVERNYNEIHH
HHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEEECCHHHHHH
LAKHLQQSVPQVEVVVQNVNSSEGNVILGQRDYFLTRQHALTEELGGIRFTISPRSFFQV
HHHHHHHHCCCEEEEEECCCCCCCCEEEECCCHHHHHHHHHHHHHCCEEEEECCCCEEEE
NSGGARIIYETVRQWSSLTGKESVVDLYCGIGGIALFLAGTAREVHGIEVVEAAVNDAES
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHCCCHHHCCHHHHHHHHCCCCC
NARLNRIHNCSFEAGDAAELIEELVEEGERLDLVVLNPPRKGCDAGVLNKVAAAGPARIV
CCHHHHHCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCEEE
YVSCAPATLARDLDILAGLGYATLRVQPVDMFPQTPHVENIALLVKKLPRNDRLESPAKE
EEEECCHHHHHHHHHHHCCCEEEEEEEEHHCCCCCCCHHHHHHHHHHCCCCCCCCCCHHH
RSRPRASHKAKGGAV
HCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA